##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295353_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1535958
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.272715139346257	31.0	30.0	33.0	22.0	34.0
2	29.025618539048594	31.0	28.0	34.0	16.0	34.0
3	29.49888929254576	31.0	30.0	34.0	23.0	34.0
4	32.52296091429584	35.0	33.0	37.0	19.0	37.0
5	33.21932305440644	35.0	33.0	37.0	27.0	37.0
6	33.23538143621115	35.0	33.0	37.0	25.0	37.0
7	33.29412392786782	35.0	33.0	37.0	26.0	37.0
8	33.17398717933693	35.0	33.0	37.0	25.0	37.0
9	34.017866373950326	38.0	33.0	39.0	19.0	39.0
10	33.925596923874224	37.0	33.0	39.0	20.0	39.0
11	34.407397207475725	38.0	34.0	39.0	25.0	39.0
12	34.43234320209277	38.0	34.0	39.0	25.0	39.0
13	34.12008466377336	38.0	33.0	39.0	23.0	39.0
14	34.25557079034713	38.0	32.0	40.0	17.0	41.0
15	34.78915894835666	38.0	33.0	40.0	21.0	41.0
16	34.709635289506615	38.0	33.0	40.0	22.0	41.0
17	34.801656685925	38.0	33.0	40.0	22.0	41.0
18	34.75688853471254	38.0	33.0	40.0	21.0	41.0
19	34.8389571850272	39.0	33.0	40.0	21.0	41.0
20	34.89068776620194	39.0	33.0	40.0	21.0	41.0
21	34.90003893335625	39.0	33.0	40.0	22.0	41.0
22	34.66438275004916	38.0	32.0	40.0	21.0	41.0
23	34.313706494578625	38.0	32.0	40.0	19.0	41.0
24	34.645912843971	38.0	32.0	40.0	22.0	41.0
25	33.41705958105625	38.0	30.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.39042338604385307
1101	2	0.4464627794476712
1101	3	0.2908262361130518
1101	4	0.11093735341476929
1101	5	0.0730957487297772
1101	6	0.10630832174850013
1101	7	-0.039328483466164244
1101	8	0.21946646736875408
1101	9	0.03309228275303866
1101	10	0.3126686378991792
1101	11	0.39464792664494297
1101	12	0.3378144792049653
1101	13	0.24850490635189004
1101	14	1.093696707953967
1101	15	0.5386048334695772
1101	16	0.616168241692435
1101	17	0.7254088283681597
1101	18	0.6263789744267214
1101	19	0.7822430129044307
1101	20	0.6059225303602744
1101	21	0.6645971227840661
1101	22	0.544810202163319
1101	23	-1.4538740981896652
1101	24	-0.07087004321122947
1101	25	0.6614139101830006
1102	1	0.4239014939950039
1102	2	0.34436477439282953
1102	3	0.44438302947795805
1102	4	0.5586140070762582
1102	5	0.3219845830896162
1102	6	0.33959912242575285
1102	7	0.26650176215992616
1102	8	0.30178351560242334
1102	9	0.6738508363155589
1102	10	1.0638223361954147
1102	11	0.8124388459228697
1102	12	0.7312955317246193
1102	13	0.7243610805394738
1102	14	1.384050792315854
1102	15	0.9920045831812558
1102	16	0.8187709130704093
1102	17	1.1653772560503768
1102	18	1.1217600915632957
1102	19	1.040688607911754
1102	20	1.3014042535871937
1102	21	1.1231106064539773
1102	22	0.8684714459163345
1102	23	1.0339075784149188
1102	24	0.8945288231299031
1102	25	0.836780958825635
1103	1	-0.1312089985654552
1103	2	-1.4074688670870863
1103	3	-0.43111525370536796
1103	4	0.0946962788276764
1103	5	-0.05718436927972448
1103	6	0.18697310401822165
1103	7	0.07412686628637744
1103	8	0.3222915172548966
1103	9	0.8659690562441753
1103	10	0.9707999938750405
1103	11	0.7056470920608788
1103	12	0.5465867133143831
1103	13	0.21340257535516116
1103	14	-0.8866993245089532
1103	15	0.041205095178654005
1103	16	0.19598161083617782
1103	17	0.7247550708508967
1103	18	0.6301802815983066
1103	19	0.8062378199369178
1103	20	0.8761199798988528
1103	21	0.9589240118462072
1103	22	0.3210199197441952
1103	23	1.0967782855266748
1103	24	0.8815633016849276
1103	25	0.5564017692172882
1104	1	0.08688265198839318
1104	2	0.0768532793816128
1104	3	-0.25720444933366693
1104	4	-0.009424955852118444
1104	5	0.09350266891227221
1104	6	-0.06357279016141604
1104	7	-0.16332538129172747
1104	8	0.12138576622242425
1104	9	-1.9300512082570052
1104	10	-0.4928698072731521
