FastQCFastQC Report
Wed 25 May 2016
SRR1295356_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295356_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7713719
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1792712.324054065231051No Hit
CCCATGTACTCTGCGTTGATACCAC1135961.4726489258942412No Hit
GAGTACATGGGAAGCAGTGGTATCA765360.9922062237423998No Hit
GCTTCCCATGTACTCTGCGTTGATA764520.9911172548546298No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA552800.7166452394752778No Hit
GCGTTGATACCACTGCTTCCCATGT470970.6105615203250209No Hit
GTATCAACGCAGAGTACATGGGAAG458380.5942399509238022No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN407960.5288758898269433No Hit
CATGTACTCTGCGTTGATACCACTG346580.44930337752775285No Hit
TATCAACGCAGAGTACATGGGAAGC321730.4170880479312249No Hit
ACGCAGAGTACATGGGAAGCAGTGG314610.4078577402158414No Hit
GGTATCAACGCAGAGTACATGGGAA233980.3033296909052559No Hit
GGGAAGCAGTGGTATCAACGCAGAG231100.29959608329004467No Hit
CTGCTTCCCATGTACTCTGCGTTGA228290.2959532230821475No Hit
GTATCAACGCAGAGTACTTTTTTTT222150.28799337906916234No Hit
ACTCTGCGTTGATACCACTGCTTCC204250.2647879706273978No Hit
GTGGTATCAACGCAGAGTACATGGG201660.26143031655677373No Hit
CATGGGAAGCAGTGGTATCAACGCA177450.23004467754140384No Hit
CAGTGGTATCAACGCAGAGTACATG173810.22532581236106736No Hit
CCACTGCTTCCCATGTACTCTGCGT169500.219738364853581No Hit
GCAGTGGTATCAACGCAGAGTACAT161140.20890053163720382No Hit
ATGGGAAGCAGTGGTATCAACGCAG154150.19983875482111807No Hit
TATCAACGCAGAGTACTTTTTTTTT153840.19943687344586963No Hit
GATACCACTGCTTCCCATGTACTCT148700.19277342096594394No Hit
GGTATCAACGCAGAGTACTTTTTTT143290.18575994277209215No Hit
GTACTCTGCGTTGATACCACTGCTT137140.1777871348437764No Hit
GCAGAGTACATGGGAAGCAGTGGTA136210.1765814907180311No Hit
ATACCACTGCTTCCCATGTACTCTG133130.1725886047962079No Hit
ACATGGGAAGCAGTGGTATCAACGC114080.1478923460914249No Hit
AAGCAGTGGTATCAACGCAGAGTAC113780.14750342863150706No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA113350.14694598027229147No Hit
TACCACTGCTTCCCATGTACTCTGC110930.14380871276228757No Hit
GGAAGCAGTGGTATCAACGCAGAGT101150.1311300035689659No Hit
TGGGAAGCAGTGGTATCAACGCAGA97500.12639817447329882No Hit
GCTTTCCATGTACTCTGCGTTGATA92640.12009771162262976No Hit
CCATGTACTCTGCGTTGATACCACT91860.11908652622684338No Hit
CTTCCCATGTACTCTGCGTTGATAC87760.11377132094129952No Hit
ATGGAAAGCAGTGGTATCAACGCAG85180.11042663078600608No Hit
GTTGATACCACTGCTTCCCATGTAC83950.10883206920034293No Hit
GTACTTTTTTTTTTTTTTTTTTTTT82720.10723750761467976No Hit
GGTATCAACGCAGAGTACATGGGGG81400.10552627079104125No Hit
ATCAACGCAGAGTACATGGGAAGCA79210.10268717333364102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG14900.016.3320222
ATAACGC16000.015.6243583
CGCACGA6650.015.42581612
GTATAAC17350.015.3403861
ACGATTT6750.015.33729915
ACGCACG4850.015.07948511
CACGATT7000.014.79011914
TAACGCA18900.014.5847784
TGTCACG5300.014.1584957
TGCGCGT2350.014.1460410
TATCACG7550.014.1012412
GTCACGC5450.013.7682738
CGATTTC7800.013.75829616
CGCGGAA1400.013.56891614
TGTCGCG2250.013.50931557
TCGAATA2050.012.97286918
GGCTACG5250.012.6740831
AACGAGC2750.012.4433183
TACAACG5650.012.2817622
TACGCCC4850.012.1510424
Produced by FastQC (version 0.11.5)