##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295356_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7713719
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.187916879004796	33.0	33.0	33.0	27.0	33.0
2	31.006088632474167	33.0	33.0	33.0	27.0	33.0
3	31.158943435714992	33.0	33.0	33.0	27.0	33.0
4	34.60500401946195	37.0	37.0	37.0	27.0	37.0
5	34.89229462468104	37.0	37.0	37.0	33.0	37.0
6	34.865679317589866	37.0	37.0	37.0	33.0	37.0
7	34.781340492180234	37.0	37.0	37.0	33.0	37.0
8	34.76938361379252	37.0	37.0	37.0	33.0	37.0
9	34.61678407004455	37.0	37.0	37.0	33.0	37.0
10	34.71182512611621	37.0	37.0	37.0	33.0	37.0
11	34.682818884120614	37.0	37.0	37.0	33.0	37.0
12	34.68254340091984	37.0	37.0	37.0	33.0	37.0
13	34.54227422596026	37.0	37.0	37.0	27.0	37.0
14	36.50387951648226	40.0	37.0	40.0	27.0	40.0
15	36.524047479562064	40.0	37.0	40.0	27.0	40.0
16	36.453733925231134	40.0	37.0	40.0	27.0	40.0
17	36.5188314482288	40.0	37.0	40.0	27.0	40.0
18	36.52241039633412	40.0	37.0	40.0	27.0	40.0
19	36.5141041565035	40.0	37.0	40.0	27.0	40.0
20	36.54975414582771	40.0	37.0	40.0	27.0	40.0
21	36.537620698913194	40.0	37.0	40.0	27.0	40.0
22	36.59017135573645	40.0	37.0	40.0	27.0	40.0
23	36.591988637387495	40.0	37.0	40.0	27.0	40.0
24	36.64098044017419	40.0	37.0	40.0	27.0	40.0
25	36.61078929631738	40.0	37.0	40.0	27.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31954226448350553
1101	2	0.28771371822010394
1101	3	0.3090789778299232
1101	4	0.35263253829015895
1101	5	0.2837376097388642
1101	6	0.3398880888235354
1101	7	0.268291458112941
1101	8	0.3050018043807299
1101	9	0.2700370406480346
1101	10	0.2810963007085334
1101	11	0.21432270185629676
1101	12	0.2799126982688378
1101	13	0.2563677059157996
1101	14	0.46590162961159365
1101	15	0.4734950415991861
1101	16	0.49486705766704375
1101	17	0.5377936153058585
1101	18	0.5369318308352717
1101	19	0.5417103739225411
1101	20	0.48593865520608404
1101	21	0.45852682061748595
1101	22	0.42984505958030184
1101	23	0.44243488284701726
1101	24	0.49782706665777
1101	25	0.4922863612306472
1102	1	0.23735328996029637
1102	2	0.24991085512075628
1102	3	0.1802081362303838
1102	4	0.2657386261775869
1102	5	0.2575086644820317
1102	6	0.23458310200449262
1102	7	0.24786471359745832
1102	8	0.21715358207718793
1102	9	0.2423896578590643
1102	10	0.275663392216579
1102	11	0.24625923025443086
1102	12	0.21896284335234384
1102	13	0.24076369250794016
1102	14	0.35020474027741955
1102	15	0.33136238977704124
1102	16	0.37232127561543393
1102	17	0.3884201071794706
1102	18	0.3648395894976133
1102	19	0.35245347685540906
1102	20	0.4123521047830039
1102	21	0.4628661633167752
1102	22	0.39426450952772285
1102	23	0.4374410118508223
1102	24	0.39131651450735205
1102	25	0.34919496238633485
1103	1	0.09542825629478102
1103	2	0.07080438309546722
1103	3	0.06646242631623878
1103	4	0.06520606741606372
1103	5	0.08192707604693794
1103	6	0.13198733949184316
1103	7	0.11158757116405127
1103	8	0.09164859091788458
1103	9	0.010025176005520109
1103	10	0.046403933927116725
1103	11	0.06296587625220695
1103	12	0.10558846080177631
1103	13	0.07229664356103882
1103	14	0.27221408936839
1103	15	0.21039559204508862
1103	16	0.24116859643126531
1103	17	0.25049094068510414
1103	18	0.208337518324754
1103	19	0.2520291191757167
1103	20	0.19048811999130777
1103	21	0.18955117214630235
1103	22	0.1530848129249378
1103	23	0.1913327776462168
