##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295352_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4340842
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.371124772567164	31.0	30.0	33.0	27.0	34.0
2	30.213834090252536	31.0	30.0	34.0	26.0	34.0
3	30.72173002380644	31.0	31.0	34.0	27.0	34.0
4	34.003734759293245	35.0	35.0	37.0	28.0	37.0
5	34.46296294589852	37.0	35.0	37.0	31.0	37.0
6	34.666130211604106	37.0	35.0	37.0	31.0	37.0
7	34.531031767569516	37.0	35.0	37.0	30.0	37.0
8	34.52247582381483	37.0	35.0	37.0	30.0	37.0
9	35.604190615553385	39.0	35.0	39.0	29.0	39.0
10	35.52336804702866	39.0	35.0	39.0	28.0	39.0
11	35.93502573924598	39.0	35.0	39.0	30.0	39.0
12	35.93748816473855	39.0	35.0	39.0	30.0	39.0
13	35.8647080451212	39.0	35.0	39.0	30.0	39.0
14	36.16871058656362	39.0	36.0	41.0	27.0	41.0
15	36.777920735193774	40.0	36.0	41.0	30.0	41.0
16	36.894516778081304	40.0	36.0	41.0	30.0	41.0
17	36.69675376344036	40.0	36.0	41.0	29.0	41.0
18	36.65070186843935	39.0	36.0	41.0	29.0	41.0
19	36.684742268896215	40.0	36.0	41.0	28.0	41.0
20	36.675125471049164	40.0	36.0	41.0	28.0	41.0
21	36.67406876361775	40.0	36.0	41.0	28.0	41.0
22	36.44931697583095	39.0	36.0	41.0	27.0	41.0
23	36.13553660787469	39.0	35.0	41.0	27.0	41.0
24	36.4464260620405	39.0	36.0	41.0	27.0	41.0
25	35.3321758773989	39.0	34.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3695778994245913
1101	2	0.4804956946412382
1101	3	0.32677169641171133
1101	4	0.3148591803523857
1101	5	0.3340594637661951
1101	6	0.41832310633944303
1101	7	0.29193651237702056
1101	8	0.40666180502790894
1101	9	0.4353798573509877
1101	10	0.6682437199105422
1101	11	0.5323458728934654
1101	12	0.5699646693363789
1101	13	0.5150233658682097
1101	14	1.0688681308650274
1101	15	0.8680351496862428
1101	16	0.7799937504102559
1101	17	0.9711987762533383
1101	18	0.9925690041058175
1101	19	0.8427962555219182
1101	20	0.9584549333846724
1101	21	0.9925814104155819
1101	22	0.7076736059673152
1101	23	-1.4510573455787465
1101	24	0.29707195554266264
1101	25	0.6886531758785779
1102	1	0.27404802734750433
1102	2	0.3303023942619312
1102	3	0.30635776366260004
1102	4	0.44064210381640123
1102	5	0.36386646073807327
1102	6	0.32810114639054433
1102	7	0.22003760337952372
1102	8	0.3042671275920412
1102	9	0.4513336604726632
1102	10	0.8997150379014371
1102	11	0.6347356036878367
1102	12	0.5402545049442864
1102	13	0.5653674918336904
1102	14	1.1660890358251805
1102	15	0.8415692636051304
1102	16	0.6512340541638935
1102	17	0.9268055429051714
1102	18	0.8976968695377181
1102	19	0.9126568442418943
1102	20	0.9616292954779482
1102	21	0.9214248016251787
1102	22	0.8453261753137014
1102	23	0.8999683833682894
1102	24	0.891873068498306
1102	25	0.9117280259151954
1103	1	-0.24430881679527872
1103	2	-1.526434954436862
1103	3	-0.2918466457362001
1103	4	0.0662734893085215
1103	5	0.03066368547287368
1103	6	0.16162833169899926
1103	7	0.011838740828572725
1103	8	0.29030593020942064
1103	9	0.8924799361440989
1103	10	0.9580915837112656
1103	11	0.7194129543659997
1103	12	0.48332836778293853
1103	13	0.3181051834703794
1103	14	-0.8284416801324781
