FastQCFastQC Report
Wed 25 May 2016
SRR1295358_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295358_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4213529
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC725061.7207903398789945No Hit
CCCATGTACTCTGCGTTGATACCAC460221.092243580143865No Hit
GAGTACATGGGAAGCAGTGGTATCA311480.7392378217878647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA302400.7176881896386615No Hit
GCTTCCCATGTACTCTGCGTTGATA257280.6106045549941629No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN228570.5424668965135876No Hit
GTATCAACGCAGAGTACATGGGAAG198760.4717185997770515No Hit
GCGTTGATACCACTGCTTCCCATGT178220.4229708636157482No Hit
CATGTACTCTGCGTTGATACCACTG148080.3514393754024239No Hit
ACGCAGAGTACATGGGAAGCAGTGG147020.34892366944667996No Hit
GTATCAACGCAGAGTACTTTTTTTT144940.34398718983540877No Hit
TATCAACGCAGAGTACATGGGAAGC143900.34151895002977317No Hit
GGTATCAACGCAGAGTACATGGGAA116580.27668018898173No Hit
TATCAACGCAGAGTACTTTTTTTTT104310.24755970589024068No Hit
GGTATCAACGCAGAGTACTTTTTTT102820.2440234777071666No Hit
GTGGTATCAACGCAGAGTACATGGG100560.23865980274492No Hit
GGGAAGCAGTGGTATCAACGCAGAG94330.22387409698616054No Hit
ACTCTGCGTTGATACCACTGCTTCC92640.21986320730200265No Hit
CTGCTTCCCATGTACTCTGCGTTGA79760.18929500663220783No Hit
CATGGGAAGCAGTGGTATCAACGCA75950.180252705036562No Hit
CAGTGGTATCAACGCAGAGTACATG74670.17721487142962586No Hit
GCAGTGGTATCAACGCAGAGTACAT66960.15891667056284647No Hit
CCACTGCTTCCCATGTACTCTGCGT65800.15616363385656062No Hit
ATGGGAAGCAGTGGTATCAACGCAG64910.15405139017673783No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA58280.13831636141581083No Hit
ACATGGGAAGCAGTGGTATCAACGC58110.13791289913988963No Hit
GTACTCTGCGTTGATACCACTGCTT57010.13530226088392888No Hit
GCAGAGTACATGGGAAGCAGTGGTA56400.13385454330562338No Hit
ATACCACTGCTTCCCATGTACTCTG54510.12936899212038175No Hit
GATACCACTGCTTCCCATGTACTCT53070.1259514293125786No Hit
GGTATCAACGCAGAGTACATGGGGG51120.12132347967701183No Hit
GTACTTTTTTTTTTTTTTTTTTTTT50790.12054028820022361No Hit
AAGCAGTGGTATCAACGCAGAGTAC45910.10895854757377961No Hit
TACCACTGCTTCCCATGTACTCTGC44690.10606311241716859No Hit
GGAAGCAGTGGTATCAACGCAGAGT43040.1021471550332275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG7600.016.8820692
CGCACGA5950.016.12432712
TGTCACG5100.016.0182787
ATAACGC8750.015.5311043
GTCACGC5450.015.5124738
ACGCACG5250.015.37930911
GTATAAC8700.015.2932121
ACGATTT6350.015.25693715
TATCACG3650.015.1022152
CGGCAAT3350.014.74187616
CACGCAC5800.014.57602910
TAACGCA9650.014.4770464
TTTCCCG6850.014.14413519
TGTCGCG1152.5465852E-1114.0423147
GTCGGCA3450.014.04097114
CGATTTC7250.014.01651316
CGACTCG1700.013.9774435
TACGCCC4900.013.9645314
CACGATT6950.013.93995714
GCTACGC4950.013.631972
Produced by FastQC (version 0.11.5)