##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295354_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3627336
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.266825846847382	31.0	30.0	33.0	26.0	34.0
2	30.131935668490595	31.0	30.0	34.0	26.0	34.0
3	30.565937922486366	31.0	30.0	34.0	26.0	34.0
4	33.93992864184625	35.0	35.0	37.0	28.0	37.0
5	34.25154962209181	37.0	35.0	37.0	30.0	37.0
6	34.35248209705415	37.0	35.0	37.0	30.0	37.0
7	34.32681091577951	37.0	35.0	37.0	30.0	37.0
8	34.278072668206086	37.0	35.0	37.0	30.0	37.0
9	35.339375508637744	39.0	35.0	39.0	28.0	39.0
10	35.27975268902578	39.0	35.0	39.0	28.0	39.0
11	35.63208757060278	39.0	35.0	39.0	29.0	39.0
12	35.63676455668843	39.0	35.0	39.0	29.0	39.0
13	35.545982506169814	39.0	35.0	39.0	29.0	39.0
14	35.80527251955705	39.0	35.0	41.0	26.0	41.0
15	36.405912493356006	39.0	36.0	41.0	27.0	41.0
16	36.51186325170869	39.0	36.0	41.0	28.0	41.0
17	36.33881807475238	39.0	36.0	41.0	27.0	41.0
18	36.32594361261267	39.0	36.0	41.0	27.0	41.0
19	36.39260989332116	39.0	36.0	41.0	27.0	41.0
20	36.35717341872934	39.0	36.0	41.0	27.0	41.0
21	36.354954434880035	39.0	36.0	41.0	27.0	41.0
22	36.116033364430535	39.0	35.0	41.0	27.0	41.0
23	35.81178418541872	39.0	34.0	41.0	26.0	41.0
24	36.122495131413245	39.0	35.0	41.0	27.0	41.0
25	34.90150650504943	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3924215490226679
1101	2	0.6343993432022756
1101	3	0.42685085586607485
1101	4	0.4646470014141286
1101	5	0.324589992428443
1101	6	0.36147658333812416
1101	7	0.35022777508982017
1101	8	0.3347006060948985
1101	9	0.3487947845865449
1101	10	0.6021696589963952
1101	11	0.5174974051954848
1101	12	0.4873676477002533
1101	13	0.5130405617736855
1101	14	0.9949153119523828
1101	15	0.7046486663042799
1101	16	0.5774405286879798
1101	17	0.8470560972553187
1101	18	0.8625104969987234
1101	19	0.8290226020691094
1101	20	0.8764314111801923
1101	21	0.9276788811243506
1101	22	0.6623977466611137
1101	23	-1.2267072210630303
1101	24	0.19361884190838197
1101	25	0.6386100349266712
1102	1	0.3189935093530494
1102	2	0.3481038952063855
1102	3	0.30407311745783616
1102	4	0.5305251026289142
1102	5	0.2974173098860007
1102	6	0.2730754863440197
1102	7	0.2327735208710422
1102	8	0.2539826440684365
1102	9	0.3880967898458252
1102	10	0.9148097267268653
1102	11	0.492363526445196
1102	12	0.45353710886268317
1102	13	0.3951086319125068
1102	14	0.9957555659454087
1102	15	0.6886674917448588
1102	16	0.49225202780144883
1102	17	0.7219861659601463
1102	18	0.7392327949608557
1102	19	0.7788584071235505
1102	20	0.8165564370981784
1102	21	0.8165552905464892
1102	22	0.7364758890946064
1102	23	0.7864644792450051
1102	24	0.8875740177963962
1102	25	0.6611143375554036
1103	1	-0.28154876233408643
1103	2	-1.556561418671226
1103	3	-0.36520593174316573
1103	4	0.006572643870541128
1103	5	-0.073849175414189
1103	6	-0.05587109941364332
1103	7	0.05223669754086302
1103	8	0.12342965198334355
1103	9	0.7125995998249479
1103	10	0.7954010336266251
1103	11	0.47180527911110204
1103	12	0.3487778179318539
1103	13	0.20836351691414023
