##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295348_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3836331 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.359820620274945 31.0 30.0 33.0 27.0 34.0 2 30.195051469750656 31.0 30.0 34.0 26.0 34.0 3 30.556924050609815 31.0 30.0 34.0 27.0 34.0 4 33.90395693176632 35.0 35.0 37.0 28.0 37.0 5 34.222372365679604 35.0 35.0 37.0 30.0 37.0 6 34.560414625328214 37.0 35.0 37.0 31.0 37.0 7 34.52576042056851 37.0 35.0 37.0 31.0 37.0 8 34.50464910353147 37.0 35.0 37.0 30.0 37.0 9 35.61226416594397 39.0 35.0 39.0 29.0 39.0 10 35.52465884721626 39.0 35.0 39.0 28.0 39.0 11 35.924165302733265 39.0 35.0 39.0 30.0 39.0 12 35.91792392262295 39.0 35.0 39.0 30.0 39.0 13 35.84650386006838 39.0 35.0 39.0 30.0 39.0 14 36.13298800338136 39.0 36.0 41.0 27.0 41.0 15 36.74197221251242 39.0 36.0 41.0 29.0 41.0 16 36.84707523933675 40.0 36.0 41.0 30.0 41.0 17 36.65634821395756 39.0 36.0 41.0 29.0 41.0 18 36.63253040470178 39.0 36.0 41.0 29.0 41.0 19 36.692863832656776 40.0 36.0 41.0 28.0 41.0 20 36.67770612077008 40.0 36.0 41.0 28.0 41.0 21 36.67849359192416 40.0 36.0 41.0 28.0 41.0 22 36.440377277143185 39.0 36.0 41.0 27.0 41.0 23 36.13691675718284 39.0 35.0 41.0 27.0 41.0 24 36.45384900312304 39.0 36.0 41.0 27.0 41.0 25 35.31602330455844 39.0 34.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29949967506270525 1101 2 0.5252080831553556 1101 3 0.3579860371522905 1101 4 0.2917654041622342 1101 5 0.2645639293424722 1101 6 0.35747142945700006 1101 7 0.39668302564169267 1101 8 0.43785620690914584 1101 9 0.4380687236797485 1101 10 0.6537559669590678 1101 11 0.5494773987693051 1101 12 0.6244863828793044 1101 13 0.5440034875105653 1101 14 1.1347098378945333 1101 15 0.8175114023112187 1101 16 0.6233481794523712 1101 17 0.9569524235922273 1101 18 0.8410681038796142 1101 19 0.9357371817686726 1101 20 1.0267474169622943 1101 21 0.9883869376848438 1101 22 0.7746474563366306 1101 23 -1.3598006694526532 1101 24 0.1772411441595878 1101 25 0.6910628360231073 1102 1 0.18507179021854725 1102 2 0.2823539313590189 1102 3 0.2123955541639475 1102 4 0.4273598842954698 1102 5 0.11926419106029584 1102 6 0.2595334803730154 1102 7 0.23435611629122377 1102 8 0.29627476508188266 1102 9 0.5455307270106999 1102 10 0.936370956252432 1102 11 0.6062082100945503 1102 12 0.5464382268003121 1102 13 0.4796465995733783 1102 14 0.9938828095814216 1102 15 0.8039341479690805 1102 16 0.5385282888980072 1102 17 0.8694868037968462 1102 18 0.8372165692614786 1102 19 0.9409753449854179 1102 20 0.9563847236480001 1102 21 0.9946299069924791 1102 22 0.8569945417518099 1102 23 0.896766068591603 1102 24 0.9716463785159988 1102 25 0.78503828213303 1103 1 -0.22942070226428513 1103 2 -1.5266984198916447 1103 3 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22 0.05083894404776146 2211 23 0.5025287497793371 2211 24 0.007549180409100131 2211 25 0.3937634390062854 2212 1 -0.07454286076704264 2212 2 -1.133822326747314 2212 3 -0.36690203573466107 2212 4 0.09185048965226628 2212 5 0.20357351044995653 2212 6 0.02659130221601913 2212 7 0.064515606939338 2212 8 0.05981390619860605 2212 9 -2.041856646719438 2212 10 -0.020298200355902907 2212 11 0.05487195990894378 2212 12 -0.10504559851861472 