FastQCFastQC Report
Wed 25 May 2016
SRR1295353_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295353_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535958
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA772795.031322471057152No Hit
GCTTATGTACTCTGCGTTGATACCA460072.995329299368863No Hit
CTTATGTACTCTGCGTTGATACCAC299611.9506392752926835No Hit
GAGTACATAAGCAGTGGTATCAACG204191.32939832990225No Hit
TATGTACTCTGCGTTGATACCACTG189691.2349947068865164No Hit
CCCATGTACTCTGCGTTGATACCAC168281.095602874557768No Hit
GCGTTGATACCACTGCTTATGTACT154491.00582177377246No Hit
CATAAGCAGTGGTATCAACGCAGAG136700.8899982942241911No Hit
ACTCTGCGTTGATACCACTGCTTAT125160.8148660314930487No Hit
CTGCTTATGTACTCTGCGTTGATAC114930.748262647806776No Hit
CATGTACTCTGCGTTGATACCACTG112320.7312699956639439No Hit
ACGCAGAGTACATAAGCAGTGGTAT110820.7215041036278336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA103890.6763856824210037No Hit
GTATCAACGCAGAGTACATAAGCAG103840.6760601526864667No Hit
TATCAACGCAGAGTACATAAGCAGT92080.5994955591233615No Hit
GGTATCAACGCAGAGTACATAAGCA85120.5541818200758094No Hit
GTACATGATAAGCAGTGGTATCAAC83760.5453274112964026No Hit
ATCATGTACTCTGCGTTGATACCAC83100.5410304188005141No Hit
AAGCAGTGGTATCAACGCAGAGTAC70660.46003862084770547No Hit
GTATCAACGCAGAGTACTTTTTTTT67720.4408974724569291No Hit
GTACATGGGAAGCAGTGGTATCAAC67240.43777238700537385No Hit
GTGGTATCAACGCAGAGTACATAAG60130.3914820587542107No Hit
ACATAAGCAGTGGTATCAACGCAGA55280.3599056745041206No Hit
GTGGTATCAACGCAGAGTACATGGG52620.34258749262675153No Hit
GTACTCTGCGTTGATACCACTGCTT52210.33991814880354804No Hit
GCAGTGGTATCAACGCAGAGTACAT52100.3392019833875666No Hit
GTACTTTTTTTTTTTTTTTTTTTTT45980.29935714388023626No Hit
CCACTGCTTATGTACTCTGCGTTGA44320.2885495566936075No Hit
GGTATCAACGCAGAGTACTTTTTTT44190.2877031793838113No Hit
GATACCACTGCTTATGTACTCTGCG43430.2827551274188487No Hit
TATCAACGCAGAGTACTTTTTTTTT40930.2664786406919981No Hit
GTACATGGGATAAGCAGTGGTATCA39140.25482467619557303No Hit
ATACCACTGCTTATGTACTCTGCGT38910.2533272394167028No Hit
GTTGATACCACTGCTTATGTACTCT38240.24896514097390685No Hit
GTACTGGTTCACTATCGGTCAGTCA36140.23529289212335233No Hit
GCTTATCCCATGTACTCTGCGTTGA35840.23333971371613027No Hit
GAGTACATGATAAGCAGTGGTATCA32760.2132870820686503No Hit
CAGTGGTATCAACGCAGAGTACATA32070.20879477173203956No Hit
TGATACCACTGCTTATGTACTCTGC29010.18887235197837443No Hit
GAGTACATGGGAAGCAGTGGTATCA28880.18802597466857818No Hit
GATAAGCAGTGGTATCAACGCAGAG28690.18678896167733755No Hit
TACCACTGCTTATGTACTCTGCGTT28080.18281749891598598No Hit
GCTTATCATGTACTCTGCGTTGATA27450.17871582426081964No Hit
GCAGAGTACATAAGCAGTGGTATCA26960.1755256328623569No Hit
GAACAAAAAAAAAAAAAAAAAAAAA26950.17546052691544953No Hit
CCCCATGTACTCTGCGTTGATACCA26750.17415840797730145No Hit
GTATCAACGCAGAGTACATGGGAAG25310.16478315162263552No Hit
GGATAAGCAGTGGTATCAACGCAGA24860.1618533840118024No Hit
GGTATCAACGCAGAGTACATGGGAA24690.16074658291437655No Hit
GGATACCACGTGTCCCGCCCTACTC24220.15768660340972865No Hit
ATACAGGGTGACAGCCCCGTACACA23970.1560589547370436No Hit
GCGTTGATACCACTGCTTATCATGT23830.15514747148033997No Hit
GCGTTGATACCACTGCTTCCCATGT22990.14967857194011813No Hit
ATCAACGCAGAGTACATAAGCAGTG21890.14251691778030387No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21720.14141011668287803No Hit
TATCAACGCAGAGTACATGGGAAGC20570.13392293278852677No Hit
GGGATAAGCAGTGGTATCAACGCAG20450.13314166142563794No Hit
ACGCAGAGTACATGATAAGCAGTGG20450.13314166142563794No Hit
GAGTACATGGGATAAGCAGTGGTAT20200.13151401275295288No Hit
CAGTGGTATCAACGCAGAGTACATG20130.13105827112460106No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA20040.13047231760243444No Hit
GTACACAAGCAGTGGTATCAACGCA19880.129430622451916No Hit
TCCATGTACTCTGCGTTGATACCAC19800.12890977487665678No Hit
TATCAACGCAGAGTACATGATAAGC19160.12474299427458303No Hit
ACGCAGAGTACATGGGAAGCAGTGG18940.12331066344262018No Hit
GTATCAACGCAGAGTACATGGGATA18920.12318045154880537No Hit
AAAAAGTACTCTGCGTTGATACCAC18550.12077153151323149No Hit
ATGTACTCTGCGTTGATACCACTGC18130.11803708174312058No Hit
GTATCAACGCAGAGTACATGATAAG17790.1158234795482689No Hit
GGTATCAACGCAGAGTACATGGGAT17790.1158234795482689No Hit
ACTCTGCGTTGATACCACTGCTTCC17430.1134796654596024No Hit
TACATAAGCAGTGGTATCAACGCAG16090.1047554685740105No Hit
GCGTTGATACCACTGCTTATCCCAT15970.10397419721112167No Hit
ATGATAAGCAGTGGTATCAACGCAG15880.10338824368895505No Hit
GTACATGGTAAGCAGTGGTATCAAC15810.10293250206060323No Hit
GTACATAAGCAGTTGTATCAACGCA15750.10254186637915881No Hit
ACCCTGTATCGCGCGCCTTTCCAGA15720.10234654853843661No Hit
CCCATATTCAGACAGGATACCACGT15370.1000678403966775No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAGC250.00602464519.004042
AACCGTC250.006041668418.99475716
CAGTTGT2450.016.67104110
ACTTCTC402.76622E-416.62095518
AGTTGTA2500.016.33708811
AGACATT350.002172769516.2828126
TGGTTGG508.727093E-515.197296
TATACTG902.2791937E-914.7814015
TGTATCA3050.014.32484914
CGCCAGA400.005286452414.24699714
GTACAAA4750.014.2118151
TTGTATC2900.014.08369613
GTTGTAT2950.013.84498912
ACTGCCT551.9630931E-413.815728
AATCGCC852.7069473E-713.40850118
ATGGTTG655.4398777E-513.1570715
AGACTAT802.0033622E-613.0601726
TACTCTT2400.013.0601728
CGGCGTT1102.752131E-912.9526599
TCGTTGA2200.012.95181614
Produced by FastQC (version 0.11.5)