##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295362_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3957998 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.33986752898814 32.0 32.0 33.0 29.0 34.0 2 31.62962967641722 33.0 32.0 33.0 29.0 34.0 3 31.867335203302275 33.0 32.0 33.0 30.0 34.0 4 31.957358492854215 33.0 32.0 34.0 31.0 34.0 5 31.590462147782794 33.0 32.0 33.0 30.0 34.0 6 34.816767466784974 37.0 34.0 37.0 32.0 37.0 7 34.78286623692079 37.0 34.0 37.0 32.0 37.0 8 34.77011281966287 37.0 34.0 37.0 32.0 37.0 9 34.756905637648124 37.0 34.0 37.0 32.0 37.0 10 34.718074390133594 37.0 34.0 37.0 32.0 37.0 11 34.69800843759901 37.0 33.0 37.0 32.0 37.0 12 34.65942049490677 37.0 33.0 37.0 32.0 37.0 13 36.37949261217413 38.0 38.0 38.0 34.0 38.0 14 36.395934510325674 38.0 38.0 38.0 34.0 38.0 15 36.41959015643767 38.0 38.0 38.0 34.0 38.0 16 36.36661463699578 38.0 38.0 38.0 34.0 38.0 17 36.39189964219285 38.0 38.0 38.0 34.0 38.0 18 36.27269720702234 38.0 37.0 38.0 33.0 38.0 19 36.35633848223269 38.0 38.0 38.0 34.0 38.0 20 36.37271570122067 38.0 38.0 38.0 34.0 38.0 21 36.379719999858516 38.0 38.0 38.0 34.0 38.0 22 36.3616750185321 38.0 38.0 38.0 34.0 38.0 23 36.86061286539306 39.0 38.0 39.0 34.0 39.0 24 36.86513686970029 39.0 38.0 39.0 34.0 39.0 25 36.71176640311592 38.0 37.0 39.0 33.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07456392669745782 1101 2 -0.11833451746912971 1101 3 -0.04415679955992857 1101 4 -0.04263407211839265 1101 5 0.046810446390264104 1101 6 -0.034517627702499 1101 7 0.14515298644565888 1101 8 0.04032509151161179 1101 9 0.02579575409851742 1101 10 0.05993109997284307 1101 11 -0.08396300406941748 1101 12 -0.003909318431844611 1101 13 -0.06546927336862751 1101 14 -0.08833132946946876 1101 15 -0.08221588617391973 1101 16 0.06208810126430819 1101 17 -0.07603908745984 1101 18 0.035211582480904724 1101 19 -0.03208268303577455 1101 20 0.07027894597366213 1101 21 0.021593467946878775 1101 22 -0.013788641228295262 1101 23 0.11973700185664171 1101 24 0.003046358674033911 1101 25 -0.026697192111583945 1102 1 0.07723927331674219 1102 2 -0.010092581665308131 1102 3 0.1199145340357255 1102 4 -0.045994382082440666 1102 5 0.06651850130457859 1102 6 0.025376828431866727 1102 7 0.16674841366766913 1102 8 0.1903945648847909 1102 9 0.13879105720700835 1102 10 0.07367894493633997 1102 11 0.07068442115233609 1102 12 0.1167885210987265 1102 13 0.18353785546103296 1102 14 0.09094404385554355 1102 15 0.09794365536399141 1102 16 0.2163071246223467 1102 17 0.08105992031294562 1102 18 0.2078209464198295 1102 19 0.11110314558038681 1102 20 0.17062934758097725 1102 21 0.1250094099024892 1102 22 0.15519863299064696 1102 23 0.09174627898715215 1102 24 0.1887907348540665 1102 25 0.13893735159506093 1103 1 -0.2741199231137408 1103 2 -0.11614484304253381 1103 3 -5.942414351594039E-4 1103 4 -0.19130974244404086 1103 5 -0.16965328935632584 1103 6 -0.09404422597024364 1103 7 0.03898923986778158 1103 8 0.06381022464300656 1103 9 0.14715803060994403 1103 10 0.07585428003730499 1103 