1103	15	0.06878984402176513
1103	16	0.16550372303318284
1103	17	0.7183869354171577
1103	18	0.707553284432727
1103	19	0.6968066114353988
1103	20	0.7537042078446561
1103	21	0.8683734023695138
1103	22	0.44991112839565517
1103	23	1.202697156318088
1103	24	0.9539237105286844
1103	25	0.9442318638275609
1104	1	-0.012417830831502386
1104	2	-0.004429683344799429
1104	3	-0.30395213789768505
1104	4	-0.25697615799125373
1104	5	-0.11839219132597378
1104	6	0.0230740583493656
1104	7	-0.03310002924018107
1104	8	0.04125928294919845
1104	9	-2.209955783049388
1104	10	-0.48753427423294937
1104	11	-0.31830509348402813
1104	12	0.12542795596971246
1104	13	0.2511864869156355
1104	14	-1.675934248888879
1104	15	-0.45337496623796625
1104	16	-0.09658470180686862
1104	17	0.28004552745836264
1104	18	0.35728379655492404
1104	19	0.42030809328910124
1104	20	0.5669551883000068
1104	21	0.7211435092124105
1104	22	0.7824768961833897
1104	23	1.1663931323857497
1104	24	0.8156385588053254
1104	25	0.687888753531638
1105	1	-0.16137693945196574
1105	2	-0.021291231236077124
1105	3	-1.4404881858725922
1105	4	-0.5449156651079008
1105	5	-0.3457948938435962
1105	6	-0.07694533435314099
1105	7	-0.07644314031237087
1105	8	-0.11107151782628222
1105	9	0.28416898583787287
1105	10	0.5101068041697019
1105	11	0.04310213755238124
1105	12	0.0458985952497315
1105	13	-0.16521019740933696
1105	14	0.6149602177640929
1105	15	0.28247231805399764
1105	16	-0.10723208129427775
1105	17	0.38547088155058873
1105	18	0.2813290076519266
1105	19	0.08480159855796643
1105	20	-0.3622418864609642
1105	21	0.12809810839808478
1105	22	0.2717869986868777
1105	23	0.38834819744531757
1105	24	0.2682010897466469
1105	25	0.45588473242250416
1106	1	-1.579398562751475
1106	2	-0.972252219306668
1106	3	-1.0750266344134296
1106	4	-1.3177819080228232
1106	5	-0.8031821662929985
1106	6	-0.6461525417399443
1106	7	-0.5986374225116649
1106	8	-0.5837589192807471
1106	9	-0.11776153368518294
1106	10	-1.6336789059799344
1106	11	-1.0458617590651045
1106	12	-0.7129230398206587
1106	13	-0.7557709944878752
1106	14	-0.06748702447970345
1106	15	-0.7859410667674496
1106	16	-1.1716471634009196
1106	17	-0.49212998493818816
1106	18	-0.5223943880196202
1106	19	-0.4199577770916889
1106	20	-0.20288182847987457
1106	21	-0.13237038218568387
1106	22	-0.22184580865891235
1106	23	-0.4585097840193413
1106	24	-0.6109890830859683
1106	25	-1.3526367214332708
1107	1	-0.06395850673765935
1107	2	0.09626852512380779
1107	3	-0.05106089273779446
1107	4	0.14111095598808987
1107	5	-0.024904941884017262
1107	6	0.04307259424012955
1107	7	0.004860318364841021
1107	8	-0.08755008287695887
1107	9	0.41061643400858117
1107	10	-0.0642742594852308
1107	11	0.028668562821920318
1107	12	-0.10451229771126691
1107	13	0.11685719522593985
1107	14	-0.9765329083317411
1107	15	-0.5153146145110838
1107	16	-0.19470077290225163
1107	17	0.014885541017626736
1107	18	0.39443649157563243
1107	19	0.2482366140719705
1107	20	0.3119158894497005
1107	21	0.44520117743251575
1107	22	-0.1850919920414924
1107	23	0.6094041367399754
1107	24	0.4217537764004362
