##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295350_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3635411
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.260406044873605	31.0	30.0	33.0	26.0	34.0
2	30.08919954305029	31.0	30.0	34.0	25.0	34.0
3	30.375158407123706	31.0	30.0	34.0	26.0	34.0
4	33.48639369798903	35.0	33.0	37.0	27.0	37.0
5	33.89759177160437	35.0	35.0	37.0	28.0	37.0
6	34.092778505649015	36.0	35.0	37.0	29.0	37.0
7	34.010461540662114	37.0	35.0	37.0	29.0	37.0
8	33.987942766306205	37.0	35.0	37.0	28.0	37.0
9	34.93574619210868	38.0	35.0	39.0	27.0	39.0
10	34.8235035873523	38.0	34.0	39.0	27.0	39.0
11	35.29453753647112	39.0	35.0	39.0	27.0	39.0
12	35.26722288071417	39.0	35.0	39.0	27.0	39.0
13	35.202787800333994	38.0	35.0	39.0	27.0	39.0
14	35.419745112725906	39.0	34.0	41.0	25.0	41.0
15	36.04532417380043	39.0	35.0	41.0	27.0	41.0
16	36.1708909941682	39.0	36.0	41.0	27.0	41.0
17	35.99474749897605	39.0	35.0	41.0	27.0	41.0
18	35.965814319206274	39.0	35.0	41.0	27.0	41.0
19	36.00490288443315	39.0	35.0	41.0	27.0	41.0
20	35.97380461246335	39.0	35.0	41.0	27.0	41.0
21	35.96967358023618	39.0	35.0	41.0	27.0	41.0
22	35.74115581429445	39.0	34.0	41.0	26.0	41.0
23	35.43994062844614	39.0	34.0	41.0	25.0	41.0
24	35.740700570031834	39.0	34.0	41.0	26.0	41.0
25	34.49265048711136	38.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.40568358360091494
1101	2	0.6329476474536335
1101	3	0.43318136977994115
1101	4	0.27510009902121624
1101	5	0.2665781403515268
1101	6	0.20559396118873252
1101	7	0.2894290891296478
1101	8	0.3129433220531723
1101	9	0.1596282500582049
1101	10	0.6089233806846437
1101	11	0.44038909692346095
1101	12	0.493801660536036
1101	13	0.45988428923448055
1101	14	0.9051438352028782
1101	15	0.7368993443045824
1101	16	0.54775771452924
1101	17	0.7618092260239564
1101	18	0.7937768371511496
1101	19	0.7590307181489209
1101	20	0.9178807079257112
1101	21	0.9013933209373945
1101	22	0.6713852289404088
1101	23	-1.4148542214808657
1101	24	0.16367584085789133
1101	25	0.4872831924325638
1102	1	0.12087278727668505
1102	2	0.18210323487468472
1102	3	0.09557285671969495
1102	4	0.15863712746255487
1102	5	-0.031104611943064242
1102	6	-0.013334004374428332
1102	7	-0.1108457507600562
1102	8	-0.06330461267598508
1102	9	0.03783052503347761
1102	10	0.6115503499445722
1102	11	0.3737061291223469
1102	12	0.19765943451943002
1102	13	0.16462467294660854
1102	14	0.6431633302545166
1102	15	0.36688258729094514
1102	16	0.357002253649199
1102	17	0.5693131355333207
1102	18	0.5503659490984987
1102	19	0.627254350373228
1102	20	0.6601557660848414
1102	21	0.5713999242782961
1102	22	0.5872816670043832
1102	23	0.5729624073340105
1102	24	0.5833088304859473
1102	25	0.29895045312362356
1103	1	-0.30501551307911967
1103	2	-1.472392066699328
1103	3	-0.2368744570471506
1103	4	-0.1448715314869986
1103	5	-0.09206340210508301
1103	6	-0.20323735257314013
1103	7	-0.07109830121916616
1103	8	0.025270511157458486
1103	9	0.647724857406196
1103	10	0.6983389449246928
1103	11	0.47540464634096224
