FastQCFastQC Report
Wed 25 May 2016
SRR1295347_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295347_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3837743
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG57690.1503227287496844No Hit
GTATCAACGCAGAGTACTTTTTTTT53870.14036896165272142No Hit
GGTATCAACGCAGAGTACTTTTTTT44760.11663105111519975No Hit
GTACATAAGCAGTGGTATCAACGCA41340.10771956329540565No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC600.005884330711.0818136
AGGACCG700.001490082810.859055
AGTACCG750.002645229510.1351145
TAGACAG6100.09.3459045
CCGTACC1151.10929584E-49.0885524
TAGTACC2751.8189894E-128.637884
AACCCGT1107.158122E-48.6351796
CTAGAAC4000.08.5515023
TATACTG11500.08.510195
GGTATCA43900.08.4685041
GTCTAAG3300.08.3556171
ATTAGAC4250.08.2720413
GTCTTAC2909.094947E-128.19665051
CTAGTAC2909.094947E-128.1910933
CATAAGC13400.08.1544184
TCAGGAC4550.08.1442883
TAGTACT4550.08.1442884
GTCTAGC1659.641222E-68.0674921
GTCTTAA4600.08.0612281
TGTCGCC3300.08.05939610
Produced by FastQC (version 0.11.5)