FastQCFastQC Report
Wed 25 May 2016
SRR1295361_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295361_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10506728
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN563660.5364752946873661No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA382050.3636241463565061No Hit
GTACATGGGAAGCAGTGGTATCAAC297540.28318997122605627No Hit
GTATCAACGCAGAGTACTTTTTTTT296460.2821620584448365No Hit
TATCAACGCAGAGTACTTTTTTTTT210210.20007180161131038No Hit
GGTATCAACGCAGAGTACTTTTTTT193640.18430095458833615No Hit
GTACTTTTTTTTTTTTTTTTTTTTT168940.16079220857340173No Hit
CCCATGTACTCTGCGTTGATACCAC162850.15499592261263448No Hit
GGTATCAACGCAGAGTACATGGGGG140850.1340569585507496No Hit
GAGTACATGGGAAGCAGTGGTATCA123410.11745807067623716No Hit
GCTTCCCATGTACTCTGCGTTGATA107970.10276272498916884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC13900.014.3597963
ACGATTT8900.014.30047915
CACGATT9050.013.958837514
TATAACG13350.013.955442
GTATAAC14050.013.394881
TGCGCGT3600.013.19165110
AGCGCGA5000.013.10766412
ACGCACG7800.012.90783211
CGCACGA9750.012.85924612
CGCGGAA5100.012.8506514
GGCTACG9150.012.673271
TAACGCA16200.012.5557454
CGCGTGG3950.012.26363812
TGTCACG8150.012.2379037
AAGCGCG5450.012.02526411
CGCTCGA3950.012.02317414
GCTACGC6800.012.0214892
GTCACGC8500.011.8452878
AACGAGC4900.011.8325263
TACGCCC7700.011.7267234