1103	14	-0.9197815967768506
1103	15	-0.15536185885633103
1103	16	-0.07809913869655816
1103	17	0.4322247046637955
1103	18	0.6199985771607857
1103	19	0.48033533424247565
1103	20	0.4932728875129939
1103	21	0.6211533847711621
1103	22	0.10119039577126188
1103	23	0.910453286216935
1103	24	0.7778064493801509
1103	25	0.766218800887394
1104	1	-0.08620033566035801
1104	2	0.01711590801815177
1104	3	-0.3162031273186727
1104	4	-0.23683735978042364
1104	5	-0.178793026743584
1104	6	-0.15600048809724854
1104	7	-0.13590808939910914
1104	8	0.0320928763092212
1104	9	-1.955519229137252
1104	10	-0.5576134217974698
1104	11	-0.47527675532032987
1104	12	-0.05950546505022203
1104	13	0.07896941703810967
1104	14	-1.5982091932767304
1104	15	-0.6448316260369396
1104	16	-0.2996213915441146
1104	17	0.12956391164155434
1104	18	0.21578030547263438
1104	19	0.24846519634824205
1104	20	0.4375372576809795
1104	21	0.5158305951110194
1104	22	0.5255840743818112
1104	23	0.9371653291940376
1104	24	0.6932462304825862
1104	25	0.4011908569437921
1105	1	-0.1400027321157431
1105	2	-0.019474532199993178
1105	3	-1.4216459561400292
1105	4	-0.44233387865180873
1105	5	-0.31471750900372086
1105	6	-0.11250691680961467
1105	7	-0.09992944730100817
1105	8	-0.19516462251295508
1105	9	0.23315478769725928
1105	10	0.4377081614684215
1105	11	-0.16686332314361607
1105	12	-0.11535774761558315
1105	13	-0.10640212481932565
1105	14	0.6004711052936358
1105	15	0.2303095452383488
1105	16	-0.2942368164270377
1105	17	0.36259644646961675
1105	18	0.3367178142811582
1105	19	0.20345105573132116
1105	20	-0.314454106274475
1105	21	0.14019527403780785
1105	22	0.28232908825397374
1105	23	0.41178624388457763
1105	24	0.3468999726119577
1105	25	0.4608459386399133
1106	1	-1.6655589549624352
1106	2	-1.2481300872359213
1106	3	-1.263046375615879
1106	4	-1.4824754857901183
1106	5	-0.993910017991432
1106	6	-0.8622747873036971
1106	7	-0.680265347649744
1106	8	-0.823792191772938
1106	9	-0.4680743369266125
1106	10	-2.042031601894159
1106	11	-1.4095638602455622
1106	12	-1.0994502537026065
1106	13	-1.1693141787414447
1106	14	-0.39589584964674884
1106	15	-1.0626001526099884
1106	16	-1.4317278553549357
1106	17	-0.8119829233220841
1106	18	-0.7719946417126522
1106	19	-0.7831469986726773
1106	20	-0.489298682361742
1106	21	-0.4212182607181276
1106	22	-0.40074295077397437
1106	23	-0.6629635268718133
1106	24	-1.0145284799981056
1106	25	-1.8603703874122814
1107	1	0.04481099202876848
1107	2	0.17072221850616387
1107	3	0.019101299899972446
1107	4	0.18357044475464335
1107	5	-0.07231001248239721
1107	6	0.07208600617838101
1107	7	0.0148163663174401
1107	8	-0.0865521278341177
1107	9	0.5094783531129181
1107	10	0.13376193981871154
1107	11	0.11152255599120053
1107	12	-0.01918531762864717
1107	13	0.15943641298234468
1107	14	-0.945452024857758
1107	15	-0.4449896007159211
1107	16	-0.07823657140731655
1107	17	0.13060357626005725
1107	18	0.42191359914328075
1107	19	0.30079109743491017
1107	20	0.3352758728479657
1107	21	0.31490129063100625
1107	22	-0.2697201467969066
1107	23	0.6501959061638019
