##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295347_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3837743
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.631331488325298	31.0	30.0	34.0	27.0	34.0
2	30.348959271113255	31.0	30.0	34.0	26.0	34.0
3	30.788511372439476	31.0	31.0	34.0	27.0	34.0
4	34.1031569857596	35.0	35.0	37.0	29.0	37.0
5	34.385513829352305	37.0	35.0	37.0	30.0	37.0
6	34.522617069459834	37.0	35.0	37.0	30.0	37.0
7	34.39555905645584	37.0	35.0	37.0	30.0	37.0
8	34.460135032491756	37.0	35.0	37.0	30.0	37.0
9	35.44834372702914	39.0	35.0	39.0	28.0	39.0
10	35.36311576882558	39.0	35.0	39.0	28.0	39.0
11	35.79631752308583	39.0	35.0	39.0	30.0	39.0
12	35.79047059691074	39.0	35.0	39.0	30.0	39.0
13	35.71290391253401	39.0	35.0	39.0	30.0	39.0
14	35.983710998886586	39.0	36.0	41.0	27.0	41.0
15	36.609018634129484	39.0	36.0	41.0	29.0	41.0
16	36.71234889881892	39.0	36.0	41.0	29.0	41.0
17	36.52300271279239	39.0	36.0	41.0	28.0	41.0
18	36.48924120244634	39.0	36.0	41.0	27.0	41.0
19	36.55755896108729	40.0	36.0	41.0	27.0	41.0
20	36.548160468275235	40.0	36.0	41.0	27.0	41.0
21	36.54628723184434	40.0	36.0	41.0	27.0	41.0
22	36.29810203549326	39.0	36.0	41.0	27.0	41.0
23	36.00197668264915	39.0	35.0	41.0	27.0	41.0
24	36.324625958538654	39.0	35.0	41.0	27.0	41.0
25	35.158779782804636	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4783249978922832
1101	2	0.7013009595419994
1101	3	0.4808175274010935
1101	4	0.5370875403549746
1101	5	0.3425192273537476
1101	6	0.28932134304608326
1101	7	0.35256836797528024
1101	8	0.41243550942053275
1101	9	0.3831735367155602
1101	10	0.6871283206074921
1101	11	0.5777045032050552
1101	12	0.5728004379747844
1101	13	0.5436413130823041
1101	14	1.1561992732709783
1101	15	0.8220188983442469
1101	16	0.647692507626104
1101	17	0.990709343484248
1101	18	0.9521337437198127
1101	19	0.9260552097829731
1101	20	1.0662215221365088
1101	21	0.9725648121853538
1101	22	0.8664147525352135
1101	23	-1.2729112552908362
1101	24	0.23573916442777687
1101	25	0.7409117879671498
1102	1	0.46933124542803384
1102	2	0.512347829119868
1102	3	0.4527197235535674
1102	4	0.5444022442655623
1102	5	0.3604517569527417
1102	6	0.35541953744233723
1102	7	0.24381684278301918
1102	8	0.32063050268543236
1102	9	0.48803474888426024
1102	10	0.9304363802146796
1102	11	0.6531323965927172
1102	12	0.6323248516230962
1102	13	0.5617525746914893
1102	14	1.2233169988346333
1102	15	0.8664048848591221
1102	16	0.7288893280122437
1102	17	0.9302576875701121
1102	18	0.9529530875730856
1102	19	0.8920438888452082
1102	20	1.013333265090779
1102	21	0.9413956038425368
1102	22	0.9251114267765388
1102	23	0.9709900279229089
1102	24	0.9336749776211093
1102	25	0.9115396634443016
1103	1	-0.21247736219378055
1103	2	-1.4540471179119479
1103	3	-0.30946659788107667
1103	4	-0.018474722544937094
1103	5	-0.16679431547061796
1103	6	-0.01318590578036094
1103	7	0.027037554181525536
1103	8	0.18962541203206484
1103	9	0.7001814404326083
1103	10	0.709300103600178
1103	11	0.49926923165224224
1103	12	0.35491255968328517
1103	13	0.14648550263316196
1103	14	-0.9504113693049376
