##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295365_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5385368 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.62704888505298 33.0 32.0 34.0 32.0 34.0 2 32.80538563009993 33.0 32.0 34.0 32.0 34.0 3 32.93462675159803 33.0 32.0 34.0 32.0 35.0 4 33.0285791054576 33.0 33.0 34.0 32.0 35.0 5 32.802299118648904 33.0 32.0 34.0 32.0 35.0 6 35.60745226695743 37.0 35.0 37.0 33.0 37.0 7 35.56443106580646 37.0 35.0 37.0 33.0 37.0 8 35.50207655261442 37.0 35.0 37.0 33.0 37.0 9 35.46467353763011 37.0 34.0 37.0 33.0 37.0 10 35.351975203922926 37.0 34.0 37.0 33.0 37.0 11 35.29297645026301 37.0 34.0 37.0 33.0 37.0 12 35.25926436224971 37.0 34.0 37.0 33.0 37.0 13 36.95208795387799 38.0 38.0 38.0 36.0 38.0 14 36.957661760533355 38.0 38.0 38.0 36.0 38.0 15 36.83912594273966 38.0 38.0 38.0 36.0 38.0 16 36.96419631861741 38.0 38.0 38.0 36.0 38.0 17 36.955806548410436 38.0 38.0 38.0 36.0 38.0 18 37.02150178037972 38.0 38.0 38.0 36.0 38.0 19 36.98593522299683 38.0 38.0 38.0 36.0 38.0 20 36.992752584410205 38.0 38.0 38.0 36.0 38.0 21 36.96550133621324 38.0 38.0 38.0 36.0 38.0 22 36.99866694346607 38.0 38.0 38.0 36.0 38.0 23 37.57153605844577 39.0 38.0 39.0 36.0 39.0 24 37.5455101675503 39.0 38.0 39.0 36.0 39.0 25 37.43543096776302 39.0 38.0 39.0 36.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05891402818470226 1101 2 0.19407117648766814 1101 3 0.20356499622970858 1101 4 0.06506455539101097 1101 5 0.18566084263444083 1101 6 0.03053530206511823 1101 7 0.045827225800330496 1101 8 0.10099532607793549 1101 9 -0.05244283085639978 1101 10 0.14024596157172908 1101 11 0.1138448775341061 1101 12 0.08157495011240457 1101 13 0.12546750819105768 1101 14 0.11724745969883799 1101 15 0.3513047677484096 1101 16 0.12022230114632748 1101 17 0.18448245827948284 1101 18 0.026126390819939616 1101 19 0.1264206404228787 1101 20 0.1410627362684167 1101 21 0.19361998920987844 1101 22 0.11580874134597963 1101 23 0.12142202185812323 1101 24 0.04141220312361327 1101 25 0.19271460430093867 1102 1 -0.1407341115711347 1102 2 -0.08552815863024676 1102 3 0.04156521985673578 1102 4 0.06886139314274686 1102 5 0.17890568433735154 1102 6 0.049381762874659785 1102 7 0.14581885173251408 1102 8 0.12126300824358793 1102 9 0.10631421508654171 1102 10 0.004619153038873947 1102 11 0.0726163693141757 1102 12 0.12403044768684168 1102 13 0.11495546570922244 1102 14 0.13074070724736941 1102 15 0.2701687303705498 1102 16 -0.05396854491264236 1102 17 0.09481320398495541 1102 18 -0.1294740626937667 1102 19 0.03792029946006892 1102 20 0.0481045128105535 1102 21 0.1740797030530743 1102 22 0.19864274559117945 1102 23 0.09892908945298728 1102 24 0.1427993260270739 1102 25 0.04988635808258124 1103 1 0.1091248946627914 1103 2 0.08712045497426857 1103 3 0.07897367224842355 1103 4 0.05498960411864573 1103 5 0.18050598336017032 1103 6 0.16737275641439453 1103 7 0.09657291126958967 1103 8 0.06420641748603373 1103 9 0.05147712243953606 1103 10 -0.003283969476406412 1103 11 -0.07183900688404776 1103 12 0.1121297255816387 1103 13 0.10440367772762471 1103 14 0.061319363155732276 1103 15 0.12991011624697535 1103 16 -0.018591415815578216 1103 17 0.09075275492948265 1103 18 0.10180813584558734 1103 19 0.13666656689404277 1103 20 0.10197641566690407 1103 21 