11 0.06314392309581507 1103 12 -0.020970193972942752 1103 13 0.07367643725685724 1103 14 0.043408958792035435 1103 15 -0.03803568351689535 1103 16 0.16664045183929943 1103 17 -0.011158810477809311 1103 18 0.09522413182860134 1103 19 -0.03634202875163339 1103 20 0.07967400018609538 1103 21 0.013596358778762863 1103 22 -0.026359651488128577 1103 23 -0.14409611422856017 1103 24 -0.03707289747832476 1103 25 -0.06392688625858511 1104 1 0.02945743147602542 1104 2 -0.004687844548545428 1104 3 -0.019645098257210947 1104 4 0.031419701401365074 1104 5 -0.03443892789594116 1104 6 0.04560101494691082 1104 7 0.11521387606372713 1104 8 0.1937083684180152 1104 9 0.18164834847622302 1104 10 0.018725859209801854 1104 11 0.08023001522089146 1104 12 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2110 14 0.2292458602199403 2110 15 0.10803169459330775 2110 16 0.22211806577004722 2110 17 -0.2674418821508766 2110 18 0.18878826342200483 2110 19 0.20907108046090883 2110 20 0.22471698283886354 2110 21 0.2667285073817425 2110 22 0.37846524468481135 2110 23 0.11310474986983365 2110 24 0.11645755357233867 2110 25 0.34182643332257356 2111 1 0.370779290032047 2111 2 0.3596132527977609 2111 3 0.23897494262824637 2111 4 0.11616992836144746 2111 5 0.2572502714191778 2111 6 0.2802977482935205 2111 7 0.33176557650863714 2111 8 0.3035481690035624 2111 9 0.10794610231638302 2111 10 0.27440905644071023 2111 11 0.2092728831404287 2111 12 0.07038016332210617 2111 13 0.09706038166061148 2111 14 -0.003590882148337471 2111 15 0.0831946721286414 2111 16 0.08584285619242848 2111 17 -0.4308614671662667 2111 18 -0.10275068569466583 2111 19 0.2746053185854933 2111 20 0.3557948108796154 2111 21 0.334831250569124 2111 22 0.36628276087689216 2111 23 0.2796447521161909 2111 24 0.2938128486193179 2111 25 0.4891571816618381 2112 1 0.10976314411688648 2112 2 0.04186372643842873 2112 3 0.1440427199555998 2112 4 0.08497432484162815 2112 5 0.10912094461929556 2112 6 -0.026933652568658317 2112 7 0.12303260381245451 2112 8 0.28819738397015016 2112 9 0.24856401400445804 2112 10 0.3521471962435285 2112 11 0.2705557408503907 2112 12 0.11086663447231615 2112 13 0.1575834262105289 2112 14 -0.07560557206698348 2112 15 -0.05634155979289801 2112 16 0.22316403273071472 2112 17 0.24955987852708716 2112 18 -0.010070020686143266 2112 19 0.28655239388805853 2112 20 0.2970989007945093 2112 21 0.08501203788048883 2112 22 0.23267175159502074 2112 23 0.1747513667928331 2112 24 0.08862940620477389 2112 25 0.02597734217058445 2113 1 0.03814387802140473 2113 2 0.04460433593940394 2113 3 0.14491389735258764 2113 4 0.15714083137619284 2113 5 -0.047709350611413015 2113 6 0.0511667399753577 2113 7 0.11573245308260027 2113 8 0.09606677493928828 2113 9 0.23185463320150745 2113 10 0.44998772670682996 2113 11 0.2745526160707499 2113 12 0.16278065381744966 2113 13 0.2594116500076282 2113 14 0.05234897539088479 2113 15 0.07193674098513725 2113 16 0.2516671795468781 2113 17 0.23637028211874878 2113 18 0.14878781873514413 2113 19 0.2093881864978897 2113 20 