FastQCFastQC Report
Wed 25 May 2016
SRR1295356_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295356_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7713719
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCANTGGTATCAAC918121.1902429943325652No Hit
CCCATGTACTCTGCNTTGATACCAC632130.8194879797928859No Hit
GTACTTTTTTTTTTNTTTTTTTTTT460330.596767914413268No Hit
GCTTCCCATGTACTNTGCGTTGATA408920.5301204256986805No Hit
GAGTACATGGGAAGNAGTGGTATCA392520.508859604556505No Hit
GTATCAACGCAGAGNACTTTTTTTT376830.4885192214028019No Hit
TATCAACGCAGAGTNCTTTTTTTTT285960.3707161227936875No Hit
GGTATCAACGCAGANTACTTTTTTT251330.32582208400383783No Hit
GTATCAACGCAGAGNACATGGGAAG241680.3133119057098139No Hit
GCGTTGATACCACTNCTTCCCATGT232100.30089247482310416No Hit
ACGCAGAGTACATGNGAAGCAGTGG184790.23956019139406035No Hit
CATGTACTCTGCGTNGATACCACTG184250.23886013996620828No Hit
TATCAACGCAGAGTNCATGGGAAGC178710.23167813087305875No Hit
GAGTACTTTTTTTTNTTTTTTTTTT140490.18213004647952563No Hit
GGGAAGCAGTGGTANCAACGCAGAG126620.16414909591599072No Hit
GGTATCAACGCAGANTACATGGGAA119440.1548410047086237No Hit
CTGCTTCCCATGTANTCTGCGTTGA115780.150096211697626No Hit
ACTCTGCGTTGATANCACTGCTTCC112230.14549402175526488No Hit
ACGCAGAGTACTTTNTTTTTTTTTT105820.13718415202835366No Hit
CATGGGAAGCAGTGNTATCAACGCA105500.1367693067377746No Hit
GTGGTATCAACGCANAGTACATGGG97910.1269296950018532No Hit
CAGTGGTATCAACGNAGAGTACATG96410.12498510770226398No Hit
CCACTGCTTCCCATNTACTCTGCGT91840.11906059839618217No Hit
GCAGTGGTATCAACNCAGAGTACAT88420.11462693935311878No Hit
ATGGGAAGCAGTGGNATCAACGCAG86790.11251382115423184No Hit
GATACCACTGCTTCNCATGTACTCT78300.10150745703855688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTT4200.022.1007915
CGCACGA4100.021.88517212
CACGATT4400.020.74460414
ACGCACG3750.018.9772111
TCGCGGA1157.2759576E-1118.83371513
GTTCGCG1201.3460522E-1018.04897711
CGCGGAA1304.1836756E-1016.66059514
TGCGCGT1800.016.33002910
CGCGTGG1750.015.91256812
CGTCGTA1001.8691499E-615.47055310
CGCGCAC1401.198714E-915.470551510
CCGCTAG704.7783053E-415.470551513
TCGCGCA1307.516064E-915.4697529
CACGCAC4650.015.30420110
CGAATTC2250.015.12676115
CGCGAGG1351.2361852E-814.89756814
TTGCGAA1154.90556E-714.79791912
CTGCGCG2600.013.684789
GCGCACG1701.2587407E-913.65048711
ATAACGC22750.013.633813
Produced by FastQC (version 0.11.5)