1103	24	0.252987825644837
1103	25	0.1788971178089156
1104	1	0.16670807227859186
1104	2	0.12904575263882023
1104	3	0.1405077184942769
1104	4	0.11025947577727635
1104	5	0.08941574621047721
1104	6	0.10093358906319594
1104	7	0.05105753051596906
1104	8	0.13007538143639152
1104	9	0.14203693886651791
1104	10	0.15920152971605006
1104	11	0.1450352931123433
1104	12	0.1108472344484781
1104	13	0.13246202977775567
1104	14	0.18770831398079935
1104	15	0.3302059874273482
1104	16	0.28378839141542045
1104	17	0.2727275350351661
1104	18	0.2387603514210923
1104	19	0.19931566483314356
1104	20	0.21517166964999745
1104	21	0.1703336508175255
1104	22	0.18105364009839064
1104	23	0.1489665377695033
1104	24	0.21806423920079254
1104	25	0.16879906954743973
1105	1	0.14550547968682892
1105	2	0.1334591648484782
1105	3	0.1035012201827108
1105	4	0.13416755093428634
1105	5	0.11560049542918449
1105	6	0.09471486933706075
1105	7	0.11159159325855939
1105	8	0.14170168174288023
1105	9	0.11345513759592052
1105	10	0.12137824310688217
1105	11	0.09867514991737636
1105	12	0.16572724559920715
1105	13	0.20517017938497872
1105	14	0.20272594999868687
1105	15	0.21446662614928158
1105	16	0.20659194951475257
1105	17	0.2110477897173766
1105	18	0.25330240461718034
1105	19	0.16206949755002142
1105	20	0.16983418763227576
1105	21	0.15633640314028696
1105	22	0.17663505636200227
1105	23	0.17324181602955235
1105	24	0.266165375224638
1105	25	0.18481586429462027
1106	1	0.11313960482149099
1106	2	0.0964384237361422
1106	3	0.09306923196589878
1106	4	0.15383484512369705
1106	5	0.04960810603448351
1106	6	0.06209819155956353
1106	7	0.060850926698371666
1106	8	0.10785466875417882
1106	9	0.09000482156250911
1106	10	0.16738400673889942
1106	11	0.12210892317195032
1106	12	0.15008432200781385
1106	13	0.10843672768206858
1106	14	0.14630055328806435
1106	15	0.1858832936271
1106	16	0.14404235214601613
1106	17	0.24824625781450038
1106	18	0.19072047290874394
1106	19	0.1985782196782111
1106	20	0.19424032464839058
1106	21	0.15009925606029384
1106	22	0.17877892058219658
1106	23	0.20226528236890573
1106	24	0.16216392156989912
1106	25	0.14112115084088828
1107	1	0.08734849335841233
1107	2	0.09680240617194613
1107	3	0.11058796919282443
1107	4	0.08749082001975239
1107	5	0.10828459445820471
1107	6	0.079636357796943
1107	7	0.05478778324508227
1107	8	0.05045149583524733
1107	9	0.06319546381268282
1107	10	0.11804078113181049
1107	11	0.07240702234093987
1107	12	0.1145671678991178
1107	13	0.141176779336476
1107	14	0.1487843284490964
1107	15	0.0671639173934011
1107	16	0.06698948105514546
1107	17	0.08689963263822875
1107	18	0.11501263390758254
1107	19	0.11317475066300631
1107	20	0.10165670560245843
1107	21	0.1365591279082068
1107	22	0.12248093992555198
1107	23	0.12234600921510719
1107	24	0.15993036991171294
1107	25	0.16210789720987862
1108	1	-0.010477466622393905
1108	2	0.02021289289015371
1108	3	0.07870904136884249
1108	4	0.0449423422761015
1108	5	0.04709505955760562
1108	6	0.03556825436966449
1108	7	0.029872629238809623
1108	8	0.022634207829945296
1108	9	0.008151103740573262
1108	10	0.050148455741087616
1108	11	0.04061500594952605
1108	12	0.005838407545475377
1108	13	0.06302037751596146
1108	14	0.03789839570099218
1108	15	0.04664166578766782
1108	16	-0.14115607241952688
1108	17	-0.07698970173746744
1108	18	0.00590626122659188
1108	19	-0.06490617849800628
1108	20	-0.007551007152152067
