FastQCFastQC Report
Wed 25 May 2016
SRR1295352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295352_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4340842
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT166640.38388865570320224No Hit
GGTATCAACGCAGAGTACTTTTTTT135790.31281949446674173No Hit
TATCAACGCAGAGTACTTTTTTTTT97020.22350502506195805No Hit
GTGGTATCAACGCAGAGTACATGGG70660.1627794791885998No Hit
ACGCAGAGTACTTTTTTTTTTTTTT64380.14831223988341433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC655.476133E-513.14891058
AGCGTCA4000.011.646013
GGTATCA52750.011.0058731
CAGCGTC4300.010.8334992
CTAGTAC3000.010.7745423
GTGGTAT22350.010.3477491
TCCGTAC1054.0759463E-59.959663
GCACCGT1054.1231873E-59.9492196
GTTCTAG4450.09.8381841
TGGTATC22750.09.7367862
AGAACCG1502.623874E-79.5069485
ACCGTCT800.00453221439.4964358
GCAGCGT4550.09.4127881
AAACCGA1752.6877387E-89.2256396
TGGACTG6300.09.0542365
ATGCCGT1054.509877E-49.04474456
TATACCG950.00181273169.0065815
ACCGACC950.00183107268.9966238
CGGTATC850.00739476368.9477162
CCTACAC4500.08.8731523
Produced by FastQC (version 0.11.5)