FastQCFastQC Report
Wed 25 May 2016
SRR1295348_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295348_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3836331
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG55280.1440960125703439No Hit
GTATCAACGCAGAGTACTTTTTTTT52270.1362499742592597No Hit
GGTATCAACGCAGAGTACTTTTTTT42960.111981995297069No Hit
GTACATAAGCAGTGGTATCAACGCA42930.11190379557968277No Hit
ATTCCATTCCATTCCATTCCATTCC40870.10653408165249559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG1403.6379788E-1212.9021381
AGGACCG701.0895608E-412.2168595
CGTCAGA1604.5474735E-1111.2831872
CGCAGAT1303.853038E-710.2294568
GTATTAG6400.09.8039271
TAACCCG1054.485814E-49.0495265
TAATACG950.00182123359.0018974
TCTGTCG2401.0186341E-108.7072168
GTACTAG2950.08.7011741
GTCTTAC3500.08.6919661
GGTATCA43650.08.6465411
GTTCTAC2701.4551915E-118.4505221
TAGGACT4050.08.4462244
TATTCCG1606.437025E-68.3142525
GTGTTAG6450.08.2541
TATACTG11700.08.2025835
TTAGGGT4350.08.0821634
GTCTAGA3750.07.85898641
TCTACAC4600.07.8493773
GTCCTAT3157.2759576E-127.8469141
Produced by FastQC (version 0.11.5)