1108	21	-0.05093114671984722
1108	22	-0.010205024354263514
1108	23	-0.13113973011072488
1108	24	-0.0868959554034916
1108	25	0.007332981444612585
1109	1	-0.089687117968964
1109	2	-0.03598891147547434
1109	3	0.008085373021732067
1109	4	-0.006867797982081925
1109	5	-0.04486039915722273
1109	6	-0.03469912283211585
1109	7	0.0156771014913204
1109	8	-0.022575587388701024
1109	9	-0.012450783400126397
1109	10	-0.03214505902626996
1109	11	-8.521335008566666E-4
1109	12	0.02055696715791555
1109	13	0.018555839344585934
1109	14	-0.007659984282952337
1109	15	-0.044498191217876126
1109	16	-0.08781969317509919
1109	17	-0.09383501594750498
1109	18	0.01416969612137109
1109	19	-0.07512109573340098
1109	20	-0.01702452053029191
1109	21	-0.03842991008897911
1109	22	0.03858617468273451
1109	23	0.043509886723050784
1109	24	0.05137347691155014
1109	25	-0.004487470922548198
1110	1	-0.08709437231133776
1110	2	-0.09917532782271365
1110	3	-0.11491343849903757
1110	4	-0.11568955997781671
1110	5	-0.11166703869324124
1110	6	-0.11808228342386684
1110	7	-0.08981712105124018
1110	8	-0.10006500999007528
1110	9	-0.12984759800075096
1110	10	-0.09963468735050185
1110	11	-0.06701785762229662
1110	12	-0.06202289004602335
1110	13	-0.0980410405106582
1110	14	-0.1541132806756309
1110	15	-0.20791615113363093
1110	16	-0.1177386028816727
1110	17	-0.2196576231813694
1110	18	-0.23306536930044075
1110	19	-0.2245251565472941
1110	20	-0.24182803163719768
1110	21	-0.1894513906048374
1110	22	-0.17882146116186703
1110	23	-0.171765190518677
1110	24	-0.26015164420569903
1110	25	-0.2877284443055004
1111	1	-1.159247937502105
1111	2	-1.7741311435754987
1111	3	-1.4533602421309837
1111	4	-1.7886313201769681
1111	5	-1.740625404308446
1111	6	-1.720393361042511
1111	7	-1.6769881474301513
1111	8	-1.6409304922902024
1111	9	-1.620582396188162
1111	10	-1.5671737716730334
1111	11	-1.5654005909314392
1111	12	-1.5849568226552009
1111	13	-1.6023045922006673
1111	14	-1.959770689646092
1111	15	-1.8424781385180893
1111	16	-1.881702057429628
1111	17	-1.905046477199548
1111	18	-1.8985718250303236
1111	19	-1.9759308251613774
1111	20	-1.9809632768033083
1111	21	-2.0567535687533223
1111	22	-2.00561374943355
1111	23	-2.1209612404036733
1111	24	-2.1997429147707734
1111	25	-2.1354112711593416
1112	1	-0.5573054077946367
1112	2	-0.658500478650339
1112	3	-0.5608006298129062
1112	4	-0.7603752112596567
1112	5	-0.7728918417078532
1112	6	-0.6576030189322069
1112	7	-0.6154869194405137
1112	8	-0.5910067400553061
1112	9	-0.6306870068591763
1112	10	-0.5823538428653876
1112	11	-0.5406384189353091
1112	12	-0.5376251104126908
1112	13	-0.5074225293889612
1112	14	-0.757403946520121
1112	15	-0.8530088371525153
1112	16	-0.8162730525311375
1112	17	-0.9120223682739095
1112	18	-0.8799666023008683
1112	19	-0.7889427573557057
1112	20	-0.808349037122305
1112	21	-0.8750989196769154
1112	22	-0.9061764969279906
1112	23	-0.94753974227649
1112	24	-0.8727800205411285
1112	25	-0.8928214025148264
1113	1	-0.26333911413404465
1113	2	-0.26105781281596663
1113	3	-0.23948894344346883
1113	4	-0.29841489020254386
1113	5	-0.3110387710499154
1113	6	-0.2987217672395275
1113	7	-0.26784457959896457
1113	8	-0.27518245968393984
1113	9	-0.3658139433961267
1113	10	-0.3171655683243273
1113	11	-0.2916717767203707
1113	12	-0.33351491748899065
1113	13	-0.3761211053365372
1113	14	-0.5109924118058515
1113	15	-0.4787428616063636
