FastQCFastQC Report
Wed 25 May 2016
SRR1295359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295359_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11074598
Sequences flagged as poor quality0
Sequence length25
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCANTGGTATCAAC474260.42824127792268396No Hit
GTATCAACGCAGAGNACTTTTTTTT385270.34788621672768616No Hit
GTACTTTTTTTTTTNTTTTTTTTTT324900.2933740800343272No Hit
CCCATGTACTCTGCNTTGATACCAC301230.27200084373265737No Hit
TATCAACGCAGAGTNCTTTTTTTTT280890.253634488583694No Hit
GGTATCAACGCAGANTACTTTTTTT258680.23357958455918673No Hit
GCTTCCCATGTACTNTGCGTTGATA202150.1825348423482279No Hit
GAGTACATGGGAAGNAGTGGTATCA191560.17297241850223366No Hit
GTATCAACGCAGAGNACATGGGAAG118440.10694744856653036No Hit
GAGTACTTTTTTTTNTTTTTTTTTT113250.10226104821141138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTT9550.023.81721915
TCGCGCA2800.022.1034229
CGCACGA10100.021.90744812
CACGATT10450.021.17370614
ACGCACG8150.020.31436511
CGCGCAC3400.018.20363610
CGTCGTA2350.017.77759410
CGCGTGG2800.017.68353512
TGCGCGT3000.017.02040110
CACGCAC10900.015.47309310
GCACGAT15600.014.3820413
GCGCGTG3650.013.9893711
CGCTCGA2005.456968E-1213.92578214
ATAACGC20550.013.3947663
CATCGGA2900.013.33887315
TGTCGCG4600.013.1476927
TATAACG20350.012.9930322
CGATTTC17200.012.89289116
GTATAAC21750.012.6588311
CTGCGCG4700.012.5095959
Produced by FastQC (version 0.11.5)