##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295349_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2269276
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.599575371175654	31.0	30.0	33.0	27.0	34.0
2	30.448593736504506	31.0	30.0	34.0	26.0	34.0
3	30.929179614996148	31.0	31.0	34.0	27.0	34.0
4	34.14691161410071	35.0	35.0	37.0	30.0	37.0
5	34.39109478089047	37.0	35.0	37.0	30.0	37.0
6	34.538444420158676	37.0	35.0	37.0	31.0	37.0
7	34.586741762570966	37.0	35.0	37.0	31.0	37.0
8	34.657143071182176	37.0	35.0	37.0	31.0	37.0
9	35.69806581482376	39.0	35.0	39.0	29.0	39.0
10	35.65340090848359	39.0	35.0	39.0	29.0	39.0
11	36.01985435002177	39.0	35.0	39.0	30.0	39.0
12	36.04125985556627	39.0	35.0	39.0	30.0	39.0
13	35.94706020774908	39.0	35.0	39.0	30.0	39.0
14	36.24429157140868	39.0	36.0	41.0	27.0	41.0
15	36.85083215968441	40.0	36.0	41.0	30.0	41.0
16	36.95029604155687	40.0	36.0	41.0	30.0	41.0
17	36.748940631285045	40.0	36.0	41.0	29.0	41.0
18	36.718940314003234	40.0	36.0	41.0	29.0	41.0
19	36.791085350569965	40.0	36.0	41.0	29.0	41.0
20	36.78185333119462	40.0	36.0	41.0	29.0	41.0
21	36.772546838727415	40.0	36.0	41.0	29.0	41.0
22	36.53721495313924	39.0	36.0	41.0	27.0	41.0
23	36.219694739643835	39.0	35.0	41.0	27.0	41.0
24	36.55013449223453	39.0	36.0	41.0	27.0	41.0
25	35.41426604784962	39.0	34.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5161425281456964
1101	2	0.7170652318943382
1101	3	0.5365662173913108
1101	4	0.4913184929471157
1101	5	0.4224531586414102
1101	6	0.3022565095881049
1101	7	0.3464394067872334
1101	8	0.31202561925170613
1101	9	0.3087803426111577
1101	10	0.6746331311493137
1101	11	0.49728030546328483
1101	12	0.4865426553116592
1101	13	0.4213661459171547
1101	14	0.955994732733906
1101	15	0.7509274310797451
1101	16	0.5472454146648076
1101	17	0.7954986461285927
1101	18	0.8760392957427285
1101	19	0.902238091139651
1101	20	0.9452572694420383
1101	21	1.024604589838546
1101	22	0.7628755377913734
1101	23	-1.256028749105937
1101	24	0.296247885799545
1101	25	0.8082541990178598
1102	1	0.37377812386941045
1102	2	0.43981638999858674
1102	3	0.3444513983923123
1102	4	0.43807824391053884
1102	5	0.24118066082682788
1102	6	0.1717544902812307
1102	7	0.2314872154653358
1102	8	0.276614876524107
1102	9	0.3607227933048165
1102	10	1.019240007090282
1102	11	0.6733508613157468
1102	12	0.5955864291342579
1102	13	0.5739130732859934
1102	14	1.1387696279351545
1102	15	0.8537005674207307
1102	16	0.5925472659028088
1102	17	0.8989122631645827
1102	18	0.8272718884756358
1102	19	0.9701676796039322
1102	20	0.9778041517119007
1102	21	0.9592055051975308
1102	22	0.7128406988285647
1102	23	0.833534670208607
1102	24	0.8424184185763579
1102	25	0.7262411759354492
1103	1	-0.1524790350300833
1103	2	-1.52763335733437
1103	3	-0.20429425011975866
1103	4	0.13303613273703974
1103	5	-0.2013190584320128
1103	6	0.08733899336938578
1103	7	0.16861467195045066
1103	8	0.37325721229053244
1103	9	1.0151978073418704
1103	10	1.0049603888982546
1103	11	0.7634238540164731
1103	12	0.5509957497406575