1113	16	-0.4395675178964069
1113	17	-0.5299861825865335
1113	18	-0.536758028741005
1113	19	-0.47789533491131664
1113	20	-0.5001549213850254
1113	21	-0.5067553247826595
1113	22	-0.44379055774641074
1113	23	-0.38074582303010374
1113	24	-0.41098031045365957
1113	25	-0.4210802004121277
1114	1	-0.1875295769319365
1114	2	-0.20273789851848534
1114	3	-0.16476026231542917
1114	4	-0.20092705650269949
1114	5	-0.16296063620429635
1114	6	-0.19701485515945194
1114	7	-0.17857726967614695
1114	8	-0.13451444409719926
1114	9	-0.1369025380732225
1114	10	-0.14641759496905138
1114	11	-0.15529833986263242
1114	12	-0.18393596044725058
1114	13	-0.14230549068525278
1114	14	-0.34901965469001084
1114	15	-0.29339625385482293
1114	16	-0.29181270190481
1114	17	-0.2750079103151748
1114	18	-0.2994463751902998
1114	19	-0.3223922682182234
1114	20	-0.34423822290052186
1114	21	-0.36080033575664316
1114	22	-0.2571838323634239
1114	23	-0.3086052275913289
1114	24	-0.27761758095355304
1114	25	-0.287020036744714
1115	1	-0.29482539713204403
1115	2	-0.24787579388191716
1115	3	-0.1930026699638958
1115	4	-0.2811829242861208
1115	5	-0.1664349564459897
1115	6	-0.27468669755513986
1115	7	-0.2706313840162764
1115	8	-0.2531765278015925
1115	9	-0.23692947422915722
1115	10	-0.27000834107954574
1115	11	-0.228020213890197
1115	12	-0.24258055083546282
1115	13	-0.3058875041547324
1115	14	-0.48207015449236934
1115	15	-0.49128866902578494
1115	16	-0.4803516362142304
1115	17	-0.4324062420957304
1115	18	-0.4028541610242655
1115	19	-0.4345683218120584
1115	20	-0.4178570498951757
1115	21	-0.5445073488756904
1115	22	-0.48480654489273434
1115	23	-0.42711735189853783
1115	24	-0.5298484460036477
1115	25	-0.4476450770438234
1116	1	-0.10052732420664512
1116	2	-0.08350854109537309
1116	3	-0.004726690676232437
1116	4	-0.031521246954191895
1116	5	-0.1063753265105376
1116	6	-0.16799377873751098
1116	7	-0.0970252418732187
1116	8	-0.22372536041969937
1116	9	-0.24458405212985923
1116	10	-0.273886767986383
1116	11	-0.1943541345723645
1116	12	-0.18589564342811826
1116	13	-0.20200974389925364
1116	14	-0.3608015876722206
1116	15	-0.45170426180406054
1116	16	-0.5037796085344013
1116	17	-0.34545154841796943
1116	18	-0.3759900472960922
1116	19	-0.38815726550934926
1116	20	-0.3761834906331174
1116	21	-0.37724822044756934
1116	22	-0.336612115037326
1116	23	-0.33981526329721135
1116	24	-0.47182522149331874
1116	25	-0.4255019563873432
1201	1	0.33946349730949876
1201	2	0.36990582027431884
1201	3	0.3555216809435855
1201	4	0.3491043687198072
1201	5	0.38111831116412276
1201	6	0.40715830392136354
1201	7	0.30694340643417206
1201	8	0.3127969406126425
1201	9	0.29719246203143257
1201	10	0.38055812417724866
1201	11	0.30607134052453944
1201	12	0.39586015061605195
1201	13	0.36964062886664806
1201	14	0.5446519566767947
1201	15	0.5227835205115028
1201	16	0.5558027603511988
1201	17	0.5082500625631994
1201	18	0.5529865358841732
1201	19	0.4576559132468887
1201	20	0.5438765893388577
1201	21	0.5190892369998679
1201	22	0.5184707192646414
1201	23	0.5104976448077281
1201	24	0.52032390708451
1201	25	0.4839753259273536
1202	1	0.305046437002904
1202	2	0.27705799447671353
1202	3	0.3084453024215783
1202	4	0.3734888210693512
1202	5	0.27710737833031374
1202	6	0.30408233450933864
1202	7	0.3022699553602948
1202	8	0.26692742778277534
1202	9	0.291008298790949
1202	10	0.23256012734505305
1202	11	0.3042465366206173