1103	12	0.33915960356616637
1103	13	0.12133958501546971
1103	14	-1.0435785756266966
1103	15	-0.19151849890142358
1103	16	-0.06742634154558402
1103	17	0.4037911567741759
1103	18	0.5873426421149901
1103	19	0.584307892573193
1103	20	0.5307808096881672
1103	21	0.641345560994175
1103	22	0.1700582194169229
1103	23	1.0464118730926586
1103	24	0.835077918009226
1103	25	0.5470434449288817
1104	1	0.030819599254648722
1104	2	0.10137724215786648
1104	3	-0.26582504218606573
1104	4	-0.22523927336970928
1104	5	-0.15371542325860332
1104	6	-0.06991837847702698
1104	7	-0.0835641185096847
1104	8	-0.014048170301037999
1104	9	-1.91880775941938
1104	10	-0.5019011633330521
1104	11	-0.5092055230889443
1104	12	-0.14720084782281617
1104	13	0.019261403512892628
1104	14	-1.64020215549359
1104	15	-0.5651383989865764
1104	16	-0.2420090375741637
1104	17	0.13158364216728557
1104	18	0.2458327228811541
1104	19	0.3163508939445947
1104	20	0.46496788146708923
1104	21	0.5161442817497246
1104	22	0.6378076452746342
1104	23	1.0435749240488619
1104	24	0.7272255055210479
1104	25	0.5138325286368755
1105	1	-0.2371194484025665
1105	2	-0.04699310637228393
1105	3	-1.497172109571995
1105	4	-0.696639004575978
1105	5	-0.5533388544006002
1105	6	-0.1760152587235453
1105	7	-0.2974012349454611
1105	8	-0.22299522308794906
1105	9	0.060573843238216796
1105	10	0.3222460821036819
1105	11	-0.2235249439372069
1105	12	-0.18962832146227981
1105	13	-0.17885686143642943
1105	14	0.5579179343333251
1105	15	0.14072139030088948
1105	16	-0.3408675587506167
1105	17	0.14286739703541684
1105	18	-0.040411314062957615
1105	19	0.06312862528540109
1105	20	-0.33010981537995576
1105	21	-0.05033883562783359
1105	22	0.10732803881843722
1105	23	0.31826219012460655
1105	24	0.16094781210907172
1105	25	0.34192777150303755
1106	1	-1.4347757514684218
1106	2	-0.9382673044024799
1106	3	-1.0083510592153928
1106	4	-1.415492010226039
1106	5	-0.898725559763534
1106	6	-0.7915158916544769
1106	7	-0.7132438049537981
1106	8	-0.7014545765721536
1106	9	-0.30659124367400636
1106	10	-1.7535946354238732
1106	11	-1.099231202234776
1106	12	-0.873374655094068
1106	13	-0.914751300906623
1106	14	-0.1954217797671447
1106	15	-0.9862831482770247
1106	16	-1.332887534668366
1106	17	-0.6625912463311607
1106	18	-0.7069217033926662
1106	19	-0.5998972476961697
1106	20	-0.29565923030477137
1106	21	-0.32088639807187036
1106	22	-0.3017963552659779
1106	23	-0.6269224404598503
1106	24	-0.9822690207662887
1106	25	-1.6942996513874533
1107	1	0.145769389115479
1107	2	0.30013829418111726
1107	3	0.06773656255234073
1107	4	0.3294742918963749
1107	5	0.03097105004783174
1107	6	0.13442673816431494
1107	7	0.10855161796366986
1107	8	0.10239186311046211
1107	9	0.6438164824296422
1107	10	0.1299999991454115
1107	11	0.18942663862380726
1107	12	0.07920612873677868
1107	13	0.3110110725897286
1107	14	-0.78039529505687
1107	15	-0.3315348440999415
1107	16	-0.06940066044705873
1107	17	0.18423823876526768
1107	18	0.5113489579600596
1107	19	0.46061627152801776
1107	20	0.4683855350487107
1107	21	0.6105520094939436
