FastQCFastQC Report
Wed 25 May 2016
SRR1295350_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295350_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3635411
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG53840.1480987981826539No Hit
GTATCAACGCAGAGTACTTTTTTTT49970.13745350938312062No Hit
GGTATCAACGCAGAGTACTTTTTTT42300.11635548222745654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34750.010.696781
AAGACCG909.48847E-510.5592515
GTGGTAT15400.09.7536561
CGTAGAC900.00111084729.5031953
CTAGTAC2700.09.1512243
CGTGTAA1054.5035337E-49.04592910
ACTGCGT850.00744932338.9396298
TGGTATC16300.08.8034522
CCCCTAT1752.5997724E-78.6918661
GTCTAAG2751.8189894E-128.6424811
GTCCTAG3000.08.5560561
GCGTCAG1901.00082616E-78.5060211
GTTCTAG4150.08.475881
TTAGTAC2855.456968E-128.3361363
CCTAGAC2909.094947E-128.19240953
GTCTTAG3300.08.0663161
TAAGACC2951.2732926E-118.0536664
TGGACGA1304.1722285E-48.0412755
TAGACTG4050.07.97815
GTCTCGC1553.8662733E-57.9733861
Produced by FastQC (version 0.11.5)