1107	24	0.42272008176167475
1107	25	0.14870247405463743
1108	1	-0.012796023015539504
1108	2	0.33513680831753234
1108	3	0.0036468937971712023
1108	4	-0.2466218277927439
1108	5	-0.14953642976546888
1108	6	-0.04222474963796685
1108	7	0.021860348116163664
1108	8	-0.07179256428508296
1108	9	0.4810053535898575
1108	10	0.5182018565494602
1108	11	0.3391503787270622
1108	12	0.09572192815185332
1108	13	0.02222462801483971
1108	14	0.21813797876664154
1108	15	0.031449767333064926
1108	16	0.018189227532332097
1108	17	0.38990021121836094
1108	18	0.3574419281469119
1108	19	0.39995777948639955
1108	20	0.47571564332772454
1108	21	0.4604069561430748
1108	22	0.44695644132379897
1108	23	0.39272061323033824
1108	24	0.42025101381648255
1108	25	-0.4991350288251297
1109	1	-0.7598189603725665
1109	2	-0.7394407897361077
1109	3	-0.7948853390951847
1109	4	-0.705033845976196
1109	5	-0.963182839154193
1109	6	-1.1697844273562694
1109	7	-0.8397292234461275
1109	8	-1.2786073234551623
1109	9	-0.9250753870942745
1109	10	-1.0797444519959072
1109	11	-1.1000365718155365
1109	12	-1.1517134721739666
1109	13	-1.2057715919230532
1109	14	-0.7210896354113885
1109	15	-1.3357083303055433
1109	16	-1.339053383001918
1109	17	-1.2687155667383152
1109	18	-1.9411824879189865
1109	19	-1.9730221486242172
1109	20	-1.763473318930167
1109	21	-1.5394154854487923
1109	22	-1.8144200233490722
1109	23	-1.3318157017267538
1109	24	-2.134807793896286
1109	25	-1.0958060448039149
1110	1	0.03665940047371308
1110	2	0.10779145665830114
1110	3	-0.03260074150389869
1110	4	0.1591463025749178
1110	5	0.13658672624679014
1110	6	0.07873645637420879
1110	7	0.020447059876396168
1110	8	-0.06061353232639277
1110	9	0.37160482674786266
1110	10	0.24331205390677013
1110	11	0.1821999862272108
1110	12	0.1920407763190255
1110	13	0.12400653331416578
1110	14	-2.516110908081103
1110	15	-0.5373208477297027
1110	16	-0.266931515048924
1110	17	-0.25155379071508577
1110	18	-2.5195838878561645
1110	19	-0.40726432486800235
1110	20	-1.7903858516580584
1110	21	-0.2613550511011766
1110	22	-1.7651530645854194
1110	23	-0.6075994930044075
1110	24	0.03862371435564427
1110	25	0.020444317347696028
1111	1	-0.18516493496654718
1111	2	-1.325010823775532
1111	3	-0.33232190748527657
1111	4	-0.02052651434917152
1111	5	-0.06632927358811003
1111	6	0.02186242610213185
1111	7	0.022869512162436934
1111	8	0.004058229487213794
1111	9	-1.4254764993080187
1111	10	-0.19730211340759496
1111	11	-0.06098150033335514
1111	12	-0.02035136552037642
1111	13	0.05944429371281501
1111	14	0.7851518237307431
1111	15	0.2569102557647227
1111	16	0.18119335306226247
1111	17	-0.1679742746242141
1111	18	0.0019985278992251665
1111	19	-0.11281269456243592
1111	20	0.2844801667502779
1111	21	-0.478537888668221
1111	22	-0.10952633186438021
1111	23	0.12956702086985672
1111	24	-0.5578758185472381
1111	25	-1.9479113787084046
1112	1	0.19433835904671426
1112	2	0.4237619655882341
1112	3	-0.003346134892893815
1112	4	0.2806506621223832
1112	5	0.04796231921093863
1112	6	0.061405548713310054
1112	7	0.020780088952314202
