FastQCFastQC Report
Wed 25 May 2016
SRR1295351_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295351_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2606729
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1334775.120478576791067No Hit
GCTTATGTACTCTGCGTTGATACCA845613.2439505602615384No Hit
CTTATGTACTCTGCGTTGATACCAC569792.1858428705093624No Hit
CCCATGTACTCTGCGTTGATACCAC350321.3439064820316957No Hit
GAGTACATAAGCAGTGGTATCAACG349561.3409909507279045No Hit
GTATCAACGCAGAGTACTTTTTTTT330181.2666449024812323No Hit
GCGTTGATACCACTGCTTATGTACT290901.1159579687800303No Hit
TATGTACTCTGCGTTGATACCACTG281211.0787849446566944No Hit
CATAAGCAGTGGTATCAACGCAGAG267771.027226075284389No Hit
ACTCTGCGTTGATACCACTGCTTAT241030.92464540809574No Hit
ATCATGTACTCTGCGTTGATACCAC225430.8648002918600285No Hit
CATGTACTCTGCGTTGATACCACTG224240.8602351836343556No Hit
ACGCAGAGTACATAAGCAGTGGTAT221730.8506062578810456No Hit
GGTATCAACGCAGAGTACTTTTTTT218410.8378699895539582No Hit
CTGCTTATGTACTCTGCGTTGATAC210810.8087146765160474No Hit
GTACATGATAAGCAGTGGTATCAAC204390.7840861094498124No Hit
TATCAACGCAGAGTACTTTTTTTTT199240.7643295486412282No Hit
GTACTTTTTTTTTTTTTTTTTTTTT191740.7355578581432899No Hit
GTATCAACGCAGAGTACATAAGCAG188940.7248164270240597No Hit
TATCAACGCAGAGTACATAAGCAGT162970.6251896533931989No Hit
GTACATGGGAAGCAGTGGTATCAAC149600.5738993197988744No Hit
GGTATCAACGCAGAGTACATAAGCA145880.559628561311897No Hit
AAGCAGTGGTATCAACGCAGAGTAC132020.5064584772717072No Hit
ACGCAGAGTACTTTTTTTTTTTTTT124690.47833894509172226No Hit
ACATAAGCAGTGGTATCAACGCAGA107640.4129313020264093No Hit
GCAGTGGTATCAACGCAGAGTACAT103760.39804674747547597No Hit
GTGGTATCAACGCAGAGTACATAAG102180.39198551134391035No Hit
GTGGTATCAACGCAGAGTACATGGG95470.36624443891175495No Hit
GTACTCTGCGTTGATACCACTGCTT89230.34230639241747035No Hit
CCACTGCTTATGTACTCTGCGTTGA84330.3235088879588174No Hit
GAGTACATGATAAGCAGTGGTATCA82570.3167571312553012No Hit
GATACCACTGCTTATGTACTCTGCG77920.2989186831465795No Hit
GCTTATCATGTACTCTGCGTTGATA74470.2856837055175279No Hit
ATACCACTGCTTATGTACTCTGCGT69170.2653517108989849No Hit
GCGTTGATACCACTGCTTATCATGT65980.2531141518738618No Hit
GAGTACATGGGAAGCAGTGGTATCA65970.2530757896198646No Hit
CAGTGGTATCAACGCAGAGTACATA64690.24816542110821646No Hit
GAGTACTTTTTTTTTTTTTTTTTTT62240.23876666887888998No Hit
GTACATGGGATAAGCAGTGGTATCA61950.2376541635129697No Hit
GCTTATCCCATGTACTCTGCGTTGA60950.2338179381132446No Hit
GATAAGCAGTGGTATCAACGCAGAG60750.2330506930332996No Hit
GTTGATACCACTGCTTATGTACTCT60320.23140111611141778No Hit
ACGCAGAGTACATGATAAGCAGTGG57890.2220790883900858No Hit
GCGTTGATACCACTGCTTCCCATGT57520.22065968499218755No Hit
GTATCAACGCAGAGTACATGGGAAG57340.219969164420237No Hit
GTACTGGTTCACTATCGGTCAGTCA57320.2198924399122425No Hit
TACCACTGCTTATGTACTCTGCGTT56160.2154424184485614No Hit
CCCCATGTACTCTGCGTTGATACCA55850.21425318857464662No Hit
