FastQCFastQC Report
Wed 25 May 2016
SRR1295362_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295362_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3957998
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACATGGGGG45870.11589192313892023No Hit
GTATCAACGCAGAGTACATGGGGGC45810.11574033134933367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGCG5600.013.40178611
AGCGCGA5600.013.23214312
GCGCGAG5900.012.72033913
GGTATCA19550.012.5370851
CGCGAGG6050.012.40495914
GCTACGA5650.012.10619452
AAACGCG550.003069887612.09090911
ATAACGC1353.7289283E-1011.9629623
ACGTAAT1459.822543E-1111.79310315
CGCGGAA6450.011.78294614
ACGAGCG6200.011.4919365
TATAACG1251.8348146E-811.4000012
CGAGCGT6000.011.46
GCAAGCG3000.011.08333313
CCGGCCT5150.011.0679619
TAGCCCG2150.011.0465125
CGTAATT1553.1286618E-1011.03225816
TCGCGGA7000.010.99285713
GTTCGCG6800.010.89705911
GGCTACG6900.010.8768121
Produced by FastQC (version 0.11.5)