1103	13	0.3927676493668457
1103	14	-0.6697154927835456
1103	15	0.1810133130692435
1103	16	0.23503575551539058
1103	17	0.7016298375609864
1103	18	0.9014459479927552
1103	19	0.6949515089133129
1103	20	0.8740736632222905
1103	21	0.9550101093525001
1103	22	0.5538783181435534
1103	23	1.2865094035045104
1103	24	1.0595076942641768
1103	25	0.9197504403936207
1104	1	0.1487534064414895
1104	2	0.21813327217138934
1104	3	-0.07264672132005856
1104	4	0.010156162465769114
1104	5	0.0704524019916235
1104	6	0.011970728783730067
1104	7	0.13102121227003494
1104	8	0.143716149488597
1104	9	-2.1602741361735482
1104	10	-0.42268658248784874
1104	11	-0.19595277177319304
1104	12	0.20576665275068962
1104	13	0.3323142863047863
1104	14	-1.4427851694836562
1104	15	-0.1809930042849004
1104	16	0.16582189449717788
1104	17	0.5780549583339365
1104	18	0.6029578394829116
1104	19	0.5819860685565672
1104	20	0.7619253912044499
1104	21	0.7308200313146003
1104	22	0.9496167053601994
1104	23	1.2884731716204527
1104	24	1.0278620235098046
1104	25	0.9169949591598794
1105	1	-0.016228971638273748
1105	2	0.09297999874317497
1105	3	-1.1488917467196522
1105	4	-0.46954840888255944
1105	5	-0.3419499336999863
1105	6	-0.019280402218541326
1105	7	-0.04033449934573241
1105	8	0.09224432646493597
1105	9	0.5621049786841681
1105	10	0.7245757043589407
1105	11	-0.026805048037417123
1105	12	0.10925895252528761
1105	13	-0.07123456358352342
1105	14	0.7687970357427645
1105	15	0.389356965820447
1105	16	0.037622786704154976
1105	17	0.5484143745702781
1105	18	0.5187206996121319
1105	19	0.3745153732778945
1105	20	-0.2263586919562428
1105	21	0.1995796674539747
1105	22	0.3534406150016238
1105	23	0.5285843210951597
1105	24	0.5170873720032745
1105	25	0.8915090443907658
1106	1	-1.2386970255086602
1106	2	-0.7577491177954094
1106	3	-0.8086600732217377
1106	4	-1.0154028549853322
1106	5	-0.6753827016292107
1106	6	-0.6210589018949619
1106	7	-0.30140888104378405
1106	8	-0.42622161507103584
1106	9	0.11298783490389042
1106	10	-1.3796670227780155
1106	11	-0.7370071156653069
1106	12	-0.3500876879942467
1106	13	-0.4842005290345526
1106	14	-0.016688181332781937
1106	15	-0.5008418610725798
1106	16	-0.8950549485688057
1106	17	-0.352702409996013
1106	18	-0.24283969470330646
1106	19	-0.19445966623076316
1106	20	0.11618942368333052
1106	21	0.07623085603253088
1106	22	-0.09813070215211184
1106	23	-0.14657202972024663
1106	24	-0.3969182641422435
1106	25	-1.1729431201175302
1107	1	-0.10188562843667626
1107	2	0.1183336756326625
1107	3	0.0028595960340851434
1107	4	0.06715701185792256
1107	5	-0.21986850898146315
1107	6	0.026723608754004147
1107	7	-0.08813258079680253
1107	8	-0.18877575474243713
1107	9	0.41717582931989483
1107	10	-0.0021604902226215472
1107	11	-0.013719003126382745
1107	12	-0.11245479970988725
1107	13	0.0520533636525613
1107	14	-0.907001207069257
1107	15	-0.48776690671098066
1107	16	-0.26212907964944776
1107	17	0.027838628769778495
1107	18	0.3168964974433095
1107	19	0.28572073968254585
1107	20	0.1307659709145952
1107	21	0.2653617577041487
1107	22	-0.4180227807575463