1107	25	0.2201427288937765
1108	1	-0.11995452211346702
1108	2	0.12708560164756832
1108	3	-0.13308278759558334
1108	4	-0.2822199615404699
1108	5	-0.3182723389145323
1108	6	-0.2158297984353652
1108	7	-0.16028688716271944
1108	8	-0.2610942339792146
1108	9	0.22139044023546006
1108	10	0.20207523943319927
1108	11	-0.02498878266710136
1108	12	-0.08237424920650227
1108	13	-0.22773020558058477
1108	14	0.08363606202685503
1108	15	-0.15910136009373588
1108	16	-0.16458457497372336
1108	17	0.12860573768676886
1108	18	0.1986311087367767
1108	19	0.19819883832816743
1108	20	0.2456671288300072
1108	21	0.2826375545680406
1108	22	0.20117220092338783
1108	23	0.035218600875694506
1108	24	0.06443198471866651
1108	25	-0.6566992439720067
1109	1	-0.8267934552071239
1109	2	-0.7578939114244143
1109	3	-0.7754948259788357
1109	4	-0.6820054876359691
1109	5	-0.9886543650496833
1109	6	-1.0945893039293821
1109	7	-0.7254127165528672
1109	8	-1.2089490984130293
1109	9	-0.860407254654703
1109	10	-1.0351141302743443
1109	11	-1.1620759380241026
1109	12	-1.095949768869552
1109	13	-1.1750755843464376
1109	14	-0.6796824237909007
1109	15	-1.317680404389563
1109	16	-1.5364189701026802
1109	17	-1.3117595905005999
1109	18	-2.0555350512639237
1109	19	-2.1172640781292245
1109	20	-1.7465935706333653
1109	21	-1.5721415390726392
1109	22	-1.662157828066718
1109	23	-1.2371594688591614
1109	24	-2.1290637901277947
1109	25	-1.2676737608770239
1110	1	0.040719179940978734
1110	2	0.08938457363732155
1110	3	0.019258868602445745
1110	4	0.19603259907206905
1110	5	0.08715769645203153
1110	6	0.09957656304436568
1110	7	0.0735096172709433
1110	8	-0.001521657217111283
1110	9	0.3105718893539944
1110	10	0.25718088177436726
1110	11	0.18865849995171402
1110	12	0.12371318722198055
1110	13	0.11509597644089098
1110	14	-2.4789706970939562
1110	15	-0.6911873243989461
1110	16	-0.17248341468928885
1110	17	-0.20289131591803766
1110	18	-2.607064170748714
1110	19	-0.5151494768028044
1110	20	-1.781468498660999
1110	21	-0.29790050096460874
1110	22	-1.655198047289204
1110	23	-0.554963192229387
1110	24	0.10679113491432446
1110	25	0.09339366922981185
1111	1	-0.14613706503718404
1111	2	-1.3612175578960795
1111	3	-0.40082144168418665
1111	4	0.011838301190451261
1111	5	-0.09337681540451825
1111	6	-0.020262014355395763
1111	7	-0.0760511017951444
1111	8	-0.029742052274080777
1111	9	-1.698531899294288
1111	10	-0.41608555403242065
1111	11	-0.1783328083254503
1111	12	-0.10760674867454867
1111	13	-0.019806036891338863
1111	14	0.8504695125285124
1111	15	0.2373485509034694
1111	16	0.27019273415321976
1111	17	-0.22885605900777506
1111	18	0.09523368544296318
1111	19	-0.1626230184707964
1111	20	0.28047889130358783
1111	21	-0.4827251724881023
1111	22	-0.16310050711572188
1111	23	-0.05508921805368061
1111	24	-0.45311974228312124
1111	25	-1.8437521612813512
1112	1	0.1819732224256576
1112	2	0.4347637873973369
1112	3	0.0702021315335628
1112	4	0.3393989061206568
1112	5	0.05343461887886747
1112	6	0.09007689972354882
1112	7	0.13758828258202982
1112	8	-0.0055549031430786044
1112	9	-0.2450233187942885
1112	10	0.5052171682354825