1104	11	-0.2948604439070479
1104	12	0.10685776380373113
1104	13	0.26832158980484166
1104	14	-1.3941660345535425
1104	15	-0.42199446270743834
1104	16	0.06294964992177654
1104	17	0.26140314562383793
1104	18	0.4470382894432703
1104	19	0.36762161373454205
1104	20	0.5338596015164043
1104	21	0.5672859599319153
1104	22	0.6811655246745048
1104	23	1.0753630379954515
1104	24	0.7795776959590413
1104	25	0.5307149086096032
1105	1	-0.3889858610517791
1105	2	-0.20378177600312952
1105	3	-1.7436622462541287
1105	4	-0.6898776577929411
1105	5	-0.57871180861531
1105	6	-0.17860772029038685
1105	7	-0.21689987507956943
1105	8	-0.17201654397091204
1105	9	0.22734049828825675
1105	10	0.3760759542481935
1105	11	-0.28000355796377363
1105	12	-0.18661241720666055
1105	13	-0.3709583044172362
1105	14	0.5218418542618437
1105	15	0.20926338822238932
1105	16	-0.40178804912106614
1105	17	0.07171462599049505
1105	18	0.03688452792767549
1105	19	0.04774393921137943
1105	20	-0.4802995436991395
1105	21	0.038946841419416955
1105	22	0.08708400356380963
1105	23	0.15514033553034068
1105	24	0.08168255313945139
1105	25	0.43395841545522273
1106	1	-1.629220551963435
1106	2	-1.223452858478172
1106	3	-1.1604265082449743
1106	4	-1.2551740982695208
1106	5	-1.0119268114185118
1106	6	-0.8458107051871266
1106	7	-0.8846391782063918
1106	8	-0.8304383819736216
1106	9	-0.5069940840219971
1106	10	-1.9209458377843838
1106	11	-1.21943243377509
1106	12	-1.0193796368526904
1106	13	-1.0516026889464598
1106	14	-0.4527274189600661
1106	15	-1.0519910856567236
1106	16	-1.5536597510983867
1106	17	-0.9109273919281264
1106	18	-0.7626929846502648
1106	19	-0.6424210060075453
1106	20	-0.31776228417584207
1106	21	-0.24116876495462236
1106	22	-0.5682202567001227
1106	23	-0.9370054603306457
1106	24	-1.1394496012666835
1106	25	-1.687824739287791
1107	1	0.11599400195789045
1107	2	0.2936694983596908
1107	3	0.026364505076088562
1107	4	0.2508637362159405
1107	5	-0.03061526864999564
1107	6	0.11921983651944856
1107	7	-0.07844557281893572
1107	8	-0.15648607391182168
1107	9	0.4836270608893045
1107	10	0.19941134537615568
1107	11	-0.019921834119813298
1107	12	0.05079828920484175
1107	13	0.2998386500467092
1107	14	-0.8906577782748286
1107	15	-0.36409405070520506
1107	16	-0.052104067582426694
1107	17	0.2045188121327186
1107	18	0.33597027916970035
1107	19	0.2231621124776879
1107	20	0.3353632131577271
1107	21	0.5932784991199753
1107	22	-0.06870280891230607
1107	23	0.7843845369590383
1107	24	0.5067994064774908
1107	25	0.12228484047800237
1108	1	-0.015470474975003867
1108	2	0.1305457008339097
1108	3	-0.056914841006392436
1108	4	-0.388601080143097
1108	5	-0.45957027673584605
1108	6	-0.3167957248636526
1108	7	-0.15980846668788473
1108	8	-0.38614147478551786
1108	9	0.22040584153353393
1108	10	0.29064305430518544
1108	11	0.10481090859027375
1108	12	-0.02149131962284656
1108	13	-0.035378117604672354
1108	14	0.19721850742407554
1108	15	-0.061062714165814214
1108	16	-0.013438697697679913
1108	17	0.21515900573616875
1108	18	0.1706436386236163
1108	19	0.305969199303874
1108	20	0.4062929960065915
1108	21	0.26396105571684103
1108	22	0.391487726646659
1108	23	-0.05964507144437192
1108	24	0.18024061640113587
1108	25	-0.6040546280916246
1109	1	-0.507058089017896
1109	2	-0.5596342002290626
1109	3	-0.540692592157523
1109	4	-0.4567837541815436
1109	5	-0.8333721630438546
1109	6	-1.1841947765745076
1109	7	-0.8212654770095185
1109	8	-1.1217711285059266
1109	9	-0.6717870343159049
1109	10	-0.9482326924297197
1109	11	-1.0078619316555262
1109	12	-0.8618891217830296
1109	13	-0.9138974856401418
1109	14	-0.5288330836133994
1109	15	-0.8622020305075395
1109	16	-0.9035942924324516
1109	17	-1.1305759183493507
