FastQCFastQC Report
Wed 25 May 2016
SRR1295357_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295357_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11411208
Sequences flagged as poor quality0
Sequence length25
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGNACTTTTTTTT332980.2918008330055854No Hit
GTACTTTTTTTTTTNTTTTTTTTTT249580.21871479338559074No Hit
TATCAACGCAGAGTNCTTTTTTTTT237820.20840913600032532No Hit
GGTATCAACGCAGANTACTTTTTTT213780.18734212889643234No Hit
GTACATGGGAAGCANTGGTATCAAC146180.12810212555936235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGA9100.022.4710812
TCGCGCA2550.019.439549
ACGATTT11300.018.23327315
CACGATT11650.018.21738614
CGGCCTT15200.017.83552410
TGCGCGT3050.017.77704610
CGTCGTA2050.015.86926610
ACGCACG10300.015.64182911
CCGGCCT17650.015.5347679
GCGAATT1001.8434694E-615.49142614
CGCGCAC3300.014.55255110
CACGCAC11650.014.36115210
TATAACG12800.013.92789652
ACGCGAC800.001300243513.55499711
CTGCGCG4350.013.1761549
CGCGTGG4250.013.12214912
GCGCGTG4400.013.02688111
TGTCGCG3950.012.9512787
TCGTCTA3400.012.75764714
ATAACGC14700.012.6022543
Produced by FastQC (version 0.11.5)