1112	11	0.10750929914858887
1112	12	0.11150822481884148
1112	13	0.11288490108690752
1112	14	0.48901206025340116
1112	15	0.1799713849148219
1112	16	0.20938904978254413
1112	17	-3.5122698803715693
1112	18	-0.8770759731486351
1112	19	-1.0769146246072765
1112	20	-0.07785223691012533
1112	21	-0.4976646227440966
1112	22	-1.5706242169251468
1112	23	-1.418804507910714
1112	24	-0.3632157439238952
1112	25	0.028502050310848404
1113	1	-0.2857655288850829
1113	2	-0.11218386983201967
1113	3	-0.17467783251478153
1113	4	-0.4930690645818956
1113	5	-0.19067159618456486
1113	6	-0.2106558517606416
1113	7	-0.33179714911376834
1113	8	-0.24477500453305368
1113	9	0.22094449816763984
1113	10	0.25313898915524646
1113	11	-0.22306821372226437
1113	12	-0.20001096910721827
1113	13	-0.5150607299198171
1113	14	-1.5607489716221536
1113	15	-1.7287915155006743
1113	16	-0.5399780907206235
1113	17	-0.317582525735304
1113	18	-0.7261737916085522
1113	19	-0.195754868384725
1113	20	0.15663281082831304
1113	21	-1.5847172966483996
1113	22	-2.0225767228973766
1113	23	-0.6737310671954688
1113	24	-2.37680294639636
1113	25	-0.7418953889221811
1114	1	0.058952110203073005
1114	2	0.20094431843515892
1114	3	-0.04820315931216612
1114	4	0.23703276070106227
1114	5	-0.06351901767348522
1114	6	0.07695633539064772
1114	7	0.0919948961596262
1114	8	0.05742978185304537
1114	9	0.2751780188116655
1114	10	0.09865758073821951
1114	11	-0.04121304075876964
1114	12	-0.013051579660384505
1114	13	-0.03159627909883511
1114	14	0.672112199268966
1114	15	0.2092454086185711
1114	16	0.18320778525578163
1114	17	-0.542567979266309
1114	18	-0.279494770406572
1114	19	-0.5654343479001085
1114	20	-1.764874243212816
1114	21	-0.8982335568110003
1114	22	-1.4836106181627997
1114	23	-2.950759801453394
1114	24	-1.9635856859149357
1114	25	-1.6152504423256602
1115	1	0.3398238653787793
1115	2	0.4213081100171472
1115	3	0.20201223010166558
1115	4	0.4194435096382847
1115	5	0.2948185838527664
1115	6	0.12422163029939526
1115	7	0.22579373376451883
1115	8	0.2418541419920075
1115	9	0.46769295842219094
1115	10	0.6573571898727693
1115	11	0.2879309786081663
1115	12	0.22345248163897224
1115	13	0.16582712913021425
1115	14	0.7280901417218786
1115	15	0.3634035731946028
1115	16	0.29371326400673325
1115	17	0.3359638334554873
1115	18	0.5210722476424294
1115	19	0.02844645709167537
1115	20	0.46016275194944
1115	21	-0.4874251953680755
1115	22	0.4997467186893445
1115	23	0.5983907938613058
1115	24	0.5419265340242703
1115	25	0.6467050809217412
1116	1	0.4120170064282931
1116	2	0.5103645834025379
1116	3	0.3290083513752968
1116	4	0.6641359965297795
1116	5	0.359606493098525
1116	6	0.20732446110396552
1116	7	0.1830692434282284
1116	8	0.21404943107597063
1116	9	0.40114544740926306
1116	10	0.37692462819422445
1116	11	0.28428874870453313
1116	12	0.1964421748308851
1116	13	-0.28530325096058107
1116	14	0.6727714914446423
1116	15	0.46774998266994317
1116	16	0.34239569299274564
1116	17	0.588115416064177
1116	18	0.527426190267505
1116	19	0.6210122729027532
1116	20	0.43723973644084424
1116	21	0.025623546794697916