0.08318702046167203 2113 21 0.11717104465011374 2113 22 0.23666330132117963 2113 23 0.024098828478656742 2113 24 0.1615381764489996 2113 25 -0.2135497980169916 2114 1 -0.01663413632351407 2114 2 0.01049691013565024 2114 3 -0.0813465174865513 2114 4 0.015527164737662247 2114 5 0.061608289155149265 2114 6 -0.059784192353426135 2114 7 -0.021381081698073956 2114 8 0.104870424256994 2114 9 0.23026895118471202 2114 10 0.3503365793366484 2114 11 -0.06908656850982453 2114 12 0.14913741798720537 2114 13 0.1773933607997762 2114 14 0.13547623773227713 2114 15 0.07539997498923867 2114 16 0.145357909029741 2114 17 0.2374074903073975 2114 18 0.11115553158902713 2114 19 0.061370078249765925 2114 20 0.02907918294695122 2114 21 0.13817463115603346 2114 22 0.19786061197275728 2114 23 0.17545001110288183 2114 24 0.1813843603366294 2114 25 -0.02078925945767196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 2.0 15 42.0 16 287.0 17 1176.0 18 2829.0 19 4878.0 20 7642.0 21 10829.0 22 14531.0 23 18678.0 24 23272.0 25 28341.0 26 34308.0 27 41304.0 28 49244.0 29 58738.0 30 70811.0 31 87546.0 32 113553.0 33 161829.0 34 277552.0 35 708134.0 36 2043889.0 37 198583.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.01166903065641 18.034774145919226 13.534266566077093 25.419290257347278 2 17.457714733559744 19.61612916428962 37.2486039659444 25.677552136206234 3 18.15458219028913 23.766661832572932 30.135992994438098 27.942762982699836 4 14.10771304078476 17.09313647960408 36.337891024704916 32.46125945490624 5 15.229517549023521 34.94966394626778 33.22566105389644 16.595157450812252 6 31.353982493169525 35.16088183975838 18.565421205366956 14.919714461705135 7 29.171742886176293 30.516033610931586 22.00807074687759 18.30415275601453 8 26.757163596343403 32.55282089581652 20.80988924198547 19.880126265854607 9 26.545213009203138 16.516152863139393 20.20177877805901 36.73685534959846 10 17.580352491335265 26.390134608456094 31.463911805918045 24.5656010942906 11 33.1407443864297 22.595463666227218 24.317394804140882 19.946397143202194 12 24.321462517161454 24.08538357017866 29.04210664078153 22.551047271878357 13 27.60953896389033 20.967898417331185 25.853727061004072 25.56883555777441 14 23.20339727306583 20.675300998130876 26.3446065409836 29.7766951878197 15 24.624267116860597 27.29053930800369 23.264615090760532 24.82057848437518 16 24.298167912161652 26.84493524251402 24.206278022373937 24.650618822950392 17 23.83967854455712 26.299482718283336 25.86262044599315 23.998218291166392 18 23.547055860058546 26.68467745562277 26.24910371354407 23.51916297077462 19 24.50989111161754 26.212620622850242 25.86830513810265 23.409183127429575 20 24.996829205067815 25.956506294343757 25.625909866553748 23.420754634034683 21 25.022574543999266 25.154434135641303 25.683464216000107 24.139527104359328 22 24.782528945188957 25.5873802867005 25.57350963795333 24.056581130157216 23 24.87401459020444 25.577653146868695 25.4340451915337 24.114287071393164 24 24.242685317172974 25.80461132117803 25.648269655517765 24.30443370613123 