FastQCFastQC Report
Wed 25 May 2016
SRR1295359_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295359_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11074598
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC903410.8157497003502971No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN591580.5341774030985142No Hit
CCCATGTACTCTGCGTTGATACCAC556560.5025554877928752No Hit
GCTTCCCATGTACTCTGCGTTGATA392380.3543063143240053No Hit
GAGTACATGGGAAGCAGTGGTATCA374680.33832379288169195No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA335450.30290038518779644No Hit
GCGTTGATACCACTGCTTCCCATGT224210.2024543012757664No Hit
GTATCAACGCAGAGTACATGGGAAG215150.19427341741885348No Hit
GTATCAACGCAGAGTACTTTTTTTT197390.1782367179377527No Hit
CATGTACTCTGCGTTGATACCACTG175070.1580824875088017No Hit
TATCAACGCAGAGTACATGGGAAGC157990.1426598058006259No Hit
ACGCAGAGTACATGGGAAGCAGTGG151030.1363751533012756No Hit
TATCAACGCAGAGTACTTTTTTTTT147950.13359401397685045No Hit
GGTATCAACGCAGAGTACATGGGGG137630.1242753913054No Hit
GTGGTATCAACGCAGAGTACATGGG125570.11338560550911193No Hit
GGGAAGCAGTGGTATCAACGCAGAG123090.11114624657256181No Hit
GGTATCAACGCAGAGTACATGGGAA116420.10512345459401777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG13150.015.9748172
ACGATTT15100.015.53644515
CACGATT15850.015.46079914
ATAACGC14850.015.1690943
CGCACGA15750.015.01668612
TGTCGCG4500.014.9872827
TCGCGCA4850.014.4923039
TGTCACG11900.014.2883847
CGATTTC17200.014.02367816
GTCACGC12400.013.9414838
ACGCACG12650.013.59062511
TAACGCA17400.013.54777054
GTATAAC16950.013.5147131
TATCACG8850.012.9960532
TGCGCGT4550.012.94284410
CGCGTGG4700.012.7320412
CGTCGTA3500.012.48358210
TTTCCCG19050.012.41559619
TACGCCC10050.012.2954014
CGACTCG3950.012.0330535
Produced by FastQC (version 0.11.5)