##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295358_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4213529
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.031343322901066	33.0	33.0	33.0	27.0	33.0
2	30.853035543365195	33.0	33.0	33.0	27.0	33.0
3	31.004593062015235	33.0	33.0	33.0	27.0	33.0
4	34.439821346904225	37.0	37.0	37.0	27.0	37.0
5	34.74443465323248	37.0	37.0	37.0	33.0	37.0
6	34.69776878241493	37.0	37.0	37.0	33.0	37.0
7	34.617696709812606	37.0	37.0	37.0	33.0	37.0
8	34.60964645075423	37.0	37.0	37.0	27.0	37.0
9	34.45725874913879	37.0	37.0	37.0	27.0	37.0
10	34.56911035856167	37.0	37.0	37.0	27.0	37.0
11	34.53363392063992	37.0	37.0	37.0	27.0	37.0
12	34.52185899278253	37.0	37.0	37.0	27.0	37.0
13	34.37300846867317	37.0	37.0	37.0	27.0	37.0
14	36.32233954008623	40.0	37.0	40.0	27.0	40.0
15	36.340902839401366	40.0	37.0	40.0	27.0	40.0
16	36.293464456990804	40.0	37.0	40.0	27.0	40.0
17	36.336037084353755	40.0	37.0	40.0	27.0	40.0
18	36.33839899998315	40.0	37.0	40.0	27.0	40.0
19	36.34744652285531	40.0	37.0	40.0	27.0	40.0
20	36.37674856397096	40.0	37.0	40.0	27.0	40.0
21	36.36077478047499	40.0	37.0	40.0	27.0	40.0
22	36.39518156870405	40.0	37.0	40.0	27.0	40.0
23	36.39876312706048	40.0	37.0	40.0	27.0	40.0
24	36.43673272451667	40.0	37.0	40.0	27.0	40.0
25	36.40647471513783	40.0	37.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.34229595988302464
1101	2	0.3252383774651406
1101	3	0.3905749102688034
1101	4	0.36530157915016304
1101	5	0.2765165690499387
1101	6	0.2637230383668623
1101	7	0.2810501107989083
1101	8	0.31629545600418396
1101	9	0.3320743511470994
1101	10	0.355502298527945
1101	11	0.2906429635436041
1101	12	0.2717841756910033
1101	13	0.36012967875922897
1101	14	0.529424680617538
1101	15	0.48310487780090483
1101	16	0.534814409111597
1101	17	0.39786474490056634
1101	18	0.4940866680252185
1101	19	0.5208221108165603
1101	20	0.5091650326017145
1101	21	0.4728402377594989
1101	22	0.5625375127371015
1101	23	0.5139860240982728
1101	24	0.5619703545736741
1101	25	0.5283876618524062
1102	1	0.31739507527693434
1102	2	0.28219336986964905
1102	3	0.35902851112578915
1102	4	0.34248022373876097
1102	5	0.294803766812727
1102	6	0.35284651447367565
1102	7	0.3616240450327055
1102	8	0.3210025980667055
1102	9	0.4547955242475581
1102	10	0.3343202791249169
1102	11	0.3601034583629428
1102	12	0.36422714238209153
1102	13	0.3885360451610751
1102	14	0.5028563485373354
1102	15	0.5287699459301507
1102	16	0.5430752839931543
1102	17	0.5464445704344527
1102	18	0.46222660726130016
1102	19	0.50109739219495
1102	20	0.4806637825106037
1102	21	0.44991674602055554
1102	22	0.4819210217001597
1102	23	0.5239510891586363
1102	24	0.4868656288744475
1102	25	0.44562247183088743
1103	1	0.07684723996975507
1103	2	0.04466482611035261
1103	3	0.13329630848055274
1103	4	0.09570773325858539
1103	5	0.06558717972965411
1103	6	0.1844679303745096
1103	7	0.13369157063027615
1103	8	0.14187501943771963
1103	9	0.12908738234574457
1103	10	0.190007560234136
1103	11	0.10840311344572484
1103	12	0.17152683669308288
1103	13	0.27083582386509875
1103	14	0.3512917076098816
1103	15	0.3909668978402081
1103	16	0.40106044176214084
1103	17	0.2753157863238087
1103	18	0.3746474218647151
1103	19	0.39349802697021374
1103	20	0.3050285620055888
1103	21	0.3779950864974211
1103	22	0.3170016787365526
1103	23	0.28924016671024333
