FastQCFastQC Report
Wed 25 May 2016
SRR1295350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295350_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3635411
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT146010.4016327177312276No Hit
GGTATCAACGCAGAGTACTTTTTTT122610.33726585522242186No Hit
TATCAACGCAGAGTACTTTTTTTTT84990.2337837454967265No Hit
GTGGTATCAACGCAGAGTACATGGG59710.16424552822225602No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55950.15390281869092656No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGT250.00600765219.0141815
AGCGTCA2850.014.0104493
CAGCGTC3350.012.2031322
GGTATCA44350.011.8681161
ATTCCGA752.0815896E-411.396276
TGGTATC16500.011.1204162
GCAGCGT3300.010.9602371
GTGGTAT18100.010.6749951
TAACACG1355.6334102E-810.5634354
AGGACCG1002.3811112E-510.45785
CTAGGAC4000.09.5070913
GGACCGA800.00453011749.4968926
ACCGAGT800.00453217339.4963698
ATTAGAC2750.09.3342343
TAGACTG4000.09.2694135
TATTCCG1356.788674E-69.1549775
TCCCGTG1252.7686274E-59.1165147
TAACCCG1054.4617287E-49.0543735
ATACCGT1054.509858E-49.0446596
GCGTCAG4450.08.9729844
Produced by FastQC (version 0.11.5)