1202	12	0.24889565561819182
1202	13	0.3094699725603647
1202	14	0.47133635669668905
1202	15	0.44708376030319386
1202	16	0.4447606962792534
1202	17	0.4786601235163843
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2210	8	-0.019481117963231043
2210	9	0.009595909953212356
2210	10	-0.0187400548819312
2210	11	0.024636579494497823
2210	12	0.02148795305082274
2210	13	-0.01937268590739194
2210	14	-0.04966829957841412
2210	15	-0.04563553547443888
2210	16	-0.05181044945632607
2210	17	-0.027155456165338876
2210	18	-0.07718705819206662
2210	19	-0.12916865005819034
2210	20	-0.06763378751374205
2210	21	-0.10932089112773014
2210	22	-0.15114176034104787
2210	23	-0.0970750616169056
2210	24	-0.13216776405083408
2210	25	-0.091746889322458
2211	1	-0.05731156914870539
2211	2	-0.02901861436440001
2211	3	-0.04441574881392896
2211	4	-0.05803854026252253
2211	5	-0.04187088756352608
2211	6	-0.03523275024466699
2211	7	-0.04551233302478863
2211	8	-0.059158423528543835
2211	9	-0.032244582040412695
2211	10	-0.07092225561945753
2211	11	0.004131766686469973
2211	12	-0.037253245118179734
2211	13	-0.06551213168890513
2211	14	-0.1463172444562133
2211	15	-0.12263687686915148
2211	16	-0.07464950793673353
2211	17	-0.05941211204043384
2211	18	-0.008583308472999818
2211	19	-0.06301098082226275
2211	20	-0.0204120279572777
2211	21	-7.853425820130155E-4
2211	22	-0.03831703749626314
2211	23	-0.1236213330008269
2211	24	-0.09617001318223117
2211	25	-0.056172375095755456
2212	1	-0.18613677960656716
2212	2	-0.17151387114537187
2212	3	-0.20068957855714586
2212	4	-0.2006872869929026
2212	5	-0.20783243107609195
2212	6	-0.21506063211336368
2212	7	-0.18588072849522774
2212	8	-0.18544852736966533
2212	9	-0.20802381292168093
2212	10	-0.17257901645989193
2212	11	-0.19090164263265308
2212	12	-0.17357859777006013
2212	13	-0.17197652590840562
2212	14	-0.28536853452790467
2212	15	-0.30491757955802257
2212	16	-0.3084930315761696
2212	17	-0.23479833640625003
2212	18	-0.29893946209445943
2212	19	-0.2696084159911294
2212	20	-0.2413758774925583
2212	21	-0.1853274720221023
2212	22	-0.26112410111965545
2212	23	-0.2794721806884084
2212	24	-0.2447612270795716
2212	25	-0.19321488152601063
2213	1	-0.09628189134198806
2213	2	-0.03461357173087265
2213	3	-0.12247299098804376
2213	4	-0.0533209842309148
2213	5	-0.02110149194076172
2213	6	-0.06579498758078017
2213	7	-0.024267714894328662
2213	8	-0.03812938599524074
2213	9	0.022730678075141952
2213	10	-0.08582298146455258
2213	11	-0.024118116932051237
2213	12	-0.07800797293022299
2213	13	-0.11217091437995919
2213	14	-0.13071384091070115
2213	15	-0.19617710046539827
2213	16	-0.13262018550264543
2213	17	-0.12624918014789444
2213	18	-0.08023816255256833
2213	19	-0.05656015750553678
2213	20	-0.12351400946030111
2213	21	-0.10728402529255021
2213	22	-0.07976182252148334
2213	23	-0.06789392764859059
2213	24	-0.08421103638927718
2213	25	-0.15242457670693454
2214	1	-0.1941309899489987
2214	2	-0.18063877149416996
2214	3	-0.18980250257384057
2214	4	-0.2327237501952979
2214	5	-0.1253408100914939
2214	6	-0.16587887911946808
2214	7	-0.1392529159091751
2214	8	-0.15032481841821266
2214	9	-0.18525646607764656
2214	10	-0.17736061699834238
2214	11	-0.19736468740290292
2214	12	-0.18337876185424307
2214	13	-0.17249751540910552
2214	14	-0.22898943436490526
2214	15	-0.2676934568393605
2214	16	-0.3287369822206614
2214	17	-0.33814933630180377