1116	22	-0.01779539962283394
1116	23	-0.8672046345542412
1116	24	-0.3856487909155675
1116	25	-0.9278074198235089
1201	1	0.47170509411589023
1201	2	0.6900654696200732
1201	3	0.5994586314679751
1201	4	0.4252652248076174
1201	5	0.4583751723455478
1201	6	0.3969147660254819
1201	7	0.3827817950525514
1201	8	0.4341920202201166
1201	9	0.7699088720377532
1201	10	0.9785969835232038
1201	11	0.7473818714536549
1201	12	0.7292480873522393
1201	13	0.6698502699411222
1201	14	1.2426882241781527
1201	15	0.869688833199092
1201	16	0.6652718745136994
1201	17	0.7334912557276354
1201	18	0.7344085335200816
1201	19	1.0061309089758694
1201	20	0.9630612891345294
1201	21	0.9362822600891931
1201	22	1.1644552441447615
1201	23	0.7210543851060223
1201	24	1.0674561969773038
1201	25	1.2006821385427031
1202	1	0.3454864579506882
1202	2	0.7035386788578215
1202	3	0.5330436873074085
1202	4	0.39894243763082926
1202	5	0.36252152095465817
1202	6	0.2680324738891642
1202	7	0.2291484857833126
1202	8	0.34809903172794066
1202	9	0.8289568709606812
1202	10	0.11173141906363782
1202	11	0.44597500531443046
1202	12	0.4082090239017546
1202	13	0.3912658827972493
1202	14	1.0497927008154022
1202	15	0.7794067130002844
1202	16	0.6407063700801814
1202	17	-1.2920042163759007
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2210	19	0.7051287671207618
2210	20	0.8651646711248802
2210	21	0.8571503595049705
2210	22	1.107929465592008
2210	23	1.406706665006972
2210	24	1.0272401440332857
2210	25	0.971453540393135
2211	1	0.10118124456089106
2211	2	-0.5500330242501192
2211	3	7.895388879362031E-4
2211	4	0.3679349999980204
2211	5	0.2767144578187839
2211	6	0.23016049207093658
2211	7	0.27967700058182743
2211	8	0.26695046539080636
2211	9	0.7866939206397632
2211	10	0.8323423462347179
2211	11	0.4900354906571849
2211	12	0.3760717931484905
2211	13	0.20179403529563444
2211	14	-0.7326728881411597
2211	15	0.07694434301398445
2211	16	0.2191410997912442
2211	17	0.33369059726454253
2211	18	0.30255502046706084
2211	19	0.5690005703291305
2211	20	0.7224797795326126
2211	21	0.610381157226314
2211	22	-0.07303049809137008
2211	23	0.45342830654040256
2211	24	-0.17411381811099602
2211	25	0.25900792272909
2212	1	-0.05558841813751769
2212	2	-1.1233937709629664
2212	3	-0.3461068103937208
2212	4	0.08318987591489702
2212	5	0.042307673717672856
2212	6	0.02406544183539694
2212	7	0.07862970280394421
2212	8	0.047963375784817686
2212	9	-2.049712336916258
2212	10	-0.05398005677025708
2212	11	-0.031273475673877726
2212	12	-0.05895399492698061
2212	13	0.022314458769123746
2212	14	0.5314894521633207
2212	15	0.2651141676915927
2212	16	0.2550779824946332
2212	17	0.25306593195490734
2212	18	0.08134578857337971
2212	19	-0.555054802788149
2212	20	0.2765643691025659
2212	21	0.2736059601029126
2212	22	-1.8742660322365907
2212	23	-0.5116793960634922
2212	24	0.05541271616854715
2212	25	-0.6206468411354749
2213	1	0.12075043379462969
2213	2	0.4153922962551171
2213	3	0.11451634793992582
2213	4	0.2922107645400587
2213	5	0.14696986064355855
2213	6	0.10060228595393284
2213	7	0.08162368532578768
2213	8	0.08518654618863764