1107	23	0.46999291998802306
1107	24	0.29691428767076644
1107	25	-0.13563417499099728
1108	1	-0.37360114146552803
1108	2	-0.02721191114870436
1108	3	-0.3194397437161314
1108	4	-0.5239537646458245
1108	5	-0.5582419762587563
1108	6	-0.5243423366759572
1108	7	-0.3387780573471346
1108	8	-0.4533611951551819
1108	9	0.13562020810553577
1108	10	0.08863010491130296
1108	11	-0.16289227039685983
1108	12	-0.19171716092674984
1108	13	-0.559808120261664
1108	14	-0.29017194016243053
1108	15	-0.45926224177922137
1108	16	-0.3738061822779457
1108	17	-0.15744979015359206
1108	18	-0.11036409098584699
1108	19	0.035840061932162826
1108	20	0.052715298732593396
1108	21	-0.011563592023684066
1108	22	0.042259672053347686
1108	23	-0.20623723558546203
1108	24	-0.08110371294289109
1108	25	-1.1323727001679913
1109	1	-0.8895049636723869
1109	2	-0.6716666633441193
1109	3	-0.7284866656683171
1109	4	-0.6514662185291868
1109	5	-0.8481274625909521
1109	6	-1.0225149261267106
1109	7	-0.7307259380844897
1109	8	-1.0727102582647134
1109	9	-0.8600375349938645
1109	10	-0.9077542234663838
1109	11	-1.1218723192438063
1109	12	-1.1019813415920154
1109	13	-1.136695226512181
1109	14	-0.4850436777785845
1109	15	-1.2629055790313686
1109	16	-1.3435590069851528
1109	17	-1.273984295921906
1109	18	-1.97255793483221
1109	19	-2.2185719390352645
1109	20	-1.9459446291975695
1109	21	-1.7168891097682533
1109	22	-1.6399476450127537
1109	23	-1.1934479717058153
1109	24	-2.0996934792118154
1109	25	-1.2789535589161005
1110	1	-0.008519256246774631
1110	2	0.11319721079031453
1110	3	-0.07885231950135818
1110	4	0.25003965209350554
1110	5	0.15475333975803096
1110	6	0.1459292397243388
1110	7	0.013754943443665013
1110	8	0.06515263712712738
1110	9	0.36668096470738476
1110	10	0.05139980569061464
1110	11	0.22946709182441793
1110	12	0.13359202927811253
1110	13	0.08831314961823011
1110	14	-2.662491762265695
1110	15	-0.9058423933158792
1110	16	-0.23508390260427348
1110	17	-0.26229173106646186
1110	18	-2.8067672244557116
1110	19	-0.6536859973712552
1110	20	-1.8125346826801731
1110	21	-0.2256796845137643
1110	22	-1.7037848291666506
1110	23	-0.6199219690445119
1110	24	0.105086377019326
1110	25	0.0644161015298721
1111	1	-0.15249772278878027
1111	2	-1.3290518237906639
1111	3	-0.38481406488145
1111	4	-0.019339589569419502
1111	5	-0.07200337464855977
1111	6	-0.00504558103330055
1111	7	-0.10364892380381718
1111	8	-0.10358881860984326
1111	9	-1.4999868162391294
1111	10	-0.3035486066347133
1111	11	-0.18593559846497953
1111	12	-0.15728522235065157
1111	13	-0.07510170024997365
1111	14	0.7901079570750653
1111	15	0.090450467284235
1111	16	0.09083215730220218
1111	17	-0.19654810596591687
1111	18	0.01633123957916638
1111	19	-0.22567223601517128
1111	20	0.14716181534720363
1111	21	-0.5918768215109864
1111	22	-0.29383890656035305
1111	23	-0.09682357465212732
1111	24	-0.5242329721019559
1111	25	-2.0489778904124947
1112	1	0.19134642314221395
1112	2	0.381685680192124
1112	3	0.045856105197533026
1112	4	0.4053812268649537
1112	5	0.2151010055666589