2214	18	-0.3378987037065926
2214	19	-0.24405828390907658
2214	20	-0.2832772076899417
2214	21	-0.2085714586383105
2214	22	-0.2663043872986748
2214	23	-0.24524776482618904
2214	24	-0.21572050980224589
2214	25	-0.26258136173443347
2215	1	-0.2125386831034426
2215	2	-0.202520356900461
2215	3	-0.20858918980871266
2215	4	-0.190700625609594
2215	5	-0.14600133851874375
2215	6	-0.1885834469769634
2215	7	-0.1588168550626392
2215	8	-0.17852503497131522
2215	9	-0.23673272040154103
2215	10	-0.23321155586337738
2215	11	-0.2675186941100449
2215	12	-0.24904031792451775
2215	13	-0.21208036992285884
2215	14	-0.3888216301597751
2215	15	-0.3826204477636779
2215	16	-0.3736840788901219
2215	17	-0.3515106743476366
2215	18	-0.28240211985879426
2215	19	-0.3670806520108485
2215	20	-0.3450848314808397
2215	21	-0.2973394203469013
2215	22	-0.2883766747409453
2215	23	-0.346550415923069
2215	24	-0.3659521404492523
2215	25	-0.380394492727973
2216	1	0.026475670599864998
2216	2	0.030969192991825878
2216	3	0.016562676289090916
2216	4	0.07779663510004298
2216	5	0.05008984714693554
2216	6	0.04564290828942319
2216	7	0.010094140411972319
2216	8	-0.04274369669491307
2216	9	-0.07488952865958254
2216	10	-0.07007291771319046
2216	11	-0.058120388798769795
2216	12	-0.04753404916912274
2216	13	-0.13846893074667577
2216	14	-0.16638008771991508
2216	15	-0.21813126645592007
2216	16	-0.16891080928718338
2216	17	-0.15999644656676537
2216	18	-0.16837937890279875
2216	19	-0.18039904557874564
2216	20	-0.16029455280220617
2216	21	-0.10391022594320276
2216	22	-0.16834453315211562
2216	23	-0.08458828430075727
2216	24	-0.19273105174112004
2216	25	-0.10423627450229134
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	43540.0
3	1223.0
4	868.0
5	628.0
6	529.0
7	722.0
8	1742.0
9	3918.0
10	8044.0
11	14424.0
12	23966.0
13	34908.0
14	46275.0
15	58089.0
16	63513.0
17	63977.0
18	60427.0
19	55445.0
20	50240.0
21	46721.0
22	43279.0
23	40918.0
24	39025.0
25	36645.0
26	34125.0
27	33485.0
28	34270.0
29	37714.0
30	44668.0
31	57420.0
32	76692.0
33	109047.0
34	166842.0
35	302588.0
36	736744.0
37	5341058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.22220636016563	17.94007300518798	10.84684112870045	22.990879505945944
2	17.438050874865045	21.86610980162466	34.61764990435519	26.078189419155102
3	20.30471710071889	28.749287045221667	27.75594799127888	23.19004786278057
4	13.776849114752753	19.90280103895046	34.04067747673241	32.27967236956438
5	12.588428040069324	38.68497619622812	30.23764878744133	18.488946976261225
6	28.855143660279158	34.70694690544758	19.561849580126932	16.876059854146337
7	25.75229065482737	33.361648204614134	20.84476655490705	20.041294585651443
8	27.10573944202072	33.78467283907462	20.052217298255023	19.057370420649637
9	26.89473662925048	19.653066754370162	20.024928178848334	33.427268437531026
10	18.674641816858472	30.58479143833324	28.182657518587718	22.55790922622057
11	29.24424218069089	27.788739046704485	21.508945300064195	21.458073472540434
12	25.093966508012905	30.105200478192295	25.78499168721433	19.01584132658047
13	27.00615604837689	24.185353599003886	23.077066586718665	25.73142376590056
14	22.83085190949325	26.110756753491604	25.014771907270127	26.043619429745018
15	26.915083753255338	27.428201197774165	22.209287048693383	23.447428000277114
16	20.84522053811548	29.009341258933745	26.895272361523226	23.25016584142755