1109	18	-1.7797899383435691
1109	19	-1.6988585167571344
1109	20	-1.4801406494291243
1109	21	-1.2320393550943436
1109	22	-1.4959342620217981
1109	23	-0.8409928744604116
1109	24	-1.7119464396232402
1109	25	-0.8211358146512495
1110	1	0.35480428368160943
1110	2	0.45384213074789415
1110	3	0.20454755908887634
1110	4	0.48530393065833977
1110	5	0.38783382797480925
1110	6	0.4286876440600693
1110	7	0.14490516838343837
1110	8	0.1533870831871127
1110	9	0.5124852438113408
1110	10	0.4835219561473707
1110	11	0.48770779483768933
1110	12	0.4069491016774691
1110	13	0.3268355575446904
1110	14	-2.2069838627746208
1110	15	-0.31428703306004024
1110	16	0.17398991841264433
1110	17	0.05643869579063221
1110	18	-2.507636061573038
1110	19	-0.09024733594500844
1110	20	-1.6677543654354565
1110	21	0.016235934116807016
1110	22	-1.4575988376579758
1110	23	-0.14581268531588876
1110	24	0.5255439776887627
1110	25	0.37903031411584465
1111	1	-0.15809943063757004
1111	2	-1.2477241877646925
1111	3	-0.3035787479883183
1111	4	0.13982471636157356
1111	5	-0.026573763364609704
1111	6	0.060197040521728695
1111	7	-0.058831525106306515
1111	8	-0.1159648231026651
1111	9	-1.5406051852306888
1111	10	-0.12565544383087257
1111	11	-0.11699474258930564
1111	12	-0.11507660162648392
1111	13	0.05589829041954886
1111	14	0.940811551041044
1111	15	0.4056322194946347
1111	16	0.3439878809007624
1111	17	0.03470479150971073
1111	18	0.16197930516493386
1111	19	-0.04750373540662167
1111	20	0.5145256453401927
1111	21	-0.49389506473549005
1111	22	-0.0470646846773235
1111	23	0.07220924884099134
1111	24	-0.4078373807269031
1111	25	-1.7940000924585675
1112	1	0.33721545977243395
1112	2	0.5341225816315749
1112	3	0.19174800959426008
1112	4	0.43995921655554326
1112	5	0.28976611312653233
1112	6	0.33693632927026584
1112	7	0.2907284117202238
1112	8	0.1915142869128772
1112	9	-0.0908682603894988
1112	10	0.7279569652870563
1112	11	0.2967963493760948
1112	12	0.29415799749958893
1112	13	0.21153425303361217
1112	14	0.8804668113491445
1112	15	0.4097843927500051
1112	16	0.46697056602740616
1112	17	-3.138321258939815
1112	18	-0.6569572256861207
1112	19	-0.8605747072829288
1112	20	0.2440177921969564
1112	21	-0.1043460991935774
1112	22	-1.2846782397295513
1112	23	-1.2060254707953817
1112	24	-0.01725195973227045
1112	25	0.20624510672691088
1113	1	-0.42222082989905374
1113	2	-0.12734868752515993
1113	3	-0.08308954560062531
1113	4	-0.5307690144224431
1113	5	-0.05982824553210264
1113	6	-0.1590656124225518
1113	7	-0.19699264695876906
1113	8	-0.1843970837426241
1113	9	0.3067349095642058
1113	10	0.49746277088664215
1113	11	0.05186350420441954
1113	12	0.08013347238056667
1113	13	-0.4425135293663871
1113	14	-1.36993984061138
1113	15	-1.7115482618384519
1113	16	-0.5503511204090898
1113	17	-0.34489507578595635
1113	18	-0.589568161922081
1113	19	-0.1691933490009916
1113	20	0.2113329014587464
1113	21	-1.6002218764941958
1113	22	-2.106842193780281
1113	23	-0.6175671980380741
1113	24	-2.255454570291967
1113	25	-0.4187199902362977
1114	1	-0.05187433660010399
1114	2	0.19111704320835088
1114	3	-0.015224230731714528
1114	4	0.2166705840334373
1114	5	0.24501827630403739
1114	6	0.10758437512485841
1114	7	0.10467262743922134
1114	8	-0.022964828423035044
1114	9	0.3819605478891219
1114	10	0.2319213556498596
1114	11	0.20506878124859185
1114	12	0.17030416365493295
1114	13	0.09991883102192389
1114	14	0.8745041642451312
1114	15	0.42300520906140093
1114	16	0.4152907586866448
1114	17	-0.40790874509706754
1114	18	-0.14856895188322028
1114	19	-0.4862314098215137
1114	20	-1.5362998094180753
1114	21	-0.5371968571667338
1114	22	-1.4958061188883391
1114	23	-2.794711443125788
1114	24	-1.9501071884488823
