##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295364_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3646570 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.335596738853223 32.0 32.0 33.0 29.0 34.0 2 31.71262254666714 33.0 32.0 33.0 29.0 34.0 3 31.74451799910601 33.0 32.0 34.0 29.0 34.0 4 31.957540647786825 33.0 32.0 34.0 30.0 34.0 5 31.945785765801837 33.0 32.0 34.0 30.0 34.0 6 34.7053118409903 37.0 34.0 37.0 32.0 37.0 7 34.816113772668565 37.0 34.0 37.0 32.0 37.0 8 34.72470239156248 37.0 34.0 37.0 32.0 37.0 9 34.678008100763186 37.0 34.0 37.0 32.0 37.0 10 34.64329383502854 37.0 34.0 37.0 32.0 37.0 11 34.63007840244394 37.0 33.0 37.0 32.0 37.0 12 34.58685970651873 37.0 34.0 37.0 32.0 37.0 13 36.26924671677769 38.0 37.0 38.0 33.0 38.0 14 36.29304853602152 38.0 38.0 38.0 33.0 38.0 15 36.30576212714962 38.0 38.0 38.0 33.0 38.0 16 36.25732208623446 38.0 37.0 38.0 33.0 38.0 17 36.287232111271685 38.0 38.0 38.0 33.0 38.0 18 36.16661246047656 38.0 37.0 38.0 33.0 38.0 19 36.24608193453026 38.0 37.0 38.0 33.0 38.0 20 36.29167162566466 38.0 38.0 38.0 33.0 38.0 21 36.26146570612932 38.0 37.0 38.0 33.0 38.0 22 36.27964936913319 38.0 38.0 38.0 33.0 38.0 23 36.77698247942587 39.0 37.0 39.0 33.0 39.0 24 36.77608245556784 39.0 37.0 39.0 33.0 39.0 25 36.62854326120162 38.0 37.0 39.0 33.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18548021203933018 1101 2 -0.02158081776738996 1101 3 0.0653665671100967 1101 4 0.03095235132794727 1101 5 0.08760124588054197 1101 6 0.09588749923489104 1101 7 0.29263502680080933 1101 8 0.22447800696002673 1101 9 0.22673232784242003 1101 10 0.24298033310814304 1101 11 0.10846420060036621 1101 12 0.1718458962112095 1101 13 0.19557309553866276 1101 14 0.13224025787388882 1101 15 0.11145691983003303 1101 16 0.27749762474489614 1101 17 0.1390705685142848 1101 18 0.1926788523398173 1101 19 0.16302444197115307 1101 20 0.22931612513258415 1101 21 0.20743879847379532 1101 22 0.20766916395827906 1101 23 0.2891164281622167 1101 24 0.26461571866142464 1101 25 0.17553457064809663 1102 1 0.15285974335924735 1102 2 0.11606658474238785 1102 3 0.2405488583858002 1102 4 0.08829757197455024 1102 5 0.2144232369762733 1102 6 0.16593718766007726 1102 7 0.2853167697435737 1102 8 0.3335031740427823 1102 9 0.3880475033973454 1102 10 0.25145127750314344 1102 11 0.2625073354142273 1102 12 0.328674424661493 1102 13 0.38679801180518325 1102 14 0.28172721012614943 1102 15 0.30721027998497163 1102 16 0.4080635927286451 1102 17 0.2974103327484414 1102 18 0.3962677436163844 1102 19 0.29494440810375977 1102 20 0.33920306771961606 1102 21 0.336169194738396 1102 22 0.35962733439446026 1102 23 0.21299231454496947 1102 24 0.3507088315908291 1102 25 0.3266707968933318 1103 1 -0.1726951394503402 1103 2 0.08225434438677937 1103 3 0.0973728782414689 1103 4 -0.05739824155668316 1103 5 0.01735568020842848 1103 6 0.14429244753988968 1103 7 0.28390803832454736 1103 8 0.2676978107484018 1103 9 0.3735509310014109 1103 10 0.276095978768339 1103 11 0.23182009945077198 1103 12 0.15295775298267245 1103 13 0.24898963659165219 1103 14 0.23655718463374598 1103 15 0.16413358522165566 