1112	8	0.026157606924257948
1112	9	-0.31525477080298003
1112	10	0.37833478468461834
1112	11	0.001359373779862949
1112	12	0.054905483344299455
1112	13	0.09685307342279259
1112	14	0.464549642685391
1112	15	0.1635293490591465
1112	16	0.18092444865265378
1112	17	-3.5968533398270353
1112	18	-0.9713807538650201
1112	19	-1.0503133009928831
1112	20	-0.12871497058356596
1112	21	-0.531435690964301
1112	22	-1.6026402338219654
1112	23	-1.4424279044032957
1112	24	-0.35334649916969596
1112	25	0.030775440737819793
1113	1	-0.22831092525698082
1113	2	-0.017364509143831697
1113	3	-0.0988668323287456
1113	4	-0.49735877505044357
1113	5	-0.22818413842519902
1113	6	-0.20978385556776402
1113	7	-0.3717324140502498
1113	8	-0.22717336954920597
1113	9	0.24238965899509424
1113	10	0.3114448941882202
1113	11	0.010818620439849269
1113	12	-0.09672690283031926
1113	13	-0.4195264074353702
1113	14	-1.468658669312802
1113	15	-1.5320560403563235
1113	16	-0.3649581032495419
1113	17	-0.2879520919079681
1113	18	-0.5261686208338361
1113	19	-0.11304070929173804
1113	20	0.3050573539720247
1113	21	-1.5971489765277695
1113	22	-1.8380087573674473
1113	23	-0.7152797117984875
1113	24	-2.307941315677027
1113	25	-0.4836113985288577
1114	1	0.20695065959098713
1114	2	0.3510070266233889
1114	3	-2.962026602908452E-4
1114	4	0.3877167325683004
1114	5	0.1505176275962441
1114	6	0.22230551162065382
1114	7	0.2401379182688359
1114	8	0.19822056425721257
1114	9	0.5072311865303121
1114	10	0.308885695576798
1114	11	0.2627832204876057
1114	12	0.31219879335181844
1114	13	0.23720369540074415
1114	14	1.1122408389935998
1114	15	0.5350686564887823
1114	16	0.3618491099497234
1114	17	-0.22370821490945048
1114	18	0.06121608323046246
1114	19	-0.40611142112219056
1114	20	-1.6317394593787
1114	21	-0.6947647605251746
1114	22	-1.3821005397205397
1114	23	-2.9179966422456403
1114	24	-2.003461981569856
1114	25	-1.2605876018591715
1115	1	0.41758497034422604
1115	2	0.49564687247160677
1115	3	0.36076132371003666
1115	4	0.6012547236909214
1115	5	0.44378285712743093
1115	6	0.402113689874497
1115	7	0.4933825934192342
1115	8	0.5144285598373486
1115	9	0.7622309295039287
1115	10	1.003426123839624
1115	11	0.5966629258442637
1115	12	0.5829702109796102
1115	13	0.4895401370324777
1115	14	1.1133255676452407
1115	15	0.7027291217059854
1115	16	0.6526104441921987
1115	17	0.6061016391233025
1115	18	0.7609557328938337
1115	19	0.1596269836610773
1115	20	0.7637382897283231
1115	21	-0.28495090229187525
1115	22	0.7758608926281525
1115	23	0.8932774129808507
1115	24	0.8388947908214348
1115	25	1.006083375267906
1116	1	0.4655480057547621
1116	2	0.5533427799905617
1116	3	0.360871475403858
1116	4	0.6533679582942966
1116	5	0.4450833829456826
1116	6	0.3999593211947712
1116	7	0.364510240167661
1116	8	0.4295203898929074
1116	9	0.5038124915374453
1116	10	0.4553260677492261
1116	11	0.4750356664663471
1116	12	0.5094719392351621
1116	13	-0.06329783551187518
1116	14	0.853373813641177
1116	15	0.6368170461704636
1116	16	0.6058394552496225
1116	17	0.7909793278196773
1116	18	0.7165311534129799
1116	19	0.8976759429672683