1107	22	-0.1141168093312146
1107	23	0.7048366980402463
1107	24	0.6126216523898051
1107	25	0.3006195829967666
1108	1	-0.09243130926676457
1108	2	0.21940670325368217
1108	3	-0.10529833370041075
1108	4	-0.287425066634448
1108	5	-0.2550376775309928
1108	6	-0.19995856075752272
1108	7	-0.12129800008317915
1108	8	-0.21446133679837232
1108	9	0.26932453856809246
1108	10	0.2406127466439969
1108	11	0.0842652051777435
1108	12	-0.0010615528620263603
1108	13	-0.12484213369991437
1108	14	0.14046871802489136
1108	15	0.010740971105242636
1108	16	-0.08451728391319335
1108	17	0.3068708748004809
1108	18	0.2833278123428329
1108	19	0.2752951655523148
1108	20	0.3462061530166949
1108	21	0.28692440766635485
1108	22	0.2867055587739742
1108	23	0.09326627219334682
1108	24	0.22310498724399963
1108	25	-0.6189377898286708
1109	1	-0.7630535194721766
1109	2	-0.6812987669875135
1109	3	-0.7974828326144099
1109	4	-0.5271180658206944
1109	5	-0.7442963139272649
1109	6	-0.9461399826701964
1109	7	-0.7091687697673095
1109	8	-1.204906664303799
1109	9	-0.8250286703688232
1109	10	-1.080526080415666
1109	11	-1.0952250739213838
1109	12	-1.0214156473530451
1109	13	-1.0100594759007535
1109	14	-0.5712062918478367
1109	15	-1.064659585058628
1109	16	-1.2450126519566567
1109	17	-1.2005413401617986
1109	18	-1.9291161673851605
1109	19	-2.1378599500184805
1109	20	-1.782215645974489
1109	21	-1.5717538916210145
1109	22	-1.5227438980762074
1109	23	-1.022541429434753
1109	24	-2.0835284038298525
1109	25	-1.0901639195084982
1110	1	0.07032764733863317
1110	2	0.19900198515944822
1110	3	-0.01173054102006077
1110	4	0.34424349496391926
1110	5	0.284593543684025
1110	6	0.2447483803057935
1110	7	0.09780954318976143
1110	8	0.048039032166336426
1110	9	0.3699183371247017
1110	10	0.18678706443231619
1110	11	0.27450873905824125
1110	12	0.23320449661438403
1110	13	0.28582309435225284
1110	14	-2.2563880075822738
1110	15	-0.5703217082497218
1110	16	-0.11313360955284679
1110	17	-0.18997391070897152
1110	18	-2.6834328859240344
1110	19	-0.4145067209002278
1110	20	-1.8575377145085525
1110	21	-0.12795111851729501
1110	22	-1.8294884674290444
1110	23	-0.5070337608795086
1110	24	0.1344125025511147
1110	25	0.16846274550101015
1111	1	-0.13257782109709026
1111	2	-1.334124679841171
1111	3	-0.41856052146601996
1111	4	0.0382694583008103
1111	5	0.007651583371320214
1111	6	0.15563359579826397
1111	7	-0.011543876811082043
1111	8	0.01391934166327502
1111	9	-1.5621071060829266
1111	10	-0.28231526332463375
1111	11	-0.05429436374704011
1111	12	0.03232234321537675
1111	13	-0.0123048901259466
1111	14	0.8880320562663329
1111	15	0.2644482686306162
1111	16	0.1687335587426375
1111	17	-0.08182643487842967
1111	18	0.11071169254097413
1111	19	-0.1187218430216248
1111	20	0.3181009885301691
1111	21	-0.4397018637036041
1111	22	-0.09818137840263574
1111	23	0.19260708524735293
1111	24	-0.3321954277978847
1111	25	-1.886134546257118
1112	1	0.2334687942755309
1112	2	0.4122213264962795
1112	3	0.11257966953146337
1112	4	0.5213323630679128
1112	5	0.22388997699215452
