##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295362_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3957998 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.142345701033705 33.0 32.0 33.0 32.0 34.0 2 32.365436263484725 33.0 32.0 34.0 32.0 34.0 3 32.481354209880855 33.0 32.0 34.0 32.0 34.0 4 32.56878502717788 33.0 32.0 34.0 32.0 34.0 5 32.265220699959926 33.0 32.0 34.0 32.0 34.0 6 35.21916130326493 37.0 34.0 37.0 32.0 37.0 7 35.156878805901364 37.0 34.0 37.0 32.0 37.0 8 35.087864622468224 37.0 34.0 37.0 32.0 37.0 9 35.08356093156186 37.0 34.0 37.0 32.0 37.0 10 34.95222786873566 37.0 34.0 37.0 32.0 37.0 11 34.9004468925957 37.0 34.0 37.0 32.0 37.0 12 34.85726546602601 37.0 34.0 37.0 32.0 37.0 13 36.54270365977951 38.0 37.0 38.0 34.0 38.0 14 36.5524616232752 38.0 37.0 38.0 34.0 38.0 15 36.39531803704802 38.0 37.0 38.0 34.0 38.0 16 36.56071604887117 38.0 37.0 38.0 34.0 38.0 17 36.550774659310086 38.0 37.0 38.0 34.0 38.0 18 36.634223665600636 38.0 37.0 38.0 34.0 38.0 19 36.59342223012745 38.0 37.0 38.0 34.0 38.0 20 36.59768549655659 38.0 37.0 38.0 34.0 38.0 21 36.57234945545703 38.0 37.0 38.0 34.0 38.0 22 36.61173274973863 38.0 37.0 38.0 34.0 38.0 23 37.10237018816078 38.0 37.0 39.0 34.0 39.0 24 37.060068751929634 38.0 37.0 39.0 34.0 39.0 25 36.91889990848909 38.0 37.0 39.0 34.0 39.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3976474496572209 1101 2 0.5832806031592384 1101 3 0.5794586152764154 1101 4 0.4880334865096998 1101 5 0.6721307985293876 1101 6 0.4109282751289314 1101 7 0.43413573489682733 1101 8 0.46907280574162513 1101 9 0.3231313433772556 1101 10 0.4899409046238645 1101 11 0.48052279664948827 1101 12 0.4687545037167098 1101 13 0.5354568595922089 1101 14 0.5242362082092953 1101 15 0.8263909646734291 1101 16 0.5256459867230561 1101 17 0.5946933725900934 1101 18 0.42782636372847804 1101 19 0.5196895786786087 1101 20 0.522008877181726 1101 21 0.5883742698420846 1101 22 0.5415085164373892 1101 23 0.5892773166260099 1101 24 0.4805292430728656 1101 25 0.6383180891283047 1102 1 0.2358177611223553 1102 2 0.27202565023918623 1102 3 0.4090685503388727 1102 4 0.48095569231964674 1102 5 0.6562521579869625 1102 6 0.35279880980299794 1102 7 0.5365321147611866 1102 8 0.4558899852203737 1102 9 0.4123964341101072 1102 10 0.29769865113442506 1102 11 0.40451237579154764 1102 12 0.45334809854704616 1102 13 0.4807879469208203 1102 14 0.5416540103873828 1102 15 0.7274517400335796 1102 16 0.3346609895964363 1102 17 0.5026620336296048 1102 18 0.26087156686814694 1102 19 0.42828529871616183 1102 20 0.45084419819666977 1102 21 0.5823159489215826 1102 22 0.5842522498443898 1102 23 0.5592374253200845 1102 24 0.5870362246065355 1102 25 0.5702764477388129 1103 1 0.4789585203481437 1103 2 0.4012729689727266 1103 3 0.4253745495865573 1103 4 0.41002106414945416 1103 5 0.5912625405357161 1103 6 0.5207587842365413 1103 7 0.46345612796032754 1103 8 0.41226179667695817 1103 9 0.38944609296921584 1103 10 0.2852560762623355 1103 11 0.22502001973282404 1103 12 0.4216881751852455 1103 13 0.4371036253339824 1103 14 0.3826519277612519 1103 15 0.5186798013563632 1103 16 0.30971287175832884 1103 17 0.4744897444840319 1103 18 0.4348444743793607 1103 19 0.4710969357336978 1103 20 0.47892143885466965 1103 21 0.40067584261895917 1103 22 0.5631632749184732 1103 23 0.4718735376289871 1103 24 0.39403652430897296 1103 25 0.364693083059052 1104 1 0.38482545673033286 1104 2 0.45728257536035244 1104 3 0.33175477718130963 1104 4 0.41467561738113545 1104 5 0.4252448248598384 1104 6 0.4076961466682576 1104 7 0.38566960720143584 1104 8 0.3673715394075643 1104 9 0.4635828993354565 