1116	20	0.7305188021098488
1116	21	0.14448043735897897
1116	22	0.18062566691919812
1116	23	-0.6732199827900587
1116	24	-0.3041876326362356
1116	25	-0.8894490035883607
1201	1	0.5220551718161097
1201	2	0.6564215976071246
1201	3	0.53589570633115
1201	4	0.4560450764801942
1201	5	0.48893492519116677
1201	6	0.36445564924982676
1201	7	0.460955528569329
1201	8	0.3552009297288876
1201	9	0.7092148917937493
1201	10	0.9879374036754527
1201	11	0.6442118769161809
1201	12	0.62022634704703
1201	13	0.6303575709050193
1201	14	1.1747503424660906
1201	15	0.8280918503922052
1201	16	0.5981181564433129
1201	17	0.6666021448908381
1201	18	0.7592946876909821
1201	19	0.9052631552354384
1201	20	0.9914808235579784
1201	21	0.9213640135339602
1201	22	1.0932995741794613
1201	23	0.5610659092560937
1201	24	0.9607108716016128
1201	25	1.0962760243731537
1202	1	0.4302499162972673
1202	2	0.7032429732992931
1202	3	0.5520803515388231
1202	4	0.5673389330932181
1202	5	0.40564474092681024
1202	6	0.3271360572482891
1202	7	0.3033998392104067
1202	8	0.3789054381919783
1202	9	0.8178302547418212
1202	10	0.10466766539920513
1202	11	0.365888551146206
1202	12	0.3569307910697006
1202	13	0.3475463853573899
1202	14	1.0765455052561776
1202	15	0.787554579665418
1202	16	0.6247641749064954
1202	17	-1.2941870249403777
1202	18	0.1701213261011958
1202	19	0.557756023996987
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2210	19	0.6408312534755538
2210	20	0.7374923399653497
2210	21	0.8809969536540692
2210	22	0.997313371953993
2210	23	1.3151214508862594
2210	24	1.0666046337314512
2210	25	1.0420587374231687
2211	1	0.0980516517273955
2211	2	-0.5931137428731148
2211	3	-0.07185490719512444
2211	4	0.29645002822784505
2211	5	0.32011148018987257
2211	6	0.2823660041286544
2211	7	0.30811573653375035
2211	8	0.32652528745423837
2211	9	0.7712831781958229
2211	10	0.7916259558705789
2211	11	0.4376921962426934
2211	12	0.3624074256428216
2211	13	0.2670043203383443
2211	14	-0.7524641847818998
2211	15	0.08371279072250104
2211	16	0.2612142579889891
2211	17	0.3846722996927383
2211	18	0.28741525219372477
2211	19	0.5481197228463301
2211	20	0.6435063632365825
2211	21	0.6320304875686631
2211	22	0.04142536610180514
2211	23	0.5461033358232967
2211	24	-0.19035911432193586
2211	25	0.39037780140296263
2212	1	-0.06051554847114815
2212	2	-1.133188802021074
2212	3	-0.42844825682044174
2212	4	0.04257040060078765
2212	5	0.061365487308734146
2212	6	0.08145939715024753
2212	7	-0.006952531682486551
2212	8	0.043198584760062886
2212	9	-2.0790418716864494
2212	10	-0.05944492362896625
2212	11	0.02869419997806233
2212	12	-0.09693131353801476
2212	13	-0.05672866069537008
2212	14	0.5494098543423149
2212	15	0.24637554437376963
2212	16	0.23340885254808086
2212	17	0.22853470486013805
2212	18	0.07793142868570158
2212	19	-0.5718320580198082
2212	20	0.2635624124671949
2212	21	0.11236857041005521
2212	22	-1.7513681769067233
2212	23	-0.47655796815757157
2212	24	0.12065230523379
2212	25	-0.45385792907844547
2213	1	0.11197785430887564
2213	2	0.3876918494896131
2213	3	0.135207526703919
