FastQCFastQC Report
Wed 25 May 2016
SRR1295349_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295349_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2269276
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87190.3842194603036387No Hit
GGTATCAACGCAGAGTACTTTTTTT79550.3505523347534632No Hit
TATCAACGCAGAGTACTTTTTTTTT52540.23152758853484545No Hit
GTGGTATCAACGCAGAGTACATGGG38580.17001017064473428No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35070.1545426823356877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA2100.015.8431123
CAGCGTC2500.013.3082132
GCAGCGT2500.012.9459671
GGTATCA25800.011.9173151
TGGTATC9600.011.6842952
GTGGTAT10450.010.9310161
GCGTCAG3200.010.3970414
CCTATAC1409.606447E-810.1848563
CTGTCGC1701.5697879E-910.0554989
TGTCGCC1701.5697879E-910.05549810
CGTCAGA3450.09.643425
TGTGCCG800.0045291339.496860510
GTCTAGA2550.09.3324431
TAGTACC1752.6437192E-89.234274
CCGTGTG850.0074571538.9382219
GTCTTAG2451.6370905E-118.9362921
GACTGTC1502.7143742E-68.8637367
GTGTTAG3450.08.5533881
CTTATAC2901.8189894E-128.5225013
GTAACAC1451.6763426E-58.5225013
Produced by FastQC (version 0.11.5)