FastQCFastQC Report
Wed 25 May 2016
SRR1295351_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295351_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2606729
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1226814.706319682636745No Hit
GCTTATGTACTCTGCGTTGATACCA748252.870455655344303No Hit
CTTATGTACTCTGCGTTGATACCAC478901.8371683439283484No Hit
GAGTACATAAGCAGTGGTATCAACG322051.2354563899814672No Hit
TATGTACTCTGCGTTGATACCACTG308971.185278561753063No Hit
CCCATGTACTCTGCGTTGATACCAC287931.104564379342847No Hit
GCGTTGATACCACTGCTTATGTACT253640.9730202103862733No Hit
CATAAGCAGTGGTATCAACGCAGAG216720.8313867686284229No Hit
ACTCTGCGTTGATACCACTGCTTAT203730.7815542006859938No Hit
GTACATGATAAGCAGTGGTATCAAC198210.7603782364795113No Hit
ATCATGTACTCTGCGTTGATACCAC197200.756503648825789No Hit
CATGTACTCTGCGTTGATACCACTG192690.7392022722730288No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA182860.7014921765937311No Hit
CTGCTTATGTACTCTGCGTTGATAC178710.6855718411848719No Hit
ACGCAGAGTACATAAGCAGTGGTAT178580.6850731318829076No Hit
GTATCAACGCAGAGTACATAAGCAG169780.6513143483653269No Hit
TATCAACGCAGAGTACATAAGCAGT145630.5586695049619658No Hit
GTACATGGGAAGCAGTGGTATCAAC143580.5508052428925293No Hit
GGTATCAACGCAGAGTACATAAGCA132140.5069188243196742No Hit
AAGCAGTGGTATCAACGCAGAGTAC112490.43153699521507605No Hit
GTATCAACGCAGAGTACTTTTTTTT105320.4040312590990471No Hit
GTGGTATCAACGCAGAGTACATAAG99350.3811289934626883No Hit
GTGGTATCAACGCAGAGTACATGGG91060.34932668489896723No Hit
GCAGTGGTATCAACGCAGAGTACAT90820.3484059908030332No Hit
ACATAAGCAGTGGTATCAACGCAGA86150.33049081818631704No Hit
GTACTCTGCGTTGATACCACTGCTT84220.32308690316484756No Hit
GAGTACATGATAAGCAGTGGTATCA76790.29458374844489016No Hit
CCACTGCTTATGTACTCTGCGTTGA71050.2725638146504681No Hit
GTACTTTTTTTTTTTTTTTTTTTTT69440.2663874917569107No Hit
GGTATCAACGCAGAGTACTTTTTTT68050.2610551384512928No Hit
GATACCACTGCTTATGTACTCTGCG67970.26074824041931477No Hit
GCTTATCATGTACTCTGCGTTGATA65460.2511193146660048No Hit
ATACCACTGCTTATGTACTCTGCGT63860.24498135402644464No Hit
TATCAACGCAGAGTACTTTTTTTTT62610.24018607227678823No Hit
GAGTACATGGGAAGCAGTGGTATCA60960.23385630036724184No Hit
GTTGATACCACTGCTTATGTACTCT60310.23136275385742056No Hit
GCGTTGATACCACTGCTTATCATGT59890.229751539189536No Hit
GTACATGGGATAAGCAGTGGTATCA59750.2292144676335745No Hit
GCTTATCCCATGTACTCTGCGTTGA54980.21091567247688578No Hit
GATAAGCAGTGGTATCAACGCAGAG54600.20945790682499024No Hit
GTACTGGTTCACTATCGGTCAGTCA53840.20654237552119917No Hit
GTATCAACGCAGAGTACATGGGAAG53570.2055065946632734No Hit
CAGTGGTATCAACGCAGAGTACATA51930.19921518500772423No Hit
GCGTTGATACCACTGCTTCCCATGT50890.19522551059201015No Hit
TACCACTGCTTATGTACTCTGCGTT47700.18298795156688707No Hit
TGATACCACTGCTTATGTACTCTGC47690.18294958931288982No Hit
GGTATCAACGCAGAGTACATGGGAA46760.17938189969114549No Hit
ACGCAGAGTACATGATAAGCAGTGG46450.17819266981723073No Hit
CCCCATGTACTCTGCGTTGATACCA45300.17378101060754686No Hit
TATCAACGCAGAGTACATGATAAGC44210.1695995249218465No Hit
TATCAACGCAGAGTACATGGGAAGC42240.16204216088438805No Hit
GTATCAACGCAGAGTACATGATAAG41900.16073784424848153No Hit
GCAGAGTACATAAGCAGTGGTATCA41390.15878136929462172No Hit
ACGCAGAGTACATGGGAAGCAGTGG40170.1541011743069571No Hit
CAGTGGTATCAACGCAGAGTACATG38470.14757959112742444No Hit
GTTAATGATAGTGTGTCGAAACACA38110.1461985499835234No Hit
GGATAAGCAGTGGTATCAACGCAGA37560.14408862601367461No Hit
GAACAAAAAAAAAAAAAAAAAAAAA37220.14278430937776806No Hit
GGTATCAACGCAGAGTACATGATAA36910.14159507950385328No Hit
ATGATAAGCAGTGGTATCAACGCAG36880.14147999274186154No Hit
ACTCTGCGTTGATACCACTGCTTCC36150.13867954820006223No Hit
ATCAACGCAGAGTACATAAGCAGTG35160.13488168505433437No Hit
ATACAGGGTGACAGCCCCGTACACA34110.13085364838462304No Hit
CTGCTTATCATGTACTCTGCGTTGA33580.12882044892276873No Hit
GGTATCAACGCAGAGTACATGGGGG32930.12632690241294742No Hit
GGGATAAGCAGTGGTATCAACGCAG32600.12506094803103812No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32420.1243704274590876No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA32160.12337300885515909No Hit
GGATACCACGTGTCCCGCCCTACTC31470.12072601332934878No Hit
AAAAAGTACTCTGCGTTGATACCAC30760.11800229329554396No Hit
GTATCAACGCAGAGTACATGGGGGT30250.11604581834168416No Hit
GTACACAAGCAGTGGTATCAACGCA30180.1157772825637034No Hit
ACTTAGATGTTTCAGTTCCCCCGGT30120.1155471090397199No Hit
GAGTACATGGGATAAGCAGTGGTAT29950.11489495072176663No Hit
ATGTACTCTGCGTTGATACCACTGC28220.1082582807802422No Hit
CATGATAAGCAGTGGTATCAACGCA28020.1074910357002972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCTA402.765808E-416.62201118
GTCTACT508.7294975E-515.1975599
AGCAGTT4150.014.8765578
GACTGTC902.2973836E-914.7748387
CTAGCTC1400.014.2534563
CATAGAC602.5637084E-514.2534553
TACACCC400.00527188614.2534555
ACAAGTT602.5744022E-514.24716513
GTACAAA9600.014.1623571
TTGTATC4600.014.04068413
TTACACG759.653504E-713.9367114
GTCTAGC1301.8189894E-1213.8957181
AAGTTAC551.9642651E-413.8154337
GCAGTTG4400.013.6000889
AGTTGTA4550.013.36049311
CGGGTCC500.001502273413.2973546
ACTCTAT500.001502273413.2973546
CGGGTCG655.4654454E-513.15122912
CAGTTGT4650.013.07316910
CGCAAAA2850.012.6697392
Produced by FastQC (version 0.11.5)