1103 16 0.36553273123463015 1103 17 0.18818988490410504 1103 18 0.2645826054143541 1103 19 0.1973714519657861 1103 20 0.2763473456377099 1103 21 0.21779181698415329 1103 22 0.13707342604264028 1103 23 0.061995067445955954 1103 24 0.1620465454275859 1103 25 0.10305705861210868 1104 1 0.04497540169806058 1104 2 0.10830573562369139 1104 3 0.05286563507905129 1104 4 0.11691686016046532 1104 5 0.1727865292313524 1104 6 0.16969189047222244 1104 7 0.32354702324126805 1104 8 0.22951917581858794 1104 9 0.3338338970875867 1104 10 0.23878994584148217 1104 11 0.2149664293952469 1104 12 0.0995745122910634 1104 13 0.10610884743167759 1104 14 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-0.030472495309844305 2110 14 0.06365133414241342 2110 15 0.021799728648652206 2110 16 0.09140369640562795 2110 17 -0.538161397400323 2110 18 0.00197583056023376 2110 19 0.07554636337553688 2110 20 -0.05462766865798585 2110 21 0.0480839298561051 2110 22 0.1798875505082762 2110 23 -0.0487379179638765 2110 24 -0.14093162547514737 2110 25 0.21073133121763732 2111 1 0.2388096908867503 2111 2 0.18089694783056487 2111 3 0.14748567129953116 2111 4 0.0635539586623679 2111 5 0.04397718214338653 2111 6 0.022839578287019435 2111 7 0.06993864381752957 2111 8 0.06258901236199677 2111 9 -0.1571125839172609 2111 10 0.0039786989739027945 2111 11 -0.014179461887565026 2111 12 -0.23916776865387845 2111 13 -0.1759836024954069 2111 14 -0.25005871657887724 2111 15 -0.17805437927824386 2111 16 -0.14271570047953475 2111 17 -0.7842419978280688 2111 18 -0.3460920757575323 2111 19 0.01153102117293514 2111 20 0.09016019089352056 2111 21 0.08042437994983231 2111 22 0.09597195416823467 2111 23 0.0033960803092298875 2111 24 0.010320584214390749 2111 25 0.1905725417588684 2112 1 0.04868852764516163 2112 2 -0.06355750213918654 2112 3 0.02652950264422671 2112 4 -0.06897231658827252 2112 5 -0.09927752328579231 2112 6 -0.10246590748190698 2112 7 -0.08736365832643145 2112 8 0.1040123695691193 2112 9 0.06974509529918294 2112 10 0.1439460233981933 2112 11 0.10446139151343914 2112 12 -0.15000319898800996 2112 13 0.028716858456647287 2112 14 -0.3108439466174602 2112 15 -0.3219390369515054 2112 16 -0.011574102926608987 2112 17 0.04250734214589613 2112 18 -0.34379313261872113 2112 19 0.07860863597899481 2112 20 0.07828252108902944 2112 21 -0.16817472022707136 2112 22 0.05828648638141942 2112 23 -0.08554684418912473 2112 24 -0.13121167817723745 2112 25 -0.21944146546112364 2113 1 0.033066326782606126 2113 2 -0.1465484175463132 2113 3 0.03846190797359483 2113 4 -0.02237295667463357 2113 5 -0.31107971766146747 2113 6 -0.14651359948711473 2113 7 -0.14091296049581103 2113 8 -0.10162241648752257 2113 9 0.03563264917077191 2113 10 0.24781211087811528 2113 11 0.13640651341480492 2113 12 -0.041730293799346896 2113 13 0.09825421353877317 2113 14 -0.18074519573790582 2113 15 -0.18698604936079732 2113 16 -0.01658220874136873 2113 17 0.0027197498557640643 2113 18 -0.06422447010384502 2113 19 0.0076223521116176585 2113 20 -0.1342577437734036 2113 21 -0.14325901915643158 2113 22 0.058055208094828004 2113 23 -0.29578343910446137 2113 24 -0.13806211875016317 2113 25 -0.5663042304974013 2114 1 0.10447255704471914 2114 2 0.044847506479335664 2114 3 -0.03318065254116931 2114 4 -0.0605301540495482 2114 5 0.04783974499718724 2114 6 -0.04787706655913837 2114 7 0.007757530339283392 2114 8 0.15993387649300672 2114 9 0.2736555531614897 2114 10 0.3442157424397152 2114 11 -0.11997918128659535 2114 12 0.1657007777089632 2114 13 0.19659020617012146 2114 14 0.11712391645743736 2114 15 0.032639569957318315 2114 16 0.19657695805231867 2114 17 0.29168930334074616 2114 18 0.16666657419153097 2114 19 7.552568807867033E-4 2114 20 -0.0049207689964134715 2114 21 0.11819740448162719 2114 22 0.16371417561596502 2114 23 0.18425481190250537 2114 24 0.2730223527673772 2114 25 -0.043729796955844336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 4.0 15 49.0 16 378.0 17 1316.0 18 2849.0 19 4936.0 20 7807.0 21 11181.0 22 14738.0 23 19469.0 24 23951.0 25 29311.0 26 35606.0 27 42119.0 28 50005.0 29 59780.0 30 70366.0 31 85285.0 32 107615.0 33 146678.0 34 237757.0 35 578413.0 36 1867185.0 37 249771.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.64484981777396 18.154786552842808 12.898586891243003 25.301776738140227 2 17.66553226730873 19.855370937620833 37.119292924583924 25.359803870486513 3 18.728037580520873 24.265295880786603 29.574586529259 27.432080009433523 4 14.382364797604325 16.934571391746218 35.739256342261356 32.9438074683881 5 15.144121736316594 35.10841695072356 33.48064071168248 16.266820601277367 6 31.692110668381517 35.043232407440414 18.231982383445263 15.032674540732799 7 28.64264226382601 30.524355764458104 22.073400483193794 18.75960148852209 8 26.6654966173692 32.76810811255508 20.735924444066615 19.8304708260091 9 26.474029019050775 16.392664887826093 20.205837266252942 36.92746882687018 10 17.4293925524534 26.787254872386928 31.57325925458719 24.210093320572483 11 33.44104185577132 22.770411647109476 23.897333658753293 19.891212838365917 12 23.892781435705334 24.51975417995541 28.84466772885205 22.74279665548721 13 28.2157479494429 21.237135170859194 25.491297301299575 25.05581957839833 14 23.34363525175713 20.81687722983516 26.002215780857078 29.83727173755063 15 24.58397891717422 27.64965433270169 23.09194667865967 24.674420071464418 16 24.405016220722487 26.972661980984867 24.18601589987303 24.43630589841961 17 23.47902275288833 26.821259430094578 25.659756977104514 24.039960839912574 18 23.4299081054251 26.87210172847361 26.387207704774625 23.31078246132667 19 24.479469748284004 26.10107580548296 26.160666050562583 23.25878839567045 20 24.7274836353066 26.110865827339115 25.82421289046967 23.337437646884606 21 24.9477728385853 25.647690843724373 25.573292162223677 23.831244155466642 22 24.725810830451632 25.77479658967194 25.429156714391883 24.070235865484552 23 24.846856086678713 25.68254551537472 25.331421034012784 24.13917736393378 24 24.056798580583948 26.017051640308566 25.644262964923204 24.28188681418429 25 24.53395930970748 25.45498920903753 25.76100280537601 24.250048675878976 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1311.0 1 1311.0 2 1196.5 3 1082.0 4 1082.0 5 1082.0 6 2000.0 