FastQCFastQC Report
Wed 25 May 2016
SRR1295364_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295364_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3646570
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACATGGGGG38250.10489309131594897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC1207.421477E-1012.6666661
GTGGTAT6850.011.9270071
CGTACCC1104.9746086E-711.2272733
ACGATTT1701.0913936E-1111.17647115
AGTTACG600.005878231511.08333318
GGTATCA19550.010.8849111
ATTCGCG700.001492237110.8571435
TCGTACC700.001492237110.8571432
GTATAAC2250.010.1333341
TATAACG1606.182745E-910.093752
GTATTAA7550.09.9403971
ACACCGT1752.577508E-99.7714286
CGCACGA2054.1836756E-119.73170712
TGGTATC8250.09.5575752
TGTCACG1804.1891326E-99.57
GTATTGG4600.09.51
ATTAGAC1401.0596759E-69.53
TAGCGTC1002.757124E-49.519
CGTTGGA1401.0596759E-69.52
CACGATT2002.6557245E-109.514
Produced by FastQC (version 0.11.5)