FastQCFastQC Report
Wed 25 May 2016
SRR1295360_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295360_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38523142
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGNACTTTTTTTT1220180.31673948090734655No Hit
TATCAACGCAGAGTNCTTTTTTTTT715380.18570136361151435No Hit
GGTATCAACGCAGANTACTTTTTTT707840.18374409854730955No Hit
CCCATGTACTCTGCNTTGATACCAC525010.13628431450373388No Hit
GTACATGGGAAGCANTGGTATCAAC500150.12983105064483058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGTT212200.09.76878111
TGCGTTG213700.09.64946312
CTGTCGC21800.09.0962839
GTCGTAC9100.08.8602411
CGCACGA8000.08.71469412
CGTGCGA2155.1569143E-58.64713910
CGTTGAT237550.08.64152614
TCTGCGT242100.08.61350510
CTCTGCG251100.08.3969569
GTCCTAG30300.08.3667871
GCGTTGA249550.08.23840213
TACGACC12550.08.1524144
GAGTACT492400.07.916312
GTCTTAG27500.07.8654771
GTATTAG33400.07.8455571
GTTGATA265400.07.76390815
TGTCGCC25200.07.684915510
CGACGTA7300.07.63994269
AGTACTT506850.07.6264213
CCGATCG3906.441951E-87.54743679
Produced by FastQC (version 0.11.5)