FastQCFastQC Report
Wed 25 May 2016
SRR1295355_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295355_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3900670
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG59490.152512260714185No Hit
GTATCAACGCAGAGTACTTTTTTTT55080.14120651067637097No Hit
GGTATCAACGCAGAGTACTTTTTTT42940.11008365229563126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG2151.8189894E-1210.1705071
CGCAGAT1451.6219383E-79.8264718
GGTATCA36550.09.6762881
GTCCTAT3300.09.5072141
TAACCCG1002.7515003E-49.5019695
CGTAGGC1201.7060249E-59.49807118
GTATTAG6000.09.1903071
GTGGTAT18450.09.1207421
GACTGTC2651.8189894E-128.9611337
TGGTATC18650.08.8646542
GTATTAT8500.08.6124171
ATACCGT1000.002909768.5489226
GTGTTAC3350.08.5139231
ACGTAGG1252.7522258E-48.35798117
TCCGTTG1606.465234E-68.3115578
AAACGTA1502.595895E-58.23134415
CTTGCTC7000.08.1445453
TAGACTG5150.08.1181875
TATACTG9600.08.1162655
CCAGGAC4950.08.0622773
Produced by FastQC (version 0.11.5)