1103	24	0.30619866320425615
1103	25	0.3190914222388699
1104	1	0.14844628531581705
1104	2	0.12939036310319096
1104	3	0.14915038280717852
1104	4	0.11002754232779921
1104	5	0.08979447601332424
1104	6	0.1140622024508815
1104	7	0.11954223547855491
1104	8	0.15960430685674964
1104	9	0.33570177034437876
1104	10	0.20722542590068826
1104	11	0.2853550398009972
1104	12	0.3005755922334856
1104	13	0.31106766839288724
1104	14	0.3079913325672621
1104	15	0.2683961511644526
1104	16	0.23143889385896443
1104	17	0.25690460323482256
1104	18	0.29077425445932903
1104	19	0.25490273205033276
1104	20	0.2158707031579965
1104	21	0.18621192932046426
1104	22	0.20738694979008443
1104	23	0.24900832218245483
1104	24	0.26375065774908535
1104	25	0.2618121859620146
1105	1	0.20445956196910586
1105	2	0.11926916997627046
1105	3	0.16767483264578686
1105	4	0.20398173734605507
1105	5	0.09720409528671325
1105	6	0.1291100401519074
1105	7	0.22134385387334987
1105	8	0.19385602275619362
1105	9	0.24782117484357968
1105	10	0.1444594082666555
1105	11	0.19593662152582425
1105	12	0.23169770308502535
1105	13	0.2612203719393733
1105	14	0.33147955139082086
1105	15	0.2736028067507945
1105	16	0.32679790642078643
1105	17	0.4124601881175778
1105	18	0.3330167846204759
1105	19	0.3258419727591644
1105	20	0.2505392223346732
1105	21	0.3409684499908465
1105	22	0.3286263244336851
1105	23	0.3514418588718726
1105	24	0.3334121760766706
1105	25	0.38908815047085454
1106	1	0.10687043884531278
1106	2	0.050607710667673445
1106	3	0.11542363178513071
1106	4	0.10246648323280283
1106	5	0.09991803843799829
1106	6	0.08815528204836909
1106	7	0.14247937669440347
1106	8	0.060821980890381155
1106	9	0.11112126503609687
1106	10	0.12672976075696596
1106	11	0.10544515758536477
1106	12	0.12429890918019026
1106	13	0.15658185087806942
1106	14	0.2003099830063988
1106	15	0.17666312712709953
1106	16	0.22488306905952982
1106	17	0.2148863463419488
1106	18	0.2859636979675031
1106	19	0.22101319367045136
1106	20	0.21517835376690897
1106	21	0.12697278803943135
1106	22	0.17704594661186945
1106	23	0.17513900252907177
1106	24	0.14767122467394955
1106	25	0.18380282937849302
1107	1	0.10533625773791044
1107	2	0.10136405633796741
1107	3	0.10272964155621267
1107	4	0.16168664909709918
1107	5	0.1466488295564332
1107	6	0.11149204567683313
1107	7	0.15526582749529894
1107	8	0.16675316444027288
1107	9	0.13993321312498352
1107	10	0.19600693184627715
1107	11	0.19138271134438156
1107	12	0.20278282788227386
1107	13	0.11998304584198394
1107	14	0.16350869801800627
1107	15	0.18389300601798908
1107	16	0.14276329062005289
1107	17	0.2237567874089308
1107	18	0.2132926939587989
1107	19	0.1923818956640133
1107	20	0.2482568550071349
1107	21	0.22351024639559114
1107	22	0.3102397111468278
1107	23	0.2635203153062591
1107	24	0.30088187255680765
1107	25	0.3370350698500104
1108	1	0.09264911832228861
1108	2	0.13306219345738413
1108	3	0.11882073476940747
1108	4	0.1313390871991018
1108	5	0.10825126883361236
1108	6	0.08701211912393347
1108	7	0.09177595313379072
1108	8	0.0950713120855653
1108	9	0.07464818884434266
1108	10	0.17357098761828382
1108	11	0.08873604362781862
1108	12	0.1073751304939563
1108	13	0.09869295245204768
1108	14	0.1343422423140055
1108	15	0.1386611091255645
1108	16	0.07875403394066893
1108	17	0.12125396258001331
1108	18	0.16389709293677157
1108	19	0.1289755262792056
1108	20	0.2049116891273215