7 2918.0 8 2918.0 9 2918.0 10 4759.5 11 6601.0 12 6601.0 13 6601.0 14 11234.5 15 15868.0 16 15868.0 17 15868.0 18 26218.0 19 36568.0 20 36568.0 21 36568.0 22 55194.5 23 73821.0 24 73821.0 25 73821.0 26 99764.0 27 125707.0 28 125707.0 29 125707.0 30 154713.5 31 183720.0 32 183720.0 33 183720.0 34 219878.5 35 256037.0 36 256037.0 37 256037.0 38 294960.0 39 333883.0 40 333883.0 41 333883.0 42 374043.5 43 414204.0 44 414204.0 45 414204.0 46 428092.0 47 441980.0 48 441980.0 49 441980.0 50 451888.5 51 461797.0 52 461797.0 53 461797.0 54 445890.5 55 429984.0 56 429984.0 57 429984.0 58 386393.0 59 342802.0 60 342802.0 61 342802.0 62 293631.0 63 244460.0 64 244460.0 65 244460.0 66 193591.5 67 142723.0 68 142723.0 69 142723.0 70 109494.0 71 76265.0 72 76265.0 73 76265.0 74 55219.0 75 34173.0 76 34173.0 77 34173.0 78 23811.0 79 13449.0 80 13449.0 81 13449.0 82 9250.5 83 5052.0 84 5052.0 85 5052.0 86 3276.0 87 1500.0 88 1500.0 89 1500.0 90 984.5 91 469.0 92 469.0 93 469.0 94 308.5 95 148.0 96 148.0 97 148.0 98 98.0 99 48.0 100 48.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3646570.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.63016182748349 #Duplication Level Percentage of deduplicated Percentage of total 1 74.686596583864 28.851553123786417 2 11.225204663549428 8.672629453990734 3 4.152460930707984 4.8123071320665645 4 2.2073310770070624 3.4107822684646494 5 1.4300957743138591 2.7622415595272343 6 0.9911005430075547 2.2971824618213152 7 0.703773478760212 1.9030818362078605 8 0.5685100245934228 1.7569307400472431 9 0.44406884705101696 1.5439006281712317 >10 3.115469043161809 23.367062245577934 >50 0.3089748601033849 8.21958900825072 >100 0.16228647281858574 10.832284959188751 >500 0.00291785764861842 0.7026412411764511 >1k 0.0012098434132377467 0.8678133417229417 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACATGGGGG 3825 0.10489309131594897 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.742303040939842E-5 0.0 6 0.0 2.742303040939842E-5 0.0 2.742303040939842E-5 0.0 7 0.0 2.742303040939842E-5 0.0 2.742303040939842E-5 0.0 8 0.0 2.742303040939842E-5 0.0 5.484606081879684E-5 0.0 9 0.0 2.742303040939842E-5 0.0 5.484606081879684E-5 0.0 10 0.0 2.742303040939842E-5 0.0 1.371151520469921E-4 0.0 11 0.0 2.742303040939842E-5 0.0 2.1938424327518736E-4 0.0 12 0.0 2.742303040939842E-5 0.0 3.2907636491278107E-4 0.0 13 0.0 2.742303040939842E-5 0.0 4.387684865503747E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGTAC 120 7.421477E-10 12.666666 1 GTGGTAT 685 0.0 11.927007 1 CGTACCC 110 4.9746086E-7 11.227273 3 ACGATTT 170 1.0913936E-11 11.176471 15 AGTTACG 60 0.0058782315 11.083333 18 GGTATCA 1955 0.0 10.884911 1 ATTCGCG 70 0.0014922371 10.857143 5 TCGTACC 70 0.0014922371 10.857143 2 GTATAAC 225 0.0 10.133334 1 TATAACG 160 6.182745E-9 10.09375 2 GTATTAA 755 0.0 9.940397 1 ACACCGT 175 2.577508E-9 9.771428 6 CGCACGA 205 4.1836756E-11 9.731707 12 TGGTATC 825 0.0 9.557575 2 TGTCACG 180 4.1891326E-9 9.5 7 GTATTGG 460 0.0 9.5 1 ATTAGAC 140 1.0596759E-6 9.5 3 TAGCGTC 100 2.757124E-4 9.5 19 CGTTGGA 140 1.0596759E-6 9.5 2 CACGATT 200 2.6557245E-10 9.5 14 >>END_MODULE