FastQCFastQC Report
Wed 25 May 2016
SRR1295353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295353_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535958
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA822495.354899027186941No Hit
GCTTATGTACTCTGCGTTGATACCA518173.3735948509008713No Hit
CTTATGTACTCTGCGTTGATACCAC358832.3361966928783207No Hit
GAGTACATAAGCAGTGGTATCAACG216451.4092182208107253No Hit
GTATCAACGCAGAGTACTTTTTTTT212631.3843477490920975No Hit
CCCATGTACTCTGCGTTGATACCAC202571.3188511665032507No Hit
GCGTTGATACCACTGCTTATGTACT176731.1506173996945228No Hit
TATGTACTCTGCGTTGATACCACTG173941.1324528405073575No Hit
CATAAGCAGTGGTATCAACGCAGAG170761.1117491493908036No Hit
ACTCTGCGTTGATACCACTGCTTAT144630.9416273101217612No Hit
GGTATCAACGCAGAGTACTTTTTTT141450.9209236190052073No Hit
ACGCAGAGTACATAAGCAGTGGTAT134920.8784094356746733No Hit
CTGCTTATGTACTCTGCGTTGATAC133670.870271192311248No Hit
CATGTACTCTGCGTTGATACCACTG131930.8589427575493601No Hit
TATCAACGCAGAGTACTTTTTTTTT127500.8301008230693808No Hit
GTACTTTTTTTTTTTTTTTTTTTTT126000.8203349310332705No Hit
GTATCAACGCAGAGTACATAAGCAG117100.7623906382856823No Hit
TATCAACGCAGAGTACATAAGCAGT100310.6530777534281537No Hit
ATCATGTACTCTGCGTTGATACCAC94510.6153163042218602No Hit
GGTATCAACGCAGAGTACATAAGCA92340.6011883137429539No Hit
GTACATGATAAGCAGTGGTATCAAC86860.5655102548376973No Hit
AAGCAGTGGTATCAACGCAGAGTAC78600.511732742692183No Hit
ACGCAGAGTACTTTTTTTTTTTTTT78280.5096493523911461No Hit
ACATAAGCAGTGGTATCAACGCAGA71540.46576794417555695No Hit
GTACATGGGAAGCAGTGGTATCAAC68960.44897060987344706No Hit
GTGGTATCAACGCAGAGTACATAAG64770.4216912181192455No Hit
GCAGTGGTATCAACGCAGAGTACAT59460.38711996031141477No Hit
GTGGTATCAACGCAGAGTACATGGG57740.3759217374433415No Hit
GTACTCTGCGTTGATACCACTGCTT54270.3533299738664729No Hit
CCACTGCTTATGTACTCTGCGTTGA52450.3414806915293257No Hit
GATACCACTGCTTATGTACTCTGCG49840.3244880393864936No Hit
ATACCACTGCTTATGTACTCTGCGT44580.2902423113132No Hit
GTACATGGGATAAGCAGTGGTATCA41130.26778075963014614No Hit
GAGTACTTTTTTTTTTTTTTTTTTT38940.253522557257425No Hit
CAGTGGTATCAACGCAGAGTACATA38920.2533923453636102No Hit
GTACTGGTTCACTATCGGTCAGTCA38570.2511136372218511No Hit
GCTTATCCCATGTACTCTGCGTTGA38520.2507881074873141No Hit
GTTGATACCACTGCTTATGTACTCT37470.24395198306203686No Hit
GAGTACATGATAAGCAGTGGTATCA34960.22761039038827882No Hit
TACCACTGCTTATGTACTCTGCGTT34500.22461551683053832No Hit
GATAAGCAGTGGTATCAACGCAGAG33920.22083937190990902No Hit
CCCCATGTACTCTGCGTTGATACCA32500.21159432744905787No Hit
TGATACCACTGCTTATGTACTCTGC32250.2099666787763728No Hit
GCAGAGTACATAAGCAGTGGTATCA30800.20052631647479946No Hit
GCTTATCATGTACTCTGCGTTGATA30770.20033099863407725No Hit
GAGTACATGGGAAGCAGTGGTATCA30290.1972059131825219No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA29940.19492720504076283No Hit
GGATAAGCAGTGGTATCAACGCAGA29910.19473188720004064No Hit