1114	25	-1.159956966903259
1115	1	0.20176054341176552
1115	2	0.26770071300652276
1115	3	0.24029962118136794
1115	4	0.4721909587016029
1115	5	0.35178161700250143
1115	6	0.25003302449670173
1115	7	0.23524085562067398
1115	8	0.24496185933229953
1115	9	0.4015837999940146
1115	10	0.645541624609784
1115	11	0.3282120980501091
1115	12	0.31414983756486947
1115	13	0.07281536278473055
1115	14	0.8857707689174887
1115	15	0.47139189988995156
1115	16	0.4473867226981483
1115	17	0.3757546284530875
1115	18	0.6180325743954782
1115	19	0.05129799773543198
1115	20	0.7360618558444472
1115	21	-0.3385362256725699
1115	22	0.6369863869534313
1115	23	0.9473813019504362
1115	24	0.962756200202449
1115	25	0.9847028790647272
1116	1	0.5613221443383125
1116	2	0.808322217066749
1116	3	0.5824611484052795
1116	4	0.9769842622906637
1116	5	0.6764751259060446
1116	6	0.6164160676259058
1116	7	0.5501638297852622
1116	8	0.5084553642874852
1116	9	0.6870925126985057
1116	10	0.5891008696842093
1116	11	0.683443833615577
1116	12	0.6967913861099007
1116	13	0.018401759383884553
1116	14	1.0329771428793961
1116	15	0.7221202069667214
1116	16	0.9156730376979496
1116	17	0.8921488585895645
1116	18	0.8684581742164852
1116	19	1.136946219001274
1116	20	0.8320024528358019
1116	21	0.3902015508768315
1116	22	0.7824013591175287
1116	23	-0.47231408242197404
1116	24	5.2530864060429394E-5
1116	25	-0.5302727398399938
1201	1	0.30653129145267854
1201	2	0.349398542182513
1201	3	0.3001274184210594
1201	4	0.09862070588432204
1201	5	-0.0040196745181972915
1201	6	-0.03939141872844232
1201	7	0.040682687948084606
1201	8	0.04965100802600375
1201	9	0.396891837529715
1201	10	0.6613057743586523
1201	11	0.28323397511647386
1201	12	0.2904086481840409
1201	13	0.19858387387802168
1201	14	0.7623447218431068
1201	15	0.4386466446106141
1201	16	0.32874408563235136
1201	17	0.32666136602676943
1201	18	0.1307719207565725
1201	19	0.42261019965160784
1201	20	0.4434437829959279
1201	21	0.5448611209417606
1201	22	0.31465489663983703
1201	23	0.08626198400842355
1201	24	0.3703590083455026
1201	25	0.6980148277415523
1202	1	0.4902522540339511
1202	2	0.7433062441848648
1202	3	0.7212596363491706
1202	4	0.6302614682399934
1202	5	0.6421339178226617
1202	6	0.36947271117364267
1202	7	0.4746623593692547
1202	8	0.4772578210533496
1202	9	1.1024604745457651
1202	10	-0.07145001581852739
1202	11	0.5362394132587767
1202	12	0.40276495115303845
1202	13	0.40650830598826104
1202	14	1.2638293660703326
1202	15	0.8142939828128632
1202	16	0.8259709921280702
1202	17	-1.0595792055361883
1202	18	0.29832014379319105
1202	19	0.6681959750163173
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2210	18	0.5954610555836481
2210	19	0.8299920228033528
2210	20	0.886767472376107
2210	21	0.8738787715166936
2210	22	1.0107728898852315
2210	23	1.5635324433778521
2210	24	1.1345055678912885
2210	25	1.1184596242058404
2211	1	0.11919195436830776
2211	2	-0.5048460984623766
2211	3	0.02547935605670304
2211	4	0.23362268780242346
2211	5	0.10359913264182552
2211	6	-0.0262561420252041
2211	7	0.1220610302273002
2211	8	0.17506063713913989
2211	9	0.5735203135720539
2211	10	0.6791669118880748
2211	11	0.2598087496814614
2211	12	0.14664338316299563
2211	13	0.07233797998063096
2211	14	-0.9617559255834749
2211	15	-0.2236521710788537
2211	16	0.021954282764518496
2211	17	0.15954348049137224
2211	18	0.22513804055338227
2211	19	0.49017136766201475
2211	20	0.6322188660337176
2211	21	0.5490751497822686
2211	22	-0.0841894423348748
2211	23	0.2927032832571257
2211	24	-0.31115819902068864
2211	25	0.12237881517763327
2212	1	-0.1508799609797684
2212	2	-1.0898670985092132
2212	3	-0.3344130281857751
2212	4	-0.239223034580057
2212	5	-0.05337646482220748