2213	9	0.5177817937098439
2213	10	0.500713436838879
2213	11	-0.06883275291947655
2213	12	-0.06952719556186793
2213	13	-0.007538487334514343
2213	14	-1.796859485588051
2213	15	-0.4821438371926803
2213	16	-0.20848014979761587
2213	17	-2.244823156355018
2213	18	-0.9573809786593515
2213	19	-0.5417466320767446
2213	20	0.12647178782834345
2213	21	-0.3159955053158541
2213	22	0.18462227080553362
2213	23	0.7003773692205968
2213	24	0.5050463994503858
2213	25	-1.0031660108381857
2214	1	0.07486756095216052
2214	2	0.08745729382298606
2214	3	-0.009361928375348327
2214	4	0.30611842740836437
2214	5	0.2422772696362756
2214	6	0.18339259114593887
2214	7	0.15729166288161878
2214	8	0.10563381258857163
2214	9	0.38572214778957914
2214	10	0.39026060904503623
2214	11	-0.2926901540016402
2214	12	-0.2848450072976618
2214	13	-0.08996012758738203
2214	14	0.6809862584175761
2214	15	0.3489273105918542
2214	16	0.2355082606826855
2214	17	0.2395570359717354
2214	18	0.18493475638581458
2214	19	-0.9788562396521812
2214	20	-0.043702957465932
2214	21	0.31769203309657
2214	22	0.44041187123308845
2214	23	0.861739905507342
2214	24	0.6256115095821073
2214	25	0.6331014071818757
2215	1	-0.140373759561367
2215	2	0.2462153247394987
2215	3	0.09774134928018441
2215	4	0.28329616198973895
2215	5	0.1844337940346037
2215	6	0.08409765301995975
2215	7	0.13785035354393216
2215	8	0.03784998088324443
2215	9	0.16345113324375404
2215	10	0.47846034875638566
2215	11	0.2178860460659564
2215	12	0.07977869918384783
2215	13	-0.037965379817059386
2215	14	0.8061345210916997
2215	15	0.18566666006348242
2215	16	0.19103581744323606
2215	17	0.37030166618055205
2215	18	0.21840908993586083
2215	19	-0.460244903736438
2215	20	-1.65572113387028
2215	21	-1.9029255935734568
2215	22	-0.33585454432428463
2215	23	0.29488975890720326
2215	24	0.3946006220630238
2215	25	-0.29900346662253696
2216	1	-0.020682794399139226
2216	2	-0.23710512255021854
2216	3	-0.22943898365128845
2216	4	0.14237046333312975
2216	5	0.08578365665018595
2216	6	-0.12281449341219997
2216	7	-0.053141695691984125
2216	8	-0.11763551679676709
2216	9	0.16069572729998782
2216	10	-1.6760190973548177
2216	11	-0.5994834718323858
2216	12	-0.3630109721743011
2216	13	-0.1730326538684963
2216	14	-0.5606207370027931
2216	15	-0.5778102338905811
2216	16	-0.18582627638938476
2216	17	0.08152866511989032
2216	18	-0.03477946632016682
2216	19	-1.4895887798626575
2216	20	-1.2249788784274003
2216	21	-1.035992128495728
2216	22	-0.9309700943238468
2216	23	-4.865890751654266
2216	24	-2.2872425509883954
2216	25	-1.4503944212584088
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	11.0
4	20.0
5	56.0
6	69.0
7	364.0
8	216.0
9	608.0
10	1357.0
11	2672.0
12	5204.0
13	8476.0
14	12260.0
15	15605.0
16	18550.0
17	20382.0
18	21627.0
19	22568.0
20	23268.0
21	23987.0
22	25130.0
23	26803.0
24	29335.0
25	32418.0
26	37015.0
27	43228.0
28	51557.0
29	63841.0
30	79589.0
31	99638.0
32	134493.0
33	183784.0
34	254362.0
35	360284.0
36	526612.0
37	822698.0
38	1392755.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.731112714240886	21.30755445319249	14.033111008590732	22.92822182397589
