##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295364_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3646570 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51913853292272 33.0 32.0 34.0 32.0 34.0 2 32.69970246012006 33.0 32.0 34.0 32.0 34.0 3 32.82790732112643 33.0 32.0 34.0 32.0 34.0 4 32.92604200659798 33.0 32.0 34.0 32.0 34.0 5 32.67359271863697 33.0 32.0 34.0 32.0 34.0 6 35.51689587749584 37.0 35.0 37.0 33.0 37.0 7 35.46758817189853 37.0 35.0 37.0 33.0 37.0 8 35.402878869732376 37.0 35.0 37.0 33.0 37.0 9 35.37516707481277 37.0 34.0 37.0 33.0 37.0 10 35.253206986291225 37.0 34.0 37.0 33.0 37.0 11 35.18947366977735 37.0 34.0 37.0 33.0 37.0 12 35.15441140578681 37.0 34.0 37.0 33.0 37.0 13 36.87000249549577 38.0 38.0 38.0 36.0 38.0 14 36.8757851899182 38.0 38.0 38.0 36.0 38.0 15 36.74659200289587 38.0 38.0 38.0 35.0 38.0 16 36.88169019105625 38.0 38.0 38.0 36.0 38.0 17 36.87476532741727 38.0 38.0 38.0 36.0 38.0 18 36.946013651184536 38.0 38.0 38.0 36.0 38.0 19 36.9096120463888 38.0 38.0 38.0 36.0 38.0 20 36.91319651069361 38.0 38.0 38.0 36.0 38.0 21 36.88856843554354 38.0 38.0 38.0 36.0 38.0 22 36.92015729850243 38.0 38.0 38.0 36.0 38.0 23 37.471881795769725 39.0 38.0 39.0 36.0 39.0 24 37.43933340097681 39.0 38.0 39.0 36.0 39.0 25 37.319999616077574 39.0 38.0 39.0 35.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04878263791864157 1101 2 0.31417454098609454 1101 3 0.31821492218024616 1101 4 0.16946171605664517 1101 5 0.32510921336598386 1101 6 0.0969714917441209 1101 7 0.1351518886983527 1101 8 0.21377116695804688 1101 9 0.07720163976206607 1101 10 0.20431184735620178 1101 11 0.19643715620600233 1101 12 0.12636242076973048 1101 13 0.19425781681661647 1101 14 0.21825558817153734 1101 15 0.44723827433068664 1101 16 0.20151809895627082 1101 17 0.3137355963500639 1101 18 0.15447915997525996 1101 19 0.26108021427666017 1101 20 0.22560300817379897 1101 21 0.3187924858647051 1101 22 0.22392745422543925 1101 23 0.2807235061668294 1101 24 0.12541213664457018 1101 25 0.2771009655103356 1102 1 -0.03565790302501881 1102 2 0.033679301066783296 1102 3 0.1882470568445953 1102 4 0.1904249351534304 1102 5 0.30574605377076836 1102 6 0.1626382588237476 1102 7 0.25531167900197715 1102 8 0.2541630724356665 1102 9 0.1516583681870003 1102 10 0.09792609608854974 1102 11 0.09190245612523995 1102 12 0.19625249522841415 1102 13 0.1932495304820847 1102 14 0.2414297838410775 1102 15 0.3944079280534396 1102 16 0.031409664063268394 1102 17 0.17026732543903478 1102 18 0.008551341555055103 1102 19 0.12695210318125305 1102 20 0.15926345056943347 1102 21 0.2771074007782701 1102 22 0.2963461341630804 1102 23 0.21359543957766647 1102 24 0.2204963662694368 1102 25 0.12072237432933974 1103 1 0.1830272645109403 1103 2 0.12682380277510674 1103 3 0.15840930907205575 1103 4 0.1296804059881822 1103 5 0.2565056450253351 1103 6 0.18175218233491108 1103 7 0.18136251153021732 1103 8 0.17051698363474088 1103 9 0.134571376216563 1103 10 0.08029422182130475 1103 11 0.001350170524652583 1103 12 0.14326466549995587 1103 13 0.1572349840714793 1103 14 0.10349030137231097 1103 15 0.17187673422910876 1103 16 -5.960402754681127E-4 1103 17 0.1576536048851196 1103 18 0.1628262303638195 1103 19 0.1700103633599781 1103 20 