1112	6	0.23775590335625907
1112	7	0.2673559092099964
1112	8	0.1560036486694827
1112	9	-0.1609967788718265
1112	10	0.6267723457529328
1112	11	0.36926924142094464
1112	12	0.1569726155765565
1112	13	0.2674528890999497
1112	14	0.741606946094258
1112	15	0.4288678640030952
1112	16	0.3617623837125308
1112	17	-3.4037435320048033
1112	18	-0.7360194905327475
1112	19	-1.1039479937828531
1112	20	0.0782100572044584
1112	21	-0.2646378289462419
1112	22	-1.4303156966234951
1112	23	-1.1613824335510188
1112	24	-0.0973150458142058
1112	25	0.2584405831066121
1113	1	-0.17459022271146551
1113	2	0.01600913344538668
1113	3	0.07833725247291667
1113	4	-0.13663301208133305
1113	5	0.10095904134963973
1113	6	-0.0029954834912118145
1113	7	-0.057283693636541955
1113	8	-0.05111598659070893
1113	9	0.48788463758759093
1113	10	0.652119650290409
1113	11	0.17001740629954298
1113	12	0.17358267329182553
1113	13	-0.13989508569326148
1113	14	-1.2507186257782763
1113	15	-1.5081987326926054
1113	16	-0.2335879057258765
1113	17	-0.015443750969382108
1113	18	-0.31115639727021716
1113	19	0.12298308625559429
1113	20	0.5143454079141492
1113	21	-1.4052509620792932
1113	22	-1.8453167634292456
1113	23	-0.47006251467135485
1113	24	-2.096090913159607
1113	25	-0.30052414118337367
1114	1	0.08383394240912878
1114	2	0.1918411139648697
1114	3	-0.012879120439883707
1114	4	0.4175181938555923
1114	5	0.2463701035017749
1114	6	0.3608438022230729
1114	7	0.24542966443395642
1114	8	0.2668745050604997
1114	9	0.6850031440810938
1114	10	0.28123317069857734
1114	11	0.36792441001011866
1114	12	0.32628733470184557
1114	13	0.21510839297093298
1114	14	0.987798883498165
1114	15	0.5147372598862461
1114	16	0.5055499653316389
1114	17	-0.29428767490259133
1114	18	0.058869112933862766
1114	19	-0.3513704619818867
1114	20	-1.581909230304774
1114	21	-0.6332716021535063
1114	22	-1.2972938042455695
1114	23	-2.810583154745565
1114	24	-1.7797498881132228
1114	25	-1.3419782228160244
1115	1	0.501824777927041
1115	2	0.5440463958552115
1115	3	0.46714337767283
1115	4	0.8002164464046189
1115	5	0.6605416001986271
1115	6	0.496169771029507
1115	7	0.5558566368461442
1115	8	0.6595754193188199
1115	9	0.8679486250282764
1115	10	1.1327011571244512
1115	11	0.7224020241789333
1115	12	0.6592103045960656
1115	13	0.6066080541355348
1115	14	1.2095682661966123
1115	15	0.751761178240848
1115	16	0.7016957177054763
1115	17	0.7922699864403953
1115	18	1.0199443519103681
1115	19	0.4245576374262896
1115	20	1.0290849430005693
1115	21	-0.0704733294575064
1115	22	1.0121039705893509
1115	23	1.0870121591330673
1115	24	0.9457069175409885
1115	25	1.071956293162991
1116	1	0.4304116141212688
1116	2	0.525209949725781
1116	3	0.4696208755952114
1116	4	0.9238497519684188
1116	5	0.7258410601701115
1116	6	0.6455873798686156
1116	7	0.5872211992014229
1116	8	0.5177669062391814
1116	9	0.7867373740646642
1116	10	0.9256264667565048
1116	11	0.6766850082033926
1116	12	0.6220667816609193
1116	13	0.15063932161531568
1116	14	1.1128924057143266
1116	15	0.7622869480860501
