FastQCFastQC Report
Wed 25 May 2016
SRR1295354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295354_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3627336
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT131830.36343476314297873No Hit
GGTATCAACGCAGAGTACTTTTTTT110270.3039972034573031No Hit
TATCAACGCAGAGTACTTTTTTTTT78800.21723931833169025No Hit
GTGGTATCAACGCAGAGTACATGGG55250.15231563880489704No Hit
ACGCAGAGTACTTTTTTTTTTTTTT52680.1452305493618457No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGCG500.001504582113.2948769
AGACCGT600.005892681411.0796756
GCAGCGT3250.010.837511
AGCGTCA3600.010.8270663
CAGCGTC3800.010.5073972
GGTATCA45800.010.0806251
CCGTACC1304.2046595E-69.5066934
ACCGAGT900.00111820079.496348
GACTGCG900.00111820079.496347
TATTCCG1554.221729E-79.2000265
CAACGCG950.00181302359.006345
ACTGCGT950.00183108698.9965328
TAGGACT3700.08.9928184
CTAGTAC2252.582965E-108.8729133
TCCGTAC1304.3358497E-58.7754093
CCTATAC3250.08.7754093
CCAGTAC3900.08.7754093
GTTCTAA4350.08.5346651
TAGGACC2803.6379788E-128.4881184
CTTACAC4500.08.4503943
Produced by FastQC (version 0.11.5)