2212 13 -0.02323804560494125 2212 14 0.6096268373585971 2212 15 0.2485109839373436 2212 16 0.2816216438300856 2212 17 0.24768409380297385 2212 18 0.03870432606726126 2212 19 -0.5681077778634389 2212 20 0.16645306155196238 2212 21 0.2735421889684915 2212 22 -1.715008529694522 2212 23 -0.39039036915702496 2212 24 0.23997321545602546 2212 25 -0.4832612343051963 2213 1 0.17098679041117748 2213 2 0.47015069084069694 2213 3 0.13347134820530826 2213 4 0.3463764793924682 2213 5 0.3012799202526537 2213 6 0.16296241994002258 2213 7 0.1514485184911436 2213 8 0.20065335694246755 2213 9 0.5497271924206686 2213 10 0.5573687815574573 2213 11 -0.06834947771504574 2213 12 -0.027327952384759158 2213 13 0.02964724167530619 2213 14 -1.7852837377201425 2213 15 -0.4499158842402906 2213 16 -0.08774980950998668 2213 17 -2.2091164335164564 2213 18 -0.8640661418531295 2213 19 -0.5266896639606529 2213 20 0.18699876078651556 2213 21 -0.2774197361579951 2213 22 0.25322252767122677 2213 23 0.7036469700983261 2213 24 0.5444664726404582 2213 25 -0.8000476053116614 2214 1 0.1358391099114904 2214 2 0.06189049993783158 2214 3 0.03549370706162591 2214 4 0.32018531019095775 2214 5 0.32233193940137284 2214 6 0.14868852958273493 2214 7 0.09550375226468333 2214 8 0.08167649676422428 2214 9 0.42150685484247674 2214 10 0.4436474102785084 2214 11 -0.19119262968678186 2214 12 -0.28685812112440345 2214 13 -0.2639909536250329 2214 14 0.6012671802279357 2214 15 0.2880426444527018 2214 16 0.18702523590131648 2214 17 0.08080484897770646 2214 18 0.08643201096716524 2214 19 -1.0049654851627068 2214 20 -0.017177723663735378 2214 21 0.21507877806602238 2214 22 0.45387038003459423 2214 23 0.9639336962412131 2214 24 0.5835274521471447 2214 25 0.6037837413238805 2215 1 -0.23479962545244604 2215 2 0.22292450376793838 2215 3 0.12976650753527963 2215 4 0.2934563602345861 2215 5 0.29577947930215487 2215 6 0.08964263701472674 2215 7 0.05659711244364729 2215 8 0.06846911363901853 2215 9 0.15318382432327127 2215 10 0.4371392089479116 2215 11 0.09267509458391743 2215 12 -0.02785449172363741 2215 13 -0.14721223361716795 2215 14 0.5820100238752133 2215 15 0.13083279338085418 2215 16 0.200083412092809 2215 17 0.35072941136358793 2215 18 0.18375866824045062 2215 19 -0.5185190107295625 2215 20 -1.9014331521714922 2215 21 -2.009170994473358 2215 22 -0.4218361197590923 2215 23 0.2613221271700752 2215 24 0.4123975094717238 2215 25 -0.3371152709240093 2216 1 0.13973756548769245 2216 2 -0.07228474838586507 2216 3 -5.03107752983567E-4 2216 4 0.30657459515045815 2216 5 0.3593534582723237 2216 6 0.10471185444419717 2216 7 0.09793878601035289 2216 8 0.02171857250054643 2216 9 0.3029242280320261 2216 10 -1.6502573202913169 2216 11 -0.5379415208415494 2216 12 -0.23650575741767454 2216 13 -0.034781458725994696 2216 14 -0.4959972793609708 2216 15 -0.5324675787435709 2216 16 -0.07384741706579945 2216 17 0.18655470213805359 2216 18 0.003766260561640422 2216 19 -1.2931812770126925 2216 20 -0.9908681232322749 2216 21 -0.8532372558635402 2216 22 -0.7048687751021845 2216 23 -4.723740761618551 2216 24 -2.2510018409914636 2216 25 -1.3390491455437115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 16.0 5 32.0 6 49.0 7 198.0 8 226.0 9 526.0 10 1229.0 11 2639.0 12 4604.0 13 7658.0 14 10696.0 15 13934.0 16 16372.0 17 18405.0 18 19544.0 19 20350.0 20 20163.0 21 21498.0 22 22392.0 23 23955.0 24 26459.0 25 29148.0 26 33397.0 27 38526.0 28 45585.0 29 57161.0 30 71298.0 31 88888.0 32 119451.0 33 163518.0 34 227693.0 35 321844.0 36 471077.0 37 731995.0 38 1205796.