1104 10 0.469306353849511 1104 11 0.42400609136189615 1104 12 0.4299633919527537 1104 13 0.5357233531827035 1104 14 0.27522613666008766 1104 15 0.5634483301888977 1104 16 0.24946994956294333 1104 17 0.44571977201997015 1104 18 0.4105954746734355 1104 19 0.44974330838667953 1104 20 0.5139467915195937 1104 21 0.2261226109339205 1104 22 0.4714089952638716 1104 23 0.44948798107338206 1104 24 0.3850314715986016 1104 25 0.4729023561638428 1105 1 0.3611386716776508 1105 2 0.3644605956388034 1105 3 0.3476992500172287 1105 4 0.4180821867816604 1105 5 0.4306503910415529 1105 6 0.4714328569691446 1105 7 0.5203384109530234 1105 8 0.4123362986256396 1105 9 0.4083769458112698 1105 10 0.2582746681617607 1105 11 0.42916363230540355 1105 12 0.34785289814917064 1105 13 0.49528743949591814 1105 14 5.062323553914894E-4 1105 15 0.44656504596356683 1105 16 0.34763456398376746 1105 17 0.2672756302440433 1105 18 0.2508773212337587 1105 19 0.22721840317655762 1105 20 0.3565538914556754 1105 21 0.2872078152097828 1105 22 0.39523412660557966 1105 23 0.2606507824986082 1105 24 0.3833843916440429 1105 25 0.3471590838109506 1106 1 0.5368302005177199 1106 2 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0.41764047744246113 1107 15 -0.15197624424720857 1107 16 0.35695812275920247 1107 17 0.3988471262327167 1107 18 0.27673369460440966 1107 19 0.12247855261755802 1107 20 0.28827470178364223 1107 21 0.15440271544628104 1107 22 0.12128987096110677 1107 23 0.24204603379848777 1107 24 0.473665748158588 1107 25 0.5948077509998058 1108 1 0.06684447480730427 1108 2 0.1425681721194323 1108 3 0.19279065845848464 1108 4 0.22734857347208504 1108 5 0.3343015636963713 1108 6 0.17268938348939145 1108 7 0.25061022334468674 1108 8 0.20726777170327892 1108 9 0.10170692233803891 1108 10 0.22165430360141158 1108 11 0.2121942886818573 1108 12 0.08161314191853819 1108 13 0.2723984114431133 1108 14 0.311436899669836 1108 15 0.05337907790503493 1108 16 0.34301384141257074 1108 17 0.20385640844302344 1108 18 0.28277469511617426 1108 19 0.2809138662868875 1108 20 0.3333345757057984 1108 21 0.2286681584085244 1108 22 0.12621986725251588 1108 23 0.3057712059869715 1108 24 0.4980399008059777 1108 25 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-0.10548174157909074 2110 20 -0.12606487044450887 2110 21 -0.3518920410304176 2110 22 -0.8368953562762727 2110 23 -0.35229477239468565 2110 24 -0.09902469747848386 2110 25 -0.15319976911826672 2111 1 -0.16991013371288588 2111 2 -0.2490547331262718 2111 3 -0.4285334471851243 2111 4 -0.3938824161143941 2111 5 -0.43569618054636194 2111 6 -0.2642366894371264 2111 7 -0.25895268276461536 2111 8 -0.6857990505020908 2111 9 -0.39117899171530723 2111 10 -0.5577618346367785 2111 11 -0.4669541851163359 2111 12 -0.18188864374010194 2111 13 -1.2930743250747554 2111 14 -0.5356144990723024 2111 15 -0.12157365328120306 2111 16 -0.3612754776151519 2111 17 -0.24180074944518992 2111 18 -0.6492388610813151 2111 19 -0.2183631255729921 2111 20 -0.18796694252710466 2111 21 -0.2079228224584213 2111 22 -0.311155193867549 2111 23 -0.20558380591820224 2111 24 -0.25264164308851633 2111 25 -0.23081057629414659 2112 1 -0.44583467318071257 2112 2 -0.3182769850890992 2112 3 -0.5384111583482571 2112 4 -0.5428117759306552 2112 5 -0.9824120689897882 2112 6 -0.42726460401090804 2112 7 -0.4047374855747137 2112 8 -0.5887239688393464 2112 9 -0.5869761487291001 2112 10 -0.5565799689052611 2112 11 -0.5727135769511662 2112 12 -0.4933221979513007 2112 13 -0.4409594772076417 2112 14 -0.8722906601034452 2112 15 -0.4728361796248066 2112 16 -1.1325726054271215 2112 17 -0.8925744038391201 2112 18 -1.2181070005112957 2112 19 -0.6074431193921157 2112 20 -0.5333205981753082 2112 21 -0.5305491612974294 2112 22 -0.5364888651231041 2112 23 -0.5739220184346436 2112 24 -0.8449815842236674 2112 25 -0.5838286603053149 2113 1 -0.5466029399931145 2113 2 -0.6581570035410174 2113 3 -0.6513467103192028 2113 4 -0.8140498468236324 2113 5 -0.9235653580150078 2113 6 -0.5363825111871705 2113 7 -0.9618269670992916 2113 8 -0.808780918572559 2113 9 -0.8098693832212618 2113 10 -0.6780166623683499 2113 11 -0.9504644701650875 2113 12 -0.7042446396573325 2113 13 -0.5451757260617427 2113 14 -0.5901222878095282 2113 15 -0.6399169493754115 2113 16 -0.7194330686643156 2113 17 -0.7056552499830175 2113 18 -0.7997141391710088 2113 19 -0.5647451432268227 2113 20 -0.9430167438396992 2113 21 -0.5460393712027027 2113 22 -0.43550721126700864 2113 23 -0.5179092049283085 2113 24 -1.0760123575001188 2113 25 -0.5885490681867509 2114 1 -1.3773103056332765 2114 2 -0.7862996443558217 2114 3 -0.8124751490367501 2114 4 -0.8351881703972808 2114 5 -1.0890185635124325 2114 6 -0.6936038766552031 2114 7 -0.8137365939222647 2114 8 -0.8363824404962727 2114 9 -0.9194463767574632 2114 10 -0.8885605111720807 2114 11 -1.0108117459488142 2114 12 -0.7781972128301646 2114 13 -0.8435959179949108 2114 14 -0.8748756325990499 2114 15 -1.1081442505845303 2114 16 -1.0076983355550837 2114 17 -0.8687125580112323 2114 18 -1.1392047237481648 2114 19 -0.9191911802639865 2114 20 -1.4244252525605248 2114 21 -0.8234252534751576 2114 22 -0.7660029112666749 2114 23 -0.962864018098422 2114 24 -1.244522353111293 2114 25 -0.8122941755846966 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 3.0 16 5.0 17 11.0 18 4.0 19 10.0 20 34.0 21 108.0 22 374.0 23 1188.0 24 3178.0 25 7103.0 26 14052.0 27 25071.0 28 40367.0 29 59170.0 30 82247.0 31 110401.0 32 147740.0 33 206135.0 34 325672.0 35 708572.0 36 1913928.0 37 312625.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.149086482610656 18.640358080019244 13.9230995063666 25.2874559310035 2 17.55377339756109 20.317645435899664 36.69731515781463 25.431266008724613 3 18.115269386189684 24.491775892761947 30.30014163726207 27.0928130837863 4 14.496065940407247 17.832904412786466 35.867754354600486 31.803275292205807 5 14.973175832832657 35.36416642959395 33.197667103419455 16.464990634153935 6 31.041880263709075 35.797542090723645 18.202434665201952 14.958142980365327 7 28.699862910491618 31.049308261398817 21.96297218947559 18.287856638633976 8 26.81456635400018 32.539784001912075 20.739474855722513 19.90617478836523 9 26.59061475018431 16.342605529361055 20.369363501446944 36.69741621900769 10 17.479973461330705 26.727300013794853 31.390794032740793 24.40193249213365 11 33.16371054255207 22.69652485928492 24.247182540264042 19.89258205789897 12 24.407617184243144 24.397738452621756 29.011889344057273 22.182755019077828 13 27.825178284577202 21.033891376397865 26.11135730740642 25.02957303161851 14 23.267495334762675 20.67327977426972 26.785890240470057 29.273334650497553 15 24.647561721860395 27.303601467206402 23.790613335327606 24.258223475605597 16 23.95069426513101 26.847209119357817 24.764085277455926 24.438011338055247 17 23.492280693421268 26.193418996169278 26.39359595431832 23.920704356091136 18 23.212265392756642 26.20205972817571 27.390160379060326 23.195514500007327 19 24.216712590557147 25.71567747128725 27.18167113778228 22.88593880037332 20 24.67550514174085 25.51299924861003 26.9097912631588 22.90170434649032 21 24.73727879599737 24.675303019354736 27.03546085672605 23.551957327921844 22 24.420098241585773 25.17047760003921 26.894480492410555 23.51494366596446 23 