1103	15	-0.15570914356875676
1103	16	-0.05943793600925318
1103	17	0.4757850817290077
1103	18	0.5546304855819741
1103	19	0.5384102250963849
1103	20	0.5681904358795578
1103	21	0.7261208975123452
1103	22	0.16998490153179802
1103	23	1.0438805051608213
1103	24	0.9028141234604021
1103	25	0.75132954095168
1104	1	0.060909975395837535
1104	2	0.10637659412167011
1104	3	-0.26961045339839984
1104	4	-0.2248057842055715
1104	5	-0.1016177381473895
1104	6	-0.10035937205272205
1104	7	0.07162660642371321
1104	8	0.0710003819676217
1104	9	-2.0357061081805483
1104	10	-0.4465502310541112
1104	11	-0.32808641132914573
1104	12	0.10337393321655242
1104	13	0.20866968069971392
1104	14	-1.7465839524565823
1104	15	-0.5725896531200405
1104	16	-0.21036508289100198
1104	17	0.13459176222514913
1104	18	0.25735015698807473
1104	19	0.40473995124284556
1104	20	0.5360957885233049
1104	21	0.578427280593445
1104	22	0.6747046181840801
1104	23	1.1298042336320648
1104	24	0.8364658955899742
1104	25	0.6888852315167426
1105	1	-0.1431348295228858
1105	2	-0.028293807062294007
1105	3	-1.3112340455965423
1105	4	-0.4767043674455351
1105	5	-0.36636924806337134
1105	6	-0.15429037354408592
1105	7	-0.16840467677189963
1105	8	-0.17970054678506386
1105	9	0.2722242687373324
1105	10	0.3835375111208208
1105	11	-0.13480727029639183
1105	12	-0.14233333567384676
1105	13	-0.1942852202686396
1105	14	0.4926613131885702
1105	15	0.18353106100189365
1105	16	-0.3239693050319872
1105	17	0.25115972992502833
1105	18	0.20383200402128665
1105	19	0.12553712751644497
1105	20	-0.47482465569726884
1105	21	0.08757088090114706
1105	22	0.12717418234264954
1105	23	0.33175591975329866
1105	24	0.19024850787209857
1105	25	0.4592905651683168
1106	1	-1.2683287671830819
1106	2	-0.8106099485121661
1106	3	-0.8818604682536666
1106	4	-1.1198637242032277
1106	5	-0.7724542473861362
1106	6	-0.6032232332647354
1106	7	-0.43390919599422517
1106	8	-0.5588310216303967
1106	9	-0.22669131947037613
1106	10	-1.6976434727385339
1106	11	-0.9426462667921456
1106	12	-0.6703038282436609
1106	13	-0.6249386283495824
1106	14	-0.024953581135129355
1106	15	-0.701263683762356
1106	16	-1.27395888896406
1106	17	-0.5152775838967543
1106	18	-0.4407700972002999
1106	19	-0.38741229868059435
1106	20	-0.2620766068134017
1106	21	-0.19452204327176048
1106	22	-0.15087192501051305
1106	23	-0.3356342787766238
1106	24	-0.7148150965924245
1106	25	-1.2622467955119276
1107	1	0.020788562390627874
1107	2	0.2025937864848082
1107	3	-0.009500227690661944
1107	4	0.16969549886044888
1107	5	-0.1801570765151581
1107	6	0.036580982360788994
1107	7	-0.002153492311727234
1107	8	-0.07728456297778052
1107	9	0.42369377504902417
1107	10	0.07867242718057099
1107	11	0.061924648222365875
1107	12	-0.04727374971385956
1107	13	0.1381822730309139
1107	14	-1.0349727914079452
1107	15	-0.4148925485274262
1107	16	-0.1231179054910072
1107	17	0.1527239108256211
1107	18	0.5419544985577787
1107	19	0.3539182995588419
1107	20	0.4835617163498398
1107	21	0.4370774730916125
1107	22	-0.14248064240626945
1107	23	0.6131551718574002
1107	24	0.5019120417833847
1107	25	0.08453126202665828
