FastQCFastQC Report
Wed 25 May 2016
SRR1295358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295358_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4213529
Sequences flagged as poor quality0
Sequence length25
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCANTGGTATCAAC376450.8934316104149277No Hit
CCCATGTACTCTGCNTTGATACCAC258440.6133575917004488No Hit
GTACTTTTTTTTTTNTTTTTTTTTT223080.529437438308838No Hit
GTATCAACGCAGAGNACTTTTTTTT216900.5147703979253495No Hit
TATCAACGCAGAGTNCTTTTTTTTT172650.4097515408105652No Hit
GAGTACATGGGAAGNAGTGGTATCA161690.3837400905511746No Hit
GGTATCAACGCAGANTACTTTTTTT150350.35682678343972474No Hit
GCTTCCCATGTACTNTGCGTTGATA143390.34030856320200953No Hit
GTATCAACGCAGAGNACATGGGAAG104810.24874635964295014No Hit
GCGTTGATACCACTNCTTCCCATGT89290.2119126271588495No Hit
ACGCAGAGTACATGNGAAGCAGTGG87530.20773560594931234No Hit
TATCAACGCAGAGTNCATGGGAAGC80330.19064779191029657No Hit
CATGTACTCTGCGTNGATACCACTG78260.18573504537407953No Hit
ACGCAGAGTACTTTNTTTTTTTTTT71450.16957282126217715No Hit
GAGTACTTTTTTTTNTTTTTTTTTT68350.1622155679953787No Hit
GGTATCAACGCAGANTACATGGGAA58590.13905208674249067No Hit
ACTCTGCGTTGATANCACTGCTTCC53120.12607009468784952No Hit
GGGAAGCAGTGGTANCAACGCAGAG51950.12329332490650947No Hit
GTGGTATCAACGCANAGTACATGGG46510.11038253207703091No Hit
CATGGGAAGCAGTGNTATCAACGCA46420.11016893440154321No Hit
CAGTGGTATCAACGNAGAGTACATG43080.10224208733344425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTT3300.022.98491515
CGCACGA3300.022.51583512
TCGCGCA807.70342E-921.2839939
CACGATT3550.020.93021214
CACGCAC3150.018.18242810
CGTCGTA955.5388227E-817.92378810
CGCGCAC1059.0130925E-917.69101110
CGCGTGG803.836183E-617.4145912
AGTACCC3500.017.24873513
ACGGTAC1353.8198777E-1117.19959411
CCTGTCG2500.016.71800611
CGTGTCG652.7053646E-416.6699819
TCGGCAA2450.016.42736815
ACGCACG3500.016.36418511
AAGTACC3950.015.6755812
TGCGCGT1001.8567898E-615.47963510
TGTCGGC2700.015.47963513
CGACGTA901.1752549E-515.4792699
GTCGGCA2650.015.18756714
GTACCCC4000.015.09264514
Produced by FastQC (version 0.11.5)