GCAGAGTACTTTTTTTTTTTTTTTT29820.194145933677874No Hit
CTTATACACATCTCCGAGCCCACGA28150.1832732405443378No Hit
GCGTTGATACCACTGCTTATCATGT27630.17988773130515287No Hit
ATACAGGGTGACAGCCCCGTACACA27490.17897624804844922No Hit
ATCAACGCAGAGTACATAAGCAGTG27030.17598137449070872No Hit
GGATACCACGTGTCCCGCCCTACTC26980.17565584475617171No Hit
GGGATAAGCAGTGGTATCAACGCAG26380.17174948794172756No Hit
GTATCAACGCAGAGTACATGGGAAG26120.1700567333221351No Hit
GCGTTGATACCACTGCTTCCCATGT25980.1691452500654315No Hit
GGTATCAACGCAGAGTACATGGGAA25390.16530399919789474No Hit
ACGCAGAGTACATGATAAGCAGTGG25240.1643274099942837No Hit
CAGTGGTATCAACGCAGAGTACATG25090.16335082079067267No Hit
ACGCAGAGTACATGGGAAGCAGTGG24170.15736107367519164No Hit
TATCAACGCAGAGTACATGGGAAGC23030.14993899572774777No Hit
TCCATGTACTCTGCGTTGATACCAC22730.14798581732052568No Hit
AAAAAGTACTCTGCGTTGATACCAC21920.1427122356210261No Hit
GTACACAAGCAGTGGTATCAACGCA21510.1400428917978226No Hit
ACTCTGCGTTGATACCACTGCTTCC21020.1368527003993599No Hit
GAGTACATGGGATAAGCAGTGGTAT20990.13665738255863766No Hit
TACATAAGCAGTGGTATCAACGCAG20780.1352901576735822No Hit
ATCAACGCAGAGTACTTTTTTTTTT20330.13236039006274913No Hit
TATCAACGCAGAGTACATGATAAGC20230.1317093305936751No Hit
GTATCAACGCAGAGTACATGGGATA20130.13105827112460106No Hit
CCATGTACTCTGCGTTGATACCACT19700.12825871540758277No Hit
GTGGTATCAACGCAGAGTACTTTTT19050.12402682885860161No Hit
GTATCAACGCAGAGTACATGATAAG18600.12109706124776849No Hit
ACCCTGTATCGCGCGCCTTTCCAGA18480.12031578988487966No Hit
CCCATATTCAGACAGGATACCACGT18420.11992515420343525No Hit
ATGTACTCTGCGTTGATACCACTGC18350.11946941257508342No Hit
ATGATAAGCAGTGGTATCAACGCAG18280.11901367094673161No Hit
GGTATCAACGCAGAGTACATGGGAT18130.11803708174312058No Hit
GCGTTGATACCACTGCTTATCCCAT17110.11139627515856552No Hit
CATGTAAGCAGTGGTATCAACGCAG16680.10859671944154724No Hit
GGTATCAACGCAGAGTACATGATAA16610.1081409778131954No Hit
CTGCTTATCATGTACTCTGCGTTGA16250.10579716372452894No Hit
ACGCAGAGTACATGGGATAAGCAGT15830.10306271395441802No Hit
GTACATGGTAAGCAGTGGTATCAAC15730.10241165448534399No Hit
CTATCGGTCAGTCAGGAGTATTTAG15560.10130485338791817No Hit
CTGCGTTGATACCACTGCTTATGTA15550.10123974744101075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGCT250.006044238318.99335910
CTTATAC2300.017.790571
GTCTACA453.4884913E-516.917131
TGATCAG350.002160165516.2969995
ATAACGT350.002175257516.28002413
CAACCAT551.1320357E-515.540026
TTATACA2650.015.4262792
GGCTCCG508.7398555E-515.19468818
TATACAC2950.015.1465743
TCTAGTA456.725179E-414.78852
CGCATAA456.780211E-414.77261310
CTGATCA456.780211E-414.7726139
CCGCATA456.780211E-414.7726139
ATGACTT400.005291323614.24501911
AAAAGTA3600.013.7321792
AAGGCCG707.2106322E-613.58083255
CCTCCTC500.001503488913.2953518
GTAGAAG801.9831405E-613.0719773
TAGGACC801.9831405E-613.0719774
AAAAAGT3800.013.0217391
Produced by FastQC (version 0.11.5)