2212	6	0.05021790162665951
2212	7	0.06444728464614968
2212	8	0.01255121234978418
2212	9	-2.368758582353866
2212	10	-0.31811284488474456
2212	11	-0.06440829192086284
2212	12	-0.16501238494002024
2212	13	-0.05082068168938747
2212	14	0.28893291685119493
2212	15	0.14407391997038843
2212	16	0.2583339366974684
2212	17	0.21469754570068744
2212	18	0.02632099591665593
2212	19	-0.5440982548648705
2212	20	0.3115951181371841
2212	21	0.27137401999492283
2212	22	-1.7415453296371481
2212	23	-0.40561143577365755
2212	24	0.1495583224454009
2212	25	-0.6817788444797799
2213	1	0.1828514639151706
2213	2	0.5339311370092865
2213	3	0.18522006181784434
2213	4	0.3432765438630767
2213	5	0.3710830010529733
2213	6	0.28980642938638823
2213	7	0.2470539303468442
2213	8	0.16862253158537754
2213	9	0.6502389799833423
2213	10	0.5700496746992272
2213	11	0.014537930520369002
2213	12	0.12719004093555242
2213	13	0.10632131012177126
2213	14	-1.818914916673954
2213	15	-0.37059724111999515
2213	16	0.04048530098644676
2213	17	-2.231874910510605
2213	18	-0.8536963902343402
2213	19	-0.6129803188072671
2213	20	0.278233026167392
2213	21	-0.09155697073737912
2213	22	0.5911809072434266
2213	23	0.9072438384347592
2213	24	0.8072403981750185
2213	25	-0.9124442822195178
2214	1	0.04957776087402621
2214	2	0.04615848534434619
2214	3	-0.047746074453066
2214	4	0.20576909007655786
2214	5	0.30159801847128875
2214	6	0.08846631703035968
2214	7	0.1063138870364213
2214	8	0.04749143909565845
2214	9	0.35588816555909375
2214	10	0.4966228159602508
2214	11	-0.2115675837124371
2214	12	-0.3113091587631587
2214	13	-0.29111772575055994
2214	14	0.5801812136339279
2214	15	0.1747782727989673
2214	16	0.308259835713244
2214	17	0.22805981001162934
2214	18	0.11048463477899872
2214	19	-1.00826952572438
2214	20	-0.21742651624857245
2214	21	0.17186769109609656
2214	22	0.4468599079652691
2214	23	1.008580812354353
2214	24	0.9260364939600976
2214	25	0.7146090398154428
2215	1	-0.20979198098735452
2215	2	0.17067919109377172
2215	3	0.18335757879930625
2215	4	0.3021246023298758
2215	5	0.4540418713601042
2215	6	0.18116990179635195
2215	7	0.13587768383162313
2215	8	0.14275205253115075
2215	9	0.3133955263161923
2215	10	0.5368775880103343
2215	11	0.24350164833986554
2215	12	0.18449350262570618
2215	13	0.16122360139726055
2215	14	0.9955281756209331
2215	15	0.4727054047857422
2215	16	0.41318888470716786
2215	17	0.4246043392374119
2215	18	0.3114099482757382
2215	19	-0.3932305825887994
2215	20	-1.2870324240141358
2215	21	-1.8725626778572888
2215	22	-0.21404045012745598
2215	23	0.2934489913606271
2215	24	0.6947575481883348
2215	25	-0.24239469466259322
2216	1	0.12912724155529176
2216	2	-0.042918715643214256
2216	3	-0.057327162116031616
2216	4	0.38288760530445387
2216	5	0.39048579828548213
2216	6	0.17898460136208172
2216	7	0.17248743273889033
2216	8	0.03408470258693086
2216	9	0.201865032786543
2216	10	-1.8346039892111747
2216	11	-0.5340609197931983
2216	12	-0.19690041719013607
2216	13	0.05285811267285112
2216	14	-0.47939751038222056
2216	15	-0.07218888832284875
2216	16	0.10739119962640586
2216	17	0.5445503067513613
2216	18	0.3460049920372157
2216	19	-1.0823091562575584
2216	20	-0.8732228782749303
2216	21	-0.7289276637037574
2216	22	-0.4665265192993928
2216	23	-4.2702339777167495
2216	24	-1.7423672472233704
2216	25	-0.815411663095233
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	19.0
5	22.0
6	35.0
7	134.0
8	168.0
9	468.0
10	1045.0
11	2047.0
12	3954.0
13	6453.0
14	9819.0
15	14156.0
16	18405.0
17	21033.0
18	21014.0
19	19675.0
20	18716.0
21	17998.0
22	16827.0
23	16313.0
24	16033.0
25	16451.0
26	17227.0
27	18942.0
28	21737.0
29	26079.0
30	31340.0
31	38369.0
32	50862.0