24.527122045033877 25.081821668429345 26.813581007367866 23.577475279168915 24 23.90205856597199 25.319037553834033 26.95883120709005 23.82007267310393 25 24.271841471370124 24.594176146627664 27.20248974355217 23.931492638450045 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1241.0 1 1241.0 2 1660.0 3 2079.0 4 2079.0 5 2079.0 6 6517.5 7 10956.0 8 10956.0 9 10956.0 10 10987.0 11 11018.0 12 11018.0 13 11018.0 14 14132.5 15 17247.0 16 17247.0 17 17247.0 18 29628.0 19 42009.0 20 42009.0 21 42009.0 22 64994.5 23 87980.0 24 87980.0 25 87980.0 26 121170.5 27 154361.0 28 154361.0 29 154361.0 30 187778.0 31 221195.0 32 221195.0 33 221195.0 34 249423.0 35 277651.0 36 277651.0 37 277651.0 38 317281.5 39 356912.0 40 356912.0 41 356912.0 42 391866.0 43 426820.0 44 426820.0 45 426820.0 46 449807.0 47 472794.0 48 472794.0 49 472794.0 50 487555.5 51 502317.0 52 502317.0 53 502317.0 54 478723.5 55 455130.0 56 455130.0 57 455130.0 58 416791.0 59 378452.0 60 378452.0 61 378452.0 62 315464.0 63 252476.0 64 252476.0 65 252476.0 66 202597.5 67 152719.0 68 152719.0 69 152719.0 70 117483.5 71 82248.0 72 82248.0 73 82248.0 74 58454.5 75 34661.0 76 34661.0 77 34661.0 78 23558.5 79 12456.0 80 12456.0 81 12456.0 82 8311.5 83 4167.0 84 4167.0 85 4167.0 86 2493.5 87 820.0 88 820.0 89 820.0 90 524.0 91 228.0 92 228.0 93 228.0 94 136.5 95 45.0 96 45.0 97 45.0 98 30.5 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3957998.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.39091326946404 #Duplication Level Percentage of deduplicated Percentage of total 1 73.17680734755649 24.434404274785383 2 11.909833762438824 7.953604524306589 3 4.4854037584159245 4.493151836273825 4 2.3992194949796275 3.204485202850883 5 1.5288487078803812 2.552482730348299 6 1.0443753262465656 2.092358756368038 7 0.7590727594824496 1.774229287396385 8 0.5711671563881447 1.525743438505836 9 0.465028259830658 1.3974946462669764 >10 3.0872064235947674 20.03983408827655 >50 0.3257257295955048 7.584724079025479 >100 0.2248616024808065 14.318484381836935 >500 0.0157979167087571 3.6424727732870927 >1k 0.006349401928325736 3.7473179168724133 >5k 7.5588118194354E-5 0.23775668334975508 >10k+ 2.26764354583062E-4 1.0014553802496373 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 17237 0.43549794618390414 No Hit TATCAACGCAGAGTACTTTTTTTTT 11232 0.28377983010602836 No Hit GGTATCAACGCAGAGTACTTTTTTT 11209 0.28319872824594655 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 9420 0.23799910965088916 No Hit GGTATCAACGCAGAGTACATGGGGG 4965 0.1254422058828731 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4798 0.12122290107271404 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 4759 0.12023755444040143 No Hit GTATCAACGCAGAGTACATGGGGGC 4534 0.11455286233090567 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.52652982644256E-5 0.0 10 0.0 0.0 0.0 1.26326491322128E-4 0.0 11 0.0 0.0 0.0 1.26326491322128E-4 0.0 12 0.0 0.0 0.0 1.26326491322128E-4 0.0 13 0.0 0.0 0.0 1.515917895865536E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTCTA 35 0.002170998 16.285715 10 CGTTAAT 90 2.2919266E-9 14.777778 2 AAGCGCG 580 0.0 14.413793 11 CGCGAGG 585 0.0 14.290599 14 AGCGCGA 585 0.0 14.290599 12 GCGCGAG 605 0.0 13.818182 13 GGTATCA 3195 0.0 13.707355 1 ATAACGC 245 0.0 13.183673 3 TAGCCCG 220 0.0 12.954546 5 TATAACG 235 0.0 12.93617 2 GCTACGA 375 0.0 12.16 2 GTGGTAT 945 0.0 12.063492 1 AGGGCTA 710 0.0 11.774648 18 CGAGCGT 370 0.0 11.554054 6 ACGAGCG 380 0.0 11.5 5 GCGAGGG 735 0.0 11.374149 15 TATCACG 230 0.0 11.152174 2 GAAGCGC 785 0.0 11.133758 10 GGCTACG 450 0.0 10.766666 1 AGCCCGG 495 0.0 10.747475 6 >>END_MODULE