1108	21	0.16514676481478574
1108	22	0.14721459659798342
1108	23	-0.0077265501509131695
1108	24	0.1774943132762985
1108	25	0.12415147889372946
1109	1	-0.027655132520965964
1109	2	-0.015524005391441165
1109	3	-0.06108973975537779
1109	4	-0.06594062297085657
1109	5	0.00917110724027026
1109	6	-0.1108282550296451
1109	7	-0.11070810063050374
1109	8	-0.06102244161754555
1109	9	-0.07866895757180714
1109	10	-0.10299020499424216
1109	11	-0.05982635486920174
1109	12	-0.007741748313847552
1109	13	-0.11680437046027947
1109	14	-0.03990163958965809
1109	15	-0.041437960591991896
1109	16	-0.04501582931074921
1109	17	-0.11829657832064555
1109	18	-0.012619069811840689
1109	19	-0.05022324743026019
1109	20	-0.04349047375224302
1109	21	-0.05621277555121651
1109	22	-0.17709612713757394
1109	23	-0.17382468633230985
1109	24	-0.11086130937103178
1109	25	-0.1145391362158179
1110	1	-0.12740612525094264
1110	2	-0.1647579762513658
1110	3	-0.09195162657216471
1110	4	-0.048159959311377065
1110	5	-0.01714092338739448
1110	6	-0.06477671854757006
1110	7	-0.053065935549405197
1110	8	-0.04758169125165068
1110	9	-0.08824760218047345
1110	10	-0.10813108792408599
1110	11	-0.0792826252636516
1110	12	-0.051060465622740026
1110	13	-0.08372093165563399
1110	14	-0.1899093999788306
1110	15	-0.1362585756347059
1110	16	-0.11154335882272193
1110	17	-0.09227003254109434
1110	18	-0.18489858478376675
1110	19	-0.08063235115405121
1110	20	-0.19583881625308663
1110	21	-0.17636365701145706
1110	22	-0.1543218217486384
1110	23	-0.09571140189567728
1110	24	-0.23222901610480307
1110	25	-0.18332973767222427
1111	1	-0.9944997277998127
1111	2	-1.562724069988871
1111	3	-1.297884512922689
1111	4	-1.6440506783873374
1111	5	-1.626171974881757
1111	6	-1.593168960333152
1111	7	-1.5524819996181733
1111	8	-1.5128025260963867
1111	9	-1.4501032086080556
1111	10	-1.49792589432397
1111	11	-1.420156926566655
1111	12	-1.4510389727460975
1111	13	-1.4329738846055164
1111	14	-1.764955375812555
1111	15	-1.730133868930352
1111	16	-1.7923005043116405
1111	17	-1.797370717028521
1111	18	-1.794439511933625
1111	19	-1.8329409610147849
1111	20	-1.8424959911251264
1111	21	-1.912159927033393
1111	22	-1.8923875628173121
1111	23	-2.003998695428386
1111	24	-2.0420659032007933
1111	25	-2.0961725079969185
1112	1	-0.6130426857324487
1112	2	-0.7006436375843101
1112	3	-0.6813492429109793
1112	4	-0.6839680920183611
1112	5	-0.92535207649955
1112	6	-0.6958207401271324
1112	7	-0.6185856525928557
1112	8	-0.6159708849539385
1112	9	-0.6044781512115946
1112	10	-0.6122380407279664
1112	11	-0.6712090969312996
1112	12	-0.7063929670907427
1112	13	-0.5315634338576345
1112	14	-0.8739894242007935
1112	15	-0.9041142439406329
1112	16	-0.9244516456694569
1112	17	-1.0035249870331668
1112	18	-0.9401053889528797
1112	19	-0.9878870120465919
1112	20	-0.9338528448056422
1112	21	-0.9813699877513287
1112	22	-1.0002371182841685
1112	23	-1.0281410756331937
1112	24	-0.9663413821500129
1112	25	-0.9795556074608314
1113	1	-0.3342324416249305
1113	2	-0.26875692840341614
1113	3	-0.3726232664319298
1113	4	-0.33172091478966337
1113	5	-0.3955409020671041
1113	6	-0.3692879919107739
1113	7	-0.38534477261984534
1113	8	-0.3866393624789879
1113	9	-0.4891402781448875
1113	10	-0.42788673660264465
1113	11	-0.42131547599964847
1113	12	-0.31615023023571354
1113	13	-0.4342585921405586
1113	14	-0.6042180516998101
