FastQCFastQC Report
Wed 25 May 2016
SRR1295361_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295361_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10506728
Sequences flagged as poor quality0
Sequence length25
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGNACTTTTTTTT413030.3931100148400149No Hit
GTACTTTTTTTTTTNTTTTTTTTTT317940.30260610153798595No Hit
TATCAACGCAGAGTNCTTTTTTTTT315940.3007025593505419No Hit
GGTATCAACGCAGANTACTTTTTTT269000.25602642421122923No Hit
GTACATGGGAAGCANTGGTATCAAC156240.1487047156831318No Hit
GAGTACTTTTTTTTNTTTTTTTTTT118550.11283246316074805No Hit
ACGCAGAGTACTTTNTTTTTTTTTT112130.10672209273905253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA856.493792E-1021.8608210
CACGATT5450.021.59320414
ACGATTT5800.020.29016515
AGCGCGA2950.019.42136412
AAGCGCG3200.017.9042411
ACGCACG5400.017.4920311
TGCGCGT2150.017.285310
CGCACGA6900.017.05550212
GTTCGCG2700.016.63176511
CGCGTGG2500.016.10398512
CGCTCGA1950.015.8816414
CACGCAC6100.015.73859610
GCGCGAG3800.015.07711113
CGCGGAA3000.014.96844814
CTTGCGA2800.014.37869411
AAACGCG1104.9808023E-614.07704311
TCGCGGA3200.014.03291913
CGCTGTA2550.013.96650212
GCGAATT2950.013.64744514
CGCGAGG4250.013.48071114
Produced by FastQC (version 0.11.5)