1108	1	-0.10349565349847012
1108	2	0.2008723641848107
1108	3	-0.15058059231511933
1108	4	-0.3585838748172421
1108	5	-0.33057933730836453
1108	6	-0.25825259892689445
1108	7	-0.17945478188100594
1108	8	-0.30338639351431596
1108	9	0.21887036513845004
1108	10	0.18495740807890115
1108	11	-0.03501182402379044
1108	12	-0.09145042898915534
1108	13	-0.2840781344462968
1108	14	0.006983057756571043
1108	15	-0.10527552069931545
1108	16	-0.15663559210706524
1108	17	0.2077025501622316
1108	18	0.17057783984730435
1108	19	0.14054150457610604
1108	20	0.2913971620547784
1108	21	0.1721997642223414
1108	22	0.18307398584033052
1108	23	-0.053252368114996784
1108	24	0.08367296099793009
1108	25	-0.7122091252890854
1109	1	-0.7005117151742795
1109	2	-0.640711097820077
1109	3	-0.7017162629618561
1109	4	-0.5156446014994387
1109	5	-0.7515753892467529
1109	6	-1.0363337652634783
1109	7	-0.7048185584129243
1109	8	-1.0071900217927663
1109	9	-0.6529774025328763
1109	10	-0.920980948340258
1109	11	-0.9987909884263502
1109	12	-0.8829573944718447
1109	13	-0.9301716185938389
1109	14	-0.44701452985184176
1109	15	-1.0654936315393044
1109	16	-1.303716036470128
1109	17	-1.1734087140044736
1109	18	-1.7371950368402551
1109	19	-1.8195125865943211
1109	20	-1.534375372244483
1109	21	-1.4780565744590746
1109	22	-1.56315427920795
1109	23	-1.0604118377288074
1109	24	-1.9498589067766119
1109	25	-1.0362243256960681
1110	1	0.022298157555027842
1110	2	0.08092599569247128
1110	3	-0.021074810922858234
1110	4	0.0998127856220421
1110	5	0.13721678691860717
1110	6	0.13645739118739897
1110	7	0.030766572687078053
1110	8	0.02445081497546653
1110	9	0.309416000731936
1110	10	0.15736646881331495
1110	11	0.16150436305245108
1110	12	0.17687209377777435
1110	13	0.09619854206497536
1110	14	-2.586472747373719
1110	15	-0.7875823897249887
1110	16	-0.30149299436495625
1110	17	-0.22011744568618496
1110	18	-2.7993381367722776
1110	19	-0.5531746738681136
1110	20	-2.0320613303028097
1110	21	-0.3394518837724618
1110	22	-1.8425324900177884
1110	23	-0.6232274329064609
1110	24	-0.005383113163375697
1110	25	0.027825037950464093
1111	1	-0.1899082092787694
1111	2	-1.3440596587172422
1111	3	-0.4289953574196268
1111	4	-0.006822727037189225
1111	5	-0.11882059989669358
1111	6	-0.008863162532328772
1111	7	-0.1068681053196272
1111	8	-0.08264714233285986
1111	9	-1.747271602471045
1111	10	-0.4004393882002688
1111	11	-0.19408818506546766
1111	12	-0.09071033317508181
1111	13	-0.05119748250109524
1111	14	0.784327555990366
1111	15	0.23194173293683207
1111	16	0.2658853088827655
1111	17	-0.09105172482528445
1111	18	0.10632639009410383
1111	19	-0.20217597500312223
1111	20	0.19093567919336607
1111	21	-0.5337952820923277
1111	22	-0.22778434541423564
1111	23	0.014972792409594149
1111	24	-0.5108628527758654
1111	25	-2.0107864443402548
1112	1	0.12215459654595051
1112	2	0.36254496613009124
1112	3	-0.08682239710918083
1112	4	0.2649110534434982
1112	5	0.07057812811402897
1112	6	0.06020561376537614
1112	7	0.14308783612339226
1112	8	-0.018402208382092056
1112	9	-0.37906655318734295
1112	10	0.37013333070638765