1112	6	0.27851681824574825
1112	7	0.2209999938138978
1112	8	0.10825451104796002
1112	9	-0.3586441907464817
1112	10	0.5470223096895097
1112	11	0.1604375264596456
1112	12	0.043605482525435946
1112	13	0.050946596297819724
1112	14	0.5630973734426092
1112	15	0.2746586606983783
1112	16	0.33014210135755206
1112	17	-3.5665711348863383
1112	18	-0.8579685888480384
1112	19	-1.0840170320001263
1112	20	-0.06354791347222033
1112	21	-0.5046974915285176
1112	22	-1.616579397020807
1112	23	-1.3695186894182356
1112	24	-0.27400134450904545
1112	25	0.033276600888989094
1113	1	-0.29243040902129636
1113	2	0.0405893416134262
1113	3	-0.09796020169806141
1113	4	-0.33762277011309294
1113	5	-0.04181880304331287
1113	6	-0.014516558780989897
1113	7	-0.23731606663367444
1113	8	-0.2438211409631208
1113	9	0.2646431030819869
1113	10	0.3509618190749819
1113	11	-0.04122217724309962
1113	12	0.041740871816458025
1113	13	-0.32993334154131304
1113	14	-1.458322296338558
1113	15	-1.47245854309584
1113	16	-0.3910821319092719
1113	17	-0.2143329778440588
1113	18	-0.4750660552762156
1113	19	-0.08071096937865008
1113	20	0.28411922456665906
1113	21	-1.3756908815556628
1113	22	-1.9033463217583773
1113	23	-0.7229514198686644
1113	24	-2.2919055246550215
1113	25	-0.6043337357224274
1114	1	-0.07999981782825571
1114	2	-0.03765169388825029
1114	3	-0.24437039204304156
1114	4	0.14112477311857674
1114	5	-0.0835853907101054
1114	6	-0.008473529756876985
1114	7	-0.05428102348185604
1114	8	-0.2215690838078146
1114	9	0.09364372273059729
1114	10	0.022242632042605237
1114	11	-0.07236367219328343
1114	12	-0.09460602635603976
1114	13	-0.06780629299223762
1114	14	0.6239857776189552
1114	15	0.09507194070563685
1114	16	0.03830525352138636
1114	17	-0.6875951758008512
1114	18	-0.4342705343490607
1114	19	-0.7524530053324625
1114	20	-1.9527054347508184
1114	21	-0.9950710483994172
1114	22	-1.8437989420628327
1114	23	-3.2030512592426845
1114	24	-2.3036789192292275
1114	25	-1.7004288138102197
1115	1	0.1921567959136894
1115	2	0.16909788980854756
1115	3	0.09690958980045039
1115	4	0.2962510269730103
1115	5	0.3134262351722725
1115	6	0.22449196681409234
1115	7	0.2631393779305924
1115	8	0.27924316582915765
1115	9	0.4800916277300189
1115	10	0.7380001595004231
1115	11	0.3095461164110205
1115	12	0.26969948576692104
1115	13	0.2594825228240225
1115	14	0.8883269790611976
1115	15	0.4164738957064813
1115	16	0.290974084069596
1115	17	0.31106257662095516
1115	18	0.49724480931512005
1115	19	0.008905712462329518
1115	20	0.5882619190507441
1115	21	-0.4333846876387284
1115	22	0.5463055300456432
1115	23	0.6661377125266554
1115	24	0.605523236798355
1115	25	0.5889221978635959
1116	1	0.28507816918702034
1116	2	0.40600054389664564
1116	3	0.13669146680824795
1116	4	0.6166038180573352
1116	5	0.4570789357725502
1116	6	0.3216063642565814
1116	7	0.212743486763749
1116	8	0.15822648827236208
1116	9	0.42979366833367294
1116	10	0.346135669935677
1116	11	0.20389981940828505
1116	12	0.3582549207059458
1116	13	-0.26948023899912954
1116	14	0.6948207286759995
1116	15	0.38656385870314125
