FastQCFastQC Report
Wed 25 May 2016
SRR1295357_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295357_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11411208
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNN612120.5364199828799896No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA272490.23879154599583144No Hit
GTACATGGGAAGCAGTGGTATCAAC268880.23562798960460626No Hit
GTATCAACGCAGAGTACTTTTTTTT255280.22370988242436737No Hit
TATCAACGCAGAGTACTTTTTTTTT175890.15413793175972254No Hit
GGTATCAACGCAGAGTACTTTTTTT166090.14554988393866802No Hit
CCCATGTACTCTGCGTTGATACCAC138930.12174872283460261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGA14450.016.3676912
TATAACG8350.014.9125212
ATAACGC9600.014.4557373
TGTCACG12600.014.3989287
CGATTTC17100.014.16145816
ACGATTT16650.014.14700815
GTCACGC13050.013.8294438
TATCACG7350.013.5790132
TTTCCCG17450.013.55387919
CGCGCAC4450.013.44704310
CACGATT18400.013.317986514
TGTCGCG4250.013.1865277
GTCGCGC4500.013.0870218
CGACTCG3850.013.0851155
TGCGCGT4900.012.79363910
GTATAAC12200.012.4657591
AGCCCGG20900.012.4071556
TACGCCC10000.012.3563024
TAGCCCG6600.012.2415785
GTCACGG10750.012.2019491
Produced by FastQC (version 0.11.5)