1113	15	-0.6354312787670722
1113	16	-0.5459240659435594
1113	17	-0.6284248144570981
1113	18	-0.5882532921130093
1113	19	-0.5244702545258093
1113	20	-0.5667484344826903
1113	21	-0.5995767883252796
1113	22	-0.563681431374718
1113	23	-0.5763177526357808
1113	24	-0.5406590719968847
1113	25	-0.4930621696803641
1114	1	-0.26242359204673704
1114	2	-0.37163790500067506
1114	3	-0.2849391836418924
1114	4	-0.3593283323052887
1114	5	-0.3064822868350987
1114	6	-0.329967210384865
1114	7	-0.29912401696888224
1114	8	-0.2754704139273514
1114	9	-0.24846654241289912
1114	10	-0.28410148948745473
1114	11	-0.226360395739583
1114	12	-0.3710832935142747
1114	13	-0.364099753598083
1114	14	-0.505740629226203
1114	15	-0.45935214404920544
1114	16	-0.5649580309311872
1114	17	-0.5422864617054586
1114	18	-0.5304601163277596
1114	19	-0.5426507172304227
1114	20	-0.4977603799636583
1114	21	-0.5015681568731054
1114	22	-0.4980372340342001
1114	23	-0.5709844286665628
1114	24	-0.528968907845254
1114	25	-0.5368529287347599
1115	1	-0.37885475546726965
1115	2	-0.37323013430095386
1115	3	-0.3732872340299771
1115	4	-0.4121922302461698
1115	5	-0.38139082267747426
1115	6	-0.44275067779254584
1115	7	-0.38639674175004757
1115	8	-0.406622767869699
1115	9	-0.46317375896104807
1115	10	-0.482161010837558
1115	11	-0.5217049032642294
1115	12	-0.4269642808879226
1115	13	-0.5182164055006595
1115	14	-0.6972693086127251
1115	15	-0.6455687134560719
1115	16	-0.6048679478747445
1115	17	-0.6974828651090732
1115	18	-0.6661165860553524
1115	19	-0.6159203204043848
1115	20	-0.5584293144164931
1115	21	-0.661272009371352
1115	22	-0.5896123167910119
1115	23	-0.5217660045322816
1115	24	-0.6589176408062087
1115	25	-0.6518180286151534
1116	1	-0.235607312890739
1116	2	-0.23516886375987767
1116	3	-0.1904934021362017
1116	4	-0.31599582919370306
1116	5	-0.28382129086283925
1116	6	-0.3332218312335584
1116	7	-0.2663545931031379
1116	8	-0.3371578956059196
1116	9	-0.37357563145208417
1116	10	-0.3368051206115368
1116	11	-0.27607078594147083
1116	12	-0.2790052531361127
1116	13	-0.38657979154574207
1116	14	-0.5668723637296793
1116	15	-0.5574452809371451
1116	16	-0.6060587300665219
1116	17	-0.6119285405154216
1116	18	-0.6378596309278848
1116	19	-0.6052395608691157
1116	20	-0.6511119688471751
1116	21	-0.6853442712534132
1116	22	-0.5688281671731303
1116	23	-0.5265369680607037
1116	24	-0.6048860638413558
1116	25	-0.5905073153489724
1201	1	0.3622088047067429
1201	2	0.4820790973714786
1201	3	0.3575272220426733
1201	4	0.4828564550828389
1201	5	0.39942652153645497
1201	6	0.4120893906003076
1201	7	0.3704070141946545
1201	8	0.37413086768700765
1201	9	0.3673926142532764
1201	10	0.4027774380756597
1201	11	0.3204984612264212
1201	12	0.3780458859035747
1201	13	0.4285065969919728
1201	14	0.6460202269349011
1201	15	0.542705929989701
1201	16	0.5923250482235574
1201	17	0.5147539909042251
1201	18	0.5356313583881942
1201	19	0.5832138736020198
1201	20	0.5617682274791491
1201	21	0.563893476943214
1201	22	0.5883022956314647
1201	23	0.5877710560338869
1201	24	0.5634811060354394
1201	25	0.5283692618305693
1202	1	0.47704562884437607
1202	2	0.41573255912211593
1202	3	0.39542227918911266
1202	4	0.43902228900543605
1202	5	0.37801282502218925
1202	6	0.4003627492597772
1202	7	0.4068256801466674
1202	8	0.35363458283651283
1202	9	0.35595450644488125
1202	10	0.39081308767762124