17	24.61914987111414	29.015812338322032	24.37678210156673	21.9882556889971
18	23.521310162746616	26.954305883304176	26.36021781273087	23.164166141218338
19	24.50844580342683	27.85115387228478	27.234147875140362	20.406252449148027
20	25.14444153295518	27.624373886697732	25.573713017039516	21.657471563307563
21	22.987231692522368	27.244425482250094	27.537706165494303	22.230636659733232
22	24.09843502815426	27.957044929937847	23.20632698592913	24.738193055978762
23	22.030902565959796	29.75824004934558	24.845625458896688	23.36523192579794
24	22.582315641146078	30.792429935807107	24.800075674547255	21.825178748499557
25	22.753076412343304	27.084979681503143	24.226390861679878	25.93555304447368
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	103787.0
1	103787.0
2	75101.0
3	46415.0
4	46415.0
5	46415.0
6	45247.0
7	44079.0
8	44079.0
9	44079.0
10	43430.5
11	42782.0
12	42782.0
13	42782.0
14	51987.0
15	61192.0
16	61192.0
17	61192.0
18	83457.0
19	105722.0
20	105722.0
21	105722.0
22	145252.5
23	184783.0
24	184783.0
25	184783.0
26	233946.5
27	283110.0
28	283110.0
29	283110.0
30	336705.5
31	390301.0
32	390301.0
33	390301.0
34	423835.0
35	457369.0
36	457369.0
37	457369.0
38	496384.0
39	535399.0
40	535399.0
41	535399.0
42	599391.0
43	663383.0
44	663383.0
45	663383.0
46	1012131.5
47	1360880.0
48	1360880.0
49	1360880.0
50	1224603.0
51	1088326.0
52	1088326.0
53	1088326.0
54	945703.5
55	803081.0
56	803081.0
57	803081.0
58	706105.5
59	609130.0
60	609130.0
61	609130.0
62	528490.5
63	447851.0
64	447851.0
65	447851.0
66	353722.5
67	259594.0
68	259594.0
69	259594.0
70	195633.5
71	131673.0
72	131673.0
73	131673.0
74	95986.5
75	60300.0
76	60300.0
77	60300.0
78	40968.0
79	21636.0
80	21636.0
81	21636.0
82	15213.0
83	8790.0
84	8790.0
85	8790.0
86	5787.5
87	2785.0
88	2785.0
89	2785.0
90	1858.0
91	931.0
92	931.0
93	931.0
94	587.0
95	243.0
96	243.0
97	243.0
98	210.0
99	177.0
100	177.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.653744322291232
2	0.7085039006476642
3	0.6961103975916156
4	0.6809426166548198
5	0.6702084947610873
6	0.5861115760115192
7	0.6011367538796785
8	0.6081502320735303
9	0.6287239657031841
10	0.6297351510989705
11	0.6398858968028263
12	0.6354911295057547
13	0.6318223414671963
14	0.6330539134236028
15	0.6352577790298038
16	0.625690409515825
17	0.6074631445610088
18	0.5919842296562786
19	0.586435673894784
20	0.6064001035039
21	0.6093299483686144
22	0.614269200109571
23	0.6299814654902518
24	0.6393673401896024
25	0.6355948408283993
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	7713719.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.21576203582049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.83572383307319	23.78674108748845
2	14.451351806480414	9.311226217869951
3	4.479882212240652	4.329684578941497
4	1.9494120530963093	2.512071792492438
5	1.068391002819515	1.7209515154022554
6	0.647810464823884	1.2521824667448305
7	0.49501559507493453	1.1163113230467914
8	0.3756676804691873	0.9681936478835189
9	0.27736103938226625	0.8041857518472232
>10	1.9554150264555055	12.424426614621968
>50	0.2409446634176904	5.424979718406174
>100	0.19549724722071632	12.529950835553866
>500	0.01811014185869982	3.909367217888603
>1k	0.007485493364180566	4.344838614896223
>5k	6.036688196702519E-4	1.4398522228166049
>10k+	0.001328071403274554	14.12503639409951
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	179271	2.324054065231051	No Hit