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.29179158444616 22.194763210153013 14.146345026646229 22.367100178754605 2 15.337530796605531 21.79946032615072 41.17382972898987 21.68917914825388 3 17.53507630612144 27.464884582591303 30.964327898602683 24.035711212684575 4 12.088694652218866 18.56574588228545 39.70993140727857 29.63562805821711 5 13.141820863621167 38.06511138846888 35.64830003040736 13.144767717502589 6 29.217464798572834 39.73601178147338 18.527978001242488 12.518545418711302 7 24.51756159482917 33.56638251820868 24.890638430668325 17.025417456293827 8 22.403817871946295 37.65441917321857 22.35805535100766 17.583707603827467 9 23.59110968332758 17.644935963560922 23.33838504470921 35.42556930840229 10 14.292224787977561 30.559440978777292 34.62995374079568 20.518380492449477 11 30.588677584595874 26.577934158713205 24.644492925315355 18.18889533137556 12 20.02041268558468 28.23204765566954 32.601040186660065 19.14649947208572 13 25.29839469131206 25.22432397763643 27.90140278094988 21.575878550101628 14 20.229054212626142 24.329943414430623 29.06657279198936 26.374429580953873 15 22.04904063181873 31.672187709907423 25.002164262857285 21.276607395416562 16 21.17849560597682 32.047233891558214 26.656430849179163 20.1178396532858 17 19.209857926266825 31.13624050803861 29.479243740376376 20.17465782531819 18 19.282398734522104 30.833534747062163 30.38299374587558 19.50107277254015 19 20.090651020423593 29.598997238913615 30.348956374000895 19.961395366661897 20 21.45125764069874 29.358714430847943 29.5325210193351 19.65750690911821 21 21.983058071529403 29.815161188150817 28.400292488748175 19.801488251571605 22 20.51377511089281 29.456947205793178 30.144229741433477 19.88504794188054 23 20.977872049100345 29.648096311087023 29.327506030783656 20.046525609028983 24 20.513145394497403 28.769674292882996 29.69005004541525 21.02713026720435 25 21.312219792378876 29.261165246165167 28.938955840061798 20.48765912139416 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2245.0 1 2245.0 2 2790.0 3 3335.0 4 3335.0 5 3335.0 6 5543.5 7 7752.0 8 7752.0 9 7752.0 10 13186.0 11 18620.0 12 18620.0 13 18620.0 14 32566.0 15 46512.0 16 46512.0 17 46512.0 18 72243.0 19 97974.0 20 97974.0 21 97974.0 22 136207.0 23 174440.0 24 174440.0 25 174440.0 26 222008.0 27 269576.0 28 269576.0 29 269576.0 30 317200.0 31 364824.0 32 364824.0 33 364824.0 34 401271.5 35 437719.0 36 437719.0 37 437719.0 38 460355.0 39 482991.0 40 482991.0 41 482991.0 42 477364.5 43 471738.0 44 471738.0 45 471738.0 46 441269.5 47 410801.0 48 410801.0 49 410801.0 50 381736.5 51 352672.0 52 352672.0 53 352672.0 54 313839.5 55 275007.0 56 275007.0 57 275007.0 58 235035.5 59 195064.0 60 195064.0 61 195064.0 62 157701.0 63 120338.0 64 120338.0 65 120338.0 66 90998.0 67 61658.0 68 61658.0 69 61658.0 70 44232.0 71 26806.0 72 26806.0 73 26806.0 74 18614.0 75 10422.0 76 10422.0 77 10422.0 78 7122.5 79 3823.0 80 3823.0 81 3823.0 82 2580.0 83 1337.0 84 1337.0 85 1337.0 86 910.5 87 484.0 88 484.0 89 484.0 90 322.0 91 160.0 92 160.0 93 160.0 94 94.0 95 28.0 96 28.0 97 28.0 98 16.