1202	11	0.3136615230027928
1202	12	0.33990166924446186
1202	13	0.35759171578909843
1202	14	0.5255609901367393
1202	15	0.5316353130296108
1202	16	0.48753577426851535
1202	17	0.5266702374318655
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2210	13	0.043712348986112204
2210	14	0.011247046369661007
2210	15	0.03777016629793195
2210	16	0.0656936978280882
2210	17	0.03828196992891009
2210	18	0.03692416233076301
2210	19	0.11392498971830634
2210	20	0.018903958336004223
2210	21	0.03139038307208608
2210	22	0.08189181109676014
2210	23	0.152671791029519
2210	24	0.10366833383088903
2210	25	0.08627016315756464
2211	1	-0.17541627482359345
2211	2	-0.13539261897631505
2211	3	-0.17336250515024432
2211	4	-0.24631077138935353
2211	5	-0.08694929219306147
2211	6	-0.15365637131132814
2211	7	-0.19473157695360754
2211	8	-0.1357336267368794
2211	9	-0.15755429341137273
2211	10	-0.14705929606674317
2211	11	-0.15758069307328526
2211	12	-0.2053994464873128
2211	13	-0.22252946450329603
2211	14	-0.26293148465814653
2211	15	-0.20717129232436093
2211	16	-0.2483191554779438
2211	17	-0.22538117309564143
2211	18	-0.25524753856037563
2211	19	-0.3230837905174013
2211	20	-0.2966073512459033
2211	21	-0.17396147581375487
2211	22	-0.1980306009397026
2211	23	-0.1979479900605341
2211	24	-0.20605542357109385
2211	25	-0.15191210287737533
2212	1	-0.08022598987427187
2212	2	-0.135898203368626
2212	3	-0.09807924487504494
2212	4	-0.1656257014006144
2212	5	-0.10757598092524034
2212	6	-0.014293018638042554
2212	7	-0.09164995914377982
2212	8	-0.12376373743087044
2212	9	0.0015752882824813241
2212	10	0.029712784130062175
2212	11	-0.07245328179086385
2212	12	-0.08380269828967357
2212	13	-0.02229407726002819
2212	14	-0.14166991042721833
2212	15	-0.16340743549723413
2212	16	-0.11627278476825609
2212	17	-0.21750000462724017
2212	18	-0.2010659327470492
2212	19	-0.15144410090028515
2212	20	-0.14357765967622527
2212	21	-0.17569632421874815
2212	22	-0.14498640315117228
2212	23	-0.2734007823570792
2212	24	-0.18856388511380828
2212	25	-0.17155133973983538
2213	1	-0.13988655906132053
2213	2	-0.11733891638681371
2213	3	-0.12893189134130978
2213	4	-0.13943956149213932
2213	5	-0.11699071627861457
2213	6	-0.14300387535394066
2213	7	-0.07758706304110063
2213	8	-0.0977994150249657
2213	9	-0.09190661274354994
2213	10	-0.1630749782150005
2213	11	-0.14975584753556603
2213	12	-0.047197806963573896
2213	13	-0.17885553905404805
2213	14	-0.17386013311197246
2213	15	-0.18461185450215112
2213	16	-0.23811730570916723
2213	17	-0.12986040970269386
2213	18	-0.13104164956781972
2213	19	-0.186281123408925
2213	20	-0.17235556198313162
2213	21	-0.1275869431729717
2213	22	-0.1609956227538376
2213	23	-0.16531895338004432
2213	24	-0.1505313563275621
2213	25	-0.14999167917064682
2214	1	-0.1898445380382654
2214	2	-0.14087024131235637
2214	3	-0.190257945809158
2214	4	-0.08187344781855188
2214	5	-0.0999954971779502
2214	6	-0.07623031938982194
2214	7	-0.10818535639621985
2214	8	-0.13742819646905957
2214	9	-0.18434569569968318
2214	10	-0.1334662844326786
2214	11	-0.16856495919859782
2214	12	-0.16425905910045913
2214	13	-0.1488351467622664
2214	14	-0.1754710883241941
2214	15	-0.20059014692547095
2214	16	-0.3211038984044947
2214	17	-0.21154112518900803
2214	18	-0.2484689057114906
2214	19	-0.2546862419100009
2214	20	-0.168478697020241
2214	21	-0.17701226760394206