2213	4	0.30129018431726706
2213	5	0.37987889834625577
2213	6	0.2026214869426397
2213	7	0.18634913099243988
2213	8	0.2647881660940641
2213	9	0.5925393043690406
2213	10	0.6714547736886374
2213	11	0.0693515019944968
2213	12	0.1130697209578031
2213	13	0.18382302114544302
2213	14	-1.5060384287179076
2213	15	-0.17340553968548278
2213	16	0.03725031883506347
2213	17	-2.05028062193432
2213	18	-0.7382171895696388
2213	19	-0.4043833232913201
2213	20	0.25477139170615715
2213	21	-0.050581723913239784
2213	22	0.30399074234071577
2213	23	0.8025236027612692
2213	24	0.6779845021611521
2213	25	-0.9139789235244962
2214	1	0.2408775430903809
2214	2	0.1630659926299458
2214	3	0.029368649108121758
2214	4	0.35734986563356586
2214	5	0.29527992632565514
2214	6	0.2321658065746206
2214	7	0.20541378575176594
2214	8	0.1895881691204977
2214	9	0.5713044428537017
2214	10	0.5632836489137318
2214	11	-0.0848403639545694
2214	12	-0.24170170746950959
2214	13	-0.07892962320293861
2214	14	0.8293121082222612
2214	15	0.38521274099627334
2214	16	0.3919832863059156
2214	17	0.3385583868070299
2214	18	0.25183718539568645
2214	19	-0.9118315081863813
2214	20	0.018556176803990354
2214	21	0.37470629190493554
2214	22	0.602979912744189
2214	23	1.0322754854566156
2214	24	0.8304468860847223
2214	25	0.9147405662184056
2215	1	-0.194020736118798
2215	2	0.32794873572214556
2215	3	0.15728354279376333
2215	4	0.35678908875399884
2215	5	0.2563924081351061
2215	6	0.2650741427933383
2215	7	0.16235853831537383
2215	8	0.10771854579991924
2215	9	0.33372959293365057
2215	10	0.5616945027251816
2215	11	0.2540373865705021
2215	12	0.1754500021138341
2215	13	0.07259698919328628
2215	14	0.8112996346370025
2215	15	0.3057163263515008
2215	16	0.3579521868883617
2215	17	0.5740285812595971
2215	18	0.4061872060022296
2215	19	-0.3767589659924795
2215	20	-1.5864490491274097
2215	21	-1.8203338879859743
2215	22	-0.2522131794890612
2215	23	0.4782160644165572
2215	24	0.6528588060460763
2215	25	0.09480419656939887
2216	1	-0.023418491724076063
2216	2	-0.24250144307832855
2216	3	-0.23580350060398203
2216	4	-0.001431072200972494
2216	5	0.12572273751277407
2216	6	-0.06581291411822576
2216	7	-0.028545584184001882
2216	8	-0.06573518465232553
2216	9	0.16794881916329274
2216	10	-1.6544127457055353
2216	11	-0.6152617345511402
2216	12	-0.4092843067204939
2216	13	-0.241285432618902
2216	14	-0.621859951268668
2216	15	-0.5437240094938289
2216	16	-0.11584136809736378
2216	17	0.1096482091798947
2216	18	-0.0016023805580545059
2216	19	-1.3813173328673685
2216	20	-1.0327290132982228
2216	21	-0.8723718398016018
2216	22	-0.7120432356041988
2216	23	-4.431381639498952
2216	24	-2.071468334000066
2216	25	-1.3548368323685551
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5.0
3	13.0
4	29.0
5	52.0
6	102.0
7	335.0
8	171.0
9	467.0
10	1189.0
11	2751.0
12	5277.0
13	8433.0
14	12119.0
15	15789.0
16	18361.0
17	20564.0
18	21715.0
19	22729.0
20	23072.0
21	23346.0
22	24759.0
23	26337.0
24	28079.0
25	31104.0
26	34814.0
27	40380.0
28	46908.0
29	57703.0
30	71211.0
31	88088.0
32	117364.0
