FastQCFastQC Report
Wed 25 May 2016
SRR1295352_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295352_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4340842
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG66410.15298875195181028No Hit
GTATCAACGCAGAGTACTTTTTTTT57900.13338426047296814No Hit
GGTATCAACGCAGAGTACTTTTTTT44740.10306756154681511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC3000.010.4519213
GGTATCA38000.09.9317471
GTGGTAT19450.09.67752551
TAATACG1106.827678E-59.5018564
TGGTATC19250.09.2794132
ACACCGT1554.2738066E-79.1923786
GTCCTAC2701.8189894E-128.8022771
ACTGATC4150.08.6976878
CTAGACT4850.08.620244
GTCTTAC4550.08.5662581
GTACTAC3250.08.4826861
CAGGACT5550.08.3890274
GTCTTAG4600.08.2664851
TCTAGAC4150.08.2424793
CAGTACT4450.08.1139444
GGTCTAC2355.460606E-98.0906031
GTTCTAG5550.08.0505141
TACACCG1304.179656E-48.0400315
CCCGTGC1553.907449E-57.96672738
GTATCAA65200.07.9463491
Produced by FastQC (version 0.11.5)