33	68266.0
34	92369.0
35	126203.0
36	179294.0
37	263526.0
38	380950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.90750556953959	18.023740345751282	12.029293269572232	20.0394608151369
2	12.325570037345871	25.510192785441948	36.83234080793413	25.331896369278056
3	21.308292770608837	32.12333325952372	30.035389538920025	16.532984430947415
4	12.57651290054072	21.89646480764797	30.2749449315536	35.25207736025771
5	9.14590297626647	47.52073329286118	31.037407211512157	12.295956519360193
6	28.201369797004887	32.049772677251184	24.341506359957858	15.407351165786071
7	21.782859717875482	40.756260931966864	18.1355713666147	19.325307983542952
8	21.96312961295016	37.61392965228622	24.080633701229644	16.342307033533988
9	27.906373944134373	24.239548802646745	16.092010915224655	31.762066337994234
10	14.999742770382515	29.562325433887754	26.968995039701277	28.46893675602845
11	26.95827941920565	31.114252800658498	19.070200264257913	22.85726751587794
12	26.27152692628019	29.79351992878785	21.955885714136876	21.979067430795087
13	28.527201448312688	22.832870967321796	30.72161658786908	17.918310996496437
14	29.78817077896492	27.187604815573437	21.620973860827714	21.40325054463393
15	32.48130045295724	24.156460746418837	23.129735943183334	20.23250285744058
16	24.044776994158674	26.081687407610005	29.344625262924833	20.52891033530649
17	19.571297678904358	34.99427915012331	25.62582581135457	19.808597359617767
18	20.288944314563132	24.72437304228342	36.84994041378232	18.136742229371123
19	22.7211694118168	34.73436488638894	21.586947263684184	20.957518438110075
20	20.847308744423675	31.243386708496857	28.60256307574298	19.30674147133649
21	22.49396888236309	33.20230370787577	23.16190091712056	21.141826492640572
22	20.329725428501103	30.845437461174107	20.17155672129098	28.65328038903381
23	24.926336380158325	25.564613331926804	22.67771572126533	26.831334566649538
24	18.932375165070443	31.36260278646722	19.746657567714696	29.95836448074764
25	24.15228774442647	32.43251346211251	21.58220068226286	21.832998111198158
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11784.0
1	11784.0
2	10367.5
3	8951.0
4	8951.0
5	8951.0
6	12669.5
7	16388.0
8	16388.0
9	16388.0
10	14979.0
11	13570.0
12	13570.0
13	13570.0
14	13584.0
15	13598.0
16	13598.0
17	13598.0
18	16221.5
19	18845.0
20	18845.0
21	18845.0
22	23174.5
23	27504.0
24	27504.0
25	27504.0
26	33437.0
27	39370.0
28	39370.0
29	39370.0
30	42851.5
31	46333.0
32	46333.0
33	46333.0
34	52383.5
35	58434.0
36	58434.0
37	58434.0
38	91364.0
39	124294.0
40	124294.0
41	124294.0
42	284819.0
43	445344.0
44	445344.0
45	445344.0
46	353322.0
47	261300.0
48	261300.0
49	261300.0
50	209874.0
51	158448.0
52	158448.0
53	158448.0
54	128655.5
55	98863.0
56	98863.0
57	98863.0
58	85102.0
59	71341.0
60	71341.0
61	71341.0
62	63760.5
63	56180.0
64	56180.0
65	56180.0
66	44390.5
67	32601.0
68	32601.0
69	32601.0
70	25715.5
71	18830.0
72	18830.0
73	18830.0
74	14054.5
75	9279.0
76	9279.0
77	9279.0
78	6282.5
79	3286.0
80	3286.0
81	3286.0
82	2121.5
83	957.0
84	957.0
85	957.0
86	631.5
87	306.0
88	306.0
89	306.0
90	206.5
91	107.0
92	107.0
93	107.0
94	71.0
95	35.0
96	35.0
97	35.0
98	22.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08274965851930847
2	0.02037816138201696
3	0.030990430727923547
4	0.038803144356811835
5	0.048569036392922205
6	0.01666712240829502
7	0.021094326797998383
8	0.03066490099338654
9	0.03177170209081238
10	0.023763670621201882
11	0.021940704107794612
12	0.017253075930461638
13	0.024610047930998115
14	0.034050410232571465
15	0.03209723182534939
16	0.022656869523776043
17	0.022591763576868637
18	0.02395898846192409
19	0.018229665134072676
20	0.014648838054165544