1112	11	0.0975252496255763
1112	12	-0.1245063404998703
1112	13	-0.0626607258857419
1112	14	0.3637370630014516
1112	15	0.1421041625237649
1112	16	0.13467729509381599
1112	17	-3.741939962018577
1112	18	-1.044595403072357
1112	19	-1.1943127957172308
1112	20	-0.14959280185908597
1112	21	-0.5284247824370354
1112	22	-1.6240374817148648
1112	23	-1.3185960677469168
1112	24	-0.3337523843954955
1112	25	-0.10647485664716783
1113	1	-0.3683028779785893
1113	2	-0.15597074264530164
1113	3	-0.1724541470759533
1113	4	-0.5137586347247733
1113	5	-0.2612091866288466
1113	6	-0.25695796986763497
1113	7	-0.29912947094504005
1113	8	-0.2736780855472887
1113	9	0.13859920344013688
1113	10	0.29584452609390866
1113	11	-0.16052807317664275
1113	12	-0.09200989070073717
1113	13	-0.35267764680284586
1113	14	-1.508982745761699
1113	15	-1.7924757466501617
1113	16	-0.4887519583707629
1113	17	-0.34597987861446455
1113	18	-0.5439788281003572
1113	19	-0.21771541969796004
1113	20	0.13792978437351877
1113	21	-1.8126856618197635
1113	22	-2.0944497432821336
1113	23	-0.6854668223331188
1113	24	-2.314898757474083
1113	25	-0.69219044899598
1114	1	0.07296316233171751
1114	2	0.1801273928742262
1114	3	-0.029707184937667108
1114	4	0.2936008498685183
1114	5	0.09214685319517457
1114	6	0.16836269331302134
1114	7	0.17689185630302973
1114	8	0.09868953004592385
1114	9	0.4820940816968218
1114	10	0.31070156852671715
1114	11	0.25485356892777844
1114	12	0.2397389286237228
1114	13	0.16651887882160565
1114	14	1.063480232086512
1114	15	0.41301730874647546
1114	16	0.4028698400033264
1114	17	-0.3175779633599305
1114	18	-0.14671431601755813
1114	19	-0.48787620539426513
1114	20	-1.6521043951463739
1114	21	-0.8647761762252983
1114	22	-1.4153102097455132
1114	23	-2.9109367862620843
1114	24	-2.0464656304788775
1114	25	-1.532794513637782
1115	1	0.20553834967281048
1115	2	0.23561185917512972
1115	3	0.05364426918606924
1115	4	0.3017795989206604
1115	5	0.3011468225292262
1115	6	0.16746780504860226
1115	7	0.2831448532029839
1115	8	0.23741951354206492
1115	9	0.46642029495343706
1115	10	0.6691374237165135
1115	11	0.3392009695700864
1115	12	0.25299951395313514
1115	13	0.19153811473756832
1115	14	0.8466907622172073
1115	15	0.3633067535254568
1115	16	0.29736537113763006
1115	17	0.22692634938817946
1115	18	0.5440129270575085
1115	19	-0.058546939481423976
1115	20	0.5309103607526424
1115	21	-0.4394223329450426
1115	22	0.5676621713820964
1115	23	0.660695046669801
1115	24	0.6454753570763216
1115	25	0.6058758803360789
1116	1	0.14454585099735695
1116	2	0.4054137518600278
1116	3	0.1778714472874654
1116	4	0.48302014214448263
1116	5	0.4308513449394411
1116	6	0.30009122812371913
1116	7	0.3001248165998476
1116	8	0.2334360756429561
1116	9	0.4769205554303042
1116	10	0.4466793574231005
1116	11	0.29651890340214493
1116	12	0.28357954887885484
1116	13	-0.2847856378885112
1116	14	0.7010331399618437
1116	15	0.45033483524972695
1116	16	0.4198526132263325
1116	17	0.6202575365148988
1116	18	0.5956034553823244
1116	19	0.6665884726891207
1116	20	0.47894748518353225
1116	21	-0.041525001741888445
1116	22	-0.03736783630264995