TGATACCACTGCTTATGTACTCTGC52430.20113329770758678No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA51810.1987548379597572No Hit
GGTATCAACGCAGAGTACATGGGAA49570.190161693064373No Hit
ACGCAGAGTACATGGGAAGCAGTGG49440.18966298376240875No Hit
TATCAACGCAGAGTACATGATAAGC48050.18433063045679088No Hit
GCAGAGTACATAAGCAGTGGTATCA48040.18429226820279362No Hit
GCAGAGTACTTTTTTTTTTTTTTTT45180.17332066355957984No Hit
CAGTGGTATCAACGCAGAGTACATG44960.1724766939716403No Hit
TATCAACGCAGAGTACATGGGAAGC44880.1721697959396623No Hit
GTATCAACGCAGAGTACATGATAAG44460.17055858127177778No Hit
GGATAAGCAGTGGTATCAACGCAGA44110.16921590238187398No Hit
ATGATAAGCAGTGGTATCAACGCAG43220.16580166177611866No Hit
ACTCTGCGTTGATACCACTGCTTCC43200.16572493726812415No Hit
ATCAACGCAGAGTACATAAGCAGTG42130.16162017609041832No Hit
CTTATACACATCTCCGAGCCCACGA41270.15832102224665473No Hit
ATACAGGGTGACAGCCCCGTACACA41190.1580141242146767No Hit
CTGCTTATCATGTACTCTGCGTTGA39840.15283521992504784No Hit
GGGATAAGCAGTGGTATCAACGCAG39710.15233651062308357No Hit
GTTAATGATAGTGTGTCGAAACACA38760.14869209649334472No Hit
GGTATCAACGCAGAGTACATGATAA38460.1475412288734272No Hit
GGATACCACGTGTCCCGCCCTACTC36320.1393317065180155No Hit
GGTATCAACGCAGAGTACATGGGGG35410.13584074140426566No Hit
ACTTAGATGTTTCAGTTCCCCCGGT34630.13284848559248008No Hit
AAAAAGTACTCTGCGTTGATACCAC33920.13012476555867525No Hit
CATGATAAGCAGTGGTATCAACGCA33410.12816829060481547No Hit
TACATAAGCAGTGGTATCAACGCAG32940.12636526466694467No Hit
GAGTACATGGGATAAGCAGTGGTAT32840.12598164212697216No Hit
GTACACAAGCAGTGGTATCAACGCA32630.12517603479302988No Hit
ATCAACGCAGAGTACTTTTTTTTTT32520.12475404999906013No Hit
CCATGTACTCTGCGTTGATACCACT32030.12287429955319482No Hit
GTATCAACGCAGAGTACATGGGGGT31240.119843681487412No Hit
GTGGTATCAACGCAGAGTACTTTTT30260.1160841805956814No Hit
GCTTCCCATGTACTCTGCGTTGATA30190.11581564481770067No Hit
ATGTACTCTGCGTTGATACCACTGC29800.11431951691180786No Hit
TACCACTGCTTATCATGTACTCTGC29130.11174924589399204No Hit
ATCATTAACTGAATCCATAGGTTAA29010.11128889884602503No Hit
GCGTTGATACCACTGCTTATCCCAT27740.10641689258837417No Hit
GTATCAACGCAGAGTACATGGGATA26890.10315610099860785No Hit
ACCCTGTATCGCGCGCCTTTCCAGA26870.10307937649061333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCT307.7418727E-418.99370817
ATCTGAG453.5504418E-516.8832957
TCCTAGT350.002161360616.2962632
GAGTGTG350.002175537216.2803217
CTTATAC5300.015.0837341
CGCAGTA456.7815103E-414.77288313
GTCTAGA1300.014.6417191
GCCTAGT400.005217679314.2756771
CCTAGTC400.005257734614.2592313
CAATCGG400.00529198514.2452829
TTAGACA1101.8371793E-1013.8271334
CTAGCGT551.9664696E-413.81360715
GCAGTTA707.2919593E-613.5669358
CTAGAGG1550.013.4926053
CTTAGAC500.001492720813.3086153
CTTAAAG500.001503770713.29559518
CGATCTA500.001503770713.29559512
GCCGTTA500.001503770713.2955958
ATCGCCG2550.013.03789919
AATCGCC2800.012.88858818
Produced by FastQC (version 0.11.5)