0.11017750895186396 1103 22 0.24862803530018596 1103 23 0.08295865568751282 1103 24 0.008289075652825773 1103 25 -0.10171840852636649 1104 1 0.03406534773169767 1104 2 0.14939166578626129 1104 3 0.06117628975223255 1104 4 0.09259152449955366 1104 5 0.06302351662321115 1104 6 0.09397437982288892 1104 7 0.07643366962857101 1104 8 0.04208799501321181 1104 9 0.13827226270258564 1104 10 0.11968250130230729 1104 11 0.10938779405235977 1104 12 0.1338882138396471 1104 13 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0.02677958790031454 2110 9 -0.06117091724230761 2110 10 -0.05747286608622204 2110 11 0.02180333764300002 2110 12 -0.10603499452249565 2110 13 -0.2766038831615987 2110 14 -0.21213954468193208 2110 15 0.015916318718716127 2110 16 -0.10369967483872244 2110 17 -0.16674223098683427 2110 18 -0.043411349789757026 2110 19 -0.00670502088294711 2110 20 -0.05571326271472543 2110 21 -0.2208729018391722 2110 22 -0.49933214353468713 2110 23 -0.14531903588693496 2110 24 -0.02630924359874598 2110 25 -0.08331617487740317 2111 1 0.0719235343285547 2111 2 -0.021115238569379358 2111 3 -0.13435289543802043 2111 4 -0.04736333310387408 2111 5 -0.13009274529930082 2111 6 -0.03173258053021755 2111 7 -0.048473133875830854 2111 8 -0.34010355254367397 2111 9 -0.10024590833059932 2111 10 -0.2274424982562664 2111 11 -0.11094419888491558 2111 12 0.03758017821664339 2111 13 -0.7212011126236177 2111 14 -0.18644009561120356 2111 15 0.15018535915746867 2111 16 -0.1442122955789813 2111 17 -0.02210844481978569 2111 18 -0.31941507745075626 2111 19 -0.0030225425740226797 2111 20 0.03936453995323319 2111 21 -0.0011321468652099043 2111 22 -0.0366020682537922 2111 23 0.07404322525923135 2111 24 0.05947341819052809 2111 25 0.11412589435149556 2112 1 -0.004235329070176874 2112 2 0.10463749908780073 2112 3 0.006079571561137698 2112 4 -0.06539644382743148 2112 5 -0.30024463428543413 2112 6 0.03790913460826317 2112 7 0.04672865428564421 2112 8 -0.005989256474556726 2112 9 -0.013864041488240275 2112 10 0.021656538110335077 2112 11 -0.054899959789409536 2112 12 -0.01549164708878692 2112 13 0.09672714616959865 2112 14 -0.13380417177231863 2112 15 0.05880071467996828 2112 16 -0.28605192222944 2112 17 -0.17209843627089327 2112 18 -0.4459552477368618 2112 19 -0.038383120983226604 2112 20 -0.007942279147705733 2112 21 -0.012595549709573106 2112 22 -0.06745153291419115 2112 23 -0.027470908483572032 2112 24 -0.11751529606835476 2112 25 0.10806821897672592 2113 1 0.06138753837102229 2113 2 -0.07259171414649757 2113 3 -0.07199765497443877 2113 4 -0.13603942079455322 2113 5 -0.1547536502371969 2113 6 0.022900998485866353 2113 7 -0.2157207254764799 2113 8 -0.033609684394427575 2113 9 -0.11683169049496911 2113 10 0.006447684305861401 2113 11 -0.21132381603759143 2113 12 -0.007923903486457107 2113 13 0.09622905850478247 2113 14 0.10516851114228132 2113 15 0.049092358803392244 2113 16 -0.059082641322454776 2113 17 -0.05950262526435779 2113 18 -0.12085363287923201 2113 19 0.003384668288362036 2113 20 -0.24983572652858754 2113 21 0.015321159130529338 2113 22 0.08544182765056973 2113 23 0.15901966836711523 2113 24 -0.2084651535960731 2113 25 0.12303755891143453 2114 1 -0.3573477651018848 2114 2 -0.05634537769837067 2114 3 -0.05800217439472277 2114 4 -0.02409357220036412 2114 5 -0.12063856135309692 