0.1686455365328996 1103 21 0.1395875476691728 1103 22 0.3061841088541968 1103 23 0.13512124126334157 1103 24 0.01857007119339471 1103 25 -0.03519727749549162 1104 1 0.1295097678110153 1104 2 0.22704893092161882 1104 3 0.10846281911698696 1104 4 0.133671160194325 1104 5 0.11942452363025069 1104 6 0.17157156614744196 1104 7 0.1358163525037881 1104 8 0.10563491890729892 1104 9 0.17273247325015717 1104 10 0.20982230890593456 1104 11 0.23617585415934883 1104 12 0.21821066778982612 1104 13 0.29406225443167955 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-0.017103812694656995 2110 10 -0.0052035687003026965 2110 11 0.07889649427993817 2110 12 -0.0725807370012248 2110 13 -0.30608597552789973 2110 14 -0.21718770599228776 2110 15 -0.07566720229745982 2110 16 -0.16802944228070515 2110 17 -0.20374069757787794 2110 18 -0.09553857569006396 2110 19 -0.0393317971726006 2110 20 -0.08895973935468504 2110 21 -0.21214039176480526 2110 22 -0.5962434658604252 2110 23 -0.20361410250705347 2110 24 -0.053350914413421435 2110 25 -0.07285965335006495 2111 1 0.032384707871813134 2111 2 -0.06327147020996193 2111 3 -0.18492535185945513 2111 4 -0.11361345923253907 2111 5 -0.20921143262930286 2111 6 -0.061846704946020736 2111 7 -0.032754511190539404 2111 8 -0.3211520958470402 2111 9 -0.12379205682036343 2111 10 -0.30721254263893627 2111 11 -0.1066569708802163 2111 12 0.06951214370126024 2111 13 -0.8221051424471355 2111 14 -0.20964955102234484 2111 15 0.07204191418816208 2111 16 -0.1526617399333432 2111 17 0.008669762960273886 2111 18 -0.3060564607208036 2111 19 0.011420869122098054 2111 20 0.07483419395283875 2111 21 0.004569070828104316 2111 22 -0.03139576148459611 2111 23 0.023384402366986023 2111 24 0.039403966490503706 2111 25 0.13212139032087578 2112 1 -0.13111992312671106 2112 2 0.003376226574502539 2112 3 -0.13451315277168163 2112 4 -0.1738074128253544 2112 5 -0.4987439821360127 2112 6 -0.10087742622800988 2112 7 -0.06177649500787652 2112 8 -0.17259231163238553 2112 9 -0.1784665230960485 2112 10 -0.0889327571637466 2112 11 -0.24139339512916536 2112 12 -0.15588333303639956 2112 13 0.03378985978060456 2112 14 -0.30751305180308464 2112 15 0.015600892321991466 2112 16 -0.44643982577326824 2112 17 -0.3776996932161225 2112 18 -0.6481904710321444 2112 19 -0.11712991383087967 2112 20 -0.14596277799131485 2112 21 -0.11927442894259599 2112 22 -0.17225658158655932 2112 23 -0.12724545555342104 2112 24 -0.28963134325157824 2112 25 -0.117423569088551 2113 1 -0.09873238799988826 2113 2 -0.2415362016925826 2113 3 -0.22247183034236429 2113 4 -0.2621039138923109 2113 5 -0.34702473633231534 2113 6 -0.07076788033666048 2113 7 -0.304283637954164 2113 8 -0.16217469491228798 2113 9 -0.16274813031218116 2113 10 -0.1619941285967812 2113 11 -0.38472700260947335 2113 12 -0.12293747341894345 2113 13 -0.0019735371530558155 2113 14 -0.005038257693662729 2113 15 -0.08032069171999723 2113 16 -0.16017105420460354 2113 17 -0.15249937543851644 2113 18 -0.2113968472300911 2113 19 -0.07023641547574755 2113 20 -0.3702700044068692 2113 21 -0.10950262073419736 2113 22 -0.03379770855642761 2113 23 0.059414239983453854 2113 24 -0.3259348894654863 2113 25 0.04809882506154395 2114 1 -0.7043231833327646 2114 2 -0.3001712756192063 2114 3 -0.3322952445640368 2114 4 -0.29249006447336967 2114 5 -0.40699959769222716 2114 6 -0.075637705251836 2114 7 -0.18599160647888624 2114 8 -0.25760562005433485 2114 9 -0.24141464035761828 2114 10 -0.18647808261166432 2114 11 -0.33862465715990453 2114 12 -0.17430574167754997 2114 13 -0.12978438718216267 2114 14 -0.16795149186151548 2114 15 -0.2744401238002183 2114 16 -0.2935467358682189 2114 17 -0.2685660802349972 2114 18 -0.40853228683931775 2114 19 -0.2718077086683621 2114 20 -0.6050952249002037 2114 21 -0.237401104071985 2114 22 -0.1329751907116261 2114 23 -0.2663974285780526 2114 24 -0.4149790711032324 2114 25 -0.07438562119150305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 6.0 17 5.0 18 3.0 19 9.0 20 12.0 21 86.0 22 237.0 23 797.0 24 2038.0 25 4820.0 26 9820.0 27 17581.0 28 28413.0 29 42474.0 30 59003.0 31 79413.0 32 104106.0 33 142470.0 34 225411.0 35 525926.0 36 1927996.0 37 475943.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.179672952939335 18.447033787915768 13.234080245271585 25.139213013873313 2 17.729811850588362 20.245820044589845 36.632232481482596 25.39213562333919 3 18.77281938917942 24.47883901858459 29.78259021491429 26.965751377321702 4 14.520988216323833 17.344161773940993 35.40096583913102 32.733884170604156 5 14.869617201918514 35.273668131970595 33.38984305799696 16.466871608113927 6 31.52831290774618 35.17960713766636 18.12415502787551 15.167924926711951 7 28.2886932103319 30.778923755748554 22.20080788247586 18.731575151443685 8 26.612295938374963 32.59026976035014 20.781967712123997 20.0154665891509 9 26.365269280447105 16.011567034226683 20.48097253035044 37.14219115497578 10 17.347178307286022 26.888610392780066 31.538240044754385 24.225971255179523 11 33.34654209298053 22.70188149411639 23.98267412938734 19.968902283515742 12 24.032776005945315 24.551016434622124 29.01710374406634 22.39910381536622 13 28.434446616957853 21.15681311479006 25.778197045442703 24.63054322280938 14 23.329567237157107 20.58984196107575 26.57875757218427 29.501833229582868 15 24.502313132615033 27.441897454320085 23.784625003770667 24.27116440929421 16 23.991778575483263 26.74173264190733 24.89166531836767 24.37482346424174 17 23.060684423992956 26.46297753779579 26.399410953306806 24.076927084904444 18 22.973204956986976 26.15847220812983 27.650422177553153 23.21790065733004 19 24.015362381635345 25.4105364767439 27.702745319574284 22.87135582204647 20 24.283861272373766 25.35758260502335 27.33055995085793 23.027996171744956 21 24.55161974129113 24.943659384023892 27.164074733242472 23.340646141442505 22 24.2416572285737 25.038186569845088 27.010341224767387 23.709814976813828 23 24.334429340448697 24.950597410717467 26.942057879047983 23.772915369785856 24 23.701258991326096 25.236153426370535 27.20257666793727 23.860010914366104 25 24.155932835513923 24.736176735946383 27.33187625631758 23.776014172222116 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1526.0 1 1526.0 2 1939.5 3 2353.0 4 2353.0 5 2353.0 6 7352.0 7 12351.0 8 12351.0 9 12351.0 10 11590.0 11 10829.0 12 10829.0 13 10829.0 14 12656.5 15 14484.0 16 14484.0 17 14484.0 18 25045.5 19 35607.0 20 35607.0 21 35607.0 22 55977.0 23 76347.0 24 76347.0 25 76347.0 26 106518.5 27 136690.0 28 136690.0 29 136690.0 30 166156.0 31 195622.0 32 195622.0 