2	15.899795995766002	21.268236305749213	40.2702376393572	22.561730059127587
3	17.94170620176403	26.258870625061643	30.386297920271065	25.413125252903264
4	12.836140912206181	17.829450933163354	38.679278763568355	30.655129391062115
5	13.35255001280267	37.594213700630505	35.63541686112665	13.417819425440184
6	30.15293962596633	39.04167695818387	17.90783900257687	12.897544413272923
7	25.979639462447153	32.87895936463989	23.73842271502713	17.402978457885826
8	23.4831937130544	36.74892548079693	21.580401691575542	18.18747911457313
9	24.31242198922184	17.38538753265621	22.391444026748133	35.91074645137382
10	14.758070313853732	30.122128476152206	34.230836230510874	20.88896497948319
11	31.434414869067528	25.756623007533108	24.21954008713086	18.589422036268502
12	20.88718336274867	27.549871663126073	31.606169386240996	19.956775587884263
13	26.10438074697249	24.373096823921	27.11986884142878	22.402653587677733
14	20.775770995772362	23.834126438328635	28.4035450963618	26.98655746953721
15	22.511532037123757	31.054642128900706	24.591516071105932	21.842309762869604
16	21.69526573294281	31.314520141010227	26.042236245822703	20.947977880224258
17	20.040950403282668	30.352378081994818	28.568574643930578	21.03809687079194
18	20.14605133688361	30.059545070133677	29.44729546936678	20.347108123615936
19	20.934415309568966	29.109328093494984	29.595401746925116	20.360854850010934
20	22.11154015325245	28.719063057367254	28.945921105655348	20.22347568372495
21	22.506887122158666	28.935405395006438	27.985389186624943	20.572318296209957
22	21.53664619086209	28.591293984979906	29.34377346673982	20.52828635741819
23	21.86944769522972	28.81522183182077	28.547063728678744	20.768266744270765
24	21.49110507980624	28.272843759223896	28.939510579502205	21.296540581467664
25	21.97311020030129	28.579948469442822	28.394573147838088	21.0523681824178
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2791.0
1	2791.0
2	3177.0
3	3563.0
4	3563.0
5	3563.0
6	5723.0
7	7883.0
8	7883.0
9	7883.0
10	12869.5
11	17856.0
12	17856.0
13	17856.0
14	30191.0
15	42526.0
16	42526.0
17	42526.0
18	67435.0
19	92344.0
20	92344.0
21	92344.0
22	131950.5
23	171557.0
24	171557.0
25	171557.0
26	221416.5
27	271276.0
28	271276.0
29	271276.0
30	324013.5
31	376751.0
32	376751.0
33	376751.0
34	419689.5
35	462628.0
36	462628.0
37	462628.0
38	489566.5
39	516505.0
40	516505.0
41	516505.0
42	517199.0
43	517893.0
44	517893.0
45	517893.0
46	499443.5
47	480994.0
48	480994.0
49	480994.0
50	458790.0
51	436586.0
52	436586.0
53	436586.0
54	396210.5
55	355835.0
56	355835.0
57	355835.0
58	307073.5
59	258312.0
60	258312.0
61	258312.0
62	212437.5
63	166563.0
64	166563.0
65	166563.0
66	127900.0
67	89237.0
68	89237.0
69	89237.0
70	65377.5
71	41518.0
72	41518.0
73	41518.0
74	29572.5
75	17627.0
76	17627.0
77	17627.0
78	12218.5
79	6810.0
80	6810.0
81	6810.0
82	4696.5
83	2583.0
84	2583.0
85	2583.0
86	1715.5
87	848.0
88	848.0
89	848.0
90	555.0
91	262.0
92	262.0
93	262.0
94	167.0
95	72.0
96	72.0
97	72.0
98	47.0
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08046825938377854
