FastQCFastQC Report
Wed 25 May 2016
SRR1295362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295362_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3957998
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT172370.43549794618390414No Hit
TATCAACGCAGAGTACTTTTTTTTT112320.28377983010602836No Hit
GGTATCAACGCAGAGTACTTTTTTT112090.28319872824594655No Hit
GTACTTTTTTTTTTTTTTTTTTTTT94200.23799910965088916No Hit
GGTATCAACGCAGAGTACATGGGGG49650.1254422058828731No Hit
ACGCAGAGTACTTTTTTTTTTTTTT47980.12122290107271404No Hit
GAGTACTTTTTTTTTTTTTTTTTTT47590.12023755444040143No Hit
GTATCAACGCAGAGTACATGGGGGC45340.11455286233090567No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA350.00217099816.28571510
CGTTAAT902.2919266E-914.7777782
AAGCGCG5800.014.41379311
CGCGAGG5850.014.29059914
AGCGCGA5850.014.29059912
GCGCGAG6050.013.81818213
GGTATCA31950.013.7073551
ATAACGC2450.013.1836733
TAGCCCG2200.012.9545465
TATAACG2350.012.936172
GCTACGA3750.012.162
GTGGTAT9450.012.0634921
AGGGCTA7100.011.77464818
CGAGCGT3700.011.5540546
ACGAGCG3800.011.55
GCGAGGG7350.011.37414915
TATCACG2300.011.1521742
GAAGCGC7850.011.13375810
GGCTACG4500.010.7666661
AGCCCGG4950.010.7474756
Produced by FastQC (version 0.11.5)