FastQCFastQC Report
Wed 25 May 2016
SRR1295360_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295360_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38523142
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN2057350.5340556074060625No Hit
GTACATGGGAAGCAGTGGTATCAAC1012620.2628601789542504No Hit
CCCATGTACTCTGCGTTGATACCAC948820.2462987053340561No Hit
GTATCAACGCAGAGTACTTTTTTTT849770.22058688774659138No Hit
TATCAACGCAGAGTACTTTTTTTTT493810.12818528665185203No Hit
GGTATCAACGCAGAGTACTTTTTTT487690.12659663118859826No Hit
GAGTACATGGGAAGCAGTGGTATCA439340.1140457338604416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC11900.09.3450741
GTCCTAA28050.08.810171
TACGACC15550.08.5574494
GTATTAG38350.08.5506191
GTACGAC14900.08.4203983
GTATTAA72050.08.3109451
CTAGACA35750.08.2419954
TAGACAG48550.08.20360855
GTCTTAG30950.08.04608251
GTTCTAG34400.08.0404131
TCGTACC10650.08.03259852
GTCTAGG34350.08.02444651
CTAGGAC31200.08.0120953
TAGACTG33600.07.9496355
CTACACT54100.07.92366174
GGTACGA13600.07.89773652
GTCCTAG33600.07.8923941
ACGTCGC11600.07.86104156
GTCTTAA40900.07.80835961
CGTACCC12450.07.78710133
Produced by FastQC (version 0.11.5)