FastQCFastQC Report
Wed 25 May 2016
SRR1295364_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295364_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3646570
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT182120.499428229815964No Hit
TATCAACGCAGAGTACTTTTTTTTT128620.3527150171256825No Hit
GGTATCAACGCAGAGTACTTTTTTT120030.32915863400400924No Hit
GTACTTTTTTTTTTTTTTTTTTTTT104540.2866803598998511No Hit
GAGTACTTTTTTTTTTTTTTTTTTT51900.1423255278247778No Hit
ACGCAGAGTACTTTTTTTTTTTTTT51660.14166737509495225No Hit
GGTATCAACGCAGAGTACATGGGGG37380.10250728767033129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35150.012.89189151
CGTCTAG604.0943758E-412.66666619
GTGGTAT9500.012.11
TCGAATA550.003069807512.09090918
ATAACGC2050.012.04877953
GGTACGA1501.7644197E-1011.4000012
GTATAAC2800.011.1964281
GTACGAC1803.6379788E-1211.0833333
CGGTCTA700.001492237110.85714310
GGGTACG1855.456968E-1210.7837841
CGGCAAT2350.010.51063816
CACGATT1553.6689016E-910.41935514
TGGTATC11100.010.270272
TTAGACT2053.6379788E-1210.1951224
CAACGCT750.002649033410.1333344
TAGACTG1701.5625119E-910.0588245
GTCTAAC856.59748E-410.0588241
TATCACG2650.010.0377362
CGCACGA1752.577508E-99.77142812
ACGATTT1856.493792E-109.75675715
Produced by FastQC (version 0.11.5)