CCCATGTACTCTGCGTTGATACCAC	113596	1.4726489258942412	No Hit
GAGTACATGGGAAGCAGTGGTATCA	76536	0.9922062237423998	No Hit
GCTTCCCATGTACTCTGCGTTGATA	76452	0.9911172548546298	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	55280	0.7166452394752778	No Hit
GCGTTGATACCACTGCTTCCCATGT	47097	0.6105615203250209	No Hit
GTATCAACGCAGAGTACATGGGAAG	45838	0.5942399509238022	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNN	40796	0.5288758898269433	No Hit
CATGTACTCTGCGTTGATACCACTG	34658	0.44930337752775285	No Hit
TATCAACGCAGAGTACATGGGAAGC	32173	0.4170880479312249	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	31461	0.4078577402158414	No Hit
GGTATCAACGCAGAGTACATGGGAA	23398	0.3033296909052559	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	23110	0.29959608329004467	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	22829	0.2959532230821475	No Hit
GTATCAACGCAGAGTACTTTTTTTT	22215	0.28799337906916234	No Hit
ACTCTGCGTTGATACCACTGCTTCC	20425	0.2647879706273978	No Hit
GTGGTATCAACGCAGAGTACATGGG	20166	0.26143031655677373	No Hit
CATGGGAAGCAGTGGTATCAACGCA	17745	0.23004467754140384	No Hit
CAGTGGTATCAACGCAGAGTACATG	17381	0.22532581236106736	No Hit
CCACTGCTTCCCATGTACTCTGCGT	16950	0.219738364853581	No Hit
GCAGTGGTATCAACGCAGAGTACAT	16114	0.20890053163720382	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	15415	0.19983875482111807	No Hit
TATCAACGCAGAGTACTTTTTTTTT	15384	0.19943687344586963	No Hit
GATACCACTGCTTCCCATGTACTCT	14870	0.19277342096594394	No Hit
GGTATCAACGCAGAGTACTTTTTTT	14329	0.18575994277209215	No Hit
GTACTCTGCGTTGATACCACTGCTT	13714	0.1777871348437764	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	13621	0.1765814907180311	No Hit
ATACCACTGCTTCCCATGTACTCTG	13313	0.1725886047962079	No Hit
ACATGGGAAGCAGTGGTATCAACGC	11408	0.1478923460914249	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	11378	0.14750342863150706	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	11335	0.14694598027229147	No Hit
TACCACTGCTTCCCATGTACTCTGC	11093	0.14380871276228757	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	10115	0.1311300035689659	No Hit
TGGGAAGCAGTGGTATCAACGCAGA	9750	0.12639817447329882	No Hit
GCTTTCCATGTACTCTGCGTTGATA	9264	0.12009771162262976	No Hit
CCATGTACTCTGCGTTGATACCACT	9186	0.11908652622684338	No Hit
CTTCCCATGTACTCTGCGTTGATAC	8776	0.11377132094129952	No Hit
ATGGAAAGCAGTGGTATCAACGCAG	8518	0.11042663078600608	No Hit
GTTGATACCACTGCTTCCCATGTAC	8395	0.10883206920034293	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	8272	0.10723750761467976	No Hit
GGTATCAACGCAGAGTACATGGGGG	8140	0.10552627079104125	No Hit
ATCAACGCAGAGTACATGGGAAGCA	7921	0.10268717333364102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	1.296391533059475E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACG	1490	0.0	16.332022	2
ATAACGC	1600	0.0	15.624358	3
CGCACGA	665	0.0	15.425816	12
GTATAAC	1735	0.0	15.340386	1
ACGATTT	675	0.0	15.337299	15
ACGCACG	485	0.0	15.079485	11
CACGATT	700	0.0	14.790119	14
TAACGCA	1890	0.0	14.584778	4
TGTCACG	530	0.0	14.158495	7
TGCGCGT	235	0.0	14.14604	10
TATCACG	755	0.0	14.101241	2
GTCACGC	545	0.0	13.768273	8
CGATTTC	780	0.0	13.758296	16
CGCGGAA	140	0.0	13.568916	14
TGTCGCG	225	0.0	13.5093155	7
TCGAATA	205	0.0	12.972869	18
GGCTACG	525	0.0	12.674083	1
AACGAGC	275	0.0	12.443318	3
TACAACG	565	0.0	12.281762	2
TACGCCC	485	0.0	12.151042	4
>>END_MODULE