1116	16	0.3049404391299291
1116	17	0.47028599572288954
1116	18	0.5138227744791237
1116	19	0.6739325812740233
1116	20	0.460908904390422
1116	21	-0.02653887439151248
1116	22	0.13704418673928842
1116	23	-0.8741756085179375
1116	24	-0.5393846791919188
1116	25	-1.0546111371151667
1201	1	0.5335489932871873
1201	2	0.750420511522826
1201	3	0.6800411815534773
1201	4	0.40359566711197914
1201	5	0.4732703618069891
1201	6	0.2154771948512959
1201	7	0.35165105922832396
1201	8	0.4058464261354473
1201	9	0.6801372306767917
1201	10	0.9696602299748065
1201	11	0.670643106457085
1201	12	0.618600876569829
1201	13	0.5586985899047079
1201	14	1.2435113136807203
1201	15	0.7897825025596532
1201	16	0.715999781025694
1201	17	0.7982544686315549
1201	18	0.6734035133680862
1201	19	0.9831470053942937
1201	20	0.9141104839277432
1201	21	1.002450038352933
1201	22	0.9816806751640499
1201	23	0.5796899776928655
1201	24	0.9436245133184755
1201	25	1.0289960699987901
1202	1	0.3968074036364406
1202	2	0.7681021049592971
1202	3	0.5335613977386942
1202	4	0.402527987306037
1202	5	0.2103659129780624
1202	6	0.25644634044027015
1202	7	0.34356601513741936
1202	8	0.3799051786299401
1202	9	0.9003655708546603
1202	10	0.19708122178206366
1202	11	0.5162472438104118
1202	12	0.49968320254031084
1202	13	0.46521127866882495
1202	14	1.163134652863235
1202	15	0.8222522816742668
1202	16	0.6271570199452086
1202	17	-1.4528161652440588
1202	18	0.12723125385099365
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2210	18	0.5918350640863892
2210	19	0.7775927469165609
2210	20	0.9460812260575295
2210	21	0.951553564828366
2210	22	1.0536885896246773
2210	23	1.4432168574184772
2210	24	1.042234342982809
2210	25	0.9884024883189824
2211	1	0.024192886687774973
2211	2	-0.6144906555132224
2211	3	-0.17256171410453902
2211	4	0.2837618734274443
2211	5	0.21227920196483296
2211	6	0.20332051008588792
2211	7	0.1613257347392576
2211	8	0.1088973318882438
2211	9	0.5184378182143945
2211	10	0.6621193994989625
2211	11	0.2659299698088091
2211	12	0.10254345292086953
2211	13	0.14897313998930173
2211	14	-0.9915399015902864
2211	15	-0.02572656189678213
2211	16	0.09647962613239969
2211	17	0.20427729610950962
2211	18	0.33796782893866606
2211	19	0.451798478198441
2211	20	0.5224281073647177
2211	21	0.5549144311790855
2211	22	-0.04674467723562259
2211	23	0.45124656034060706
2211	24	-0.10809872052245595
2211	25	0.3401744686621697
2212	1	-0.14273509039340482
2212	2	-1.2061758114200778
2212	3	-0.3817890598083409
2212	4	-0.04871526465448994
2212	5	0.0432707738810052
2212	6	0.08291205698397164
2212	7	-0.039617428129396615
2212	8	0.04210469698576702
2212	9	-2.259526706898214
2212	10	-0.1680755440269479
2212	11	-0.13097060965134233
2212	12	-0.16943225132401807
2212	13	-0.13088420051337835
2212	14	0.5507966179430994
2212	15	0.1690175717863056
2212	16	0.10411108157341431
2212	17	0.10725134408215098
2212	18	0.005095668514449869
2212	19	-0.8079710356701355
2212	20	0.11074334648020567
2212	21	0.060355863943932775
2212	22	-1.8471427092719352
2212	23	-0.5017674131044885
