FastQCFastQC Report
Wed 25 May 2016
SRR1295363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295363_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27553297
Sequences flagged as poor quality0
Sequence length25
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGNACTTTTTTTT613620.2227029309777338No Hit
TATCAACGCAGAGTNCTTTTTTTTT455260.16522886535139517No Hit
GTACTTTTTTTTTTNTTTTTTTTTT421860.1531069040485427No Hit
GGTATCAACGCAGANTACTTTTTTT378460.13735561301429733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTT15100.015.80278315
CGCACGA16300.014.92457112
CACGATT17050.013.81366814
ACGCACG16600.012.22793211
TATAACG18450.011.3004822
CGAATTA4400.011.26904615
ATAACGC21100.011.24029353
GCACGAT22950.010.4649913
GGTATCA208550.010.2337311
CGATTTC25200.010.14963716
CACGCAC21450.010.11324610
TATCACG18300.09.9954412
ACGAAAC15950.09.81184215
CGCTCGA4900.09.80291914
GTATAAC23500.09.7977551
CGGTCTA2402.0070911E-79.68433610
TAACGCA26150.09.3659984
GACGTAG10500.09.29696310
TGTCACG21850.09.293637
CATCGGA9150.09.14455315
Produced by FastQC (version 0.11.5)