1116	23	-0.9260672736471847
1116	24	-0.3623591494257141
1116	25	-0.9987069015295518
1201	1	0.4770989942552255
1201	2	0.6510320658224771
1201	3	0.5130313002980742
1201	4	0.4048247963900877
1201	5	0.40402497667430737
1201	6	0.28962941670375386
1201	7	0.36033968789050164
1201	8	0.3126208470263947
1201	9	0.5752244037162484
1201	10	0.8826862006783358
1201	11	0.5356986754012709
1201	12	0.6216830310730117
1201	13	0.5444388507133908
1201	14	1.1242170027712035
1201	15	0.8053501089812016
1201	16	0.5132306913360694
1201	17	0.6300184320272066
1201	18	0.6797744268353725
1201	19	0.9404995161407896
1201	20	0.9967879181773824
1201	21	0.8418101797474478
1201	22	0.992255008788355
1201	23	0.5748239407522533
1201	24	0.95000760050835
1201	25	1.1065230273823232
1202	1	0.48279800747517143
1202	2	0.8724371318417816
1202	3	0.6679453636177755
1202	4	0.5342532285592156
1202	5	0.39544340302105496
1202	6	0.37344661567774295
1202	7	0.39452237148150715
1202	8	0.4773779321654388
1202	9	0.8845908325640934
1202	10	0.3065292136170825
1202	11	0.4951872947168354
1202	12	0.4690686692690562
1202	13	0.5221650451371289
1202	14	1.2667089455718497
1202	15	0.9213488457010257
1202	16	0.8225802905246908
1202	17	-1.1423934963011746
1202	18	0.3104941937886849
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2210	20	0.8711104922236714
2210	21	0.9430098509214133
2210	22	1.1057718079672298
2210	23	1.4174649753859683
2210	24	1.168108878570024
2210	25	1.1642206721535118
2211	1	0.12413628791068376
2211	2	-0.5785932325463286
2211	3	-0.06787763670572389
2211	4	0.21233839358338713
2211	5	0.24779975772553087
2211	6	0.14264447202150876
2211	7	0.13749946747985575
2211	8	0.30677889243499834
2211	9	0.6095770879670255
2211	10	0.730586462456877
2211	11	0.3038936617586856
2211	12	0.1811770147574734
2211	13	0.09304744699566925
2211	14	-0.8419224859295937
2211	15	-0.11240240017060188
2211	16	0.12549493750369578
2211	17	0.2151858618907383
2211	18	0.2972135184332245
2211	19	0.5486557639774148
2211	20	0.606650341259396
2211	21	0.5872373054167213
2211	22	-0.04944575392958939
2211	23	0.517318193800314
2211	24	-0.11590173511091706
2211	25	0.4055028159081857
2212	1	-0.03152621099820152
2212	2	-1.2069763355929943
2212	3	-0.3102047383948445
2212	4	0.11062144172521471
2212	5	0.1428696366797766
2212	6	0.11291014838454316
2212	7	0.1641095887900903
2212	8	0.0770064803233268
2212	9	-2.1009649373282855
2212	10	0.06457718671176593
2212	11	0.040767336661936326
2212	12	0.008191834129824827
2212	13	0.031141265634431647
2212	14	0.6095327439772049
2212	15	0.3251664004983752
2212	16	0.3605566836137868
2212	17	0.3310641125920526
2212	18	0.12777926526413097
2212	19	-0.5309036038358173
2212	20	0.24690871918183888
2212	21	0.24377474381161335
2212	22	-1.6952331039867516
2212	23	-0.2247203207087054
2212	24	0.32663578825398076
2212	25	-0.26473093008565485
2213	1	0.06709593819488191
2213	2	0.386282272629515
2213	3	0.07762925305491919
2213	4	0.30317096924586906
2213	5	0.2564324267933813
2213	6	0.24642923700761799
2213	7	0.09660754181930997
2213	8	0.10679679619179439
2213	9	0.5414845134386539