2214	22	-0.23928105749671147
2214	23	-0.2552869493087542
2214	24	-0.1415828495522362
2214	25	-0.23618226287759825
2215	1	-0.264174273660128
2215	2	-0.15432861912289297
2215	3	-0.23882112038005943
2215	4	-0.24559501770847447
2215	5	-0.26687127729395854
2215	6	-0.2131062971822857
2215	7	-0.24159055924050676
2215	8	-0.15924207561532455
2215	9	-0.29674664567624376
2215	10	-0.25745728733004825
2215	11	-0.26632104904537357
2215	12	-0.2997641506811419
2215	13	-0.29538682221141244
2215	14	-0.4580946219884936
2215	15	-0.5708124482182697
2215	16	-0.5492554258786981
2215	17	-0.41148506979441635
2215	18	-0.4192163203858641
2215	19	-0.35896438397724495
2215	20	-0.35124385174442807
2215	21	-0.34776575376941565
2215	22	-0.4090615519137657
2215	23	-0.3468118537180658
2215	24	-0.43993563989658924
2215	25	-0.44688220696340153
2216	1	-0.20826877325268356
2216	2	-0.1925943758135098
2216	3	-0.18557207333173054
2216	4	-0.13814844543798444
2216	5	-0.15433444334635027
2216	6	-0.20181863129273836
2216	7	-0.20264495955358797
2216	8	-0.30444564874961344
2216	9	-0.32591835977915196
2216	10	-0.38349105162110675
2216	11	-0.3515287674067977
2216	12	-0.2884170741605061
2216	13	-0.3331497448282619
2216	14	-0.5315354952153513
2216	15	-0.5575144759037514
2216	16	-0.5101954140609379
2216	17	-0.48073662457642286
2216	18	-0.6088808659492457
2216	19	-0.5326013830902738
2216	20	-0.6019622183189597
2216	21	-0.5456455087118215
2216	22	-0.5645626673918116
2216	23	-0.49463264859755185
2216	24	-0.5844330761637195
2216	25	-0.5814562655655848
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24389.0
3	663.0
4	502.0
5	344.0
6	281.0
7	431.0
8	969.0
9	2463.0
10	5182.0
11	9222.0
12	15034.0
13	21747.0
14	27992.0
15	33711.0
16	36131.0
17	35743.0
18	34288.0
19	31540.0
20	29167.0
21	27583.0
22	25997.0
23	24763.0
24	23713.0
25	22279.0
26	20564.0
27	20658.0
28	21232.0
29	22784.0
30	27100.0
31	34142.0
32	44406.0
33	62320.0
34	92514.0
35	164085.0
36	392259.0
37	2877331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.55668276645589	18.08713956163814	11.107659923710175	23.248517748195795
2	18.286708104231415	21.275759024623476	34.12063112598614	26.316901745158976
3	20.30086995806178	27.5836344144366	27.08417642018054	25.03131920732108
4	13.947333389258418	19.32257038374884	33.1085097184172	33.62158650857554
5	13.400018065957953	37.89850850701107	29.599948956499755	19.10152447053122
6	30.439739565338954	34.647842799426684	18.388583814304326	16.52383382093004
7	26.960228161452456	32.85375424978884	20.052054167091534	20.13396342166717
8	27.57774859944814	33.35324284654613	19.322242277680154	19.746766276325577
9	26.626047623984267	19.609743792844316	19.421705350066336	34.342503233105084
10	18.54027877672279	30.306774786025382	27.81821730137463	23.334729135877197
11	30.415042575942465	27.08763544176064	20.61168217735023	21.885639804946663
12	25.111446560952988	29.262721964450332	25.302006384482695	20.32382509011399
13	27.969407508762007	23.72719203030106	22.59909570747704	25.704304753459894
14	23.584684133971905	25.230846627543073	24.07421872853822	27.1102505099468
15	26.795897984227462	27.765025368257177	22.110241494724132	23.328835152791225
16	22.266998078600615	28.512382086175382	25.274093211278455	23.946526623945548
17	24.493898699497336	28.76700616912457	23.61574416378653	23.12335096759156
