FastQCFastQC Report
Wed 25 May 2016
SRR1295365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295365_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5385368
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT236110.4384287201914521No Hit
TATCAACGCAGAGTACTTTTTTTTT157970.2933318577300567No Hit
GGTATCAACGCAGAGTACTTTTTTT141730.26317607264721743No Hit
GTACTTTTTTTTTTTTTTTTTTTTT139700.2594065995118625No Hit
GAGTACTTTTTTTTTTTTTTTTTTT68320.12686226827953076No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58800.10918473909303876No Hit
GGTATCAACGCAGAGTACATGGGGG56390.10470965029687851No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGA350.00217113416.28571510
CCGATCG400.005281339414.259
CGCGATT400.005281339414.2514
ATAACGC3700.012.8378383
GGTATCA41250.012.7357571
TAATACG751.4801863E-512.6666674
TATAACG3300.012.3787882
GTGGTAT12700.011.9685041
AAACGCG1408.36917E-910.85714311
TACGACC2050.010.6585374
GTCACGC1701.4006218E-1010.6176478
GCGAATT1451.4462785E-810.482758514
TATCACG4100.010.1951232
TGGTATC14500.010.1551732
CGTAATA856.598568E-410.0588242
GTATCAA86150.010.0348221
ACGATTT2201.8189894E-129.93181815
CACGATT2300.09.91304314
TAACGCA4050.09.8518524
TAACGCT1304.243946E-69.54
Produced by FastQC (version 0.11.5)