1116	16	0.8076399564026673
1116	17	0.998545698344131
1116	18	0.9133501305507323
1116	19	1.0079776262198266
1116	20	0.8661118455486303
1116	21	0.40027179849864325
1116	22	0.40024642765384044
1116	23	-0.5496456646578309
1116	24	-0.03769174316578017
1116	25	-0.4330733218752343
1201	1	0.3584463981509174
1201	2	0.6345325497163827
1201	3	0.46936939605532046
1201	4	0.1796208197160638
1201	5	0.22695240762595148
1201	6	0.15994595890852992
1201	7	0.12672078847850798
1201	8	0.1965906161884945
1201	9	0.44774613684781883
1201	10	0.7925196077417453
1201	11	0.4253976366425434
1201	12	0.4560497222518052
1201	13	0.3657185579229889
1201	14	1.0506228659392391
1201	15	0.566099576964973
1201	16	0.46645113750936673
1201	17	0.5471432803909195
1201	18	0.5823748759985818
1201	19	0.8699359007530845
1201	20	0.831171350028626
1201	21	0.7232703025830745
1201	22	0.8040197433253553
1201	23	0.4264568353010816
1201	24	0.6842588562072862
1201	25	0.885772529383587
1202	1	0.4263037138445789
1202	2	0.8600430495199483
1202	3	0.6237536687968088
1202	4	0.4191300813526766
1202	5	0.2458431701446102
1202	6	0.17868146243231564
1202	7	0.26989681222208617
1202	8	0.32826007609917696
1202	9	0.8610121445627286
1202	10	0.10713262757347763
1202	11	0.41722643907235124
1202	12	0.40357333256699945
1202	13	0.39748037130900116
1202	14	1.0981126281897104
1202	15	0.7432635833206547
1202	16	0.6773111572826664
1202	17	-1.2947072506276172
1202	18	0.05181653773633599
1202	19	0.5859316356458919
1202	20	0.7828784458632754
1202	21	0.7470622355864975
1202	22	0.9168541219693083
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2210	20	0.9276343849198483
2210	21	1.052892486383385
2210	22	1.163000722459465
2210	23	1.4850600272771146
2210	24	1.3375028798542274
2210	25	1.051800890915274
2211	1	0.14807650526633864
2211	2	-0.6014402903393936
2211	3	-0.0628624641820359
2211	4	0.39808962070654985
2211	5	0.2798358212886356
2211	6	0.21609097506792807
2211	7	0.16569941623958329
2211	8	0.36496819688725424
2211	9	0.6992198354364447
2211	10	0.7997651930801126
2211	11	0.3942155441926758
2211	12	0.26103348752564415
2211	13	0.21605212213179215
2211	14	-0.7788107873681582
2211	15	0.06386859679126644
2211	16	0.16683412840252743
2211	17	0.3169851062128899
2211	18	0.22226334644922474
2211	19	0.547372958312387
2211	20	0.597459526555582
2211	21	0.5872104228517472
2211	22	0.00414279530573225
2211	23	0.5758564900298921
2211	24	-0.10112171105838286
2211	25	0.4555004993253391
2212	1	0.024557363619848616
2212	2	-1.1010256632446094
2212	3	-0.31978175684585963
2212	4	0.22151254254595187
2212	5	0.23341690926310577
2212	6	0.16271980626285654
2212	7	0.1818979924630355
2212	8	0.1978499866620993
2212	9	-2.137700041087463
2212	10	0.10170740336749873
2212	11	0.049656929171248976
2212	12	0.08538620794140428
2212	13	0.08426160142646211
2212	14	0.7648573601673192
2212	15	0.37681425539422264
2212	16	0.4986854497428652
2212	17	0.4965245911730136
2212	18	0.24761034656970082
2212	19	-0.3229738280885357
2212	20	0.4087087983685791
2212	21	0.4471957003206555