2212	24	0.09512334504937314
2212	25	-0.6843432513817334
2213	1	0.006852133000176508
2213	2	0.2624552008204617
2213	3	-0.04768412801311328
2213	4	0.2343904064697213
2213	5	0.3148646360135743
2213	6	0.2138674643639149
2213	7	0.17981838859724775
2213	8	0.17821712677476143
2213	9	0.554627154050749
2213	10	0.5008636903002213
2213	11	-0.060338017463195115
2213	12	0.07562379726768853
2213	13	-0.07288379165605363
2213	14	-1.5852606146846284
2213	15	-0.5412188129020592
2213	16	-0.20969047069026203
2213	17	-2.200422540846283
2213	18	-0.8920471244078527
2213	19	-0.664264870645134
2213	20	0.0844404765265736
2213	21	-0.3673318714886804
2213	22	0.11825343634443186
2213	23	0.5361256515002353
2213	24	0.35425801289263603
2213	25	-1.3765378464131075
2214	1	0.185616306248356
2214	2	0.1268888528498131
2214	3	0.11214995356387902
2214	4	0.4678031966590339
2214	5	0.3900525558691754
2214	6	0.2996380182881282
2214	7	0.189045874550672
2214	8	0.20997443872606425
2214	9	0.45538198935704344
2214	10	0.5088130432861249
2214	11	-0.06849380578850628
2214	12	-0.09160663379130085
2214	13	0.059842405596533865
2214	14	0.7930645722218728
2214	15	0.3903827605231953
2214	16	0.3541841845511371
2214	17	0.289798851645493
2214	18	0.28060954495916945
2214	19	-0.8846476243477923
2214	20	0.1339891561800357
2214	21	0.32053123611306233
2214	22	0.5327472238856998
2214	23	1.0631789593008207
2214	24	0.7662920929640649
2214	25	0.7313755758215734
2215	1	-0.2258197123217549
2215	2	0.12468631428178867
2215	3	0.06444989111880162
2215	4	0.35878159337496385
2215	5	0.3458309970665283
2215	6	0.24479519140494688
2215	7	0.07078862323739088
2215	8	0.04315647719660376
2215	9	0.15913841342786128
2215	10	0.5022635366081545
2215	11	0.20649742623788114
2215	12	0.0406953036580191
2215	13	-0.061346991777469384
2215	14	0.627707448011023
2215	15	0.12835721555934754
2215	16	0.16733615971233462
2215	17	0.43151821218663855
2215	18	0.325540358346899
2215	19	-0.3945238727700726
2215	20	-1.6771635145196484
2215	21	-2.007839625720486
2215	22	-0.3631053254929597
2215	23	0.28463518624341333
2215	24	0.3998385074749251
2215	25	-0.22599208824572514
2216	1	-0.21256583280620234
2216	2	-0.25261153341934417
2216	3	-0.2367367030282317
2216	4	0.14290087425226972
2216	5	0.22304257130825533
2216	6	0.008617227540824501
2216	7	-0.03784985381503958
2216	8	-0.07878162266092659
2216	9	0.28293326498024385
2216	10	-1.7259439343944152
2216	11	-0.5876938955478792
2216	12	-0.37157200397272305
2216	13	-0.19714024209444148
2216	14	-0.5868010458496826
2216	15	-0.6926423171689109
2216	16	-0.25228994359672186
2216	17	-0.019114499008587416
2216	18	-0.10208355163383231
2216	19	-1.6048485076828243
2216	20	-1.2003828963993186
2216	21	-1.2033616440289592
2216	22	-0.929305484030543
2216	23	-4.648135688189129
2216	24	-2.2027117066154176
2216	25	-1.550765699345817
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	16.0
4	27.0
5	36.0
6	39.0
7	174.0
8	103.0
9	233.0
10	658.0
11	1482.0
12	2789.0
13	4549.0
14	6395.0
15	8431.0
16	9887.0
17	10573.0
18	11162.0
19	11339.0
20	11607.0