2213	10	0.4951428220178329
2213	11	-0.037221328145761845
2213	12	-0.001748089558986976
2213	13	0.036435258296442896
2213	14	-1.6994069889856576
2213	15	-0.4023346271239774
2213	16	-0.12312229992751611
2213	17	-2.0612672573450936
2213	18	-0.8198369109065098
2213	19	-0.4798437036884664
2213	20	0.16587482187523506
2213	21	-0.12600949946354945
2213	22	0.32204158951607553
2213	23	0.7737235654370664
2213	24	0.5651705533778539
2213	25	-0.9396816150868474
2214	1	0.11061371423474853
2214	2	0.06320480827074704
2214	3	-0.0030585537870919666
2214	4	0.3640846722682838
2214	5	0.27181414310912544
2214	6	0.23758109073968825
2214	7	0.244203908680511
2214	8	0.20622607411768
2214	9	0.5590356002385661
2214	10	0.5338911022438495
2214	11	-0.11401981850151799
2214	12	-0.12430643251727247
2214	13	-0.057511866632275144
2214	14	0.8359618347304476
2214	15	0.423434659378934
2214	16	0.38842975558341664
2214	17	0.3584635576882107
2214	18	0.21993854336542995
2214	19	-0.9837680200093573
2214	20	-0.032396282623672334
2214	21	0.29836244043050186
2214	22	0.47527483341404775
2214	23	1.009376763956361
2214	24	0.7112879822725731
2214	25	0.822950194496805
2215	1	-0.26732309194680326
2215	2	0.1327056438430816
2215	3	0.03436685115749327
2215	4	0.37160244459002456
2215	5	0.28682774093526575
2215	6	0.21318603635847921
2215	7	0.24750385278120746
2215	8	0.060396766050565986
2215	9	0.3260676432650982
2215	10	0.6091451680712865
2215	11	0.3007531721921808
2215	12	0.16692437776236346
2215	13	-0.01442525995806676
2215	14	0.7637018488724578
2215	15	0.24629805929101423
2215	16	0.36956601726846827
2215	17	0.41807975318273805
2215	18	0.3733192845170805
2215	19	-0.3194930178606725
2215	20	-1.627166230384418
2215	21	-1.8271392900693186
2215	22	-0.13552847620191244
2215	23	0.4385167741039595
2215	24	0.5778264687545231
2215	25	-0.1645840581344018
2216	1	-0.20058091795424815
2216	2	-0.37660945405876234
2216	3	-0.25643533172278055
2216	4	0.07607161542593843
2216	5	0.13311945954266946
2216	6	-0.04771506877546017
2216	7	0.02069267086479698
2216	8	-0.13720191545723992
2216	9	0.2072369878797673
2216	10	-1.623848146703267
2216	11	-0.49753532351282104
2216	12	-0.2548378112668743
2216	13	-0.1373383025528554
2216	14	-0.6604726578635649
2216	15	-0.483290843959189
2216	16	-0.05948820930121457
2216	17	0.23887440888965017
2216	18	0.00407229339861459
2216	19	-1.3375263406218068
2216	20	-1.0424492635879048
2216	21	-0.9581687370429393
2216	22	-0.8351230071627143
2216	23	-4.6502489638284885
2216	24	-2.235367243758695
2216	25	-1.282592732053189
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	13.0
4	41.0
5	62.0
6	88.0
7	402.0
8	192.0
9	515.0
10	1365.0
11	2687.0
12	4928.0
13	8085.0
14	11553.0
15	15107.0
16	17776.0
17	19761.0
18	21302.0
19	21875.0
20	22577.0
21	23000.0
22	23931.0
23	25549.0
24	27220.0
25	30518.0
26	33919.0
27	39389.0
28	46470.0
29	57778.0
30	71748.0
31	89732.0
32	119954.0
33	162792.0
34	223962.0
35	313939.0
36	453800.0
37	694056.0
38	1251654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.341273678517204	22.023061633122047	14.149737886201253	22.485926802159497