2212	22	-1.6399737952514002
2212	23	-0.2885317727987058
2212	24	0.3020532776917122
2212	25	-0.2965227473399068
2213	1	0.1341883404974773
2213	2	0.39302294242122215
2213	3	0.18220458766936787
2213	4	0.5201769501083788
2213	5	0.35384413094359246
2213	6	0.27847541902399087
2213	7	0.2384811427928497
2213	8	0.2988572996167562
2213	9	0.7159115157726959
2213	10	0.8140792097183223
2213	11	0.08944449819499312
2213	12	0.129007628083869
2213	13	0.142105982858439
2213	14	-1.5051859938532388
2213	15	-0.2741591962796548
2213	16	0.02378304610709847
2213	17	-2.0745942523667154
2213	18	-0.6946822069036216
2213	19	-0.4409183603990172
2213	20	0.2973451613252962
2213	21	-0.15534754271384799
2213	22	0.48954884564523127
2213	23	0.8616426472553229
2213	24	0.6797598183873248
2213	25	-0.8214636447178094
2214	1	0.3393878767961418
2214	2	0.2566365941011419
2214	3	0.2371680538461618
2214	4	0.6927019415664759
2214	5	0.5703571452667404
2214	6	0.583098465001683
2214	7	0.5734768655294289
2214	8	0.4513350890111312
2214	9	0.9429504388033507
2214	10	1.00871971958248
2214	11	0.23932653290454908
2214	12	0.22968772426349204
2214	13	0.29867684157106567
2214	14	1.160397763895105
2214	15	0.8054306754901575
2214	16	0.7464860320582432
2214	17	0.734905990235255
2214	18	0.6187445728187058
2214	19	-0.5554044821178294
2214	20	0.42450575080489017
2214	21	0.8233686297274474
2214	22	0.9930465002270665
2214	23	1.3914939058684936
2214	24	1.2001547167157725
2214	25	1.1880917497971097
2215	1	-0.11656969485222746
2215	2	0.2030493512920586
2215	3	0.24033557672930783
2215	4	0.4711571010313662
2215	5	0.44263059560184104
2215	6	0.345034470425837
2215	7	0.3817496117677308
2215	8	0.31124785924087917
2215	9	0.4301961164313255
2215	10	0.7952993737927159
2215	11	0.4569340380548894
2215	12	0.30074647722982917
2215	13	0.31360617111444355
2215	14	1.0573794052229815
2215	15	0.5321039484971735
2215	16	0.44380107625988074
2215	17	0.6725338359598183
2215	18	0.546827473697526
2215	19	-0.104456168038503
2215	20	-1.3419857609170194
2215	21	-1.6837962071567958
2215	22	-0.03843888324490763
2215	23	0.5501249591451511
2215	24	0.7366908212350296
2215	25	0.015466642226769522
2216	1	0.027295417635713193
2216	2	-0.22965139106128873
2216	3	0.017043608486964956
2216	4	0.4803739414640873
2216	5	0.3932740028953674
2216	6	0.2422510838707339
2216	7	0.34838799953514865
2216	8	0.2628412879434876
2216	9	0.6347333450132169
2216	10	-1.2350567907321874
2216	11	-0.24292386241095443
2216	12	0.08676294416440555
2216	13	0.1803784816956906
2216	14	-0.1903942827616234
2216	15	-0.16710035601730766
2216	16	0.17766064302266926
2216	17	0.5048500145166912
2216	18	0.34555038347502176
2216	19	-1.0133093894599625
2216	20	-0.8237465157546993
2216	21	-0.6233470292138179
2216	22	-0.5170791746147287
2216	23	-4.584295293327212
2216	24	-1.892380901757285
2216	25	-0.9325836097813038
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	20.0
4	32.0
5	72.0
6	97.0
7	477.0
8	386.0
9	1093.0
10	2163.0
11	4231.0
12	6907.0
13	10685.0
14	15162.0
15	18720.0
16	21987.0
17	23818.0