2	0.01695523587359319
3	0.02851981251563637
4	0.03492409997875988
5	0.043839421015554124
6	0.016079829673597886
7	0.02022649062094405
8	0.03897861290505391
9	0.03911683493663211
10	0.022714487189351745
11	0.02149352591041093
12	0.016171977694650026
13	0.024350114563027173
14	0.04095979535767485
15	0.03745817055769365
16	0.023382560341979736
17	0.022069451041986786
18	0.02930307069457953
19	0.017485086994642976
20	0.015734274594652375
21	0.01140331760520194
22	0.02126315585778059
23	0.019927009552524605
24	0.02057204569988956
25	0.10997866312572539
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4340842.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.31763694752311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.60510108558793	68.69311965710205
2	10.183594897259681	16.154773657629846
3	1.8363264258263587	4.369592182825135
4	0.5222854622838231	1.6570579472159026
5	0.2442075507882782	0.9684982926634231
6	0.13858053242700116	0.65951282154236
7	0.09313207837084242	0.5170911466270725
8	0.06675688800551698	0.4235998885254445
9	0.050081664687775404	0.35751233676893024
>10	0.23958520715784198	3.295536215745545
>50	0.012309469956836547	0.6642911772417238
>100	0.0070477167643491385	1.0038992304659615
>500	4.955105375268119E-4	0.27759281270908076
>1k	4.372150114817006E-4	0.6705273476637836
>5k	5.8295334864023924E-5	0.28739528527369823
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	6641	0.15298875195181028	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5790	0.13338426047296814	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4474	0.10306756154681511	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	2.3037005263034223E-5	2.3037005263034223E-5	0.0	2.3037005263034223E-5	0.0
5	2.3037005263034223E-5	2.3037005263034223E-5	0.0	2.3037005263034223E-5	0.0
6	2.3037005263034223E-5	2.3037005263034223E-5	0.0	2.3037005263034223E-5	0.0
7	2.3037005263034223E-5	2.3037005263034223E-5	0.0	2.3037005263034223E-5	0.0
8	2.3037005263034223E-5	2.3037005263034223E-5	0.0	2.3037005263034223E-5	0.0
9	2.3037005263034223E-5	2.3037005263034223E-5	0.0	6.911101578910267E-5	0.0
10	2.3037005263034223E-5	2.3037005263034223E-5	0.0	6.911101578910267E-5	0.0
11	2.3037005263034223E-5	2.3037005263034223E-5	0.0	6.911101578910267E-5	0.0
12	2.3037005263034223E-5	2.3037005263034223E-5	0.0	6.911101578910267E-5	0.0
13	2.3037005263034223E-5	2.3037005263034223E-5	0.0	9.21480210521369E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGTAC	300	0.0	10.451921	3
GGTATCA	3800	0.0	9.931747	1
GTGGTAT	1945	0.0	9.6775255	1
TAATACG	110	6.827678E-5	9.501856	4
TGGTATC	1925	0.0	9.279413	2
ACACCGT	155	4.2738066E-7	9.192378	6
GTCCTAC	270	1.8189894E-12	8.802277	1
ACTGATC	415	0.0	8.697687	8
CTAGACT	485	0.0	8.62024	4
GTCTTAC	455	0.0	8.566258	1
GTACTAC	325	0.0	8.482686	1
CAGGACT	555	0.0	8.389027	4
GTCTTAG	460	0.0	8.266485	1
TCTAGAC	415	0.0	8.242479	3
CAGTACT	445	0.0	8.113944	4
GGTCTAC	235	5.460606E-9	8.090603	1
GTTCTAG	555	0.0	8.050514	1
TACACCG	130	4.179656E-4	8.040031	5
CCCGTGC	155	3.907449E-5	7.9667273	8
GTATCAA	6520	0.0	7.946349	1
>>END_MODULE