21	0.011263328814980619
22	0.01666712240829502
23	0.016992652142832028
24	0.016276486726850604
25	0.1075550242910288
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1535958.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.626497438114153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.72968126916588	17.24273747709274
2	7.11421880302034	3.0771126943540583
3	3.0359812139216102	1.9697291983511518
4	1.826150019473674	1.579729148710381
5	1.2634277040720523	1.3661758002678344
6	0.928898114848075	1.2053287620618576
7	0.7249096177656358	1.0974079194030983
8	0.5891407379689819	1.0192840528299896
9	0.49065583824924586	0.9550050506003761
>10	3.693127873526921	15.509591452239233
>50	0.3300061039798323	4.9005243137666525
>100	0.20978516728586172	9.003118037917325
>500	0.029754629743947603	4.335552005348153
>1k	0.02644855977239787	11.526957635160066
>5k	0.0036066217871451645	5.343033881197398
>10k+	0.004207725418336025	19.86871257069972
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	77279	5.031322471057152	No Hit
GCTTATGTACTCTGCGTTGATACCA	46007	2.995329299368863	No Hit
CTTATGTACTCTGCGTTGATACCAC	29961	1.9506392752926835	No Hit
GAGTACATAAGCAGTGGTATCAACG	20419	1.32939832990225	No Hit
TATGTACTCTGCGTTGATACCACTG	18969	1.2349947068865164	No Hit
CCCATGTACTCTGCGTTGATACCAC	16828	1.095602874557768	No Hit
GCGTTGATACCACTGCTTATGTACT	15449	1.00582177377246	No Hit
CATAAGCAGTGGTATCAACGCAGAG	13670	0.8899982942241911	No Hit
ACTCTGCGTTGATACCACTGCTTAT	12516	0.8148660314930487	No Hit
CTGCTTATGTACTCTGCGTTGATAC	11493	0.748262647806776	No Hit
CATGTACTCTGCGTTGATACCACTG	11232	0.7312699956639439	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	11082	0.7215041036278336	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	10389	0.6763856824210037	No Hit
GTATCAACGCAGAGTACATAAGCAG	10384	0.6760601526864667	No Hit
TATCAACGCAGAGTACATAAGCAGT	9208	0.5994955591233615	No Hit
GGTATCAACGCAGAGTACATAAGCA	8512	0.5541818200758094	No Hit
GTACATGATAAGCAGTGGTATCAAC	8376	0.5453274112964026	No Hit
ATCATGTACTCTGCGTTGATACCAC	8310	0.5410304188005141	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	7066	0.46003862084770547	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6772	0.4408974724569291	No Hit
GTACATGGGAAGCAGTGGTATCAAC	6724	0.43777238700537385	No Hit
GTGGTATCAACGCAGAGTACATAAG	6013	0.3914820587542107	No Hit
ACATAAGCAGTGGTATCAACGCAGA	5528	0.3599056745041206	No Hit
GTGGTATCAACGCAGAGTACATGGG	5262	0.34258749262675153	No Hit
GTACTCTGCGTTGATACCACTGCTT	5221	0.33991814880354804	No Hit
GCAGTGGTATCAACGCAGAGTACAT	5210	0.3392019833875666	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4598	0.29935714388023626	No Hit
CCACTGCTTATGTACTCTGCGTTGA	4432	0.2885495566936075	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4419	0.2877031793838113	No Hit
GATACCACTGCTTATGTACTCTGCG	4343	0.2827551274188487	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4093	0.2664786406919981	No Hit
GTACATGGGATAAGCAGTGGTATCA	3914	0.25482467619557303	No Hit
ATACCACTGCTTATGTACTCTGCGT	3891	0.2533272394167028	No Hit
GTTGATACCACTGCTTATGTACTCT	3824	0.24896514097390685	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3614	0.23529289212335233	No Hit
GCTTATCCCATGTACTCTGCGTTGA	3584	0.23333971371613027	No Hit
GAGTACATGATAAGCAGTGGTATCA	3276	0.2132870820686503	No Hit
CAGTGGTATCAACGCAGAGTACATA	3207	0.20879477173203956	No Hit
TGATACCACTGCTTATGTACTCTGC	2901	0.18887235197837443	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2888	0.18802597466857818	No Hit
GATAAGCAGTGGTATCAACGCAGAG	2869	0.18678896167733755	No Hit
TACCACTGCTTATGTACTCTGCGTT	2808	0.18281749891598598	No Hit
GCTTATCATGTACTCTGCGTTGATA	2745	0.17871582426081964	No Hit