18	23.83967121251845	26.832631565374736	25.54342245953552	23.784274762571293
19	24.53100534022378	27.328761137802417	26.550267011189018	21.589966510784787
20	25.533591684058415	27.18600464114253	24.895977891406243	22.38442578339281
21	23.99265382837996	26.84103873338245	26.333350448926073	22.832956989311512
22	24.385423557987405	27.355476316147726	23.469810342892217	24.789289782972656
23	23.23188964547261	28.690925438363905	24.557861933583382	23.5193229825801
24	23.557133093278555	29.252776345088343	24.54894171962707	22.641148842006036
25	23.55520858764685	26.536189002380734	24.063270256414913	25.845332153557504
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	56950.0
1	56950.0
2	41855.5
3	26761.0
4	26761.0
5	26761.0
6	26446.5
7	26132.0
8	26132.0
9	26132.0
10	24893.0
11	23654.0
12	23654.0
13	23654.0
14	27659.0
15	31664.0
16	31664.0
17	31664.0
18	42405.5
19	53147.0
20	53147.0
21	53147.0
22	72763.0
23	92379.0
24	92379.0
25	92379.0
26	118609.5
27	144840.0
28	144840.0
29	144840.0
30	171899.0
31	198958.0
32	198958.0
33	198958.0
34	216233.5
35	233509.0
36	233509.0
37	233509.0
38	256570.5
39	279632.0
40	279632.0
41	279632.0
42	311476.0
43	343320.0
44	343320.0
45	343320.0
46	490201.0
47	637082.0
48	637082.0
49	637082.0
50	600705.0
51	564328.0
52	564328.0
53	564328.0
54	516470.5
55	468613.0
56	468613.0
57	468613.0
58	426065.0
59	383517.0
60	383517.0
61	383517.0
62	338875.0
63	294233.0
64	294233.0
65	294233.0
66	238236.5
67	182240.0
68	182240.0
69	182240.0
70	139843.5
71	97447.0
72	97447.0
73	97447.0
74	72055.5
75	46664.0
76	46664.0
77	46664.0
78	32761.5
79	18859.0
80	18859.0
81	18859.0
82	12730.5
83	6602.0
84	6602.0
85	6602.0
86	4340.5
87	2079.0
88	2079.0
89	2079.0
90	1366.0
91	653.0
92	653.0
93	653.0
94	409.5
95	166.0
96	166.0
97	166.0
98	133.0
99	100.0
100	100.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.668655656576708
2	0.724547048329322
3	0.7118498531753311
4	0.6968030835909756
5	0.685007745289044
6	0.6004467988709701
7	0.616300493007168
8	0.6230169532475034
9	0.6443767207962732
10	0.6447801830721944
11	0.6549616722704412
12	0.6512830456370421
13	0.6472721559528841
14	0.6481028135797807
15	0.6500963918843325
16	0.640721827237928
17	0.6223998932960945
18	0.6060240715087045
19	0.600802794996783
20	0.6217591002696314
21	0.6245121369759173
22	0.6289027558609422
23	0.6447564499971401
24	0.6536088869923524
25	0.6508321172110124
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4213529.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.460341456207416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.23135454482022	27.8073188800576
2	14.749257593494052	11.050244513556946
3	4.44171191294671	4.9916413472726395
4	1.7715022416534183	2.6544431545109566
5	0.9839447433000121	1.8429453029029406
6	0.6372315992148189	1.4322547975963336
7	0.45259191414246736	1.1867973350866736
8	0.3294805036604046	0.9873961736225565
9	0.24999420604410846	0.8428381488437198
>10	1.7481833631555699	12.999224137361118
>50	0.22881981972739648	6.02749243682931
>100	0.15961536097368215	11.174230261994628
>500	0.00958420905789004	2.39136254059658
>1k	0.004696897149908863	3.3784986203221234
>5k	0.0010155453297100245	2.5067982802754663
>10k+	0.0010155453297100245	8.726514069170372
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	72506	1.7207903398789945	No Hit
CCCATGTACTCTGCGTTGATACCAC	46022	1.092243580143865	No Hit
