FastQCFastQC Report
Wed 25 May 2016
SRR1295355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295355_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3900670
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT163100.418133295049312No Hit
GGTATCAACGCAGAGTACTTTTTTT132210.33894177154181204No Hit
TATCAACGCAGAGTACTTTTTTTTT92840.23801039308631594No Hit
GTGGTATCAACGCAGAGTACATGGG64910.16640730951349383No Hit
ACGCAGAGTACTTTTTTTTTTTTTT59420.15233280436437843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47600.011.757331
TATACCG855.292608E-511.1836645
GTGGTAT18500.010.3410021
TGGTATC18800.010.0117582
ACGGATC951.6509907E-49.9965598
CAGCGTC3950.09.6264452
AGCGTCA4000.09.5061153
GTACTAG2950.09.033881
TAGACAG5600.08.9968585
GTACCGT850.00745841448.9383296
GCTAGAC2252.582965E-108.8723733
CCTATAC3700.08.7353483
GTCTTGC6150.08.666651
GCAGCGT4000.08.5660531
CCCCTAG2451.3478711E-98.1581471
ACAGCGC1050.0045290248.1400558
CTAGACA4950.08.0657944
TTAGACA4600.08.0595324
TATACTG9200.08.0595325
GTATTAA7350.08.0286521
Produced by FastQC (version 0.11.5)