21	12030.0
22	12476.0
23	13559.0
24	14407.0
25	16155.0
26	18443.0
27	21500.0
28	25469.0
29	31793.0
30	40128.0
31	50124.0
32	67512.0
33	93037.0
34	130795.0
35	185430.0
36	273092.0
37	425766.0
38	758060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.22939641621577	21.646046176020718	14.351499829755255	22.77305757800826
2	15.562627409064364	21.79131910392172	40.48810481427202	22.1579486727419
3	17.56876671531843	26.73661881866588	31.074016653273105	24.620597812742588
4	12.914133405280822	18.270121380527712	38.99924217075096	29.816503043440502
5	13.345463876436	37.613045962619	35.685092041634775	13.356398119310226
6	29.670557310228652	39.001446540159776	18.342235005919267	12.9857611436923
7	25.05883229303352	33.07448152870959	24.776472548382646	17.090213629874242
8	22.835516203019004	37.212524415945886	22.1444418925835	17.807517488451605
9	23.958109708763114	17.817435949882235	23.079378108060094	35.14507623329455
10	14.661481302229962	30.375683267855102	34.4973074805626	20.465527949352335
11	30.687717578442843	26.26681453258707	24.665428377738657	18.380039511231434
12	20.446279345288886	27.980587505200877	31.976580185186492	19.596552964323745
13	25.553614633348626	25.01594538853193	27.374011595283783	22.056428382835666
14	20.486969662371052	24.37347830246112	28.61935332565991	26.520198709507913
15	22.195053032817707	31.39443182965315	24.887668112114564	21.522847025414578
16	21.388007084214525	31.629219475771652	26.373183656233994	20.60958978377983
17	19.787756131124677	30.591776563872614	28.9545032399455	20.665964065057207
18	19.859452116262535	30.426858107289085	29.70944063388039	20.00424914256799
19	20.58107303433433	29.47192768806221	29.876936950516942	20.07006232708652
20	21.826507765809282	28.944431712003947	29.20587768630009	20.023182835886676
21	22.17552974173892	29.352313349196535	28.15349431882037	20.318662590244173
22	21.298985666042864	28.975951990367044	29.495680243715178	20.229382099874915
23	21.52843462983809	29.18682767855333	28.850044384344624	20.43469330726396
24	21.22170410070167	28.610724233983287	29.119168929163287	21.048402736151758
25	21.70742144246761	28.90374579254547	28.65537914514229	20.733453619844628
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2073.0
1	2073.0
2	2244.0
3	2415.0
4	2415.0
5	2415.0
6	3463.0
7	4511.0
8	4511.0
9	4511.0
10	7675.0
11	10839.0
12	10839.0
13	10839.0
14	18369.0
15	25899.0
16	25899.0
17	25899.0
18	40295.5
19	54692.0
20	54692.0
21	54692.0
22	76330.5
23	97969.0
24	97969.0
25	97969.0
26	124694.5
27	151420.0
28	151420.0
29	151420.0
30	178776.5
31	206133.0
32	206133.0
33	206133.0
34	227870.5
35	249608.0
36	249608.0
37	249608.0
38	262335.0
39	275062.0
40	275062.0
41	275062.0
42	271584.5
43	268107.0
44	268107.0
45	268107.0
46	256141.0
47	244175.0
48	244175.0
49	244175.0
50	231384.5
51	218594.0
52	218594.0
53	218594.0
54	197136.0
55	175678.0
56	175678.0
57	175678.0
58	150927.0
59	126176.0
60	126176.0
61	126176.0
62	103354.0
63	80532.0
64	80532.0
65	80532.0
66	61847.0
67	43162.0
68	43162.0
69	43162.0
70	31544.5