GCAGAGTACATAAGCAGTGGTATCA	2696	0.1755256328623569	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	2695	0.17546052691544953	No Hit
CCCCATGTACTCTGCGTTGATACCA	2675	0.17415840797730145	No Hit
GTATCAACGCAGAGTACATGGGAAG	2531	0.16478315162263552	No Hit
GGATAAGCAGTGGTATCAACGCAGA	2486	0.1618533840118024	No Hit
GGTATCAACGCAGAGTACATGGGAA	2469	0.16074658291437655	No Hit
GGATACCACGTGTCCCGCCCTACTC	2422	0.15768660340972865	No Hit
ATACAGGGTGACAGCCCCGTACACA	2397	0.1560589547370436	No Hit
GCGTTGATACCACTGCTTATCATGT	2383	0.15514747148033997	No Hit
GCGTTGATACCACTGCTTCCCATGT	2299	0.14967857194011813	No Hit
ATCAACGCAGAGTACATAAGCAGTG	2189	0.14251691778030387	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2172	0.14141011668287803	No Hit
TATCAACGCAGAGTACATGGGAAGC	2057	0.13392293278852677	No Hit
GGGATAAGCAGTGGTATCAACGCAG	2045	0.13314166142563794	No Hit
ACGCAGAGTACATGATAAGCAGTGG	2045	0.13314166142563794	No Hit
GAGTACATGGGATAAGCAGTGGTAT	2020	0.13151401275295288	No Hit
CAGTGGTATCAACGCAGAGTACATG	2013	0.13105827112460106	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	2004	0.13047231760243444	No Hit
GTACACAAGCAGTGGTATCAACGCA	1988	0.129430622451916	No Hit
TCCATGTACTCTGCGTTGATACCAC	1980	0.12890977487665678	No Hit
TATCAACGCAGAGTACATGATAAGC	1916	0.12474299427458303	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1894	0.12331066344262018	No Hit
GTATCAACGCAGAGTACATGGGATA	1892	0.12318045154880537	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1855	0.12077153151323149	No Hit
ATGTACTCTGCGTTGATACCACTGC	1813	0.11803708174312058	No Hit
GTATCAACGCAGAGTACATGATAAG	1779	0.1158234795482689	No Hit
GGTATCAACGCAGAGTACATGGGAT	1779	0.1158234795482689	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1743	0.1134796654596024	No Hit
TACATAAGCAGTGGTATCAACGCAG	1609	0.1047554685740105	No Hit
GCGTTGATACCACTGCTTATCCCAT	1597	0.10397419721112167	No Hit
ATGATAAGCAGTGGTATCAACGCAG	1588	0.10338824368895505	No Hit
GTACATGGTAAGCAGTGGTATCAAC	1581	0.10293250206060323	No Hit
GTACATAAGCAGTTGTATCAACGCA	1575	0.10254186637915881	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	1572	0.10234654853843661	No Hit
CCCATATTCAGACAGGATACCACGT	1537	0.1000678403966775	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.510594690740242E-5	0.0	0.0	6.510594690740242E-5	0.0
2	6.510594690740242E-5	0.0	0.0	6.510594690740242E-5	0.0
3	6.510594690740242E-5	0.0	0.0	6.510594690740242E-5	0.0
4	6.510594690740242E-5	0.0	0.0	6.510594690740242E-5	0.0
5	6.510594690740242E-5	0.0	0.0	6.510594690740242E-5	0.0
6	6.510594690740242E-5	0.0	0.0	6.510594690740242E-5	0.0
7	1.3021189381480483E-4	0.0	0.0	6.510594690740242E-5	0.0
8	1.3021189381480483E-4	0.0	0.0	1.3021189381480483E-4	0.0
9	1.3021189381480483E-4	0.0	0.0	1.3021189381480483E-4	0.0
10	1.3021189381480483E-4	0.0	0.0	1.3021189381480483E-4	0.0
11	1.3021189381480483E-4	0.0	0.0	1.3021189381480483E-4	0.0
12	1.3021189381480483E-4	0.0	0.0	1.3021189381480483E-4	0.0
13	1.3021189381480483E-4	0.0	0.0	1.9531784072220724E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTAGC	25	0.006024645	19.00404	2
AACCGTC	25	0.0060416684	18.994757	16
CAGTTGT	245	0.0	16.671041	10
ACTTCTC	40	2.76622E-4	16.620955	18
AGTTGTA	250	0.0	16.337088	11
AGACATT	35	0.0021727695	16.282812	6
TGGTTGG	50	8.727093E-5	15.19729	6
TATACTG	90	2.2791937E-9	14.781401	5
TGTATCA	305	0.0	14.324849	14
CGCCAGA	40	0.0052864524	14.246997	14
GTACAAA	475	0.0	14.211815	1
TTGTATC	290	0.0	14.083696	13
GTTGTAT	295	0.0	13.844989	12
ACTGCCT	55	1.9630931E-4	13.81572	8
AATCGCC	85	2.7069473E-7	13.408501	18
ATGGTTG	65	5.4398777E-5	13.157071	5
AGACTAT	80	2.0033622E-6	13.060172	6
TACTCTT	240	0.0	13.060172	8
CGGCGTT	110	2.752131E-9	12.952659	9
TCGTTGA	220	0.0	12.951816	14
>>END_MODULE