FastQCFastQC Report
Wed 25 May 2016
SRR1295349_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295349_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2269276
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG37830.16670515177527986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9000.011.9384871
GACCGTG658.032326E-411.69119557
GTCTTAC1605.366019E-1010.6970961
TGGTATC9250.010.6824212
GGTATCA18850.010.4921711
CTAGTCC1201.5210771E-610.2934113
CCCGTGT750.00265046510.1323698
GTACTAG1451.5967817E-79.83641051
GGTCTAG1106.771588E-59.5085311
GTCTCGC1606.546361E-89.508531
CCTATAC1606.620394E-89.501613
GTCTAGT2054.1654857E-109.2766151
GTTCTAC1606.6555003E-78.9142471
GCCCTAG1201.7470414E-48.7161521
TAAGACA3300.08.6378274
GTTTTAG3350.08.5151011
GTGTAAG2803.6379788E-128.48975851
GTATTAA3600.08.4520261
TACCCTG2252.4392648E-98.4458755
TCGCCCA1804.0456507E-78.44289612
Produced by FastQC (version 0.11.5)