2	15.30926373422459	21.835769969330077	40.9446846794253	21.910281617020033
3	17.44258604924666	27.247868122289866	31.119211035823053	24.19033479264042
4	12.192246314515208	18.248786710827662	39.77276341108776	29.78620356356937
5	12.8792328337159	38.29400133213936	35.709650971666626	13.117114862478118
6	29.553346105620786	39.562095235464184	18.35146273618676	12.533095922728268
7	24.895772627426368	33.434963057794974	24.71619608403244	16.953068230746215
8	22.480490220280576	37.75910397734397	22.247131436102784	17.513274366272668
9	23.812187691354012	17.709426823619207	23.219457846918875	35.258927638107906
10	14.445197119285465	30.59876919852988	34.573578715177966	20.38245496700669
11	30.54376391311725	26.652117921463358	24.57945603539656	18.22466213002283
12	20.101935024501206	28.365990314191937	32.36408359339205	19.167991067914812
13	25.402803306667444	25.229942459649298	27.783158682712767	21.584095550970492
14	20.35050482694906	24.426267841515802	29.044930654235184	26.178296677299954
15	22.12449751353758	31.65091876721931	25.035836496424952	21.188747222818158
16	21.16157622268918	32.133420005807125	26.56483519805998	20.140168573443713
17	19.263339135167087	31.08226875157361	29.38355125396237	20.27084085929693
18	19.31393610937486	30.785216919406107	30.3330853638765	19.567761607342536
19	20.157110431572832	29.66950826952563	30.303923538651716	19.869457760249826
20	21.48808405314463	29.38766500191165	29.571011318475026	19.553239626468695
21	22.07007876642357	29.83755206965137	28.296623968510108	19.795745195414952
22	20.668147581706396	29.422148619545833	30.024001665964263	19.885702132783504
23	21.090870422583667	29.64854283201792	29.241930700010894	20.018656045387516
24	20.622383928524886	28.804308731619084	29.65688088697529	20.91642645288074
25	21.369137285436484	29.29898491126614	28.958500438876218	20.37337736442116
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2477.0
1	2477.0
2	3068.5
3	3660.0
4	3660.0
5	3660.0
6	5950.0
7	8240.0
8	8240.0
9	8240.0
10	13763.5
11	19287.0
12	19287.0
13	19287.0
14	32845.5
15	46404.0
16	46404.0
17	46404.0
18	72406.0
19	98408.0
20	98408.0
21	98408.0
22	136432.5
23	174457.0
24	174457.0
25	174457.0
26	221600.0
27	268743.0
28	268743.0
29	268743.0
30	316134.0
31	363525.0
32	363525.0
33	363525.0
34	399570.5
35	435616.0
36	435616.0
37	435616.0
38	456275.0
39	476934.0
40	476934.0
41	476934.0
42	471322.5
43	465711.0
44	465711.0
45	465711.0
46	438040.0
47	410369.0
48	410369.0
49	410369.0
50	383234.0
51	356099.0
52	356099.0
53	356099.0
54	317564.5
55	279030.0
56	279030.0
57	279030.0
58	239082.5
59	199135.0
60	199135.0
61	199135.0
62	161103.5
63	123072.0
64	123072.0
65	123072.0
66	92856.5
67	62641.0
68	62641.0
69	62641.0
70	44990.5
71	27340.0
72	27340.0
73	27340.0
74	18983.5
75	10627.0
76	10627.0
77	10627.0
78	7302.0
79	3977.0
80	3977.0
81	3977.0
82	2670.5
83	1364.0
84	1364.0
85	1364.0
86	903.0
87	442.0
88	442.0
89	442.0
90	284.0
91	126.0
92	126.0
93	126.0
94	83.5
95	41.0
96	41.0
97	41.0
98	29.5
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08205343609512153
2	0.019777249284279846