GAGTACATGGGAAGCAGTGGTATCA	31148	0.7392378217878647	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	30240	0.7176881896386615	No Hit
GCTTCCCATGTACTCTGCGTTGATA	25728	0.6106045549941629	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	22857	0.5424668965135876	No Hit
GTATCAACGCAGAGTACATGGGAAG	19876	0.4717185997770515	No Hit
GCGTTGATACCACTGCTTCCCATGT	17822	0.4229708636157482	No Hit
CATGTACTCTGCGTTGATACCACTG	14808	0.3514393754024239	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	14702	0.34892366944667996	No Hit
GTATCAACGCAGAGTACTTTTTTTT	14494	0.34398718983540877	No Hit
TATCAACGCAGAGTACATGGGAAGC	14390	0.34151895002977317	No Hit
GGTATCAACGCAGAGTACATGGGAA	11658	0.27668018898173	No Hit
TATCAACGCAGAGTACTTTTTTTTT	10431	0.24755970589024068	No Hit
GGTATCAACGCAGAGTACTTTTTTT	10282	0.2440234777071666	No Hit
GTGGTATCAACGCAGAGTACATGGG	10056	0.23865980274492	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	9433	0.22387409698616054	No Hit
ACTCTGCGTTGATACCACTGCTTCC	9264	0.21986320730200265	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	7976	0.18929500663220783	No Hit
CATGGGAAGCAGTGGTATCAACGCA	7595	0.180252705036562	No Hit
CAGTGGTATCAACGCAGAGTACATG	7467	0.17721487142962586	No Hit
GCAGTGGTATCAACGCAGAGTACAT	6696	0.15891667056284647	No Hit
CCACTGCTTCCCATGTACTCTGCGT	6580	0.15616363385656062	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	6491	0.15405139017673783	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	5828	0.13831636141581083	No Hit
ACATGGGAAGCAGTGGTATCAACGC	5811	0.13791289913988963	No Hit
GTACTCTGCGTTGATACCACTGCTT	5701	0.13530226088392888	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	5640	0.13385454330562338	No Hit
ATACCACTGCTTCCCATGTACTCTG	5451	0.12936899212038175	No Hit
GATACCACTGCTTCCCATGTACTCT	5307	0.1259514293125786	No Hit
GGTATCAACGCAGAGTACATGGGGG	5112	0.12132347967701183	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5079	0.12054028820022361	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	4591	0.10895854757377961	No Hit
TACCACTGCTTCCCATGTACTCTGC	4469	0.10606311241716859	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	4304	0.1021471550332275	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.3733075054188543E-5	0.0
2	0.0	0.0	0.0	2.3733075054188543E-5	0.0
3	0.0	0.0	0.0	2.3733075054188543E-5	0.0
4	0.0	0.0	0.0	2.3733075054188543E-5	0.0
5	0.0	0.0	0.0	2.3733075054188543E-5	0.0
6	0.0	0.0	0.0	4.7466150108377086E-5	0.0
7	0.0	0.0	0.0	4.7466150108377086E-5	0.0
8	0.0	0.0	0.0	4.7466150108377086E-5	0.0
9	0.0	0.0	0.0	4.7466150108377086E-5	0.0
10	0.0	0.0	0.0	4.7466150108377086E-5	0.0
11	0.0	0.0	0.0	4.7466150108377086E-5	0.0
12	0.0	0.0	0.0	4.7466150108377086E-5	0.0
13	0.0	0.0	0.0	7.119922516256563E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	760	0.0	16.882069	2
CGCACGA	595	0.0	16.124327	12
TGTCACG	510	0.0	16.018278	7
ATAACGC	875	0.0	15.531104	3
GTCACGC	545	0.0	15.512473	8
ACGCACG	525	0.0	15.379309	11
GTATAAC	870	0.0	15.293212	1
ACGATTT	635	0.0	15.256937	15
TATCACG	365	0.0	15.102215	2
CGGCAAT	335	0.0	14.741876	16
CACGCAC	580	0.0	14.576029	10
TAACGCA	965	0.0	14.477046	4
TTTCCCG	685	0.0	14.144135	19
TGTCGCG	115	2.5465852E-11	14.042314	7
GTCGGCA	345	0.0	14.040971	14
CGATTTC	725	0.0	14.016513	16
CGACTCG	170	0.0	13.977443	5
TACGCCC	490	0.0	13.964531	4
CACGATT	695	0.0	13.939957	14
GCTACGC	495	0.0	13.63197	2
>>END_MODULE