FastQCFastQC Report
Wed 25 May 2016
SRR1295354_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295354_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3627336
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG50010.1378697755046679No Hit
GTATCAACGCAGAGTACTTTTTTTT44800.1235066175286767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1002.3933419E-510.4529455
GCGTCAG1606.5616405E-89.5076621
CGTCAGA1606.622031E-89.5020212
GGTATCA36250.09.2322671
TTACACC3600.08.974754
ATACCGT1356.8130495E-58.4433296
GTATAGA5800.08.3601861
GTCTTAG3550.08.3024651
CCAGGAC5200.08.223133
TAGGGTG2909.094947E-128.1919635
GTCTAGG3600.08.1871531
GTCCAGG7800.08.1668381
CGTGTAC1050.00450509468.14481453
TTAGACA4750.08.00225454
CAGGACT4750.08.00225454
GTAGGAC2502.064553E-97.9819193
CCTATAC2502.064553E-97.9819193
CTTAGAC2502.064553E-97.9819193
CTTACAC4300.07.95543
GTTCTAG4200.07.92305141
Produced by FastQC (version 0.11.5)