3	0.03176346097172218
4	0.03848616230946158
5	0.04711102332803421
6	0.020585015724085744
7	0.024467506031539892
8	0.041430601267463715
9	0.0415348291951806
10	0.027516172917258917
11	0.02569218418221335
12	0.020558958742156522
13	0.0294704465619506
14	0.043671501713376845
15	0.04090946162887927
16	0.02806336953777259
17	0.026708406477453026
18	0.032545170429598855
19	0.021470953109679308
20	0.019334280591483066
21	0.014982764609302915
22	0.02412876526646
23	0.023894252429097
24	0.023008315043503435
25	0.10652094212666141
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3837743.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.20115537743007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.20427146393608	68.27477898413362
2	10.669282793202363	16.90039048540323
3	1.9368667034825142	4.602062421836681
4	0.5170046114020007	1.6378945023399083
5	0.19595530407935663	0.7759943242710339
6	0.1076810036616878	0.511707594132417
7	0.06355092912186275	0.35233149082324877
8	0.052531537615022786	0.3328446778290186
9	0.039233487139364746	0.2796603759830909
>10	0.1899284821768454	2.7476660505721764
>50	0.013718099434662312	0.7570591517562887
>100	0.008687762236171324	1.2976283984893844
>500	7.5948511086236E-4	0.4159909769263679
>1k	4.6229522618796977E-4	0.8222267728340085
>5k	6.604217516970593E-5	0.29176379266948516
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	5769	0.1503227287496844	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5387	0.14036896165272142	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4476	0.11663105111519975	No Hit
GTACATAAGCAGTGGTATCAACGCA	4134	0.10771956329540565	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.605698192922246E-5	0.0	0.0	0.0
3	0.0	2.605698192922246E-5	0.0	0.0	0.0
4	0.0	2.605698192922246E-5	0.0	5.211396385844492E-5	0.0
5	0.0	2.605698192922246E-5	0.0	5.211396385844492E-5	0.0
6	0.0	2.605698192922246E-5	0.0	5.211396385844492E-5	0.0
7	0.0	2.605698192922246E-5	0.0	5.211396385844492E-5	0.0
8	0.0	2.605698192922246E-5	0.0	5.211396385844492E-5	0.0
9	2.605698192922246E-5	2.605698192922246E-5	0.0	1.8239887350455724E-4	0.0
10	2.605698192922246E-5	2.605698192922246E-5	0.0	2.084558554337797E-4	0.0
11	2.605698192922246E-5	2.605698192922246E-5	0.0	2.3451283736300216E-4	0.0
12	2.605698192922246E-5	2.605698192922246E-5	0.0	2.3451283736300216E-4	0.0
13	2.605698192922246E-5	2.605698192922246E-5	0.0	2.3451283736300216E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGC	60	0.0058843307	11.081813	6
AGGACCG	70	0.0014900828	10.85905	5
AGTACCG	75	0.0026452295	10.135114	5
TAGACAG	610	0.0	9.345904	5
CCGTACC	115	1.10929584E-4	9.088552	4
TAGTACC	275	1.8189894E-12	8.63788	4
AACCCGT	110	7.158122E-4	8.635179	6
CTAGAAC	400	0.0	8.551502	3
TATACTG	1150	0.0	8.51019	5
GGTATCA	4390	0.0	8.468504	1
GTCTAAG	330	0.0	8.355617	1
ATTAGAC	425	0.0	8.272041	3
GTCTTAC	290	9.094947E-12	8.1966505	1
CTAGTAC	290	9.094947E-12	8.191093	3
CATAAGC	1340	0.0	8.154418	4
TCAGGAC	455	0.0	8.144288	3
TAGTACT	455	0.0	8.144288	4
GTCTAGC	165	9.641222E-6	8.067492	1
GTCTTAA	460	0.0	8.061228	1
TGTCGCC	330	0.0	8.059396	10
>>END_MODULE