33	157264.0
34	213292.0
35	296622.0
36	426434.0
37	646772.0
38	1143686.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.11247070249182	21.851064504870386	14.307668325306839	22.72879646733095
2	15.462566095839794	21.807297951332284	40.67069767647065	22.05943827635727
3	17.559971782526897	27.20757878526977	30.670479371626218	24.561970060577114
4	12.368082329037595	18.32405226814965	39.102211045658095	30.20565435715466
5	13.146269480071716	37.859826230864705	35.67204523513998	13.321859053923596
6	29.53989902986723	39.37711095492025	18.21921090647289	12.863779108739632
7	24.83775459809053	33.29304601110011	24.72144253431247	17.14775685649689
8	22.7281364810141	37.30419273086414	22.310714008929835	17.656956779191923
9	23.798906840986678	17.735619674655844	23.01740129548555	35.44807218887193
10	14.664325667636966	30.29719510298447	34.31040720587959	20.728072023498967
11	30.90699465555624	26.457961004118978	24.460461015838646	18.174583324486136
12	20.44935027549113	27.965066036804583	32.06925247627677	19.516331211427516
13	25.509238908924647	25.001530488151264	27.69916063066039	21.790069972263694
14	20.467820450941186	24.104033648210716	28.732758739571125	26.69538716127698
15	22.146580021964553	31.446213045135448	24.84234006887228	21.56486686402772
16	21.43217995484142	31.78274906985902	26.337770507499037	20.447300467800524
17	19.592417404022825	30.7909563233846	29.145889387062553	20.470736885530023
18	19.666792879395878	30.572841245253578	29.92499213353656	19.83537374181398
19	20.50730566146368	29.342262980650318	30.067300488533107	20.083130869352892
20	21.789585110099534	29.055224995471058	29.242669521073445	19.912520373355967
21	22.292387741220203	29.45325947985853	28.148590735522927	20.105762043398336
22	21.037494491943484	29.052344046859428	29.81784670975313	20.09231475144395
23	21.30687904949438	29.304765736310685	29.04796399205522	20.340391222139715
24	21.01017576726355	28.41193451529922	29.38100793672897	21.19688178070826
25	21.62908758112788	28.900568874934727	28.74372255948458	20.72662098445281
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2885.0
1	2885.0
2	3310.5
3	3736.0
4	3736.0
5	3736.0
6	5602.5
7	7469.0
8	7469.0
9	7469.0
10	12256.5
11	17044.0
12	17044.0
13	17044.0
14	28563.5
15	40083.0
16	40083.0
17	40083.0
18	62681.0
19	85279.0
20	85279.0
21	85279.0
22	119642.0
23	154005.0
24	154005.0
25	154005.0
26	197415.0
27	240825.0
28	240825.0
29	240825.0
30	285675.0
31	330525.0
32	330525.0
33	330525.0
34	367557.5
35	404590.0
36	404590.0
37	404590.0
38	428472.5
39	452355.0
40	452355.0
41	452355.0
42	448086.0
43	443817.0
44	443817.0
45	443817.0
46	420646.5
47	397476.0
48	397476.0
49	397476.0
50	371252.5
51	345029.0
52	345029.0
53	345029.0
54	308494.5
55	271960.0
56	271960.0
57	271960.0
58	233533.5
59	195107.0
60	195107.0
61	195107.0
62	159219.0
63	123331.0
64	123331.0
65	123331.0
66	94067.5
67	64804.0
68	64804.0
69	64804.0
70	47028.0
71	29252.0
72	29252.0
73	29252.0
74	20412.5
75	11573.0
76	11573.0
77	11573.0
78	7894.0
79	4215.0
80	4215.0
81	4215.0
82	2825.5
83	1436.0