33 195622.0 34 228966.5 35 262311.0 36 262311.0 37 262311.0 38 295944.0 39 329577.0 40 329577.0 41 329577.0 42 369449.0 43 409321.0 44 409321.0 45 409321.0 46 422925.0 47 436529.0 48 436529.0 49 436529.0 50 445730.0 51 454931.0 52 454931.0 53 454931.0 54 440268.5 55 425606.0 56 425606.0 57 425606.0 58 381753.5 59 337901.0 60 337901.0 61 337901.0 62 288604.0 63 239307.0 64 239307.0 65 239307.0 66 189397.0 67 139487.0 68 139487.0 69 139487.0 70 106646.0 71 73805.0 72 73805.0 73 73805.0 74 53445.5 75 33086.0 76 33086.0 77 33086.0 78 22775.0 79 12464.0 80 12464.0 81 12464.0 82 8557.0 83 4650.0 84 4650.0 85 4650.0 86 2941.0 87 1232.0 88 1232.0 89 1232.0 90 833.5 91 435.0 92 435.0 93 435.0 94 269.0 95 103.0 96 103.0 97 103.0 98 59.5 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3646570.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.95902262178994 #Duplication Level Percentage of deduplicated Percentage of total 1 72.63654098785052 26.119390205506892 2 12.049260902363866 8.665592907279025 3 4.538686290149812 4.896201689421148 4 2.4536277877916035 3.529202285066027 5 1.5460025756284796 2.7796370795185004 6 1.035737253223012 2.2346459591326124 7 0.7849441153614479 1.9758076240786242 8 0.6020669116897556 1.73197901578265 9 0.480199686218056 1.5540760241722356 >10 3.3497010815981754 23.59048067978015 >50 0.3409505832444174 8.47377164875425 >100 0.1770198366529152 11.026791886824183 >500 0.003736774249613349 0.8785927451682166 >1k 0.0010676497847949102 0.791882477202978 >5k 1.5252139782784432E-4 0.28398846604266514 >10k+ 3.0504279565568863E-4 1.4679593062697862 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 18212 0.499428229815964 No Hit TATCAACGCAGAGTACTTTTTTTTT 12862 0.3527150171256825 No Hit GGTATCAACGCAGAGTACTTTTTTT 12003 0.32915863400400924 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 10454 0.2866803598998511 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 5190 0.1423255278247778 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 5166 0.14166737509495225 No Hit GGTATCAACGCAGAGTACATGGGGG 3738 0.10250728767033129 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.742303040939842E-5 0.0 6 0.0 0.0 0.0 2.742303040939842E-5 0.0 7 0.0 0.0 0.0 2.742303040939842E-5 0.0 8 0.0 0.0 0.0 5.484606081879684E-5 0.0 9 0.0 0.0 0.0 5.484606081879684E-5 0.0 10 0.0 0.0 0.0 1.0969212163759368E-4 0.0 11 0.0 2.742303040939842E-5 0.0 1.9196121286578895E-4 0.0 12 0.0 2.742303040939842E-5 0.0 3.0165333450338265E-4 0.0 13 0.0 2.742303040939842E-5 0.0 4.387684865503747E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3515 0.0 12.8918915 1 CGTCTAG 60 4.0943758E-4 12.666666 19 GTGGTAT 950 0.0 12.1 1 TCGAATA 55 0.0030698075 12.090909 18 ATAACGC 205 0.0 12.0487795 3 GGTACGA 150 1.7644197E-10 11.400001 2 GTATAAC 280 0.0 11.196428 1 GTACGAC 180 3.6379788E-12 11.083333 3 CGGTCTA 70 0.0014922371 10.857143 10 GGGTACG 185 5.456968E-12 10.783784 1 CGGCAAT 235 0.0 10.510638 16 CACGATT 155 3.6689016E-9 10.419355 14 TGGTATC 1110 0.0 10.27027 2 TTAGACT 205 3.6379788E-12 10.195122 4 CAACGCT 75 0.0026490334 10.133334 4 TAGACTG 170 1.5625119E-9 10.058824 5 GTCTAAC 85 6.59748E-4 10.058824 1 TATCACG 265 0.0 10.037736 2 CGCACGA 175 2.577508E-9 9.771428 12 ACGATTT 185 6.493792E-10 9.756757 15 >>END_MODULE