FastQCFastQC Report
Wed 25 May 2016
SRR1295348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295348_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3836331
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT144830.37752216896821467No Hit
GGTATCAACGCAGAGTACTTTTTTT122670.31975864439225915No Hit
TATCAACGCAGAGTACTTTTTTTTT85190.2220611308044066No Hit
GTGGTATCAACGCAGAGTACATGGG60360.15733783138107738No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57190.1490747279106No Hit
GTACATAAGCAGTGGTATCAACGCA48190.12561481269473357No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCT307.690292E-419.0129284
GGCGTTC456.784844E-414.77222258
GACAGCG550.003078514712.0863647
GCAGCGT2400.011.89829351
CAGCGTC2600.011.3347792
TAGACGG600.005849203611.0907315
AGCGTCA2750.010.7165183
GTCTAAG3300.010.3839661
TAGAACG1054.0778847E-59.9591544
GTACGGA900.00109450239.5186361
AATACCG800.0044932019.5063415
GGTATCA51800.09.3716291
GTCCTAC2252.5465852E-119.307111
ATACCGT1356.881799E-69.1451856
TCTGTCG3550.09.0951718
AACCGAA950.00183098258.9966167
GTGCTAG4350.08.9715881
TAGACAG5650.08.9174525
ACTGATC3350.08.7877418
CGAATAG1201.771927E-48.705173510
Produced by FastQC (version 0.11.5)