FastQCFastQC Report
Wed 25 May 2016
SRR1295363_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295363_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27553297
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN1501510.5449474884983819No Hit
GTATCAACGCAGAGTACTTTTTTTT479330.17396466201485797No Hit
TATCAACGCAGAGTACTTTTTTTTT339070.12305968320234055No Hit
GGTATCAACGCAGAGTACTTTTTTT297230.10787456760619246No Hit
GTACTTTTTTTTTTTTTTTTTTTTT275750.10007876734316043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTT22800.012.66350615
TATAACG13750.012.0986132
CGCACGA24100.011.94172312
ATAACGC16500.011.9251863
CGATTTC26850.011.45952916
GGTATCA174300.011.3430661
CACGATT26000.011.17822414
TATCACG17050.010.6490092
TGTCACG24200.010.2051097
ACGCACG24300.010.08449311
GTCACGC24700.010.0368378
GTATTAA44050.010.01236251
GTATAAC21900.09.8524951
GCACGAT30600.09.74668113
TAACGCA24050.09.5252424
GTATCAA333100.09.3340571
TCGTACC6800.09.0866692
GTATCAC25200.09.0526391
CTTGCGA10200.08.93946811
GCGAATT9350.08.93914114
Produced by FastQC (version 0.11.5)