71	19927.0
72	19927.0
73	19927.0
74	13940.5
75	7954.0
76	7954.0
77	7954.0
78	5474.0
79	2994.0
80	2994.0
81	2994.0
82	2000.5
83	1007.0
84	1007.0
85	1007.0
86	635.0
87	263.0
88	263.0
89	263.0
90	166.5
91	70.0
92	70.0
93	70.0
94	41.0
95	12.0
96	12.0
97	12.0
98	8.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08601862444233314
2	0.022077526047955383
3	0.03406372781451
4	0.042083906937719344
5	0.0516464282000074
6	0.01824370415938828
7	0.02375206894181228
8	0.034328129324066356
9	0.034328129324066356
10	0.025691013345225527
11	0.024413072715703157
12	0.018860641015019768
13	0.026219816364338233
14	0.03697214441962987
15	0.034151861651028785
16	0.02511814340785343
17	0.024897808816556472
18	0.025691013345225527
19	0.01934537711587308
20	0.015687822900343547
21	0.01291160705000185
22	0.01753863346723801
23	0.02018264856280153
24	0.017450499630719225
25	0.10866902042766062
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2269276.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.1691703313536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.06819042857073	77.56202222379086
2	6.924342666840996	11.794810400496802
3	1.047296179860463	2.6759204018973515
4	0.368508383410169	1.2554221310076978
5	0.16357045196792733	0.6965579842416448
6	0.10232182101111127	0.5228798761385768
7	0.0694369229670632	0.41397195836268197
8	0.04417477032117722	0.30098628302662134
9	0.03047744844368693	0.23361650979888782
>10	0.16715041236189318	2.4390106327382797
>50	0.009545412694087558	0.5720913452796083
>100	0.004362932182369052	0.7626381805546997
>500	1.555423420697164E-4	0.08103000477914261
>1k	4.666270262087975E-4	0.6890420678871009
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	3783	0.16670515177527986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	4.406691825939198E-5	0.0	0.0	0.0
4	0.0	4.406691825939198E-5	0.0	0.0	0.0
5	0.0	4.406691825939198E-5	0.0	0.0	0.0
6	0.0	4.406691825939198E-5	0.0	0.0	0.0
7	0.0	4.406691825939198E-5	0.0	0.0	0.0
8	0.0	4.406691825939198E-5	0.0	0.0	0.0
9	0.0	4.406691825939198E-5	0.0	4.406691825939198E-5	0.0
10	0.0	4.406691825939198E-5	0.0	8.813383651878396E-5	0.0
11	0.0	4.406691825939198E-5	0.0	8.813383651878396E-5	0.0
12	0.0	4.406691825939198E-5	0.0	8.813383651878396E-5	0.0
13	0.0	4.406691825939198E-5	0.0	8.813383651878396E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGGTAT	900	0.0	11.938487	1
GACCGTG	65	8.032326E-4	11.6911955	7
GTCTTAC	160	5.366019E-10	10.697096	1
TGGTATC	925	0.0	10.682421	2
GGTATCA	1885	0.0	10.492171	1
CTAGTCC	120	1.5210771E-6	10.293411	3
CCCGTGT	75	0.002650465	10.132369	8
GTACTAG	145	1.5967817E-7	9.8364105	1
GGTCTAG	110	6.771588E-5	9.508531	1
GTCTCGC	160	6.546361E-8	9.50853	1
CCTATAC	160	6.620394E-8	9.50161	3
GTCTAGT	205	4.1654857E-10	9.276615	1
GTTCTAC	160	6.6555003E-7	8.914247	1
GCCCTAG	120	1.7470414E-4	8.716152	1
TAAGACA	330	0.0	8.637827	4
GTTTTAG	335	0.0	8.515101	1
GTGTAAG	280	3.6379788E-12	8.4897585	1
GTATTAA	360	0.0	8.452026	1
TACCCTG	225	2.4392648E-9	8.445875	5
TCGCCCA	180	4.0456507E-7	8.442896	12
>>END_MODULE