18	24679.0
19	25603.0
20	26079.0
21	26820.0
22	27617.0
23	28991.0
24	31347.0
25	34233.0
26	38495.0
27	44342.0
28	50846.0
29	62239.0
30	75802.0
31	93660.0
32	123520.0
33	163627.0
34	220475.0
35	303628.0
36	425854.0
37	634042.0
38	1067661.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3027164197019	21.671013532821178	14.197404433638303	22.828865613838616
2	15.482422389247668	21.620795502473513	40.63024027508117	22.26654183319765
3	17.599820596708554	27.11127681762775	30.70867051705019	24.5802320686135
4	12.95518405766433	18.31407675900668	38.607681844536465	30.12305733879252
5	13.261149136671028	38.151542229929156	35.29943718012685	13.287871453272961
6	29.969564688655737	39.229856209524364	17.948084435290433	12.852494666529465
7	25.173038466321746	33.68114367723112	24.064700813217506	17.081117043229625
8	23.39492637564766	37.19923100885782	21.634232764570488	17.771609850924033
9	24.282549589415055	17.77100807960725	22.61800189331396	35.32844043766374
10	15.067405358034645	30.495149758347768	33.81152914341059	20.625915740206995
11	31.1948359972375	26.3162731572561	24.254496628044873	18.234394217461528
12	20.707110031731165	28.097695562946868	31.647827805943823	19.547366599378144
13	25.861675680360786	24.98340113667475	27.322870936098848	21.832052246865615
14	20.826939588745542	24.201680903526928	28.39185857073665	26.579520936990885
15	22.385182547455514	31.506047122673664	24.61082199535097	21.497948334519855
16	21.612255993942224	31.689557597316266	26.11926910445264	20.578917304288872
17	19.896950531467482	30.71521867195176	28.816238078060824	20.57159271851993
18	19.91212293983107	30.48898675019977	29.6047128256041	19.994177484365057
19	20.770978513037008	29.350988946165934	29.82854121085926	20.049491329937798
20	22.004136835832327	28.994690565450103	29.02377167395483	19.97740092476274
21	22.48166687988247	29.326361864358987	28.042004888832032	20.149966366926513
22	21.379185677727406	28.901251256360215	29.496490170468785	20.223072895443593
23	21.613146590860318	29.157710885937192	28.8788848278344	20.350257695368086
24	21.35961784392836	28.38246752817966	29.16895031442803	21.088964313463947
25	21.892999197006564	28.71340404228438	28.593313014331617	20.800283746377442
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3583.0
1	3583.0
2	4051.0
3	4519.0
4	4519.0
5	4519.0
6	6435.5
7	8352.0
8	8352.0
9	8352.0
10	13039.5
11	17727.0
12	17727.0
13	17727.0
14	28907.0
15	40087.0
16	40087.0
17	40087.0
18	62005.0
19	83923.0
20	83923.0
21	83923.0
22	118024.0
23	152125.0
24	152125.0
25	152125.0
26	193919.5
27	235714.0
28	235714.0
29	235714.0
30	279942.5
31	324171.0
32	324171.0
33	324171.0
34	360071.0
35	395971.0
36	395971.0
37	395971.0
38	418740.5
39	441510.0
40	441510.0
41	441510.0
42	438011.5
43	434513.0
44	434513.0
45	434513.0
46	415565.5
47	396618.0
48	396618.0
49	396618.0
50	374488.5
51	352359.0
52	352359.0
53	352359.0
54	317251.0
55	282143.0
56	282143.0
57	282143.0
58	242748.0
59	203353.0
60	203353.0
61	203353.0
62	167499.5
63	131646.0
64	131646.0
65	131646.0