2114 6 0.10365380345002251 2114 7 0.024779014655692322 2114 8 -0.049478008976649335 2114 9 -0.0428504646385619 2114 10 -0.014600947655637242 2114 11 -0.16330239332372543 2114 12 -8.168404632371562E-4 2114 13 0.058952360444486374 2114 14 0.02679202002777714 2114 15 -0.036236339155664155 2114 16 -0.06778112641482181 2114 17 0.039637499352558336 2114 18 -0.12914753273058466 2114 19 -0.05469365726627018 2114 20 -0.4019341612098799 2114 21 -0.010430095109036586 2114 22 0.08175652121146726 2114 23 -0.053685001878108096 2114 24 -0.16462540423055572 2114 25 0.14127324284122267 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 1.0 16 4.0 17 8.0 18 9.0 19 11.0 20 18.0 21 80.0 22 309.0 23 981.0 24 2682.0 25 6302.0 26 12814.0 27 23541.0 28 38369.0 29 58433.0 30 81026.0 31 108799.0 32 143095.0 33 196242.0 34 305690.0 35 716750.0 36 2853384.0 37 836819.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.53290248688669 18.38873406608425 12.925894757795566 25.152468689233494 2 17.55716600982514 20.038017829050865 37.3314692700666 25.073346891057398 3 18.73209036039877 24.724308533790076 29.582360945435855 26.9612401603753 4 14.15494725708624 17.167777578059663 35.79699660264628 32.88027856220782 5 14.377123346074027 35.69314111867564 33.41225706395552 16.51747847129481 6 31.38806855910311 35.66068279827859 18.247146713093702 14.704101929524594 7 28.11889549609237 30.70870922841299 22.50605715338302 18.66633812211162 8 26.34555335865627 33.25419915593512 20.75941699805844 19.64083048735017 9 26.170189298112962 16.240672875094145 20.57031200096261 37.018825825830284 10 16.969518146206536 26.934018250934756 31.99424811823445 24.102215484624264 11 33.57369449961451 22.72760190204272 24.076664770169838 19.62203882817293 12 24.0424238417876 24.34221765346398 29.566373180068663 22.048985324679762 13 28.195881878452873 21.15173930546622 26.169075168122212 24.48330364795869 14 23.202054158601605 20.544371341011423 26.663897434678557 29.589677065708415 15 24.206646602423458 27.6312965056427 23.843997290435862 24.318059601497986 16 23.942894895947685 26.996929457745505 24.699871949326397 24.36030369698041 17 23.02479607707403 26.569920569959194 26.45633501740271 23.94894833556407 18 22.8852327269 26.345256257325406 27.529260767323606 23.240250248450987 19 23.971249504212157 25.545236648637566 27.655770227772734 22.827743619377543 20 24.322516121460964 25.70702689212696 27.151292167963266 22.819164818448805 21 24.531842577888828 25.153044323062048 26.994534078265403 23.320579020783725 22 24.205142526935948 25.179764874006754 27.11939091256159 23.495701686495703 23 24.187260740584488 25.33624443120693 26.803497922518943 23.67299690568964 24 23.443616109428362 25.592178658914307 27.119632307392923 23.844572924264416 25 23.90247054611681 24.93257656672673 27.457696484251404 23.70725640290506 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1444.0 1 1444.0 2 2123.0 3 2802.0 4 2802.0 5 2802.0 6 9823.5 7 16845.0 8 16845.0 9 16845.0 10 16492.0 11 16139.0 12 16139.0 13 16139.0 14 19606.5 15 23074.0 16 23074.0 17 23074.0 18 39806.5 19 56539.0 20 56539.0 21 56539.0 22 85827.5 23 115116.0 24 115116.0 25 115116.0 26 162057.0 27 208998.0 28 208998.0 29 208998.0 30 256469.5 31 303941.0 32 303941.0 33 303941.0 34 352056.0 35 400171.0 36 400171.0 37 400171.0 38 448703.5 39 