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08192723724829791 2 0.01775133584667225 3 0.029220627729984718 4 0.03586760370781353 5 0.045173370076773876 6 0.01245982163687127 7 0.0172039378249687 8 0.03427754278762703 9 0.03459034165717192 10 0.020071260795796818 11 0.017803468991596398 12 0.012538021354257491 13 0.021035723976893548 14 0.03730126519322759 15 0.03406901020793044 16 0.020331926520417556 17 0.018689732455306907 18 0.02455471125927351 19 0.013606750825202518 20 0.011938490187629795 21 0.007455039724153103 22 0.016812939238037592 23 0.01548354404247183 24 0.01603094206417538 25 0.11341565678248307 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3836331.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.59276381591546 #Duplication Level Percentage of deduplicated Percentage of total 1 85.59422394012013 67.27086626132439 2 11.094429806327971 17.43883802881974 3 2.0519160469036883 4.837972597331655 4 0.5559713768874345 1.7478130844849382 5 0.20920908887251127 0.8221160254950073 6 0.11345751897656074 0.535016399523875 7 0.0745829331531059 0.41031751950001843 8 0.05023083632823935 0.3158224204656978 9 0.03815823437383676 0.26990649915977694 >10 0.19501573033271208 2.76712251076151 >50 0.012241957202667084 0.6691209496079913 >100 0.009199794830581061 1.3396035556778674 >500 8.309364498459745E-4 0.44271965469586994 >1k 4.6532433549754146E-4 0.8518198818647704 >5k 6.64749050710746E-5 0.28094461128692816 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGTATCAACGCAGAGTACATGGG 5528 0.1440960125703439 No Hit GTATCAACGCAGAGTACTTTTTTTT 5227 0.1362499742592597 No Hit GGTATCAACGCAGAGTACTTTTTTT 4296 0.111981995297069 No Hit GTACATAAGCAGTGGTATCAACGCA 4293 0.11190379557968277 No Hit ATTCCATTCCATTCCATTCCATTCC 4087 0.10653408165249559 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.6066572462073788E-5 0.0 4 0.0 0.0 0.0 5.2133144924147577E-5 0.0 5 0.0 0.0 0.0 5.2133144924147577E-5 0.0 6 0.0 0.0 0.0 5.2133144924147577E-5 0.0 7 0.0 0.0 0.0 5.2133144924147577E-5 2.6066572462073788E-5 8 0.0 0.0 0.0 5.2133144924147577E-5 2.6066572462073788E-5 9 0.0 0.0 0.0 1.0426628984829515E-4 2.6066572462073788E-5 10 0.0 0.0 0.0 1.0426628984829515E-4 2.6066572462073788E-5 11 0.0 0.0 0.0 1.0426628984829515E-4 2.6066572462073788E-5 12 0.0 0.0 0.0 1.0426628984829515E-4 2.6066572462073788E-5 13 0.0 0.0 0.0 1.0426628984829515E-4 2.6066572462073788E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCAG 140 3.6379788E-12 12.902138 1 AGGACCG 70 1.0895608E-4 12.216859 5 CGTCAGA 160 4.5474735E-11 11.283187 2 CGCAGAT 130 3.853038E-7 10.229456 8 GTATTAG 640 0.0 9.803927 1 TAACCCG 105 4.485814E-4 9.049526 5 TAATACG 95 0.0018212335 9.001897 4 TCTGTCG 240 1.0186341E-10 8.707216 8 GTACTAG 295 0.0 8.701174 1 GTCTTAC 350 0.0 8.691966 1 GGTATCA 4365 0.0 8.646541 1 GTTCTAC 270 1.4551915E-11 8.450522 1 TAGGACT 405 0.0 8.446224 4 TATTCCG 160 6.437025E-6 8.314252 5 GTGTTAG 645 0.0 8.254 1 TATACTG 1170 0.0 8.202583 5 TTAGGGT 435 0.0 8.082163 4 GTCTAGA 375 0.0 7.8589864 1 TCTACAC 460 0.0 7.849377 3 GTCCTAT 315 7.2759576E-12 7.846914 1 >>END_MODULE