66	101303.0
67	70960.0
68	70960.0
69	70960.0
70	51927.5
71	32895.0
72	32895.0
73	32895.0
74	23458.5
75	14022.0
76	14022.0
77	14022.0
78	9873.5
79	5725.0
80	5725.0
81	5725.0
82	3977.0
83	2229.0
84	2229.0
85	2229.0
86	1534.5
87	840.0
88	840.0
89	840.0
90	577.5
91	315.0
92	315.0
93	315.0
94	198.0
95	81.0
96	81.0
97	81.0
98	55.5
99	30.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08001846283680167
2	0.01931005875264172
3	0.03138572227459289
4	0.03889518956728689
5	0.04797256761340052
6	0.02247338746568132
7	0.02637941074613022
8	0.042883734466336816
9	0.04321382094074095
10	0.02904760974756362
11	0.028084857530551566
12	0.022390865847080287
13	0.030973114181587724
14	0.0454143974367685
15	0.04219605431132821
16	0.02957024666537016
17	0.028607494448358105
18	0.03419145730702801
19	0.02296851717728752
20	0.020602897444057907
21	0.016394294895405222
22	0.02486651440511128
23	0.024398891899705427
24	0.024068805425301293
25	0.110001317595177
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3635411.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.25789721895194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.64086938559431	70.33871887328621
2	9.308895316798203	14.942247271151468
3	1.6759720441921102	4.035299761938216
4	0.5386430318332003	1.7292142834629463
5	0.23822949406729893	0.95598991246881
6	0.14167958604964084	0.682254339311744
7	0.09405207399898673	0.5283895181767959
8	0.0652428321129076	0.41890020111928605
9	0.047912541183762834	0.3460823825242716
>10	0.22853922635315202	3.0881386634829138
>50	0.012272077098030918	0.6723113277918633
>100	0.006591632281917371	0.9484662396007436
>500	6.535753365581209E-4	0.3697219761961157
>1k	4.1278439996865555E-4	0.7956255365029875
>5k	3.439869999738783E-5	0.14863971298560247
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	5384	0.1480987981826539	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4997	0.13745350938312062	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4230	0.11635548222745654	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.7507206200344335E-5	0.0
10	0.0	2.7507206200344335E-5	0.0	1.3753603100172168E-4	0.0
11	0.0	2.7507206200344335E-5	0.0	1.3753603100172168E-4	0.0
12	0.0	2.7507206200344335E-5	0.0	1.3753603100172168E-4	0.0
13	0.0	2.7507206200344335E-5	0.0	1.65043237202066E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3475	0.0	10.69678	1
AAGACCG	90	9.48847E-5	10.559251	5
GTGGTAT	1540	0.0	9.753656	1
CGTAGAC	90	0.0011108472	9.503195	3
CTAGTAC	270	0.0	9.151224	3
CGTGTAA	105	4.5035337E-4	9.045929	10
ACTGCGT	85	0.0074493233	8.939629	8
TGGTATC	1630	0.0	8.803452	2
CCCCTAT	175	2.5997724E-7	8.691866	1
GTCTAAG	275	1.8189894E-12	8.642481	1
GTCCTAG	300	0.0	8.556056	1
GCGTCAG	190	1.00082616E-7	8.506021	1
GTTCTAG	415	0.0	8.47588	1
TTAGTAC	285	5.456968E-12	8.336136	3
CCTAGAC	290	9.094947E-12	8.1924095	3
GTCTTAG	330	0.0	8.066316	1
TAAGACC	295	1.2732926E-11	8.053666	4
TGGACGA	130	4.1722285E-4	8.041275	5
TAGACTG	405	0.0	7.9781	5
GTCTCGC	155	3.8662733E-5	7.973386	1
>>END_MODULE