84	1436.0
85	1436.0
86	924.0
87	412.0
88	412.0
89	412.0
90	253.5
91	95.0
92	95.0
93	95.0
94	59.0
95	23.0
96	23.0
97	23.0
98	16.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07942467970984766
2	0.021613658067518422
3	0.03346808787495837
4	0.041214819911913315
5	0.05061565843362733
6	0.019821709375696103
7	0.02439807065019618
8	0.04171105185734103
9	0.041738620298753686
10	0.026575977521795607
11	0.02627272466625645
12	0.019904414699934057
13	0.02872631595198239
14	0.04372354808046456
15	0.04118725147050067
16	0.02767871517830165
17	0.026989504142985378
18	0.03261346619116619
19	0.021089857680678052
20	0.018277876656587644
21	0.01419774732751529
22	0.02288180637250037
23	0.022137458454358792
24	0.021475815860455168
25	0.11148677707276083
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3627336.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.09889653803354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.10961473881072	71.45592529708806
2	9.211618318159712	14.941041618645782
3	1.5170220831299834	3.69086450897012
4	0.45240731793290245	1.4675893708035883
5	0.2074241452622682	0.8410934648057364
6	0.11313614456363516	0.5505129889606953
7	0.08303958656197803	0.4714093187405656
8	0.04896281171689131	0.31766640013115083
9	0.042365092025530196	0.3092185993502469
>10	0.19281201650275312	2.7678366814148414
>50	0.012752376316844396	0.7080807311499543
>100	0.007650477560180489	1.1237332872751808
>500	7.510746492682152E-4	0.4044788808772957
>1k	4.096770529435941E-4	0.8120863442781215
>5k	3.413975441196612E-5	0.13846250750860464
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	5001	0.1378697755046679	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4480	0.1235066175286767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.7568441412651047E-5	0.0	0.0	0.0
4	0.0	2.7568441412651047E-5	0.0	0.0	0.0
5	0.0	2.7568441412651047E-5	0.0	2.7568441412651047E-5	0.0
6	0.0	2.7568441412651047E-5	0.0	2.7568441412651047E-5	0.0
7	0.0	2.7568441412651047E-5	0.0	2.7568441412651047E-5	0.0
8	0.0	2.7568441412651047E-5	0.0	2.7568441412651047E-5	0.0
9	0.0	2.7568441412651047E-5	0.0	5.5136882825302095E-5	0.0
10	0.0	2.7568441412651047E-5	0.0	5.5136882825302095E-5	0.0
11	0.0	2.7568441412651047E-5	0.0	8.270532423795314E-5	0.0
12	0.0	2.7568441412651047E-5	0.0	1.3784220706325524E-4	0.0
13	0.0	2.7568441412651047E-5	0.0	1.6541064847590628E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	100	2.3933419E-5	10.452945	5
GCGTCAG	160	6.5616405E-8	9.507662	1
CGTCAGA	160	6.622031E-8	9.502021	2
GGTATCA	3625	0.0	9.232267	1
TTACACC	360	0.0	8.97475	4
ATACCGT	135	6.8130495E-5	8.443329	6
GTATAGA	580	0.0	8.360186	1
GTCTTAG	355	0.0	8.302465	1
CCAGGAC	520	0.0	8.22313	3
TAGGGTG	290	9.094947E-12	8.191963	5
GTCTAGG	360	0.0	8.187153	1
GTCCAGG	780	0.0	8.166838	1
CGTGTAC	105	0.0045050946	8.1448145	3
TTAGACA	475	0.0	8.0022545	4
CAGGACT	475	0.0	8.0022545	4
GTAGGAC	250	2.064553E-9	7.981919	3
CCTATAC	250	2.064553E-9	7.981919	3
CTTAGAC	250	2.064553E-9	7.981919	3
CTTACAC	430	0.0	7.9554	3
GTTCTAG	420	0.0	7.9230514	1
>>END_MODULE