497236.0 40 497236.0 41 497236.0 42 547760.5 43 598285.0 44 598285.0 45 598285.0 46 617455.0 47 636625.0 48 636625.0 49 636625.0 50 645308.0 51 653991.0 52 653991.0 53 653991.0 54 640285.0 55 626579.0 56 626579.0 57 626579.0 58 564102.0 59 501625.0 60 501625.0 61 501625.0 62 428580.5 63 355536.0 64 355536.0 65 355536.0 66 278586.5 67 201637.0 68 201637.0 69 201637.0 70 151270.0 71 100903.0 72 100903.0 73 100903.0 74 72380.5 75 43858.0 76 43858.0 77 43858.0 78 30357.0 79 16856.0 80 16856.0 81 16856.0 82 11198.0 83 5540.0 84 5540.0 85 5540.0 86 3386.5 87 1233.0 88 1233.0 89 1233.0 90 774.0 91 315.0 92 315.0 93 315.0 94 191.5 95 68.0 96 68.0 97 68.0 98 40.0 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5385368.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.44714556268144 #Duplication Level Percentage of deduplicated Percentage of total 1 69.93971188155473 21.99404300151252 2 12.583811977968093 7.914499340091538 3 5.0233979122906645 4.739145761012238 4 2.6847010270087566 3.3770473595449886 5 1.711498479960981 2.6910870914820495 6 1.222701444812675 2.307028218883506 7 0.8974274804748449 1.9755072827310032 8 0.7151615423983363 1.799183129970581 9 0.5446916840356129 1.5416098807185004 >10 3.9835975425891528 24.685993445348473 >50 0.44128648512937046 9.5795136335768 >100 0.24286565149934736 13.516868202285817 >500 0.007494549628460309 1.538095221142042 >1k 0.0012392554865761328 0.7462398900642199 >5k 1.770364980821007E-4 0.34055128376007304 >10k+ 2.360486641094676E-4 1.253587257875685 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 23611 0.4384287201914521 No Hit TATCAACGCAGAGTACTTTTTTTTT 15797 0.2933318577300567 No Hit GGTATCAACGCAGAGTACTTTTTTT 14173 0.26317607264721743 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 13970 0.2594065995118625 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 6832 0.12686226827953076 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 5880 0.10918473909303876 No Hit GGTATCAACGCAGAGTACATGGGGG 5639 0.10470965029687851 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.8568833179088225E-5 0.0 5 0.0 1.8568833179088225E-5 0.0 1.8568833179088225E-5 0.0 6 0.0 1.8568833179088225E-5 0.0 1.8568833179088225E-5 0.0 7 0.0 1.8568833179088225E-5 0.0 1.8568833179088225E-5 0.0 8 0.0 1.8568833179088225E-5 0.0 3.713766635817645E-5 0.0 9 0.0 1.8568833179088225E-5 0.0 7.42753327163529E-5 0.0 10 0.0 1.8568833179088225E-5 0.0 1.2998183225361757E-4 0.0 11 0.0 1.8568833179088225E-5 0.0 1.8568833179088225E-4 0.0 12 0.0 1.8568833179088225E-5 0.0 2.2282599814905872E-4 0.0 13 0.0 1.8568833179088225E-5 0.0 2.7853249768632337E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGCGA 35 0.002171134 16.285715 10 CCGATCG 40 0.0052813394 14.25 9 CGCGATT 40 0.0052813394 14.25 14 ATAACGC 370 0.0 12.837838 3 GGTATCA 4125 0.0 12.735757 1 TAATACG 75 1.4801863E-5 12.666667 4 TATAACG 330 0.0 12.378788 2 GTGGTAT 1270 0.0 11.968504 1 AAACGCG 140 8.36917E-9 10.857143 11 TACGACC 205 0.0 10.658537 4 GTCACGC 170 1.4006218E-10 10.617647 8 GCGAATT 145 1.4462785E-8 10.4827585 14 TATCACG 410 0.0 10.195123 2 TGGTATC 1450 0.0 10.155173 2 CGTAATA 85 6.598568E-4 10.058824 2 GTATCAA 8615 0.0 10.034822 1 ACGATTT 220 1.8189894E-12 9.931818 15 CACGATT 230 0.0 9.913043 14 TAACGCA 405 0.0 9.851852 4 TAACGCT 130 4.243946E-6 9.5 4 >>END_MODULE