25 24.44442872381441 25.13308495860786 25.856177794935725 24.566308522642004 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 727.0 1 727.0 2 742.5 3 758.0 4 758.0 5 758.0 6 1434.5 7 2111.0 8 2111.0 9 2111.0 10 4148.5 11 6186.0 12 6186.0 13 6186.0 14 11844.5 15 17503.0 16 17503.0 17 17503.0 18 29440.0 19 41377.0 20 41377.0 21 41377.0 22 62600.0 23 83823.0 24 83823.0 25 83823.0 26 112132.5 27 140442.0 28 140442.0 29 140442.0 30 172569.0 31 204696.0 32 204696.0 33 204696.0 34 237233.5 35 269771.0 36 269771.0 37 269771.0 38 313814.5 39 357858.0 40 357858.0 41 357858.0 42 394875.5 43 431893.0 44 431893.0 45 431893.0 46 454974.5 47 478056.0 48 478056.0 49 478056.0 50 492814.0 51 507572.0 52 507572.0 53 507572.0 54 485219.0 55 462866.0 56 462866.0 57 462866.0 58 425376.0 59 387886.0 60 387886.0 61 387886.0 62 325329.5 63 262773.0 64 262773.0 65 262773.0 66 210937.0 67 159101.0 68 159101.0 69 159101.0 70 122805.5 71 86510.0 72 86510.0 73 86510.0 74 61779.5 75 37049.0 76 37049.0 77 37049.0 78 25161.5 79 13274.0 80 13274.0 81 13274.0 82 8851.5 83 4429.0 84 4429.0 85 4429.0 86 2687.0 87 945.0 88 945.0 89 945.0 90 614.5 91 284.0 92 284.0 93 284.0 94 173.0 95 62.0 96 62.0 97 62.0 98 54.0 99 46.0 100 46.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3957998.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.86672433712994 #Duplication Level Percentage of deduplicated Percentage of total 1 75.28078667373929 27.00075223509292 2 11.064255360946754 7.936771940533788 3 4.152867895930125 4.468493040955279 4 2.160205442995963 3.099179725420155 5 1.3781684853251768 2.4715194576639017 6 0.9543010425581218 2.0536591456840716 7 0.7003148346287403 1.758259938598224 8 0.5218407450492877 1.4973374520452247 9 0.40572859595644817 1.309694013617463 >10 2.857438405527044 19.874226940307903 >50 0.2968792223998497 7.428884008362628 >100 0.20707169625378102 14.102478280662591 >500 0.015000561264087178 3.7492233046484906 >1k 0.005141037425400894 3.2495205164073178 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACATGGGGG 4587 0.11589192313892023 No Hit GTATCAACGCAGAGTACATGGGGGC 4581 0.11574033134933367 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.52652982644256E-5 0.0 10 0.0 0.0 0.0 7.57958947932768E-5 0.0 11 0.0 0.0 0.0 1.010611930577024E-4 0.0 12 0.0 0.0 0.0 1.010611930577024E-4 0.0 13 0.0 0.0 0.0 1.26326491322128E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCGCG 560 0.0 13.401786 11 AGCGCGA 560 0.0 13.232143 12 GCGCGAG 590 0.0 12.720339 13 GGTATCA 1955 0.0 12.537085 1 CGCGAGG 605 0.0 12.404959 14 GCTACGA 565 0.0 12.1061945 2 AAACGCG 55 0.0030698876 12.090909 11 ATAACGC 135 3.7289283E-10 11.962962 3 ACGTAAT 145 9.822543E-11 11.793103 15 CGCGGAA 645 0.0 11.782946 14 ACGAGCG 620 0.0 11.491936 5 TATAACG 125 1.8348146E-8 11.400001 2 CGAGCGT 600 0.0 11.4 6 GCAAGCG 300 0.0 11.083333 13 CCGGCCT 515 0.0 11.067961 9 TAGCCCG 215 0.0 11.046512 5 CGTAATT 155 3.1286618E-10 11.032258 16 TCGCGGA 700 0.0 10.992857 13 GTTCGCG 680 0.0 10.897059 11 GGCTACG 690 0.0 10.876812 1 >>END_MODULE